Information on EC 2.7.7.68 - 2-phospho-L-lactate guanylyltransferase

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The expected taxonomic range for this enzyme is: Methanocaldococcus jannaschii

EC NUMBER
COMMENTARY hide
2.7.7.68
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RECOMMENDED NAME
GeneOntology No.
2-phospho-L-lactate guanylyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(2S)-2-phospholactate + GTP = (2S)-lactyl-2-diphospho-5'-guanosine + diphosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
factor 420 biosynthesis
Methane metabolism
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
GTP:2-phospho-L-lactate guanylyltransferase
This enzyme is involved in the biosynthesis of coenzyme F420, a redox-active cofactor found in all methanogenic archaea, as well as some eubacteria.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2S)-2-phospholactate + dGTP
(2S)-lactyl-2-diphospho-5'-(2'-deoxy)guanosine + diphosphate
show the reaction diagram
specific activity is about 95% of the activity with GTP
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?
(2S)-2-phospholactate + gamma-thiolGTP
?
show the reaction diagram
specific activity is about 65% of the activity with GTP
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?
(2S)-2-phospholactate + GTP
lactyl-2-diphospho-5'-guanosine + diphosphate
show the reaction diagram
(2S)-2-phospholactate + ITP
lactyl-2-diphospho-5'-inosine + diphosphate + diphosphate
show the reaction diagram
specific activity is about 35% of the activity with GTP
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(2S)-2-phospholactate + GTP
lactyl-2-diphospho-5'-guanosine + diphosphate
show the reaction diagram
Q58297
third step of coenzyme F420 biosynthesis
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
1 mM, 16fold activation
Fe2+
1 mM, 10fold activation
Mg2+
1 mM, 23fold activation
Mn2+
1 mM, 6fold activation
Ni2+
1 mM, 8fold activation
Zn2+
1 mM, 3fold activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.036
(2S)-2-phospholactate
pH 7.5, 70°C
0.056
GTP
pH 7.5, 70°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.3
assay with gamma-thioGTP
additional information
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000
2 * 25000, recombinant enzyme, SDS-PAGE
50000
recombinant enzyme, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 25000, recombinant enzyme, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
90
10 min, completely stable
100
10 min, 62% loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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