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d(Ap4T) + RNAn
?
-
primer elongation
-
-
?
d(TP4C) + RNAn
?
-
primer elongation
-
-
?
d(Tp4G) + RNAn
?
-
primer elongation
-
-
?
d(Tp4T) + RNAn
?
-
primer elongation
-
-
?
dTTP + RNAn
?
-
primer elongation
-
-
?
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
additional information
?
-
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
-
-
-
-
?
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
-
-
-
?
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
-
the enzyme requires DNA as template
-
-
?
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
-
a kind of transcription complex is formed during RNA polymerase catalysed synthesis of the M13 bacteriophage replication primer. The complex contains an overextended RNADNA hybrid bound in the RNA-polymerase through that is normally occupied by downstream double-stranded DNA, thus leaving the 30 end of the RNA available for interaction with DNA polymerase
-
-
?
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
-
enzyme is responsible for transcription in bacteria
-
-
?
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
-
regulation by anions, overview
-
-
?
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
-
RNAP is an exceptionally complex enzyme that can be thought of as the engine of gene expression, synthesis of RNA transcripts of many thousands of nucleotides without dissociation. Energy, in the form of nucleoside triphosphates, fuels the synthesis of an RNA polymer complementary to specific regions of the DNA template. Like all macromolecular synthesis, RNA synthesis can be divided into three general phases: initiation, elongation, and termination. Importantly, each of these phases can be a target of regulation. Promoter recognition, binding at the extended promoter recognition region, and transcript initiation, RNAP prefers to initiate transcription within a narrow window located between 6 and 9 bp downstream of the -10 element, promoter clearance and elongation, termination and recycling, mechanisms and regulation , overview. The process of start site selection can be governed by the availability of either the +1 or the +2 NTP, depending on the promoter
-
-
?
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
-
template is DNA
-
-
?
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
-
template is DNA, native or denatured, method evaluation: specificity and extent of transcription depends strongly on the quality of the DNA preparation, the strength of the promoter and terminator sequences, and the kind and concentration of mono- and divalent cations in the reaction mixture
-
-
?
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
-
template is supercoiled DNA
-
-
?
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
the alpha subunit C-terminal domain of Escherichia coli RNA polymerase (alphaCTD) recognizes the upstream promoter(UP) DNA element via its characteristic minor groove shape and electrostatic potential
-
-
?
additional information
?
-
-
dinucleoside teraphosphates are more potent substrates than dinucleoside triphosphates and dinucleoside pentaphosphates
-
-
?
additional information
?
-
-
multi-subunit DNA-dependent RNA polymerases synthesize RNA molecules thousands of nucleotides long. The reiterative reaction of nucleotide condensation occurs at rates of tens of nucleotides per second, invariably linked to the translocation of the enzyme along the DNA template, or threading of the DNA and the nascent RNA molecule through the enzyme. Reiteration of the nucleotide addition/translocation cycle without dissociation from the DNA and RNA requires both isomorphic and metamorphic conformational flexibility of a magnitude substantial enough to accommodate the requisite molecular motions
-
-
?
additional information
?
-
-
RNAP adds nucleotides to the 3'-end of the growing RNA and translocates reiteratively, in single nucleotide steps. Translocation mechanism models, concerning conformational changes, allosteric effects and isomerization, and model evaluation, overview
-
-
?
additional information
?
-
-
the core enzyme, which lacks the sigma subunit, synthesizes short transcripts relatively uniformly on the DNA template in the presence of high concentrations of random primers and low NTP concentrations
-
-
?
additional information
?
-
-
dsDNA templates used for activity are T7A1_763, T7A1_437, T7A1_149, pcDNA3.1, pGEM, T-phage DNA, Escherichia coli DNA, calf thymus DNA, poly(dA-dT), and Kool NC-45
-
-
?
additional information
?
-
-
RNA polymerase binds multiple sites in the ehxCABD gene regulatory region. At the Escherichia coli ehxCABD operon, RNA polymerase is unable to distinguish between the promoter -10 element and similar overlapping sequences. RNA polymerase competes with itself for binding to AT-rich sequences overlapping PehxCABD, correct positioning of RNA polymerase at PehxCABD requires H-NS
-
-
?
additional information
?
-
the subunits interact with recombinant His6-tagged CedA, a multi-copy suppressor which represses the dnaAcos inhibition of cell division. Determination of the binding site of CedA for RNA polymerase. The N-terminus of CedA is necessary for a tight interaction
-
-
?
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nucleoside triphosphate + RNAn
diphosphate + RNAn+1
additional information
?
-
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
-
-
-
?
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
-
a kind of transcription complex is formed during RNA polymerase catalysed synthesis of the M13 bacteriophage replication primer. The complex contains an overextended RNADNA hybrid bound in the RNA-polymerase through that is normally occupied by downstream double-stranded DNA, thus leaving the 30 end of the RNA available for interaction with DNA polymerase
-
-
?
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
-
enzyme is responsible for transcription in bacteria
-
-
?
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
-
regulation by anions, overview
-
-
?
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
-
RNAP is an exceptionally complex enzyme that can be thought of as the engine of gene expression, synthesis of RNA transcripts of many thousands of nucleotides without dissociation. Energy, in the form of nucleoside triphosphates, fuels the synthesis of an RNA polymer complementary to specific regions of the DNA template. Like all macromolecular synthesis, RNA synthesis can be divided into three general phases: initiation, elongation, and termination. Importantly, each of these phases can be a target of regulation. Promoter recognition, binding at the extended promoter recognition region, and transcript initiation, RNAP prefers to initiate transcription within a narrow window located between 6 and 9 bp downstream of the -10 element, promoter clearance and elongation, termination and recycling, mechanisms and regulation , overview. The process of start site selection can be governed by the availability of either the +1 or the +2 NTP, depending on the promoter
-
-
?
nucleoside triphosphate + RNAn
diphosphate + RNAn+1
-
template is DNA
-
-
?
additional information
?
-
-
multi-subunit DNA-dependent RNA polymerases synthesize RNA molecules thousands of nucleotides long. The reiterative reaction of nucleotide condensation occurs at rates of tens of nucleotides per second, invariably linked to the translocation of the enzyme along the DNA template, or threading of the DNA and the nascent RNA molecule through the enzyme. Reiteration of the nucleotide addition/translocation cycle without dissociation from the DNA and RNA requires both isomorphic and metamorphic conformational flexibility of a magnitude substantial enough to accommodate the requisite molecular motions
-
-
?
additional information
?
-
-
RNA polymerase binds multiple sites in the ehxCABD gene regulatory region. At the Escherichia coli ehxCABD operon, RNA polymerase is unable to distinguish between the promoter -10 element and similar overlapping sequences. RNA polymerase competes with itself for binding to AT-rich sequences overlapping PehxCABD, correct positioning of RNA polymerase at PehxCABD requires H-NS
-
-
?
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(S)-2-((1-amino-1-oxo-3-phenylpropan-2-ylamino)methyl)-3-(4-amino phenoxy)-5-methoxy phenyl acetate
-
-
(S)-2-((1-amino-1-oxo-3-phenylpropan-2-ylamino)methyl)-5-methoxy-3-(4-nitrophenoxy)phenyl acetate
-
-
(S)-2-((1-amino-3-(4-hydroxyphenyl)-1-oxopropan-2-ylamino)methyl)-3-(4-aminophenoxy)-5-methoxyphenyl acetate
-
-
(S)-2-((1-amino-3-(4-hydroxyphenyl)-1-oxopropan-2-ylamino)methyl)-5-methoxy-3-(4-nitrophenoxy)phenyl acetate
-
-
1,3-dimethoxy-5-(4-nitrophenoxy) benzene
-
-
1-[2-[3-(4-Chloro-3-trifluoromethylphenyl)ureido]-4-trifluoromethyl phenoxy]-4,5-dichlorobenzene sulfonic acid
-
-
2,4-dimethoxy-6-(4-nitrophenoxy) benzaldehyde
-
-
2-([[(1S)-2-amino-1-(4-hydroxybenzyl)-2-oxoethyl]amino]methyl)-5-methoxy-3-(4-nitrophenoxy)phenyl acetate
-
-
2-acetyl-3-hydroxy-5-methoxyphenyl acetate
-
-
2-acetyl-5-methoxy-3-(4-nitrophenoxy)phenyl acetate
-
-
2-formyl-5-methoxy-3-(4-nitrophenoxy)phenyl acetate
-
-
2-hydroxy-4-methoxy-6-(4-nitrophenoxy) benzaldehyde
-
-
4-[2-([[(1S)-2-amino-1-(4-hydroxybenzyl)ethyl]amino]methyl)-5-methoxy-3-(2-oxopropyl)benzyl]benzaldehyde
-
-
CBR703
-
the IC50s values are significantly decreased with template Kool NC-45, or increased with template poly(dA-dT)
corallopyronin
-
inhibition is not affected by template Kool NC-45
Cordycepin triphosphate
-
-
d(Ap4C)
-
d(Ap4T), d(Ap4C) and d(Ap4G) inhibit the incorporation of dATP into DNA less effectively than d(Ap4T), d(Tp4T) and d(Tp4C) the dTTP incorporation
d(Ap4G)
-
d(Ap4T), d(Ap4C) and d(Ap4G) inhibit the incorporation of dATP into DNA less effectively than d(Ap4T), d(Tp4T) and d(Tp4C) the dTTP incorporation
d(Ap4T)
-
d(Ap4T), d(Ap4C) and d(Ap4G) inhibit the incorporation of dATP into DNA less effectively than d(Ap4T), d(Tp4T) and d(Tp4C) the dTTP incorporation
d(Tp4C)
-
d(Ap4T), d(Ap4C) and d(Ap4G) inhibit the incorporation of dATP into DNA less effectively than d(Ap4T), d(Tp4T) and d(Tp4C) the dTTP incorporation
d(Tp4T)
-
d(Ap4T), d(Ap4C) and d(Ap4G) inhibit the incorporation of dATP into DNA less effectively than d(Ap4T), d(Tp4T) and d(Tp4C) the dTTP incorporation
etnangien
-
from the myxobacterium Sorangium cellulosum, a poly-unsaturated 22-membered polyketide macrolide, inhibits bacterial RNA polymerase, shows no cross-resistance to rifampicin, poor inhibition
etnangien methyl ester
-
weak inhibition
Exotoxin of Bacillus thuringiensis
-
-
-
myxopyronin
-
an alpha-pyrone antibiotic, targets the RNAP switch region, which is the hinge that mediates opening and closing of the RNAP active-center cleft. Lower values for inhibition by myxopyronin in the presence of template Kool NC-45
protein gp76
-
the Thermus phage protein gp76 inhibits Escherichia coli RNAP highlighting the template-DNA binding site as a target site for developing antibacterial agents
-
RBL-1
-
oligonucleotide, efficiently inhibits
-
additional information
-
despite relatively high overall sequence and structural homology between bacterial and mammalian core RNAP enzymes, there are sufficient differences between the enzyme classes for exploitation in the discovery of selective bacterial inhibitors
-
additional information
-
synthesis of simplified etnangien analogues and analysis of their antimicrobial activities, overview
-
additional information
-
the use of dsDNA templates containing classical promoters has only a negligible effects on the potency of enzyme inhibitors
-
additional information
-
FLiZ antagonize sigmaS-dependent gene expression in Escherichia coli. FliZ is an abundant DNA-binding protein and a global regulatory protein under the control of the flagellar master regulator FlhDC. It inhibits gene expression mediated by sigmaS by recognizing operator sequences that resemble the -10 region of sigmaS-dependent promoter. FLiZ plays a pivotal role in the decision between alternative life-styles, i.e. FlhDC-controlled flagellum-based motility or pS-dependent curli fimbriae-mediated adhesion and biofilm formation. FliZ is a global repressor with a DNA sequence specificity overlapping that of sigmaScontaining RNA polymerase, mechanism, overview
-
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Zalenskaya, K.; Lee, J.; Gujuluva, C.N.; Shin, Y.K.; Slutsky, M.; Goldfarb, A.
Recombinant RNA polymerase: inducible overexpression, purification and assembly of Escherichia coli rpo gene products
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89
7-12
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Escherichia coli
brenda
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Structure and function of DNA-dependent RNA-polymerase
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23
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1971
Escherichia coli
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Imburgio, D.; Anikin, M.; McAllister, W.T.
Effects of substitutions in a conserved DX2GR sequence motif, found in many DNA-dependent nucleotide polymerases, on transcription by T7 RNA polymerase
J. Mol. Biol.
319
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2002
Escherichia phage T7, Escherichia coli
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Conformation and DNA binding properties of a single-stranded DNA binding region of sigma 70 subunit from Escherichia coli RNA polymerase are modulated by an interaction with the core enzyme
Biochemistry
37
3312-3320
1998
Escherichia coli
brenda
Kuhlman, P.; Duff, H.L.; Galant, A.
A fluorescence-based assay for multisubunit DNA-dependent RNA polymerases
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324
183-190
2004
Escherichia coli
brenda
Tan, X.X.; Chen, Y.
A novel genomic approach identifies bacterial DNA-dependent RNA polymerase as the target of an antibacterial oligodeoxynucleotide, RBL1
Biochemistry
44
6708-6714
2005
Escherichia coli
brenda
King, R.A.; Markov, D.; Sen, R.; Severinov, K.; Weisberg, R.A.
A conserved zinc binding domain in the largest subunit of DNA-dependent RNA polymerase modulates intrinsic transcription termination and antitermination but does not stabilize the elongation complex
J. Mol. Biol.
342
1143-1154
2004
Escherichia coli
brenda
Skoblov, A.Y.; Sosunov, V.V.; Victorova, L.S.; Skoblov, Y.S.; Kukhanova, M.K.
Substrate properties of dinucleoside 5',5''-oligophosphates in the reactions catalyzed by HIV reverse transcriptase, E. coli DNA polymerase I, and E. coli RNA polymerase
Russ. J. Bioorg. Chem.
31
48-57
2005
Escherichia coli
-
brenda
Chopra, I.
Bacterial RNA polymerase: a promising target for the discovery of new antimicrobial agents
Curr. Opin. Investig. Drugs
8
600-607
2007
Escherichia coli
brenda
Zenkin, N.; Naryshkina, T.; Kuznedelov, K.; Severinov, K.
The mechanism of DNA replication primer synthesis by RNA polymerase
Nature
439
617-620
2006
Escherichia coli
brenda
Gralla, J.D.; Huo, Y.X.
Remodeling and activation of Escherichia coli RNA polymerase by osmolytes
Biochemistry
47
13189-13196
2008
Escherichia coli
brenda
Menche, D.; Li, P.; Irschik, H.
Design, synthesis and biological evaluation of simplified analogues of the RNA polymerase inhibitor etnangien
Bioorg. Med. Chem. Lett.
20
939-941
2009
Corynebacterium glutamicum, Saccharomyces cerevisiae, Escherichia coli, Micrococcus luteus, Staphylococcus aureus, Mycolicibacterium phlei, Gordonia rubripertincta
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Svetlov, V.; Nudler, E.
Macromolecular micromovements: how RNA polymerase translocates
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19
701-707
2009
Saccharomyces cerevisiae, Escherichia coli, Thermus thermophilus, Saccharolobus solfataricus, Thermus aquaticus
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Paschal, B.M.; McReynolds, L.A.; Noren, C.J.; Nichols, N.M.
RNA polymerases
Curr. Protoc. Mol. Biol.
Chapter 3
Unit3.8
2008
Enterobacteria phage T3, Escherichia phage T7, Escherichia coli, Zindervirus SP6
brenda
Helmann, J.D.
RNA polymerase: a nexus of gene regulation
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47
1-5
2009
Bacillus subtilis, Escherichia coli
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Agarwal, A.; Johnson, A.; Fishwick, C.
Synthesis of de novo designed small-molecule inhibitors of bacterial RNA polymerase
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64
10049-10054
2008
Escherichia coli, Thermus aquaticus
-
brenda
Kwapisz, M.; Beckouet, F.; Thuriaux, P.
Early evolution of eukaryotic DNA-dependent RNA polymerases
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24
211-215
2008
Saccharomyces cerevisiae, Cenarchaeum symbiosum, Escherichia coli, Emiliania huxleyi, Methanocaldococcus jannaschii, Pyrococcus furiosus, Sulfolobus acidocaldarius, Saccharolobus solfataricus, Nanoarchaeum equitans, Caldivirga maquilingensis, Nitrosopumilus maritimus, Thermofilum pendens
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Haupenthal, J.; Huesecken, K.; Negri, M.; Maurer, C.K.; Hartmann, R.W.
Influence of DNA template choice on transcription and inhibition of Escherichia coli RNA polymerase
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56
4536-4539
2012
Escherichia coli
brenda
Pesavento, C.; Hengge, R.
The global repressor FliZ antagonizes gene expression by sigmaS-containing RNA polymerase due to overlapping DNA binding specificity
Nucleic Acids Res.
40
4783-4793
2012
Escherichia coli
brenda
Abe, Y.; Fujisaki, N.; Miyoshi, T.; Watanabe, N.; Katayama, T.; Ueda, T.
Functional analysis of CedA based on its structure: residues important in binding of DNA and RNA polymerase and in the cell division regulation
J. Biochem.
159
217-223
2016
Escherichia coli (P0A8T7 AND P0A8V2 AND P0A7Z4)
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Singh, S.S.; Grainger, D.C.
H-NS can facilitate specific DNA-binding by RNA polymerase in AT-rich gene regulatory regions
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9
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2013
Escherichia coli
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The RNA polymerase alpha subunit recognizes the DNA shape of the upstream promoter element
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59
4523-4532
2020
Escherichia coli (P0A7Z4), Escherichia coli K12 (P0A7Z4)
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Structures of bacterial RNA polymerase complexes reveal the mechanism of DNA loading and transcription initiation
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70
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2018
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Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA
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Escherichia coli
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Transcriptional bursting is intrinsically caused by interplay between RNA polymerases on DNA
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7
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2016
Escherichia coli
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A Thermus phage protein inhibits host RNA polymerase by preventing template DNA strand loading during open promoter complex formation
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46
431-441
2018
Escherichia coli, Thermus thermophilus (Q5SHR6 AND Q8RQE9 AND Q8RQE8 AND Q8RQE7)
brenda