Information on EC 2.7.7.67 - CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase

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The expected taxonomic range for this enzyme is: Archaea, Eukaryota

EC NUMBER
COMMENTARY hide
2.7.7.67
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RECOMMENDED NAME
GeneOntology No.
CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate = diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
CDP-archaeol biosynthesis
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lipid metabolism
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Glycerophospholipid metabolism
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SYSTEMATIC NAME
IUBMB Comments
CTP:2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate cytidylyltransferase
This enzyme catalyses one of the steps in the biosynthesis of polar lipids in archaea, which are characterized by having an sn-glycerol 1-phosphate backbone rather than an sn-glycerol 3-phosphate backbone as is found in bacteria and eukaryotes [1]. The enzyme requires Mg2+ and K+ for maximal activity [1].
CAS REGISTRY NUMBER
COMMENTARY hide
329791-09-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CTP + 1,2-bis-(O-geranylgeranyl)-sn-glycerol 3-phosphate
diphosphate + CDP-1,2-bis-(O-geranylgeranyl)-sn-glycerol
show the reaction diagram
CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
show the reaction diagram
dCTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
diphosphate + dCDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
show the reaction diagram
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?
additional information
?
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no substrates: ATP, GTP, TTP
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CTP + 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
diphosphate + CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
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the enzyme requires Mg2+ (1-2 mM) and K+ (0.5 mM) for maximal activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
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0.1%, 96% inhibition
additional information
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dithiothreitol shows no effect on the activity
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12
2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
pH 7.5, 65°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.55
2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate
pH 7.5, 65°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 9
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pH 8.0: about 60% of maximal activity, pH 9.0: about 55% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45 - 65
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45°C: about 30% of maximal activity, 65°C: about 55% of maximal activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing and thawing causes a small loss (5–10%) of activity
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, activity in membrane fraction is stable for several months
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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