Catalyses DNA-template-directed extension of the 3'- end of an RNA strand by one nucleotide at a time. Can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. See also EC 2.7.7.19 (polynucleotide adenylyltransferase) and EC 2.7.7.48 (RNA-directed RNA polymerase).
rna polymerase ii, pol ii, t7 rna polymerase, rna polymerase i, pol iii, rna polymerase iii, pol i, rnapii, rnap ii, dna-dependent rna polymerase, more
Catalyses DNA-template-directed extension of the 3'- end of an RNA strand by one nucleotide at a time. Can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. See also EC 2.7.7.19 (polynucleotide adenylyltransferase) and EC 2.7.7.48 (RNA-directed RNA polymerase).
crystallization of the enzyme with DNA fragments, crystal growth is optimized using a nanoseeding technique, of the various crystals screened, one diffracts to 4.3 A resolution
Wojtas, M.; Peralta, B.; Ondiviela, M.; Mogni, M.; Bell, S.D.; Abrescia, N.G.
Archaeal RNA polymerase: the influence of the protruding stalk in crystal packing and preliminary biophysical analysis of the Rpo13 subunit
Biochem. Soc. Trans.
39
25-30
2011
Saccharolobus shibatae (B8YB53 and B8YB55 and B8YB54 and B8YB56 and B8YB57 and B8YB58 and B8YB59 and B8YB60 and B8YB61 and B8YB65 and B8YB62 and B8YB63 and B8YB64), Saccharolobus shibatae B12 (B8YB53 and B8YB55 and B8YB54 and B8YB56 and B8YB57 and B8YB58 and B8YB59 and B8YB60 and B8YB61 and B8YB65 and B8YB62 and B8YB63 and B8YB64)
Evolution of complex RNA polymerases: the complete archaeal RNA polymerase structure
PLoS Biol.
7
e100010
2009
Saccharolobus shibatae (B8YB53 and B8YB55 and B8YB54 and B8YB56 and B8YB57 and B8YB58 and B8YB59 and B8YB60 and B8YB61 and B8YB65 and B8YB62 and B8YB63 and B8YB64), Saccharolobus shibatae, Saccharolobus shibatae B12 (B8YB53 and B8YB55 and B8YB54 and B8YB56 and B8YB57 and B8YB58 and B8YB59 and B8YB60 and B8YB61 and B8YB65 and B8YB62 and B8YB63 and B8YB64)