Information on EC 2.7.7.5 - sulfate adenylyltransferase (ADP)

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The expected taxonomic range for this enzyme is: Archaea, Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.7.7.5
-
RECOMMENDED NAME
GeneOntology No.
sulfate adenylyltransferase (ADP)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ADP + sulfate = phosphate + adenylyl sulfate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
sulfite oxidation II
-
-
sulfate reduction
-
-
Sulfur metabolism
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
ADP:sulfate adenylyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9027-70-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
thermophilic, DSM 3772
-
-
Manually annotated by BRENDA team
Saccharomyces fragilis
-
-
-
Manually annotated by BRENDA team
-
Q9LA72
SwissProt
Manually annotated by BRENDA team
-
Q9LA72
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + phosphate
phosphate + ADP
show the reaction diagram
CDP + phosphate
phosphate + CDP
show the reaction diagram
Saccharomyces fragilis
-
-
-
?
GDP + phosphate
phosphate + GDP
show the reaction diagram
Saccharomyces fragilis
-
-
-
?
IDP + phosphate
phosphate + IDP
show the reaction diagram
Saccharomyces fragilis
-
-
-
?
phosphate + adenosine 5'-phosphoamidate
ADP + NH4+
show the reaction diagram
22% reaction velocity compared to adenosine 5'-phosphosulfate
-
-
ir
phosphate + adenosine 5'-phosphofluoride
ADP + fluoride
show the reaction diagram
8% reaction velocity compared to adenosine 5'-phosphosulfate
-
-
?
phosphate + adenosine 5'-phosphosulfate
ADP + SO42-
show the reaction diagram
phosphate + diadenosine tetraphosphate
?
show the reaction diagram
2% reaction velocity compared to adenosine 5'-phosphosulfate
-
-
?
phosphate + diadenosine triphosphate
?
show the reaction diagram
phosphate + inosine 5'-phosphosulfate
IDP + SO42-
show the reaction diagram
UDP + phosphate
phosphate + UDP
show the reaction diagram
Saccharomyces fragilis
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phosphate + adenosine 5'-phosphosulfate
ADP + SO42-
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
Saccharomyces fragilis
-
slight inhibition
Ca2+
Saccharomyces fragilis
-
strong inhibition
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenosine 5'-phosphosulfate
-
substrate inhibition above 1 mM
AMP
Saccharomyces fragilis
-
-
arsenate
chromate
Saccharomyces fragilis
-
-
diacetyl
Q9LA72;
slow but complete inactivation
diethyldicarbonate
Q9LA72;
-
F-
Saccharomyces fragilis
-
-
N-ethylmaleimide
NaCl
Saccharomyces fragilis
-
slight inhibition
nitrate
Saccharomyces fragilis
-
-
p-chloromercuribenzoate
Phenylglyoxal
Q9LA72;
incomplete inactivation
phosphate
selenate
Saccharomyces fragilis
-
-
sulfate
vanadate
Saccharomyces fragilis
-
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
Saccharomyces fragilis
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.25 - 26
adenosine 5'-phosphosulfate
0.0005
ADP
Saccharomyces fragilis
-
30°C
0.0003
GDP
Saccharomyces fragilis
-
30°C
0.0015 - 600
phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.6 - 6
adenosine 5'-phosphosulfate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11.7
phosphate
-
30°C, pH 8
167
sulfate
-
4 mM adenosine 5'-phosphosulfate, 30°C, pH 8
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.03
-
purified enzyme, 30°C, pH 7.5
0.043
-
cytoplasm, pH 8, 80°C
0.8
-
purified enzyme, 30°C, pH 8
16
Saccharomyces fragilis
-
purified enzyme, 30°C
2100
Q9LA72;
purified enzyme, pH 7.6
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
Saccharomyces fragilis
-
-
8.5 - 9
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.2 - 6.8
Hint4 degrades adenosine 5'-phosphosulfate phosphorolytically twice as fast at pH 4.2 than at pH 6.8
5.5 - 7.5
Saccharomyces fragilis
-
rapid loss of activity above pH 7.5 with UDP
5.5 - 8.5
Saccharomyces fragilis
-
with ADP, CDP and GDP
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
Saccharomyces fragilis
-
-
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
UNIPROT
ORGANISM
Treponema pallidum (strain Nichols);
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41000
Q9LA72;
SDS-PAGE
41380
Q9LA72;
MALDI-MS
41390
Q9LA72;
calculated from DNA sequence
82000
Q9LA72;
gel filtration
120000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
rapid loss of activity below pH 6 or above pH 8
643454
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
Saccharomyces fragilis
-
84% loss of activity within 1 min
60
-
69% loss of activity within 5 min
70
-
no activity after 5 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
purified enzyme stabilized by 5 mg/ml bovine serum albumin
Q9LA72;
very heat labile
-
very labile , 50% loss of activity by four times freeze and thaw
-
very labile in crude extract, partially stabilized by addition of EDTA and 2-mercaptoethanol
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18°C stable for several weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE