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CTP + 3-deoxy-D-manno-octulosonate
diphosphate + CMP-3-deoxy-D-manno-octulosonate
CTP + 3,5-dideoxy-5-fluoro-beta-D-manno-2-octulopyranosonic acid
diphosphate + CMP-3,5-dideoxy-5-fluoro-beta-D-manno-2-octulopyranosonic acid
-
-
-
-
r
CTP + 3-deoxy-D-manno-octulosonate
CMP-3-deoxy-D-manno-octulosonate + diphosphate
-
-
-
-
?
CTP + 3-deoxy-D-manno-octulosonate
diphosphate + CMP-3-deoxy-D-manno-octulosonate
CTP + 5-deoxy-Kdo
diphosphate + CMP-5-deoxy-3-deoxy-D-manno-octulosonate
-
-
-
-
r
CTP + 5-epi-Kdo
diphosphate + CMP-5-epi-3-deoxy-D-manno-octulosonate
-
-
-
-
r
CTP + 5-fluoro-2-keto-3,5-dideoxyoctulosonate
diphosphate + CMP-5-fluoro-2-keto-3,5-dideoxy octulosonate
-
-
-
-
r
CTP + 8-azido-3,8-dideoxy-D-manno-oct-2-ulosonate
diphosphate + CMP-azido-3,8-dideoxy-D-manno-oct-2-ulosonate
the enzyme does not efficiently recognize azido-3,8-dideoxy-D-manno-oct-2-ulosonate as a substrate
-
-
?
dCTP + 3-deoxy-D-manno-octulosonate
diphosphate + dCMP-3-deoxy-D-manno-octulosonate
UTP + 3-deoxy-D-manno-octulosonate
diphosphate + UMP-3-deoxy-D-manno-octulosonate
additional information
?
-
CTP + 3-deoxy-D-manno-octulosonate
diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
-
-
r
CTP + 3-deoxy-D-manno-octulosonate
diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
-
-
r
CTP + 3-deoxy-D-manno-octulosonate
diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
-
-
-
r
CTP + 3-deoxy-D-manno-octulosonate
diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
-
-
r
CTP + 3-deoxy-D-manno-octulosonate
diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
-
-
r
CTP + 3-deoxy-D-manno-octulosonate
diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
-
-
?
CTP + 3-deoxy-D-manno-octulosonate
diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
involved in the expression of the capsular K5 polysaccharide K-CKS
-
-
r
CTP + 3-deoxy-D-manno-octulosonate
diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
biosynthesis of cell wall lipopolysaccharide
-
-
r
dCTP + 3-deoxy-D-manno-octulosonate
diphosphate + dCMP-3-deoxy-D-manno-octulosonate
-
-
-
r
dCTP + 3-deoxy-D-manno-octulosonate
diphosphate + dCMP-3-deoxy-D-manno-octulosonate
-
reaction at pH 8.0
-
-
r
dCTP + 3-deoxy-D-manno-octulosonate
diphosphate + dCMP-3-deoxy-D-manno-octulosonate
-
reaction at pH 8.0 and 9.5
-
-
r
UTP + 3-deoxy-D-manno-octulosonate
diphosphate + UMP-3-deoxy-D-manno-octulosonate
-
reaction at pH 9.5, approximately 50% the rate with CTP
-
-
r
UTP + 3-deoxy-D-manno-octulosonate
diphosphate + UMP-3-deoxy-D-manno-octulosonate
reaction at pH 9.5, approximately 50% the rate with CTP
-
-
r
UTP + 3-deoxy-D-manno-octulosonate
diphosphate + UMP-3-deoxy-D-manno-octulosonate
-
reaction at pH 9.5, to a lesser extent at pH 8.0
-
-
r
additional information
?
-
-
dUTP is no nucleotide acceptor for 3-deoxy-D-manno-octulosonate, 3-deoxy-D-manno-octulosonate-8-phosphate will not serve as substrate in place of 3-deoxy-D-manno-octulosonate
-
-
?
additional information
?
-
-
L-KDN is no substrate
-
-
?
additional information
?
-
-
no other sugar can replace 3-deoxy-D-manno-octulosonate, 3-deoxy-D-manno-octulosonate-8-phosphate and N-acetylneuraminic acid will not serve as substrates, neither dUTP nor any other nucleotide triphosphates, diphosphates or monophosphates like ATP, dATP, GTP, dGTP, ITP, dITP, TTP, CDP, UDP or CMP can substitute for CTP
-
-
?
additional information
?
-
-
3-deoxy-D-manno-octulosonate-8-phosphate is no substrate, neither NeuAc nor 3-deoxy-arabinoheptulosonate or 3-deoxy-erythro-hexulosonate are substrates
-
-
?
additional information
?
-
3-deoxy-D-manno-octulosonate-8-phosphate is no substrate, neither NeuAc nor 3-deoxy-arabinoheptulosonate or 3-deoxy-erythro-hexulosonate are substrates
-
-
?
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CTP + 3-deoxy-D-manno-octulosonate
diphosphate + CMP-3-deoxy-D-manno-octulosonate
CTP + 3-deoxy-D-manno-octulosonate
diphosphate + CMP-3-deoxy-D-manno-octulosonate
CTP + 3-deoxy-D-manno-octulosonate
diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
-
-
r
CTP + 3-deoxy-D-manno-octulosonate
diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
-
-
r
CTP + 3-deoxy-D-manno-octulosonate
diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
-
-
-
r
CTP + 3-deoxy-D-manno-octulosonate
diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
-
-
r
CTP + 3-deoxy-D-manno-octulosonate
diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
-
-
r
CTP + 3-deoxy-D-manno-octulosonate
diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
-
-
?
CTP + 3-deoxy-D-manno-octulosonate
diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
involved in the expression of the capsular K5 polysaccharide K-CKS
-
-
r
CTP + 3-deoxy-D-manno-octulosonate
diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
biosynthesis of cell wall lipopolysaccharide
-
-
r
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additional information
-
monovalent cations Na+, Li+ andK+ are ineffective in stimulating the enzyme, as are divalent cations Hg2+, Ni2+, and Fe2+
Ba2+
can partially substitute Mg2+
Ba2+
-
activation, 20% as effective as Mg2+
Ca2+
can partially substitute Mg2+
Ca2+
-
activation, 14% as effective as Mg2+
Cd2+
can partially substitute Mg2+
Cd2+
-
activation, 42% as effective as Mg2+
Co2+
-
much less effective than Mg2+
Co2+
can partially substitute Mg2+
Co2+
-
activation, 11% as effective as Mg2+
Mg2+
required
Mg2+
-
absolute requirement for a divalent cation
Mg2+
-
isoenzymes K-CKS and L-CKS, enzymatic activity critically dependent on magnesium ions
Mg2+
-
requires 10 mM for maximal activity, other divalent cations including Mn2+ and Co2+ are much less effective
Mg2+
-
optimum activity at 10 mM Mg2+
Mn2+
-
much less effective than Mg2+
Mn2+
can partially substitute Mg2+
Mn2+
-
activation, 38% as effective as Mg2+
Zn2+
can partially substitute Mg2+
Zn2+
-
activation, 32% as effective as Mg2+
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Ghalambor, M.A.; Heath, E.H.
The biosynthesis of cell wall lipopolysaccharide in Escherichia coli. IV. Purification and properties of cytidine monophosphate 3-deoxy-D-manno-octulosonate synthetase
J. Biol. Chem.
241
3216-3221
1966
Escherichia coli, Escherichia coli 0111-B4
brenda
Ray, P.H.; Benedict, C.D.; Grasmuk, H.
Purification and characterization of cytidine 5-triphosphate:cytidine 5-monophosphate-3-deoxy-D-manno-octulosonate cytidylyltransferase
J. Bacteriol.
145
1273-1280
1981
Escherichia coli, Escherichia coli B / ATCC 11303
brenda
Ray, P.H.; Benedict, C.D.
CTP:CMP-3-deoxy-D-manno-octulosonate cytidylyltransferase (CMP-KDO synthetase)
Methods Enzymol.
83
535-540
1982
Escherichia coli, Escherichia coli B / ATCC 11303
brenda
Claesson, A.; Luthman, K.; Gustafsson, K.; Bondesson, G.
A 2-deoxy analogue of KDO as the first inhibitor of the enzyme CMP-KDO synthetase
Biochem. Biophys. Res. Commun.
143
1063-1068
1987
Escherichia coli, Escherichia coli D21, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium SL 1102
brenda
Rosenow, C.; Roberts, I.S.; Jann, K.
Isolation from recombinant Escherichia coli and characterization of CMP-Kdo synthetase, involved in the expression of the capsular K5 polysaccharide (K-CKS)
FEMS Microbiol. Lett.
125
159-164
1995
Escherichia coli
brenda
Sugai, T.; Lin, C.H.; Shen, G.J.; Wong, C.H.
CMP-KDO synthetase: overproduction and application to the synthesis of CMP-KDO and analogs
Bioorg. Med. Chem.
3
313-320
1995
Escherichia coli
brenda
Jelakovic, S.; Jann, K.; Schulz, G.E.
The three-dimensional structure of capsule-specific CMP: 2-keto-3-deoxy-manno-octonic acid synthetase from Escherichia coli
FEBS Lett.
391
157-161
1996
Escherichia coli
brenda
Royo, J.; Gomez, E.; Hueros, G.
CMP-KDO synthetase. A plant gene borrowed from Gram-negative eubacteria
Trends Genet.
16
432-433
2000
Aggregatibacter actinomycetemcomitans, Aquifex pyrophilus, Arabidopsis thaliana, Chlamydia pneumoniae, Chlamydia trachomatis, Chlorobaculum tepidum, Escherichia coli, Haemophilus influenzae, Helicobacter pylori, Homo sapiens, Neisseria meningitidis, no activity in Caenorhabditis elegans, no activity in Drosophila melanogaster, no activity in Saccharomyces cerevisiae, Rickettsia prowazekii, Solanum lycopersicum, Vibrio cholerae serotype O1, Yersinia pestis, Zea mays
brenda
Jelakovic, S.; Schulz, G.E.
The structure of CMP:2-keto-3-deoxy-manno-octonic acid synthetase and of its complexes with substrates and substrate analogs
J. Mol. Biol.
312
143-155
2001
Escherichia coli, Escherichia coli (P42216)
brenda
Bravo, I.G.; Reglero, A.
The cytidyltransferases family: Properties, kinetics, genomic and phylogeny
Recent Res. Devel. Biochem.
4
224-254
2003
Aquifex aeolicus, Arabidopsis thaliana (Q9C920), Caulobacter vibrioides (Q9A4A3), Chlamydia muridarum, Chlamydia pneumoniae, Chlamydia trachomatis, Escherichia coli, Escherichia coli (P42216), Haemophilus influenzae, Helicobacter pylori, Mesorhizobium loti (Q98BN3), Neisseria meningitidis, Neisseria meningitidis (Q9K0D6), Neisseria meningitidis MC58 (Q9K0D6), Pasteurella multocida, Pseudomonas aeruginosa, Rickettsia conorii (Q92I96), Rickettsia prowazekii, Salmonella enterica subsp. enterica serovar Typhimurium, Sinorhizobium meliloti (Q92SX6), Vibrio cholerae (Q9KQX2), Xylella fastidiosa (Q9PB46), Zea mays, Zea mays (Q9M4D3), Zea mays (Q9M4G3)
-
brenda
Kondo, K.; Doi, H.; Adachi, H.; Nishimura, Y.
Synergistic effect of CMP/KDO synthase inhibitors with antimicrobial agents on inhibition of production and release of vero toxin by enterohaemorrhagic Escherichia coli O157:H7
Bioorg. Med. Chem. Lett.
14
467-470
2004
Escherichia coli
brenda
Yi, L.; Velasquez, M.S.; Holler, T.P.; Woodard, R.W.
A simple assay for 3-deoxy-D-manno-octulosonate cytidylyltransferase and its use as a pathway screen
Anal. Biochem.
416
152-158
2011
Escherichia coli
brenda
Wen, L.; Zheng, Y.; Li, T.; Wang, P.G.
Enzymatic synthesis of 3-deoxy-D-manno-octulosonic acid (KDO) and its application for LPS assembly
Bioorg. Med. Chem. Lett.
26
2825-2828
2016
Escherichia coli (P04951)
brenda
Nilsson, I.; Grove, K.; Dovala, D.; Uehara, T.; Lapointe, G.; Six, D.A.
Molecular characterization and verification of azido-3,8-dideoxy-D-manno-oct-2-ulosonic acid incorporation into bacterial lipopolysaccharide
J. Biol. Chem.
292
19840-19848
2017
Escherichia coli (P04951)
brenda