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Information on EC 2.7.7.43 - N-acylneuraminate cytidylyltransferase and Organism(s) Oncorhynchus mykiss and UniProt Accession Q90WG6

for references in articles please use BRENDA:EC2.7.7.43
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EC Tree
IUBMB Comments
Acts on N-acetyl- and N-glycolyl- derivatives.
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This record set is specific for:
Oncorhynchus mykiss
UNIPROT: Q90WG6
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Word Map
The taxonomic range for the selected organisms is: Oncorhynchus mykiss
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
cmp-sialic acid synthetase, cmp-neu5ac synthetase, cmp-neuac synthetase, cmp-neunac synthetase, cmp-n-acetylneuraminic acid synthetase, nmcss, cmp-sia synthetase, dmcss, cmp-sia-syn, dmcsas, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CMP-sialic acid synthetase
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acylneuraminate cytidyltransferase
-
-
-
-
CMP sialate pyrophosphorylase
-
-
-
-
CMP-N-acetylneuraminate synthase
-
-
-
-
CMP-N-acetylneuraminate synthetase
-
-
-
-
CMP-N-acetylneuraminic acid synthase
-
-
-
-
CMP-N-acetylneuraminic acid synthetase
-
-
-
-
CMP-NANA synthetase
-
-
-
-
CMP-Neu5Ac synthetase
-
-
-
-
CMP-NeuAc synthetase
-
-
-
-
CMP-NeuNAc synthetase
-
-
-
-
CMP-Sia synthetase
CMP-sialate synthase
-
-
-
-
CMP-sialate synthetase
-
-
-
-
CMP-sialic acid synthetase
-
-
-
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CMP-sialic synthetase
-
-
-
-
CMPsialate pyrophosphorylase
-
-
-
-
CMPsialate synthase
-
-
-
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cytidine 5'-monophospho-N-acetylneuraminic acid synthetase
-
-
-
-
cytidine 5'-monophosphosialic acid synthetase
-
-
-
-
cytidine 5-monophosphate N-acetylneuraminic acid synthetase
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-
-
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cytidine monophosphate sialic acid synthetase
-
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cytidine monophosphate-N-acetylneuraminic acid synthetase
-
-
-
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cytidine monophospho-sialic acid synthetase
-
-
-
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cytidine monophosphoacetylneuraminic synthetase
-
-
-
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cytidine monophosphosialate pyrophosphorylase
-
-
-
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cytidine monophosphosialate synthetase
-
-
-
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cytidyltransferase, acylneuraminate
-
-
-
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cytidylyltransferase, acetylneuraminate
-
-
-
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sialate cytidylyltransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
CTP:N-acylneuraminate cytidylyltransferase
Acts on N-acetyl- and N-glycolyl- derivatives.
CAS REGISTRY NUMBER
COMMENTARY hide
9067-82-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CTP + 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid
diphosphate + CMP-2-keto-3-deoxy-D-glycero-D-galacto-nononic acid
show the reaction diagram
enzyme shows high activity towards 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid and towards N-acetylneuraminate
-
-
?
CTP + 3-deoxy-D-glycero-D-galacto-nononate
diphosphate + CMP-3-deoxy-D-glycero-D-galacto-nononate
show the reaction diagram
-
reaction of EC 2.7.7.92
-
?
CTP + N-acetylneuraminic acid
diphosphate + CMP-N-acetylneuraminic acid
show the reaction diagram
-
-
-
?
CTP + N-acylneuraminate
diphosphate + CMP-N-acylneuraminate
show the reaction diagram
CTP + deaminoneuraminic acid
?
show the reaction diagram
-
-
-
-
?
CTP + N-acylneuraminate
diphosphate + CMP-N-acylneuraminate
show the reaction diagram
CTP + N-glycolylneuraminate
diphosphate + CMP-N-glycolylneuraminate
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
no activity with N-acetyl-9-O-acetylneuraminate
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2'-deoxy-CTP
CDP
-
0.3 mM, 45% inhibition
CMP
-
0.3 mM, 57% inhibition
Cu2+
-
1 mM, 21% inhibition
Hg2+
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1 mM, 33% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3
2-keto-3-deoxy-D-glycero-D-galacto-nononic acid
pH 9.0, 37°C
3
3-deoxy-D-glycero-D-galacto-nononate
pH 9.0, 25°C
2.8
N-acetylneuraminate
pH 9.0, 37°C
2.8
N-acetylneuraminic acid
pH 9.0, 25°C
1.7
CTP
-
pH 9.3, 30°C
2.1
N-acetylneuraminate
-
pH 9.3, 30°C
2.9
N-glycolylneuraminate
-
pH 9.3, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
NEUA_ONCMY
432
0
48242
Swiss-Prot
other Location (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
69000
160000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 69000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
unstable even at
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli and CHO cells
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Schmelter, T.; Ivanov, S.; Wember, M.; Stangier, P.; Thiem, J.; Schauer, R.
Partial purification and characterization of cytidine-5-monophosphosialate synthase from rainbow trout liver
Biol. Chem. Hoppe-Seyler
374
337-342
1993
Oncorhynchus mykiss
Manually annotated by BRENDA team
Nakata, D.; Munster, A.K.; Gerardy-Schahn, R.; Aoki, N.; Matsuda, T.; Kitajima, K.
Molecular cloning of a unique CMP-sialic acid synthetase that effectively utilizes both deaminoneuraminic acid (KDN) and N-acetylneuraminic acid (Neu5Ac) as substrates
Glycobiology
11
685-692
2001
Oncorhynchus mykiss (Q90WG6), Oncorhynchus mykiss, Mus musculus (Q99KK2), Mus musculus
Manually annotated by BRENDA team
Bravo, I.G.; Reglero, A.
The cytidylyltransferases family: properties, kinetics, genomic and phylogeny: The cytidylyltransferases family: properties, kinetics, genomic and phylogeny
Recent Res. Devel. Biochem.
4
223-254
2003
Bos taurus, Escherichia coli, Equus caballus, [Haemophilus] ducreyi, Cricetinae, Erinaceidae, Homo sapiens, Mannheimia haemolytica, Neisseria meningitidis, Oncorhynchus mykiss, Rattus norvegicus, Streptococcus agalactiae, Sus scrofa
-
Manually annotated by BRENDA team
Fujita, A.; Sato, C.; Munster-Kuhnel, A.K.; Gerardy-Schahn, R.; Kitajima, K.
Development of a simple and efficient method for assaying cytidine monophosphate sialic acid synthetase activity using an enzymatic reduced nicotinamide adenine dinucleotide/oxidized nicotinamide adenine dinucleotide converting system
Anal. Biochem.
337
12-21
2005
Mus musculus, Oncorhynchus mykiss
Manually annotated by BRENDA team
Tiralongo, J.; Fujita, A.; Sato, C.; Kitajima, K.; Lehmann, F.; Oschlies, M.; Gerardy-Schahn, R.; Muenster-Kuehnel, A.K.
The rainbow trout CMP-sialic acid synthetase utilises a nuclear localization signal different from that identified in the mouse enzyme
Glycobiology
17
945-954
2007
Oncorhynchus mykiss (Q90WG6), Oncorhynchus mykiss
Manually annotated by BRENDA team