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Information on EC 2.7.7.68 - 2-phospho-L-lactate guanylyltransferase and Organism(s) Methanocaldococcus jannaschii and UniProt Accession Q58297

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EC Tree
IUBMB Comments
This enzyme is involved in the biosynthesis of coenzyme F420, a redox-active cofactor, in all methanogenic archaea. cf. EC 2.7.7.105, phosphoenolpyruvate guanylyltransferase and EC 2.7.7.106, 3-phospho-(R)-glycerate guanylyltransferase.
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Methanocaldococcus jannaschii
UNIPROT: Q58297
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The taxonomic range for the selected organisms is: Methanocaldococcus jannaschii
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
mj0887, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
GTP:2-phospho-L-lactate guanylyltransferase
This enzyme is involved in the biosynthesis of coenzyme F420, a redox-active cofactor, in all methanogenic archaea. cf. EC 2.7.7.105, phosphoenolpyruvate guanylyltransferase and EC 2.7.7.106, 3-phospho-(R)-glycerate guanylyltransferase.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2S)-2-phospholactate + dGTP
(2S)-lactyl-2-diphospho-5'-(2'-deoxy)guanosine + diphosphate
show the reaction diagram
specific activity is about 95% of the activity with GTP
-
-
?
(2S)-2-phospholactate + gamma-thiolGTP
?
show the reaction diagram
specific activity is about 65% of the activity with GTP
-
-
?
(2S)-2-phospholactate + GTP
lactyl-2-diphospho-5'-guanosine + diphosphate
show the reaction diagram
(2S)-2-phospholactate + ITP
lactyl-2-diphospho-5'-inosine + diphosphate + diphosphate
show the reaction diagram
specific activity is about 35% of the activity with GTP
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2S)-2-phospholactate + GTP
lactyl-2-diphospho-5'-guanosine + diphosphate
show the reaction diagram
third step of coenzyme F420 biosynthesis
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
1 mM, 16fold activation
Fe2+
1 mM, 10fold activation
Mg2+
1 mM, 23fold activation
Mn2+
1 mM, 6fold activation
Ni2+
1 mM, 8fold activation
Zn2+
1 mM, 3fold activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.036
(2S)-2-phospholactate
pH 7.5, 70°C
0.056
GTP
pH 7.5, 70°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.3
assay with gamma-thioGTP
additional information
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000
2 * 25000, recombinant enzyme, SDS-PAGE
50000
recombinant enzyme, gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 25000, recombinant enzyme, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100
10 min, 62% loss of activity
90
10 min, completely stable
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Grochowski, L.L.; Xu, H.; White, R.H.
Identification and characterization of the 2-phospho-L-lactate guanylyltransferase involved in coenzyme F420 biosynthesis
Biochemistry
47
3033-3037
2008
Methanocaldococcus jannaschii (Q58297), Methanocaldococcus jannaschii
Manually annotated by BRENDA team