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EC Tree
IUBMB Comments Involved in the biosynthesis of teichoic acid linkage units in bacterial cell walls.
The taxonomic range for the selected organisms is: Bacillus subtilis The enzyme appears in selected viruses and cellular organisms
Synonyms
glycerol-3-phosphate cytidylyltransferase, ctp:glycerol-3-phosphate cytidylyltransferase, cdp-glycerol pyrophosphorylase, glycerol 3-phosphate cytidylyltransferase, gro-pct, aq185, aq1368, glycerol phosphate cytidylyltransferase, ctp:glycerol 3-phosphate cytidylyltransferase,
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CDP-glycerol pyrophosphorylase
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CDPglycerol pyrophosphorylase
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CTP:glycerol 3-phosphate cytidylyltransferase
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cytidine diphosphate glycerol pyrophosphorylase
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cytidine diphosphoglycerol pyrophosphorylase
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cytidylyltransferase, glycerol 3-phosphate
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CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol
CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol
mechanism
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CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol
rapid equilibrium random order mechanism
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nucleotidyl group transfer
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CTP:sn-glycerol-3-phosphate cytidylyltransferase
Involved in the biosynthesis of teichoic acid linkage units in bacterial cell walls.
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CTP + sn-glycerol 3-phosphate
diphosphate + CDPglycerol
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?
CTP + sn-glycerol 3-phosphate
diphosphate + CDPglycerol
dCTP + glycerol 3-phosphate
diphosphate + dCDPglycerol
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about 95% of the activity with CTP
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?
additional information
?
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CTP + sn-glycerol 3-phosphate
diphosphate + CDPglycerol
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?
CTP + sn-glycerol 3-phosphate
diphosphate + CDPglycerol
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?
CTP + sn-glycerol 3-phosphate
diphosphate + CDPglycerol
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enzyme of teichoic acid synthesis
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?
CTP + sn-glycerol 3-phosphate
diphosphate + CDPglycerol
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repression of synthesis occurs at the onset of phosphate starvation and is accompanied by inhibition or inactivation of CDPglycerol pyrophosphorylase
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?
CTP + sn-glycerol 3-phosphate
diphosphate + CDPglycerol
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CDPglycerol may function in: synthesis of lipids
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?
additional information
?
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no substrate: phosphoethanolamine
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?
additional information
?
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no substrate: UTP
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?
additional information
?
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no substrate: GTP
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?
additional information
?
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no substrate: ATP
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?
additional information
?
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no substrate: ribitol phosphate
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?
additional information
?
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no substrate: phosphocholine
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additional information
?
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no substrate: cytidine diphosphate ribitol
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?
additional information
?
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no substrate: ITP
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?
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CTP + sn-glycerol 3-phosphate
diphosphate + CDPglycerol
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-
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?
CTP + sn-glycerol 3-phosphate
diphosphate + CDPglycerol
CTP + sn-glycerol 3-phosphate
diphosphate + CDPglycerol
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-
-
?
CTP + sn-glycerol 3-phosphate
diphosphate + CDPglycerol
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enzyme of teichoic acid synthesis
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?
CTP + sn-glycerol 3-phosphate
diphosphate + CDPglycerol
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repression of synthesis occurs at the onset of phosphate starvation and is accompanied by inhibition or inactivation of CDPglycerol pyrophosphorylase
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?
CTP + sn-glycerol 3-phosphate
diphosphate + CDPglycerol
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CDPglycerol may function in: synthesis of lipids
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?
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Fe2+
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divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required
Co2+
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Co2+
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divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required
Mg2+
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Mg2+
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divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required
Mn2+
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Mn2+
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divalent cation: Co2+, Mg2+, Mn2+ or Fe2+ required
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0.45 - 141
glycerol 3-phosphate
0.5
CTP
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H84A mutant
0.63
CTP
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D66E mutant and D94E mutant
1.02
CTP
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D11A mutant and R113A mutant
0.45
glycerol 3-phosphate
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D66E mutant
0.53
glycerol 3-phosphate
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H84A mutant
0.79
glycerol 3-phosphate
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R63A mutant
0.85
glycerol 3-phosphate
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E39A mutant, D11E mutant and D38E mutant
0.89
glycerol 3-phosphate
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S118A mutant
1.01
glycerol 3-phosphate
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D94E mutant
1.06
glycerol 3-phosphate
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K25A mutant
1.09
glycerol 3-phosphate
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wild-type
1.19
glycerol 3-phosphate
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C106A mutant
1.26
glycerol 3-phosphate
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H14A mutant
1.3
glycerol 3-phosphate
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T114A mutant
1.37
glycerol 3-phosphate
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K22A mutant
1.46
glycerol 3-phosphate
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K103A mutant
1.56
glycerol 3-phosphate
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T119A mutant
1.72
glycerol 3-phosphate
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D87A mutant and R55A mutant
1.76
glycerol 3-phosphate
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D11A mutant
1.78
glycerol 3-phosphate
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K19A mutant
1.85
glycerol 3-phosphate
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D92A mutant
2
glycerol 3-phosphate
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E67A mutant
2.22
glycerol 3-phosphate
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H17A mutant
2.6
glycerol 3-phosphate
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R113A mutant
3.23
glycerol 3-phosphate
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pH 8, 37°C
3.93
glycerol 3-phosphate
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R113K mutant
4.34
glycerol 3-phosphate
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E115A mutant
4.85
glycerol 3-phosphate
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D66A mutant
8.12
glycerol 3-phosphate
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D38A mutant
11.4
glycerol 3-phosphate
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R55K mutant
14.2
glycerol 3-phosphate
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W74A mutant
141
glycerol 3-phosphate
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D94A mutant
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0.001
CTP
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H14A mutant
0.007
CTP
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H17A mutant and H84A mutant
0.02
CTP
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D11E mutant and D94E mutant
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Uniprot
brenda
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brenda
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brenda
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14800
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2 * 14800, gene expression in Escherichia coli, SDS-PAGE
15271
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2 * 15271, calculated from the nucleotide sequence
30500
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conditional lethal mutant 168, gel filtration
30900
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gene expression in E. coli, gel filtration
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dimer
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dimer
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2 * 14800, gene expression in Escherichia coli, SDS-PAGE
dimer
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2 * 15271, calculated from the nucleotide sequence
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complexed with CDPglycerol
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K44A
less active than wild-type enzyme
K46A
less active than wild-type enzyme
C106A
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mutant with kinetic constants similar to wild-type enzyme
D11A
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mutant with low kcat value
D11E
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mutant with low kcat value
D38A
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mutant with high Km value
D38E
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kcat similar to wild-type enzyme
D66A
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mutant with low kcat value, defective in overall structure
D66E
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mutant with low kcat value
D87A
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mutant with kinetic constants similar to wild-type enzyme
D92A
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mutant with kinetic constants similar to wild-type enzyme
D94A/H14A
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binding constants for CTP and glycerol 3-phosphate are similar to wild-type enzyme
D94E mutant
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mutant with low kcat value
E115A
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mutant with kinetic constants similar to wild-type enzyme
E39A
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mutant with kinetic constants similar to wild-type enzyme
E67A
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mutant with kinetic constants similar to wild-type enzyme
H84A
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mutant with low kcat value
K103A
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mutant with kinetic constants similar to wild-type enzyme
K19A
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mutant with kinetic constants similar to wild-type enzyme
K22A
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mutant with kinetic constants similar to wild-type enzyme
K25A
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mutant with kinetic constants similar to wild-type enzyme
R113A
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mutant with low kcat value
R113K
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mutant with low kcat value
R55A
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mutant with low kcat value
R55K
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mutant with low kcat value
R63A
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mutant with low kcat value
S118A
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mutant with low kcat value t
T114A
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mutant with low kcat value
T119A
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mutant with low kcat value
W74A
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mutant with high Km value
D94A
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mutant with high Km value
D94A
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initial binding constant is similar to wild-type enzyme
H14A
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mutant with low kcat value, dramatic loss of activity
H14A
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Kd value for binding of a single substrate is similar to those for the wild-type enzyme
H17A
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mutant with low kcat value, dramatic loss of activity
H17A
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initial binding constant is similar to wild-type enzyme
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10
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37°C, 4 h, about 10% loss of activity
645242
6.5
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37°C, 4 h, about 30% loss of activity
645242
7.5 - 9.5
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37°C, 4 h, stable
645242
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40
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not stable for longer than 30 min
additional information
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thermostability of enzyme gro-PCT in extract of strains bearing mutations in tagA, tagB and tagF genes
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-20°C, about 20% loss of activity after 1 month
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-80°C, pure enzyme stable for at least 7 months
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gene expression in E. coli
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gene expressed in Escherichia coli
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Shaw, D.R.D.
Phosphorolysis and enzymic synthesis of cytidine diphosphate glycerol and cytidine diphosphate ribitol
Biochem. J.
82
297-312
1962
Bacillus subtilis, Saccharomyces cerevisiae, Chlorella vulgaris, Escherichia coli, Lactiplantibacillus plantarum, Staphylococcus aureus, Propionibacterium freudenreichii subsp. shermanii
brenda
Cheah, S.C.; Hussey, H.; Baddiley, J.
Control of synthesis of wall teichoic acid in phosphate-starved cultures of Bacillus subtilis W23
Eur. J. Biochem.
118
497-500
1981
Bacillus subtilis, Bacillus subtilis W23
brenda
Park, Y.S.; Sweitzer, T.D.; Dixon, J.E.; Kent, C.
Expression, purification, and characterization of CTP:glycerol-3-phosphate cytidylyltransferase from Bacillus subtilis
J. Biol. Chem.
268
16648-16654
1993
Bacillus subtilis
brenda
Pooley, H.M.; Abellan, F.X.; Karamata, D.
A conditional-lethal mutant of bacillus subtilis 168 with a thermosensitive glycerol-3-phosphate cytidylyltransferase, an enzyme specific for the synthesis of the major wall teichoic acid
J. Gen. Microbiol.
137
921-928
1991
Bacillus subtilis
brenda
Park, Y.S.; Gee, P.; Sanker, S.; Schurter, E.J.; Zuiderweg, E.R.; Kent, C.
Identification of functional conserved residues of CTP:glycerol-3-phosphate cytidylyltransferase. Role of histidines in the conserved HXGH in catalysis
J. Biol. Chem.
272
15161-15166
1997
Bacillus subtilis
brenda
Pattridge, K.A.; Weber, C.H.; Friesen, J.A.; Sanker, S.; Kent, C.; Ludwig, M.L.
Glycerol-3-phosphate cytidylyltransferase: Structural changes induced by binding of CDP-glycerol and the role of lysine residues in catalysis
J. Biol. Chem.
278
51863-51871
2003
Bacillus subtilis (P27623), Bacillus subtilis
brenda
Sanker, S.; Campbell, H.A.; Kent, C.
Negative cooperativity of substrate binding but not enzyme activity in wild-type and mutant forms of CTP:glycerol-3-phosphate cytidylyltransferase
J. Biol. Chem.
276
37922-37928
2001
Bacillus subtilis
brenda