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Information on EC 2.7.7.3 - pantetheine-phosphate adenylyltransferase and Organism(s) Pyrococcus abyssi and UniProt Accession Q9UYT0

for references in articles please use BRENDA:EC2.7.7.3
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EC Tree
IUBMB Comments
The enzyme from several bacteria (e.g. Escherichia coli, Bacillus subtilis and Haemophilus influenzae) has been shown to be bifunctional and also to possess the activity of EC 2.3.1.157, glucosamine-1-phosphate N-acetyltransferase.
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This record set is specific for:
Pyrococcus abyssi
UNIPROT: Q9UYT0
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Word Map
The taxonomic range for the selected organisms is: Pyrococcus abyssi
The enzyme appears in selected viruses and cellular organisms
Synonyms
phosphopantetheine adenylyltransferase, 4'-phosphopantetheine adenylyltransferase, pantetheine phosphate adenylyltransferase, enterococcus faecalis ppat, pantetheine-phosphate adenylyltransferase, dephospho-coa pyrophosphorylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3'-dephospho-CoA pyrophosphorylase
-
-
-
-
dephospho-CoA pyrophosphorylase
-
-
-
-
dephospho-coenzyme A pyrophosphorylase
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-
-
-
pantetheine phosphate adenylyltransferase
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-
-
-
PPAT
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
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-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -
SYSTEMATIC NAME
IUBMB Comments
ATP:pantetheine-4'-phosphate adenylyltransferase
The enzyme from several bacteria (e.g. Escherichia coli, Bacillus subtilis and Haemophilus influenzae) has been shown to be bifunctional and also to possess the activity of EC 2.3.1.157, glucosamine-1-phosphate N-acetyltransferase.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-99-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + pantetheine 4'-phosphate
diphosphate + 3'-dephospho-CoA
show the reaction diagram
ATP + pantetheine 4'-phosphate
diphosphate + 3'-dephospho-CoA
show the reaction diagram
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-
-
-
r
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + pantetheine 4'-phosphate
diphosphate + 3'-dephospho-CoA
show the reaction diagram
rate-limiting penultimate step in coenzyme A biosynthesis
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-
r
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.07
reverse reaction using the purified His-tag fusion protein
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
19990
MALDI-TOF mass spectrometry of His-tag fusion protein
19992
1 * 20000, SDS-PAGE of His-tag fusion protein, circular dichroism, and 2D NMR experiments, 1 * 19992, MALDI-TOF mass spectrometry
20000
SDS-PAGE of His-tag fusion protein
20000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
1 * 20000, SDS-PAGE of His-tag fusion protein, circular dichroism, and 2D NMR experiments, 1 * 19992, MALDI-TOF mass spectrometry
monomer
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1 * 20000, gel filtration
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant protein using His-tag
to homogenity
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of native gene in Escherichia coli BL21(DE3) not possible, expression of optimized gene in Escherichia coli BL21(DE3) as His-tag fusion protein
overexpression in Escherichia coli
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Armengaud, J.; Fernandez, B.; Chaumont, V.; Rollin-Genetet, F.; Finet, S.; Marchetti, C.; Myllykallio, H.; Vidaud, C.; Pellequer, J.L.; Gribaldo, S.; Forterre, P.; Gans, P.
Identification, purification, and characterization of an eukaryotic-like phosphopantetheine adenylyltransferase (coenzyme A biosynthetic pathway) in the hyperthermophilic archaeon Pyrococcus abyssi
J. Biol. Chem.
278
31078-31087
2003
Pyrococcus abyssi
Manually annotated by BRENDA team
Nalezkova, M.; de Groot, A.; Graf, M.; Gans, P.; Blanchard, L.
Overexpression and purification of Pyrococcus abyssi phosphopantetheine adenylyltransferase from an optimized synthetic gene for NMR studies
Protein Expr. Purif.
39
296-306
2005
Pyrococcus abyssi (Q9UYT0), Pyrococcus abyssi
Manually annotated by BRENDA team