Any feedback?
Information on EC 2.7.7.41 - phosphatidate cytidylyltransferase and Organism(s) Homo sapiens and UniProt Accession Q92903
for references in articles please use BRENDA:EC2.7.7.41Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
2.7.7.41 phosphatidate cytidylyltransferaseSpecify your search results
Word Map
- 2.7.7.41
- phospholipid
- phosphatidylinositol
- phosphatidylglycerol
- cardiolipin
- cds1
- liponucleotide
-
raetz
-
phosphatidyltransferase
-
diphosphate-diacylglycerol
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
cdp-diacylglycerol synthase, cdp-dg, phosphatidate cytidylyltransferase, tam41, cdp-dag synthase, tamm41, ctp:phosphatidate cytidylyltransferase, cdp-diglyceride synthetase, cdp-dg synthase, tbcds, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
CDS1
CDP-DAG synthase
CDP-DG
-
-
-
-
CDP-DG synthetase
-
-
-
-
CDP-diacylglyceride synthetase
-
-
-
-
CDP-diacylglycerol synthase
CDP-diglyceride pyrophosphorylase
-
-
-
-
CDP-diglyceride synthetase
-
-
-
-
CDPdiglyceride pyrophosphorylase
-
-
-
-
CDS
CDS2
CTP-diacylglycerol synthetase
-
-
-
-
CTP:1,2-diacylglycerophosphate-cytidyl transferase
-
-
-
-
CTP:phosphatidate cytidylyltransferase
-
-
-
-
cytidine diphosphoglyceride pyrophosphorylase
-
-
-
-
cytidylyltransferase, phosphatidate
-
-
-
-
DAG synthetase
-
-
-
-
phosphatidate cytidyltransferase
-
-
-
-
phosphatidic acid cytidylyltransferase
-
-
-
-
CDP-DAG synthase
-
-
-
-
CDP-diacylglycerol synthase
-
-
-
-
CDS
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
nucleotidyl group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
CTP:phosphatidate cytidylyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY
9067-83-8
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
CTP + 1,2-diarachidonoyl-sn-phosphatidic acid
diphosphate + CDP-1,2-diarachidonoylglycerol
-
-
-
?
CTP + 1,2-dilinoleoyl-sn-phosphatidic acid
diphosphate + CDP-1,2-dilinoleoylglycerol
-
-
-
?
CTP + 1,2-dioleoyl-sn-phosphatidic acid
diphosphate + CDP-1,2-dioleoylglycerol
-
-
-
?
CTP + 1,2-diolinoleoyl-sn-phosphatidic acid
diphosphate + CDP-1,2-diolinoleoylglycerol
-
-
-
?
CTP + 1-palmitoyl-2-arachidonoyl-sn-phosphatidic acid
diphosphate + CDP-1-palmitoyl-2-arachidonoylglycerol
-
-
-
?
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
diphosphate + CDP-1-stearoyl-2-arachidonoyl-glycerol
-
-
-
?
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
diphosphate + CDP-1-stearoyl-2-arachidonoylglycerol
-
-
-
?
CTP + 1-stearoyl-2-docosahexaenoyl-sn-phosphatidic acid
diphosphate + CDP-1-stearoyl-2-docosahexaenoylglycerol
-
-
-
?
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
diphosphate + CDP-1-stearoyl-2-linoleoyl-glycerol
-
-
-
?
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
diphosphate + CDP-1-stearoyl-2-linoleoylglycerol
-
-
-
?
CTP + 1-stearoyl-2-oleoyl-sn-phosphatidic acid
diphosphate + CDP-1-stearoyl-2-oleoylglycerol
-
-
-
?
CTP + 1,2-diarachidonoyl-sn-phosphatidic acid
diphosphate + CDP-1,2-diarachidonoylglycerol
50% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
-
?
CTP + 1,2-dilinoleoyl-sn-phosphatidic acid
diphosphate + CDP-1,2-dilinoleoylglycerol
20% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
-
?
CTP + 1,2-dioleoyl-sn-phosphatidic acid
diphosphate + CDP-1,2-dioleoylglycerol
less than 5% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
-
?
CTP + 1,2-diolinoleoyl-sn-phosphatidic acid
diphosphate + CDP-1,2-diolinoleoylglycerol
-
-
-
?
CTP + 1-palmitoyl-2-arachidonoyl-sn-phosphatidic acid
diphosphate + CDP-1-palmitoyl-2-arachidonoylglycerol
65% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
-
?
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
diphosphate + CDP-1-stearoyl-2-arachidonoyl-glycerol
-
-
-
?
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
diphosphate + CDP-1-stearoyl-2-arachidonoylglycerol
-
-
-
?
CTP + 1-stearoyl-2-docosahexaenoyl-sn-phosphatidic acid
diphosphate + CDP-1-stearoyl-2-docosahexaenoylglycerol
-
-
-
?
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
diphosphate + CDP-1-stearoyl-2-linoleoyl-glycerol
-
-
-
?
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
diphosphate + CDP-1-stearoyl-2-linoleoylglycerol
20% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
-
?
CTP + 1-stearoyl-2-oleoyl-sn-phosphatidic acid
diphosphate + CDP-1-stearoyl-2-oleoylglycerol
20% of the activity with 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
-
?
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
isozyme CDS1 shows no particular substrate specificity, displaying similar activities for almost all substrates tested
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
the level of CDP-diacylglycerol synthetase 1 is not a critical determinant of cellular phosphatidylinositol content. This argues against a determining role of the activity in the regulation of phosphatidylinositol biosynthesis
-
-
?
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
isozyme CDS2 is selective for the acyl chains at the sn-1 and sn-2 positions, the most preferred species being 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
-
mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme
-
-
?
dCTP + phosphatidate
diphosphate + dCDPdiacylglycerol
-
mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme
-
-
?
additional information
?
-
isoform CDS1 appears to have no substrate preference for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, with variations of the sn-1 and sn-2 acyl chains resulting in no significant changes in preference
-
-
?
additional information
?
-
isoform CDS1 appears to have no substrate preference for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, with variations of the sn-1 and sn-2 acyl chains resulting in no significant changes in preference
-
-
?
additional information
?
-
-
isoform CDS1 appears to have no substrate preference for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, with variations of the sn-1 and sn-2 acyl chains resulting in no significant changes in preference
-
-
?
additional information
?
-
mixed micelle-based enzymatic activity with recombinant enzyme. Substrate specificity of CDS1, overview
-
-
?
additional information
?
-
mixed micelle-based enzymatic activity with recombinant enzyme. Substrate specificity of CDS1, overview
-
-
?
additional information
?
-
-
mixed micelle-based enzymatic activity with recombinant enzyme. Substrate specificity of CDS1, overview
-
-
?
additional information
?
-
CDS2 shows substrate specificity at both the sn-1 and sn-2 acyl chain positions. The most preferred substrate is 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
-
?
additional information
?
-
CDS2 shows substrate specificity at both the sn-1 and sn-2 acyl chain positions. The most preferred substrate is 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
-
?
additional information
?
-
-
CDS2 shows substrate specificity at both the sn-1 and sn-2 acyl chain positions. The most preferred substrate is 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
-
?
additional information
?
-
mixed micelle-based enzymatic activity with recombinant enzyme. Substrate specificity of CDS2, overview
-
-
?
additional information
?
-
mixed micelle-based enzymatic activity with recombinant enzyme. Substrate specificity of CDS2, overview
-
-
?
additional information
?
-
-
mixed micelle-based enzymatic activity with recombinant enzyme. Substrate specificity of CDS2, overview
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
diphosphate + CDP-1-stearoyl-2-arachidonoyl-glycerol
-
-
-
?
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
diphosphate + CDP-1-stearoyl-2-linoleoyl-glycerol
-
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
the level of CDP-diacylglycerol synthetase 1 is not a critical determinant of cellular phosphatidylinositol content. This argues against a determining role of the activity in the regulation of phosphatidylinositol biosynthesis
-
-
?
CTP + 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
diphosphate + CDP-1-stearoyl-2-arachidonoyl-glycerol
-
-
-
?
CTP + 1-stearoyl-2-linoleoyl-sn-phosphatidic acid
diphosphate + CDP-1-stearoyl-2-linoleoyl-glycerol
-
-
-
?
CTP + phosphatidate
diphosphate + CDPdiacylglycerol
-
mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme
-
-
?
dCTP + phosphatidate
diphosphate + dCDPdiacylglycerol
-
mammalian enzymes show similar efficacy for CTP and dCTP, however CTP is the preferred substrate in vivo, since dCDP-diacylglycerol is not detectable in mammalian tissues. In Escherichia coli equivalent amounts of CDP-diacylglycerol and dCDP-diacylglycerol are detected. Arabinofuranosylcytosine is also found to be incorporated into lipid in mammalian cells, suggesting that it is a substrate for the enzyme
-
-
?
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
isozyme CDS1 shows no particular substrate specificity, displaying similar activities for almost all substrates tested
-
-
?
CTP + phosphatidate
diphosphate + CDP-diacylglycerol
isozyme CDS2 is selective for the acyl chains at the sn-1 and sn-2 positions, the most preferred species being 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
L-alpha-phosphatidylinositol 4,5-bisphosphate
strongest inhibitor among the phosphatidylinositols tested
-
phosphatidylinositol
CDS1 shows no acyl chain-dependent inhibition
phosphatidylinositol-(4,5)-bisphosphate
CDS1 is inhibited by their anionic phospholipid end products, with phosphatidylinositol-(4,5)-bisphosphate showing the strongest inhibition
L-alpha-phosphatidylinositol 4,5-bisphosphate
1 mol%, 80% inhibition
-
phosphatidylinositol-(4,5)-bisphosphate
80% inhibition at 1 mol%, CDS2 is inhibited by its anionic phospholipid end products, with phosphatidylinositol-(4,5)-bisphosphate showing the strongest inhibition
phosphatidylinositol
significant inhibition of isoform CDS2 by both soybean and liver PI, with 15?20% residual activity
phosphatidylinositol
15-20% inhibition by soybean and liver phosphatidylinositol, inhibition of CDS2 by phosphatidylinositol is acyl chain-dependent, with the strongest inhibition seen with the 1-stearoyl-2-arachidonoyl species
additional information
no significant inhibition in activity when either soybean or liver phosphatidylinositol is used at equimolar concentrations with respect to substrate. Neither soy nor egg phosphatidylglycerol has any significant inhibitory effect when used at equimolar concentrations with respect to substrate. Isoform CDS1 shows no acyl chain-dependent inhibition for 1-stearoyl-2-arachidonoyl-snphosphatidylinositol, 1-stearoyl-2-linoleoyl-sn-phosphatidylinositol, or 1,2-diolinoleoyl-sn-phosphatidylinositol
-
additional information
no significant inhibition in activity when either soybean or liver phosphatidylinositol is used at equimolar concentrations with respect to substrate. Neither soy nor egg phosphatidylglycerol has any significant inhibitory effect when used at equimolar concentrations with respect to substrate. Isoform CDS1 shows no acyl chain-dependent inhibition for 1-stearoyl-2-arachidonoyl-snphosphatidylinositol, 1-stearoyl-2-linoleoyl-sn-phosphatidylinositol, or 1,2-diolinoleoyl-sn-phosphatidylinositol
-
additional information
-
no significant inhibition in activity when either soybean or liver phosphatidylinositol is used at equimolar concentrations with respect to substrate. Neither soy nor egg phosphatidylglycerol has any significant inhibitory effect when used at equimolar concentrations with respect to substrate. Isoform CDS1 shows no acyl chain-dependent inhibition for 1-stearoyl-2-arachidonoyl-snphosphatidylinositol, 1-stearoyl-2-linoleoyl-sn-phosphatidylinositol, or 1,2-diolinoleoyl-sn-phosphatidylinositol
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
Km value 0.8 mol%, pH 8.0, 22°C
-
additional information
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
Km value 0.8 mol%, pH 8.0, 22°C
-
additional information
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
Km value 0.8 mol%, pH 8.0, 22°C
-
additional information
1-stearoyl-2-linoleoyl-sn-phosphatidic acid
KM value 0.6 mol%, pH 8.0, 22°C
-
additional information
1-stearoyl-2-linoleoyl-sn-phosphatidic acid
KM value 0.6 mol%, pH 8.0, 22°C
-
additional information
1-stearoyl-2-linoleoyl-sn-phosphatidic acid
-
KM value 0.6 mol%, pH 8.0, 22°C
-
additional information
additional information
0.8 mol% for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, 0.6 mol% for 1-stearoyl-2-linoleoyl-sn-phosphatidic acid, kinetics, overview
-
additional information
additional information
0.8 mol% for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, 0.6 mol% for 1-stearoyl-2-linoleoyl-sn-phosphatidic acid, kinetics, overview
-
additional information
additional information
-
0.8 mol% for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, 0.6 mol% for 1-stearoyl-2-linoleoyl-sn-phosphatidic acid, kinetics, overview
-
additional information
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
Km value 1.4 mol%, pH 8.0, 22°C
-
additional information
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
Km value 1.4 mol%, pH 8.0, 22°C
-
additional information
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
-
Km value 1.4 mol%, pH 8.0, 22°C
-
additional information
1-stearoyl-2-linoleoyl-sn-phosphatidic acid
KM value 0.9 mol%, pH 8.0, 22°C
-
additional information
1-stearoyl-2-linoleoyl-sn-phosphatidic acid
KM value 0.9 mol%, pH 8.0, 22°C
-
additional information
1-stearoyl-2-linoleoyl-sn-phosphatidic acid
-
KM value 0.9 mol%, pH 8.0, 22°C
-
additional information
additional information
1.4 mol% for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, 0.9 mol% for 1-stearoyl-2-linoleoyl-sn-phosphatidic acid, kinetics, overview
-
additional information
additional information
1.4 mol% for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, 0.9 mol% for 1-stearoyl-2-linoleoyl-sn-phosphatidic acid, kinetics, overview
-
additional information
additional information
-
1.4 mol% for 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, 0.9 mol% for 1-stearoyl-2-linoleoyl-sn-phosphatidic acid, kinetics, overview
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2.9
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
pH 8.0, 22°C, recombinant CDS1
-
3.2
1-stearoyl-2-linoleoyl-sn-phosphatidic acid
pH 8.0, 22°C, recombinant CDS1
-
8
1-stearoyl-2-arachidonoyl-sn-phosphatidic acid
pH 8.0, 22°C, recombinant CDS2
-
2.9
1-stearoyl-2-linoleoyl-sn-phosphatidic acid
pH 8.0, 22°C, recombinant CDS2
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
2.4
substrate 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, pH 8.0, 22°C
4.5
substrate 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid, pH 8.0, 22°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GFP-tagged CDS1 shows a typical ER tubular localization when it is expressed in COS7 cells
-
cofractionation of enzyme and phosphatidylinositol synthase activities with cholesterol-poor, endoplasmic reticulum membranes. Enzymic activity localizes to endoplasmic reticulum membrane fragments containing calnexin and type III inositol (1,4,5)-trisphosphate receptors. CDP-diacylglycerol and phposphatidylinositol syntheses are restricted to a subfraction of the calnexin-positive endoplasmic reticulum
GFP-tagged CDS2 shows a typical ER tubular localization when it is expressed in COS7 cells
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
knocking down CDS1 results in the formation of giant or supersized lipid droplets in cultured cells. The levels of many phosphatidate species are significantly increased upon knocking down CDS1, the amount of phosphatidate in the endoplasmic reticulum is dramatically increased upon knocking down CDS1, overview. The changes in phosphatidate level and localization may underlie the formation of giant lipid droplets as well as the block in adipogenesis in CDS-deficient cells
metabolism
physiological function
malfunction
metabolism
physiological function
metabolism
the two isoforms of human CDS, CDS1 and CDS2, show different acyl chain specificities for its lipid substrate. CDS1 and CDS2 can create different CDP-DAG pools that may serve to enrich different phospholipid species with specific acyl chains
metabolism
the enzyme is critical for maintaining phosphoinositide levels during phospholipase C signaling
physiological function
isoforms CDS1 and CDS2 could create different CDP-diacylglycerol pools that may serve to enrich different phospholipid species with specific acyl chains. CDS1 shows no particular substrate specificity, displaying similar activities for almost all substrates tested, and shows no acyl chain-dependent inhibition. Both isoforms CDS1 and CDS2 are inhibited by their anionic phospholipid end products
physiological function
CDP-diacylglycerol synthases (CDS) are critical enzymes that catalyze the formation of CDP-diacylglycerol (CDP-DAG) from phosphatidic acid
physiological function
CDP-diacylglycerol synthases regulate the growth of lipid droplets and adipocyte development, role of CDP-diacylglycerol in lipid storage in mammals. The expansion of lipid droplets and the differentiation of preadipocytes are two important aspects of mammalian lipid storage, CDS1 and CDS2 are important regulators of lipid storage
malfunction
knocking down CDS2 results in the formation of giant or supersized lipid droplets in cultured cells. Only a small number of phosphatidate species are increased upon depleting CDS2, the amount of phosphatidate in the endoplasmic reticulum is dramatically increased upon knocking down CDS2, overview. The changes in phosphatidate level and localization may underlie the formation of giant lipid droplets as well as the block in adipogenesis in CDS-deficient cells
malfunction
isoform CDS2 deficiency, but not isoform CDS1 deficiency, promotes the lipid droplet association of diacylglycerol-O-acyltransferase 2 and glycerol-3-phosphateacyltransferase 4 and impairs initial lipid droplet maturation
metabolism
the two isoforms of human CDS, CDS1 and CDS2, show different acyl chain specificities for its lipid substrate. CDS1 and CDS2 can create different CDP-DAG pools that may serve to enrich different phospholipid species with specific acyl chains
metabolism
the enzyme is critical for maintaining phosphoinositide levels during phospholipase C signaling
physiological function
isoforms CDS1 and CDS2 could create different CDP-diacylglycerol pools that may serve to enrich different phospholipid species with specific acyl chains. CDS2 is selective for the acyl chains at the sn-1 and sn-2 positions, the most preferred species being 1-stearoyl-2-arachidonoyl-sn-phosphatidic acid. Inhibition of CDS2 by phosphatidylinositol is also acyl chain-dependent, with the strongest inhibition seen with the 1-stearoyl-2-arachidonoyl species. Both isoforms CDS1 and CDS2 are inhibited by their anionic phospholipid end products
physiological function
CDP-diacylglycerol synthases (CDS) are critical enzymes that catalyze the formation of CDP-diacylglycerol (CDP-DAG) from phosphatidic acid
physiological function
CDP-diacylglycerol synthases regulate the growth of lipid droplets and adipocyte development, role of CDP-diacylglycerol in lipid storage in mammals. The expansion of lipid droplets and the differentiation of preadipocytes are two important aspects of mammalian lipid storage, CDS1 and CDS2 are important regulators of lipid storage
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). CDS1_HUMAN
461
7
53304
Swiss-Prot
Mitochondrion (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
transient siRNA knockdown of CDS1 in HeLa cells downregulates CDS1 by 81%. In siCDS2 HeLa cells, the mRNA expression of CDS1 is increased by 2.6fold, but in siCDS1 cells, the CDS2 level is unchanged. Knocking down CDS1 results in the formation of giant or supersized lipid droplets in cultured cells
additional information
transient siRNA knockdown of CDS1 in HeLa cells downregulates CDS1 by 81%. In siCDS2 HeLa cells, the mRNA expression of CDS1 is increased by 2.6fold, but in siCDS1 cells, the CDS2 level is unchanged. Knocking down CDS1 results in the formation of giant or supersized lipid droplets in cultured cells
additional information
transient siRNA knockdown of CDS1 in HeLa cells downregulates CDS2 by 90%. In siCDS2 HeLa cells, the mRNA expression of CDS1 is increased by 2.6fold, but in siCDS1 cells, the CDS2 level is unchanged. Knocking down CDS2 results in the formation of giant or supersized lipid droplets in cultured cells
additional information
transient siRNA knockdown of CDS1 in HeLa cells downregulates CDS2 by 90%. In siCDS2 HeLa cells, the mRNA expression of CDS1 is increased by 2.6fold, but in siCDS1 cells, the CDS2 level is unchanged. Knocking down CDS2 results in the formation of giant or supersized lipid droplets in cultured cells
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant expression of C-terminally myc-tagged and GFP-tagged CDS1 in COS7 cells
expression under the control of a GAL1 promoter in a null cds1 mutant yeast strain complements its growth defect and produces enzyme activity when induced with galactose
-
recombinant expression of C-terminally myc-tagged and GFP-tagged CDS2 in COS7 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Weeks, R.; Dowhan, W.; Shen, H.; Balantac, N.; Meengs, B.; Nudelman, E.; Leung, D.W.
Isolation and expression of an isoform of human CDP-diacylglycerol synthase cDNA
DNA Cell Biol.
16
281-289
1997
Homo sapiens
Heacock, A.M.; Agranoff, B.W.
CDP-diacylglycerol synthase from mammalian tissues
Biochim. Biophys. Acta
1348
166-172
1997
Cavia porcellus, Drosophila sp. (in: flies), Escherichia coli, Homo sapiens, Rattus norvegicus, Sus scrofa
Lykidis, A.; Jackson, P.; Rock, C.O.; Jackowski, S.
The role of CDP-diacylglycerol synthetase and phosphatidylinositol synthase activity levels in the regulation of cellular phosphatidylinositol content
J. Biol. Chem.
272
33402-33409
1997
Homo sapiens (Q92903), Homo sapiens
Waugh, M.G.; Minogue, S.; Clayton, E.L.; Hsuan, J.J.
CDP-diacylglycerol phospholipid synthesis in detergent-soluble, non-raft, membrane microdomains of the endoplasmic reticulum
J. Lipid Res.
52
2148-2158
2011
Homo sapiens
DSouza, K.; Kim, Y.J.; Balla, T.; Epand, R.M.
Distinct properties of the two isoforms of CDP-diacylglycerol synthase
Biochemistry
53
7358-7367
2014
Homo sapiens (O95674), Homo sapiens (Q92903), Homo sapiens
Qi, Y.; Kapterian, T.S.; Du, X.; Ma, Q.; Fei, W.; Zhang, Y.; Huang, X.; Dawes, I.W.; Yang, H.
CDP-diacylglycerol synthases regulate the growth of lipid droplets and adipocyte development
J. Lipid Res.
57
767-780
2016
Homo sapiens (O95674), Homo sapiens (Q92903), Mus musculus (P98191), Mus musculus (Q99L43)
Blunsom, N.J.; Cockcroft, S.
CDP-diacylglycerol synthases (CDS) gateway to phosphatidylinositol and cardiolipin synthesis
Front. Cell Dev. Biol.
8
63
2020
Aeropyrum pernix, Saccharomyces cerevisiae, Drosophila melanogaster, Schizosaccharomyces pombe, Streptococcus mitis, Streptococcus oralis, Thermotoga maritima, Toxoplasma gondii, Trypanosoma brucei brucei, Eimeria falciformis, Arabidopsis thaliana (O04928), Arabidopsis thaliana (O49639), Arabidopsis thaliana (Q1PE48), Arabidopsis thaliana (Q94A03), Arabidopsis thaliana (Q9M001), Escherichia coli (P0ABG1), Danio rerio (Q3B7H2), Homo sapiens (Q92903), Homo sapiens (Q95674)
EXTERNAL LINKS (specific for EC-Number 2.7.7.41)
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
