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Information on EC 2.7.7.19 - polynucleotide adenylyltransferase and Organism(s) Arabidopsis thaliana and UniProt Accession O82312

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IUBMB Comments
Also acts slowly with CTP. Catalyses template-independent extension of the 3'- end of a DNA strand by one nucleotide at a time. Cannot initiate a chain de novo. The primer, depending on the source of the enzyme, may be an RNA or DNA fragment, or oligo(A) bearing a 3'-OH terminal group. See also EC 2.7.7.6 DNA-directed RNA polymerase.
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Arabidopsis thaliana
UNIPROT: O82312
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
poly(a) polymerase, pap i, fam46c, gld-2, star-pap, fam46a, poly(a) polymerase i, pap ii, papd5, mtpap, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
poly(A) polymerase
adenosine triphosphate:ribonucleic acid adenylyltransferase
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-
-
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AMP polynucleotidylexotransferase
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-
-
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ATP-polynucleotide adenylyltransferase
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-
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ATP:polynucleotidylexotransferase
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-
-
-
neo-PAP
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-
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NTP polymerase
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-
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nuclear poly(A) polymerase 1
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nucleotidyltransferase, polyadenylate
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-
-
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PAP
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-
-
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PAP I
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-
-
-
PAPS1
PAPS4
poly(A) hydrolase
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-
-
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poly(A) polymerase
poly(A) synthetase
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-
-
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polyadenylate nucleotidyltransferase
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-
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polyadenylate polymerase
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-
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polyadenylate synthetase
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-
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polyadenylic acid polymerase
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polyadenylic polymerase
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-
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RNA adenylating enzyme
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-
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RNA formation factors, PF1
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-
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terminal riboadenylate transferase
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-
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-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + RNAn = diphosphate + RNAn+1
show the reaction diagram
Arabidopsis possesses 4 genes for PAP, these genes are expressed at the level of mRNA in tissue-specific ways, transcripts from the four genes are alternatively spliced so as to yield mRNAs encoding highly truncated polypeptides
O82312, Q7XJ91, Q7XJ92, Q9LMT2
ATP + RNAn = diphosphate + RNAn+1
show the reaction diagram
Arabidopsis possesses 4 genes for PAP, these genes are expressed at the level of mRNA in tissue-specific ways, transcripts from the four genes are alternatively spliced so as to yield mRNAs encoding highly truncated polypeptides
O82312, Q7XJ91, Q7XJ92, Q9LMT2
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:polynucleotide adenylyltransferase
Also acts slowly with CTP. Catalyses template-independent extension of the 3'- end of a DNA strand by one nucleotide at a time. Cannot initiate a chain de novo. The primer, depending on the source of the enzyme, may be an RNA or DNA fragment, or oligo(A) bearing a 3'-OH terminal group. See also EC 2.7.7.6 DNA-directed RNA polymerase.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-30-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + RNA
diphosphate + RNA(A)n
show the reaction diagram
-
-
-
?
ATP + RNAn
diphosphate + RNAn+1
show the reaction diagram
-
-
-
?
ATP + RNAn
diphosphate + RNAn+1
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + RNAn
diphosphate + RNAn+1
show the reaction diagram
-
-
-
?
ATP + RNAn
diphosphate + RNAn+1
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
more active in presence of Mg2+ than Mn2+
Mn2+
more active in presence of Mg2+ than Mn2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
100% inhibition at 0.5 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
O82312, Q7XJ91, Q7XJ92, Q9LMT2
-
Manually annotated by BRENDA team
O82312, Q7XJ91, Q7XJ92, Q9LMT2
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Manually annotated by BRENDA team
O82312, Q7XJ91, Q7XJ92, Q9LMT2
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Manually annotated by BRENDA team
O82312, Q7XJ91, Q7XJ92, Q9LMT2
-
Manually annotated by BRENDA team
O82312, Q7XJ91, Q7XJ92, Q9LMT2
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
isoform PAPS promotes flowering in a partially redundant manner. The enzymes act antagonistically to isoform PAPS1, which delays the transition to flowering
malfunction
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PAPS2_ARATH
800
0
90968
Swiss-Prot
other Location (Reliability: 2)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hunt, A.G.; Meeks, L.R.; Forbes, K.P.; Das Gupta, J.; Mogen, B.D.
Nuclear and chloroplast poly(A) polymerases from plants share a novel biochemical property
Biochem. Biophys. Res. Commun.
272
174-181
2000
Arabidopsis thaliana (O82312)
Manually annotated by BRENDA team
Addepalli, B.; Meeks, L.R.; Forbes, K.P.; Hunt, A.G.
Novel alternative splicing of mRNAs encoding poly(A) polymerases in Arabidopsis
Biochim. Biophys. Acta
1679
117-128
2004
Arabidopsis thaliana (O82312), Arabidopsis thaliana (Q7XJ91), Arabidopsis thaliana (Q7XJ92), Arabidopsis thaliana (Q9LMT2), Arabidopsis thaliana
Manually annotated by BRENDA team
Trost, G.; Vi, S.L.; Czesnick, H.; Lange, P.; Holton, N.; Giavalisco, P.; Zipfel, C.; Kappel, C.; Lenhard, M.
Arabidopsis poly(A) polymerase PAPS1 limits founder-cell recruitment to organ primordia and suppresses the salicylic acid-independent immune response downstream of EDS1/PAD4
Plant J.
77
688-699
2014
Arabidopsis thaliana (Q9LMT2), Arabidopsis thaliana
Manually annotated by BRENDA team
Vi, S.L.; Trost, G.; Lange, P.; Czesnick, H.; Rao, N.; Lieber, D.; Laux, T.; Gray, W.M.; Manley, J.L.; Groth, D.; Kappel, C.; Lenhard, M.
Target specificity among canonical nuclear poly(A) polymerases in plants modulates organ growth and pathogen response
Proc. Natl. Acad. Sci. USA
110
13994-13999
2013
Arabidopsis thaliana (Q8VYW1), Arabidopsis thaliana (Q9LMT2)
Manually annotated by BRENDA team
Czesnick, H.; Lenhard, M.
Antagonistic control of flowering time by functionally specialized poly(A) polymerases in Arabidopsis thaliana
Plant J.
88
570-583
2016
Arabidopsis thaliana (O82312), Arabidopsis thaliana (Q8YYW1), Arabidopsis thaliana (Q9LMT2), Arabidopsis thaliana
Manually annotated by BRENDA team
Zhang, Y.; Ramming, A.; Heinke, L.; Altschmied, L.; Slotkin, R.K.; Becker, J.D.; Kappel, C.; Lenhard, M.
The poly(A) polymerase PAPS1 interacts with the RNA-directed DNA-methylation pathway in sporophyte and pollen development
Plant J.
99
655-672
2019
Arabidopsis thaliana (Q9LMT2), Arabidopsis thaliana
Manually annotated by BRENDA team