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Information on EC 2.7.7.49 - RNA-directed DNA polymerase and Organism(s) Tetrahymena thermophila and UniProt Accession O77448

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IUBMB Comments
Catalyses RNA-template-directed extension of the 3'- end of a DNA strand by one deoxynucleotide at a time. Cannot initiate a chain de novo. Requires an RNA or DNA primer. DNA can also serve as template. See also EC 2.7.7.7 DNA-directed DNA polymerase.
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This record set is specific for:
Tetrahymena thermophila
UNIPROT: O77448
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The taxonomic range for the selected organisms is: Tetrahymena thermophila
The enzyme appears in selected viruses and cellular organisms
Synonyms
telomerase, reverse-transcriptase, telomerase reverse transcriptase, nucleoside reverse transcriptase, hiv-1 rt, hiv-1 reverse transcriptase, gag-pol, hiv reverse transcriptase, rna-dependent dna polymerase, telomerase catalytic subunit, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
DNA nucleotidyltransferase (RNA-directed)
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MX162-RT
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MX65-RT
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nucleotidyltransferase, deoxyribonucleate, RNA-dependent
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P72
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reverse transcriptase
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revertase
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RNA revertase
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RNA-dependent DNA polymerase
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RNA-instructed DNA polymerase
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RT
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telomerase catalytic subunit
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telomerase reverse transcriptase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
deoxynucleoside-triphosphate:DNA deoxynucleotidyltransferase (RNA-directed)
Catalyses RNA-template-directed extension of the 3'- end of a DNA strand by one deoxynucleotide at a time. Cannot initiate a chain de novo. Requires an RNA or DNA primer. DNA can also serve as template. See also EC 2.7.7.7 DNA-directed DNA polymerase.
CAS REGISTRY NUMBER
COMMENTARY hide
9068-38-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the CP2 motif is in close proximity to a conserved region known as the template boundary element and acts as a RNA binding determinant. The CP2 motif binds the template boundary element to strictly define which telomerase RNA nucleotides can be reverse transcribed
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
1 * 40000, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C232A
mutation in CP2 motif, modest defect in RNA binding
F230A
mutation in CP2 motif, no effect on RNA binding
H234A
mutation in CP2 motif, modest defect in RNA binding
I229A
mutation in CP2 motif, modest defect in RNA binding
R226A
mutation in CP2 motif, strong defect in RNA binding
R237A
mutation in CP2 motif, strong defect in RNA binding
Y231A
mutation in CP2 motif, no effect on RNA binding
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Akiyama, B.M.; Gomez, A.; Stone, M.D.
A conserved motif in Tetrahymena thermophila telomerase reverse transcriptase is proximal to the RNA template and is essential for boundary definition
J. Biol. Chem.
288
22141-22149
2013
Tetrahymena thermophila (O77448), Tetrahymena thermophila
Manually annotated by BRENDA team