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Information on EC 2.7.7.35 - ADP ribose phosphorylase for references in articles please use BRENDA:EC2.7.7.35
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EC Tree
IUBMB Comments The enzyme, characterized from the single-celled alga Euglena gracilis, catalyses an irreversible reaction in the direction of ADP formation. cf. EC 2.7.7.96, ADP-D-ribose pyrophosphorylase.
The enzyme appears in viruses and cellular organisms
Synonyms
adenosine diphosphoribose phosphorylase, adenylyltransferase, ribose 5-phosphate, ADP ribose phosphorylase,
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adenosine diphosphoribose phosphorylase
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adenylyltransferase, ribose 5-phosphate
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ADP ribose phosphorylase
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ADP + D-ribose 5-phosphate = phosphate + ADP-D-ribose
ADP + D-ribose 5-phosphate = phosphate + ADP-D-ribose
ping-pong reaction mechanism
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ADP + D-ribose 5-phosphate = phosphate + ADP-D-ribose
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nucleotidyl group transfer
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ADP:D-ribose-5-phosphate adenylyltransferase
The enzyme, characterized from the single-celled alga Euglena gracilis, catalyses an irreversible reaction in the direction of ADP formation. cf. EC 2.7.7.96, ADP-D-ribose pyrophosphorylase.
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phosphate + ADP
ADP + phosphate
phosphate + ADP-ribose
ADP + D-ribose 5-phosphate
phosphate + deamino-NAD+
?
additional information
?
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phosphate + ADP
ADP + phosphate
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ADP/phosphate-exchange reaction, inorganic phosphate is incorporated into terminal position of ADP
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r
phosphate + ADP
ADP + phosphate
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IDP can replace ADP with 16% efficiency
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r
phosphate + ADP
ADP + phosphate
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IDP can replace ADP with 16% efficiency
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r
phosphate + ADP
ADP + phosphate
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IDP can replace ADP with 16% efficiency
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r
phosphate + ADP-ribose
ADP + D-ribose 5-phosphate
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phosphate + ADP-ribose
ADP + D-ribose 5-phosphate
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ir
phosphate + ADP-ribose
ADP + D-ribose 5-phosphate
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ir
phosphate + ADP-ribose
ADP + D-ribose 5-phosphate
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highly specific
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ir
phosphate + ADP-ribose
ADP + D-ribose 5-phosphate
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highly specific
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ir
phosphate + ADP-ribose
ADP + D-ribose 5-phosphate
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phosphate + ADP-ribose
ADP + D-ribose 5-phosphate
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phosphate + ADP-ribose
ADP + D-ribose 5-phosphate
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ir
phosphate + deamino-NAD+
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inosine diphosphoribose, not adenosine triphosphoribose
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phosphate + deamino-NAD+
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inosine diphosphoribose, not adenosine triphosphoribose
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?
additional information
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no activity with CDP, GDP, UDP, ADP-glucose and UDP-glucose
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additional information
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no activity with CDP, GDP, UDP, ADP-glucose and UDP-glucose
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additional information
?
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no activity with crude polyadenylic acid, RNA from Euglena, and NAD(H), NADP(H), coenzyme A, FAD
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additional information
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no activity with AMP and ATP
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phosphate + ADP-ribose
ADP + D-ribose 5-phosphate
phosphate + ADP-ribose
ADP + D-ribose 5-phosphate
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phosphate + ADP-ribose
ADP + D-ribose 5-phosphate
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ir
phosphate + ADP-ribose
ADP + D-ribose 5-phosphate
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ir
phosphate + ADP-ribose
ADP + D-ribose 5-phosphate
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phosphate + ADP-ribose
ADP + D-ribose 5-phosphate
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ir
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additional information
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no Mn2+ or Mg2+ requirement
additional information
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no metal ion requirement
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ADP
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weak, ADP-ribose phosphorolysis
ADP-ribose
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competitive to phosphate
deoxyribose 5-phosphate
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ADP/phosphate-exchange
IDP
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weak, ADPribose phosphorolysis
AMP
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kinetics
arsenate
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kinetics
ATP
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kinetics
D-ribose 5-phosphate
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competitive to phosphate with ADP, noncompetitive to phosphate with ADP-ribose
D-ribose 5-phosphate
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kinetics
PCMB
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ADP/phosphate-exchange, reversible by cysteine
PCMB
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ADP/phosphate-exchange and ADPribose phosphorolysis
additional information
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no inhibition by CN-, 1,10-phenanthroline, 2,2'-dipyridyl, ribose 1-phosphate, ribose 1,5-diphosphate, glucose 1-phosphate, glucose 6-phosphate; no inhibition by NADP+, EDTA, ribose
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additional information
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no inhibition by adenosine, deoxyadenosine, 3'-AMP, CMP, CDP, UDP, NADH, NAD+, ADPglucose, UDPglucose, IAA, NEM; no inhibition by NADP+, EDTA, ribose
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additional information
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additional information
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no activation by EDTA
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additional information
additional information
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kinetic study
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0.6
ADP
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ADP/phosphate exchange; pH 7.8, 30°C
0.6
ADP
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ADP/phosphate exchange
0.04
ADP-ribose
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pH 7.8, 30°C
0.05
ADP-ribose
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pH 7.8, 35°C
0.4
phosphate
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pH 7.8, 30°C
0.5
phosphate
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pH 7.8, 35°C
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0.09 - 4
D-ribose 5-phosphate
1
AMP
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pH 7.8, 35°C; phosphorolysis
1
AMP
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pH 7.8, 30°C; phosphorolysis
1.5
AMP
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ADP/phosphate-exchange reaction; pH 7.8, 35°C
1.5
AMP
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ADP/phosphate-exchange reaction; pH 7.8, 30°C
0.41
arsenate
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ADP/phosphate-exchange reaction; pH 7.8, 35°C
0.41
arsenate
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ADP/phosphate-exchange reaction; pH 7.8, 30°C
0.57
arsenate
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pH 7.8, 35°C; phosphorolysis
0.57
arsenate
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pH 7.8, 30°C; phosphorolysis
1.3
ATP
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ADP/phosphate-exchange reaction; pH 7.8, 35°C
1.3
ATP
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ADP/phosphate-exchange reaction; pH 7.8, 30°C
2.9
ATP
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pH 7.8, 35°C; phosphorolysis
2.9
ATP
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pH 7.8, 30°C; phosphorolysis
0.09
D-ribose 5-phosphate
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pH 7.8, 35°C; phosphorolysis
0.09
D-ribose 5-phosphate
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pH 7.8, 30°C; phosphorolysis
0.11
D-ribose 5-phosphate
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ADP/phosphate-exchange reaction; pH 7.8, 30°C
0.11
D-ribose 5-phosphate
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ADP/phosphate-exchange reaction; pH 7.8, 35°C
0.11
D-ribose 5-phosphate
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ADP/phosphate-exchange reaction; pH 7.8, 30°C
4
D-ribose 5-phosphate
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phosphorolysis, pH 7.8, 30°C
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0.51
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partially purified enzyme
1.3
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partially purified enzyme
additional information
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low activity
additional information
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low activity
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7.5 - 7.8
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ADP/phosphate-exchange
7.5
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ADP/phosphate-exchange
8
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ADP-ribose phosphorolysis
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6.4 - 8.1
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ADP/phosphate-exchange, about half-maximal activity at pH 6.4 and pH 8.1
6.7 - 8.5
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ADP/phosphate-exchange, about 60% of maximal activity at pH 6.7 and about half-maximal activity at pH 8.5
6.8 - 9
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ADP-ribose phosphorolysis, about half-maximal activity at pH 6.8 and about 90% of maximal activity at pH 9.0
6.8 - 9.4
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about half-maximal activity at pH 6.8 and pH 9.4
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29 - 56
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about half-maximal activity at 29°C and 56°C
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no activity in Chlorella pyrenoidosa
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brenda
no activity in Chlorella sp.
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brenda
no activity in Gloeocapsa sp.
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brenda
no activity in Pisum sativum
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brenda
no activity in Spinacia oleracea
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brenda
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brenda
green alga, strain Z
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brenda
var. bacillaris Pringsheim
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brenda
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heterotrophically or autotrophically grown cells; UV-mutant cells incapable of forming chloroplasts
brenda
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heterotrophically or autotrophically grown cells
brenda
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brenda
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brenda
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brenda
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solution of lyophilized enzyme, 2 mg/ml, stable to repeated freeze-thaw cycles during 2 weeks
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-15°C, undialyzed purified enzyme, lyophilized and placed under vacuum, 35% loss of activity within 2 months
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frozen, fairly stable in the presence of EDTA
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Evans, W.R.; Pietro, A.S.
Phosphorolysis of adenosine diphosphoribose
Arch. Biochem. Biophys.
113
236-244
1966
Euglena gracilis, no activity in Chlorella sp., no activity in Gloeocapsa sp., no activity in Pisum sativum, no activity in Spinacia oleracea, Ochromonas danica
brenda
Stern, A.I.; Avron, M.
An adenosine 5-diphosphate ribose:orthophosphate adenylyltransferase from Euglena gracilis
Biochim. Biophys. Acta
118
577-591
1966
Euglena gracilis, no activity in Chlorella pyrenoidosa
brenda
Evans, W.R.
Adenosine diphosphoribose phosphorylase from Euglena gracilis
Methods Enzymol.
23A
566-570
1971
Euglena gracilis, Ochromonas danica
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brenda
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2.7.7.35 ADP ribose phosphorylase
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