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Information on EC 2.7.7.14 - ethanolamine-phosphate cytidylyltransferase
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2.7.7.14 ethanolamine-phosphate cytidylyltransferaseSpecify your search results
Word Map
- 2.7.7.14
- phosphatidylethanolamine
- phospholipid
- cdp-ethanolamine
- diacylglycerols
-
kennedy
- cdp-choline
-
ctp:phosphocholine
- phosphocholine
-
3hethanolamine
-
ptdetn
- ethanolaminephosphotransferase
-
14cethanolamine
- drug development
The enzyme appears in viruses and cellular organisms
Synonyms
pcyt2, ctp:phosphoethanolamine cytidylyltransferase, pect1, phosphoethanolamine cytidylyltransferase, ethanolamine-phosphate cytidylyltransferase, ctp:ethanolaminephosphate cytidylyltransferase, ctp:phosphoethanolamine cytidylyltransferase gene, pcyt2beta, pcyt2alpha, ctp-phosphoethanolamine cytidylyltransferase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
CTP-phosphoethanolamine cytidylyltransferase
-
-
-
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CTP:phosphoethanolamine cytidylyltransferase
cytidylyltransferase, ethanolamine phosphate
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-
-
-
ECT
ET
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-
-
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ethanolamine phosphate cytidylyltransferase
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-
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ethanolamine-phosphate cytidylyltransferase
Pcyt2
Pcyt2alpha
Pcyt2beta
PECT1
phosphoethanolamine cytidylyltransferase
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-
-
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phosphorylethanolamine transferase
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-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
CTP + ethanolamine phosphate = diphosphate + CDP-ethanolamine
ordered reaction mechanism
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CTP + ethanolamine phosphate = diphosphate + CDP-ethanolamine
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
nucleotidyl group transfer
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
CTP:ethanolamine-phosphate cytidylyltransferase
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CAS REGISTRY NUMBER
COMMENTARY
9026-33-9
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
CTP + 2-aminoethylarsonic acid
diphosphate + cytidine-O-PO2-O-AsO2-CH2-CH2-NH3+
-
-
spontaneous hydrolysis to CMP and-O-AsO2--CH2-CH2-NH3+
?
CTP + phosphodimethylethanolamine
diphosphate + CDP-dimethylethanolamine
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-
-
-
?
CTP + phosphomonomethylethanolamine
diphosphate + CDP-methylethanolamine
-
-
-
-
?
CTP + ethanolamine phosphate
CDP-ethanolamine + diphosphate
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-
-
-
?
CTP + ethanolamine phosphate
CDP-ethanolamine + diphosphate
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-
-
?
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
-
rate limiting enzyme in phosphatidylethanolamine biosynthesis. Defects in CTP:phosphorylethanolamine cytidylyltransferase affect embryonic and postembryonic development
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-
?
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
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-
-
?
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
enzyme activity increases during the dark and then decreases during the light period, while the mRNA level does not alter, providing evidence for posttranslational regulation
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-
?
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
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-
-
-
?
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
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-
-
?
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
-
Pcyt2is necessary for murine development. A single Pcyt2 allele in heterozygotes can maintain phospholipid homeostasis
-
-
?
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
-
-
-
?
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
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-
-
-
?
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
-
rate-regulatory enzyme of phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway
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-
?
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
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catalyzes a central step in phosphatidylethanolamine synthesis
-
r
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
-
catalyzes a central step in phosphatidylethanolamine synthesis
-
r
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
-
at physiological ethanolamine concentrations, the supply of CDPethanolamine, via the expression level of CTP:phosphoethanolamine cytidylyltransferase, together with the amount of cellular diacylglycerol available to CTP:phosphoethanolamine cytidylyltransferase are major factors regulating the flux through the CDPethanolamine pathway
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-
?
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
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-
-
-
?
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
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-
-
-
?
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
-
-
-
-
?
dCTP + ethanolamine phosphate
diphosphate + dCDP-ethanolamine
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-
-
?
dCTP + ethanolamine phosphate
diphosphate + dCDP-ethanolamine
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-
-
-
?
dCTP + ethanolamine phosphate
diphosphate + dCDP-ethanolamine
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-
-
-
?
additional information
?
-
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uses N-methylated derivatives of ethanolamine phosphate less efficiently and does not react with phosphocholine
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-
?
additional information
?
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catalytic reaction of ECT obeys Michaelis-Menten kinetics with respect to both CTP and phosphoethanolamine
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-
?
additional information
?
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catalytic reaction of ECT obeys Michaelis-Menten kinetics with respect to both CTP and phosphoethanolamine
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?
additional information
?
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Pcyt2 is the main regulatory enzyme in the CDP-ethanolamine pathwa
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?
additional information
?
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uses N-methylated derivatives of ethanolamine phosphate less efficiently and does not react with phosphocholine
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-
?
additional information
?
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uses N-methylated derivatives of ethanolamine phosphate less efficiently and does not react with phosphocholine
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-
?
additional information
?
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the enzyme shows high substrate specificity for ethanolamine phosphate
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?
additional information
?
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uses N-methylated derivatives of ethanolamine phosphate less efficiently and does not react with phosphocholine
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?
additional information
?
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the enzyme shows high substrate specificity for ethanolamine phosphate
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-
?
additional information
?
-
-
the enzyme shows high substrate specificity for ethanolamine phosphate
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-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
Pcyt2 is the main regulatory enzyme in the CDP-ethanolamine pathwa
-
-
?
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
-
rate limiting enzyme in phosphatidylethanolamine biosynthesis. Defects in CTP:phosphorylethanolamine cytidylyltransferase affect embryonic and postembryonic development
-
-
?
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
enzyme activity increases during the dark and then decreases during the light period, while the mRNA level does not alter, providing evidence for posttranslational regulation
-
-
?
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
-
Pcyt2is necessary for murine development. A single Pcyt2 allele in heterozygotes can maintain phospholipid homeostasis
-
-
?
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
-
-
-
?
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
-
-
-
-
?
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
-
rate-regulatory enzyme of phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway
-
-
?
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
-
catalyzes a central step in phosphatidylethanolamine synthesis
-
r
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
-
catalyzes a central step in phosphatidylethanolamine synthesis
-
r
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
-
at physiological ethanolamine concentrations, the supply of CDPethanolamine, via the expression level of CTP:phosphoethanolamine cytidylyltransferase, together with the amount of cellular diacylglycerol available to CTP:phosphoethanolamine cytidylyltransferase are major factors regulating the flux through the CDPethanolamine pathway
-
-
?
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
-
-
-
-
?
CTP + ethanolamine phosphate
diphosphate + CDP-ethanolamine
-
-
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Mn2+
Mn2+
-
activation, optimal concentration: 2-4 mM, 50% of the activity with Mg2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1-Aminoethylphosphonate
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inhibitory power stimulated by Mg2+; noncompetitive to phosphorylethanolamine
2-aminoethylphosphonate
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competitive to phosphorylethanolamine; inhibitory power stimulated by Mg2+
3-Aminopropylphosphonate
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competitive to phosphorylethanolamine; inhibitory power stimulated by Mg2+
Sphingosine/phosphatidylcholine vesicles
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inhibit cytosolic and purified enzyme
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additional information
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overactivation of the NMDA receptor in neurons inhibits the enzyme, membrane damage by NMDA receptor is preceeded by inhibition of phospholipid synthesis
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phosphoethanolamine methyl-analogues
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weak competitive inhibitors of Pcyt2
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phosphoethanolamine methyl-analogues
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weak competitive inhibitors of Pcyt2
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phosphoethanolamine methyl-analogues
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weak competitive inhibitors of Pcyt2
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phosphoethanolamine methyl-analogues
-
weak competitive inhibitors of Pcyt2
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
early growth response protein 1
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important transcriptional stimulator of gene expression
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Reducing agent
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activity of purified enzyme is dependent on the presence of DTT
-
additional information
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does not require the presence of exogenous lipids for optimal activity
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additional information
does not require the presence of exogenous lipids for optimal activity
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additional information
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does not require the presence of exogenous lipids for optimal activity
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additional information
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addition of lipids does not stimulate the activity of cytosolic enzyme
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additional information
-
does not require the presence of exogenous lipids for optimal activity
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Atherosclerosis
Liver X Receptor Agonists Inhibit the Phospholipid Regulatory Gene CTP: Phosphoethanolamine Cytidylyltransferase-Pcyt2.
Breast Neoplasms
Breast cancer cells adapt to metabolic stress by increasing ethanolamine phospholipid synthesis and CTP:ethanolaminephosphate cytidylyltransferase-Pcyt2 activity.
Breast Neoplasms
Characterization of transcription factors and cis-acting elements that regulate human CTP: phosphoethanolamine cytidylyltransferase (Pcyt2).
Breast Neoplasms
Liver X Receptor Agonists Inhibit the Phospholipid Regulatory Gene CTP: Phosphoethanolamine Cytidylyltransferase-Pcyt2.
Breast Neoplasms
Phosphoethanolamine Accumulation Protects Cancer Cells under Glutamine Starvation through Downregulation of PCYT2.
Dwarfism
Defects in CTP:PHOSPHORYLETHANOLAMINE CYTIDYLYLTRANSFERASE affect embryonic and postembryonic development in Arabidopsis.
Epilepsy
Mutations in PCYT2 disrupt etherlipid biosynthesis and cause a complex hereditary spastic paraplegia.
ethanolamine-phosphate cytidylyltransferase deficiency
Choline supplementation restores substrate balance and alleviates complications of Pcyt2 deficiency.
ethanolamine-phosphate cytidylyltransferase deficiency
Mechanism of hypertriglyceridemia in CTP:phosphoethanolamine cytidylyltransferase-deficient mice.
ethanolamine-phosphate cytidylyltransferase deficiency
The development of a metabolic disease phenotype in CTP:phosphoethanolamine cytidylyltransferase-deficient mice.
Fatty Liver
Mechanism of hypertriglyceridemia in CTP:phosphoethanolamine cytidylyltransferase-deficient mice.
Fatty Liver
The development of a metabolic disease phenotype in CTP:phosphoethanolamine cytidylyltransferase-deficient mice.
Hypertriglyceridemia
Mechanism of hypertriglyceridemia in CTP:phosphoethanolamine cytidylyltransferase-deficient mice.
Hypertriglyceridemia
The development of a metabolic disease phenotype in CTP:phosphoethanolamine cytidylyltransferase-deficient mice.
Insulin Resistance
Mechanism of hypertriglyceridemia in CTP:phosphoethanolamine cytidylyltransferase-deficient mice.
Insulin Resistance
The development of a metabolic disease phenotype in CTP:phosphoethanolamine cytidylyltransferase-deficient mice.
Insulin Resistance
Type 2 diabetes-related proteins derived from an in vitro model of inflamed fat tissue.
Metabolic Syndrome
Mechanism of hypertriglyceridemia in CTP:phosphoethanolamine cytidylyltransferase-deficient mice.
Metabolic Syndrome
The development of a metabolic disease phenotype in CTP:phosphoethanolamine cytidylyltransferase-deficient mice.
Muscle Spasticity
Mutations in PCYT2 disrupt etherlipid biosynthesis and cause a complex hereditary spastic paraplegia.
Neoplasms
Breast cancer cells adapt to metabolic stress by increasing ethanolamine phospholipid synthesis and CTP:ethanolaminephosphate cytidylyltransferase-Pcyt2 activity.
Neoplasms
Phosphoethanolamine Accumulation Protects Cancer Cells under Glutamine Starvation through Downregulation of PCYT2.
Obesity
Mechanism of hypertriglyceridemia in CTP:phosphoethanolamine cytidylyltransferase-deficient mice.
Obesity
The development of a metabolic disease phenotype in CTP:phosphoethanolamine cytidylyltransferase-deficient mice.
Parkinson Disease
Elevated activity of phospholipid biosynthetic enzymes in substantia nigra of patients with Parkinson's disease.
Spastic Paraplegia, Hereditary
A novel PCYT2 mutation identified in a Chinese consanguineous family with hereditary spastic paraplegia.
Spastic Paraplegia, Hereditary
Mutations in PCYT2 disrupt etherlipid biosynthesis and cause a complex hereditary spastic paraplegia.
Starvation
Phosphoethanolamine Accumulation Protects Cancer Cells under Glutamine Starvation through Downregulation of PCYT2.
Starvation
Transcriptional suppression of CTP:phosphoethanolamine cytidylyltransferase by 25-hydroxycholesterol is mediated by nuclear factor-Y and Yin Yang 1.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.374
CTP
pH 8.0, 37°C, recombinant His-tagged mutant H146A enzyme
0.465
CTP
pH 8.0, 37°C, recombinant His-tagged mutant H422A enzyme
0.452
Ethanolamine phosphate
pH 8.0, 37°C, recombinant His-tagged wild-type enzyme
0.565
Ethanolamine phosphate
pH 8.0, 37°C, recombinant His-tagged mutant H422A enzyme
additional information
additional information
Michaelis-Menten kinetics of wild-type and mutant enzymes, overview
-
additional information
additional information
-
Michaelis-Menten kinetics of wild-type and mutant enzymes, overview
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3.4
Ethanolamine phosphate
pH 8.0, 37°C, recombinant His-tagged wild-type enzyme
4.1
Ethanolamine phosphate
pH 8.0, 37°C, recombinant His-tagged mutant H422A enzyme
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT