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Information on EC 2.7.7.64 - UTP-monosaccharide-1-phosphate uridylyltransferase and Organism(s) Pisum sativum and UniProt Accession Q5W915

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Pisum sativum
UNIPROT: Q5W915 not found.
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The taxonomic range for the selected organisms is: Pisum sativum
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
atusp, psusp, uspase, udp-galactose/glucose pyrophosphorylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
UDP-sugar pyrophosphorylase
-
CAS REGISTRY NUMBER
COMMENTARY hide
223918-15-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
diphosphate + UDP-D-glucose
UTP + D-glucose 1-phosphate
show the reaction diagram
-
-
-
r
UTP + a monosaccharide 1-phosphate
diphosphate + UDP-monosaccharide
show the reaction diagram
the enzyme catalyzes the formation of various UDP-sugars at the end of salvage pathways
-
-
?
UTP + arabinose 1-phosphate
diphosphate + UDP-arabinose
show the reaction diagram
70.5% of enzyme activity with glucose 1-phosphate
-
-
r
UTP + D-galactose 1-phosphate
diphosphate + UDP-galactose
show the reaction diagram
UTP + D-glucose 1-phosphate
diphosphate + UDP-glucose
show the reaction diagram
UTP + D-glucuronic acid 1-phosphate
diphosphate + UDP-D-glucuronic acid
show the reaction diagram
71.3% of enzyme activity with glucose 1-phosphate
-
-
r
UTP + D-glucuronic acid 1-phosphate
diphosphate + UDP-glucuronic acid
show the reaction diagram
-
-
-
?
UTP + D-xylose 1-phosphate
diphosphate + UDP-alpha-D-xylopyranose
show the reaction diagram
-
-
-
?
UTP + D-xylose 1-phosphate
diphosphate + UDP-xylose
show the reaction diagram
35.9% of enzyme activity with glucose 1-phosphate
-
-
r
UTP + L-arabinose 1-phosphate
diphosphate + UDP-beta-L-arabinopyranose
show the reaction diagram
-
-
-
?
UTP + N-acetyl-D-glucosamine 1-phosphate
diphosphate + UDP-N-acetylglucosamine
show the reaction diagram
4.3% of enzyme activity with glucose 1-phosphate
-
-
r
UTP + D-galacturonic acid 1-phosphate
diphosphate + UDP-galacturonic acid
show the reaction diagram
-
equilibrium constant is 0.24
-
-
r
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UTP + a monosaccharide 1-phosphate
diphosphate + UDP-monosaccharide
show the reaction diagram
the enzyme catalyzes the formation of various UDP-sugars at the end of salvage pathways
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
75% activity in comarison with Mg2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.96
arabinose 1-phosphate
pH 7.0, 35°C
0.58
D-galactose 1-phosphate
pH 7.0, 35°C
0.34
D-glucose 1-phosphate
pH 7.0, 35°C
0.48
D-glucuronic acid 1-phosphate
pH 7.0, 35°C
1.98
D-xylose 1-phosphate
pH 7.0, 35°C
0.25
diphosphate
pH 7.0, 35°C
0.96
L-arabinose 1-phosphate
pH 7.0, 35°C
0.34
UDP-D-glucose
pH 7.0, 35°C
0.34
UDP-glucose
pH 7.0, 35°C
0.048
UTP
1.15
D-galacturonic acid 1-phosphate
-
pH 7.0, 35°C
0.7
diphosphate
-
pH 7.0, 35°C
1.26
UDP-galacturonic acid
-
pH 7.0, 35°C
2.27
UTP
-
pH 7.0, 35°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
UDP-L-arabinose pyrophosphorylase, pH 7.0, 35°C
95
recombinant UDP-glucose pyrophosphorylase, pH 7.0, 35°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
pH 6.5-7.5, formation of UDP-glucose
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9
-
pH 5.0: about 75% of maximal activity, pH 9.0: about 50% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.82
calculated
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
USP_PEA
600
0
66177
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
66040
x * 66040, calculated from sequence
67000
x * 67000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8
80% loss of activity below pH 5.0
669277
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate fractionation, ion exchange chromatography (DEAE), hydrophobic interaction chromatography, gel filtration and ion exchange chromatography (DEAE), recombinant protein: immobilized metal ion affinity chromatography, splitting off the fused thioredoxin and His-tag, ion exchange chromatography (DEAE)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
His-tagged thioredoxin fusion protein expressed in Escherichia coli BL21gold strain
expression in Escherichia coli
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
-
the enzyme is useful for the highly efficient production of UDP-galacturonic acid for studies on pectin biosynthesis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ohashi, T.; Cramer, N.; Ishimizu, T.; Hase, S.
Preparation of UDP-galacturonic acid using UDP-sugar pyrophosphorylase
Anal. Biochem.
352
182-187
2006
Pisum sativum
Manually annotated by BRENDA team
Kotake, T.; Yamaguchi, D.; Ohzono, H.; Hojo, S.; Kaneko, S.; Ishida, H.K.; Tsumuraya, Y.
UDP-sugar pyrophosphorylase with broad substrate specificity toward various monosaccharide 1-phosphates from pea sprouts
J. Biol. Chem.
279
45728-45736
2004
Pisum sativum (Q5W915), Pisum sativum
Manually annotated by BRENDA team