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Information on EC 2.7.7.13 - mannose-1-phosphate guanylyltransferase and Organism(s) Arabidopsis thaliana and UniProt Accession O22287

for references in articles please use BRENDA:EC2.7.7.13
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IUBMB Comments
The bacterial enzyme can also use ITP and dGTP as donors.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: O22287
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
gmppb, gdp-mannose pyrophosphorylase, vtc1-1, gdp-d-mannose pyrophosphorylase, gdp-mp, osvtc1-1, osvtc1-3, nspase, mannose-1-phosphate guanylyltransferase, gdp-man pyrophosphorylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GDP-D-mannose pyrophosphorylase
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GDP-mannose pyrophosphorylase
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VTC1
gene name, vitamin C defective 1
GDP-Man pyrophosphorylase
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GDP-mannose pyrophosphorylase
GTP-mannose 1-phosphate guanylyltransferase
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-
-
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GTP-mannose-1-phosphate guanylyltransferase
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-
-
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guanosine 5'-diphospho-D-mannose pyrophosphorylase
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-
-
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guanosine diphosphomannose pyrophosphorylase
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-
-
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guanosine triphosphate-mannose 1-phosphate guanylyltransferase
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-
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guanylyltransferase, mannose 1-phosphate
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mannose 1-phosphate guanylyltransferase (guanosine triphosphate)
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nucleotidyl group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
GTP:alpha-D-mannose-1-phosphate guanylyltransferase
The bacterial enzyme can also use ITP and dGTP as donors.
CAS REGISTRY NUMBER
COMMENTARY hide
37278-24-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
GTP + alpha-D-mannose 1-phosphate
diphosphate + GDP-D-mannose
show the reaction diagram
-
-
-
?
D-mannose 1-phosphate + GTP
GDP-D-mannose + diphosphate
show the reaction diagram
-
-
-
-
?
GTP + alpha-D-mannose 1-phosphate + GTP
diphosphate + GDP-alpha-D-mannose
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
GTP + alpha-D-mannose 1-phosphate
diphosphate + GDP-D-mannose
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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the ascorbic acid-deficient Arabidopsis thaliana mutant vtc1-1 is defective in GDP-mannose pyrophosphorylase and exhibits conditional hypersensitivity to ammonium, a phenomenon that is independent of ascorbic acid deficiency. Defective N-glycosylation in vtc1-1 contributes to cell wall, membrane and cell cycle defects, resulting in root growth inhibition in the presence of NH4+, GDP-mannose deficiency does not generally lead to and is not the primary cause of NH4+ sensitivity. GDP-mannose deficiency results in an up-regulation of ER stress genes, enhanced programmed cell death and defective cell cycle proliferation in vtc1-1 triggered by NH4+, overview. Root development in the presence of NH4+ is fully recovered in vtc1-1 containing one wild-type copy of VTC1
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GMPP1_ARATH
361
0
39577
Swiss-Prot
Mitochondrion (Reliability: 4)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 45000, SDS-PAGE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ligand-free enzyme, sitting drop vapor diffusion method, using 0.1 M bis-Tris pH 6.5, 25% (w/v) PEG 3350, 0.2 M lithium sulfate. Enzyme bound to GTP and D-mannose 1-phosphate, sitting drop vapor diffusion method, using 0.1 M magnesium formate, 15% (w/v) PEG 3350
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography, and Superdex 200 gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expression in Agrobacterium tumefaciens
expression in Agrobacterium tumefaciens
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Dowdle, J.; Ishikawa, T.; Gatzek, S.; Rolinski, S.; Smirnoff, N.
Two genes in Arabidopsis thaliana encoding GDP-l-galactose phosphorylase are required for ascorbate biosynthesis and seedling viability
Plant J.
52
673689
2007
Arabidopsis thaliana
Manually annotated by BRENDA team
Siddique, S.; Endres, S.; Atkins, J.M.; Szakasits, D.; Wieczorek, K.; Hofmann, J.; Blaukopf, C.; Urwin, P.E.; Tenhaken, R.; Grundler, F.M.; Kreil, D.P.; Bohlmann, H.
Myo-inositol oxygenase genes are involved in the development of syncytia induced by Heterodera schachtii in Arabidopsis roots
New Phytol.
184
457-472
2009
Arabidopsis thaliana (O22287), Arabidopsis thaliana (Q8H1Q7), Arabidopsis thaliana (Q9M2S0)
Manually annotated by BRENDA team
Barth, C.; Gouzd, Z.A.; Steele, H.P.; Imperio, R.M.
A mutation in GDP-mannose pyrophosphorylase causes conditional hypersensitivity to ammonium, resulting in Arabidopsis root growth inhibition, altered ammonium metabolism, and hormone homeostasis
J. Exp. Bot.
61
379-394
2010
Arabidopsis thaliana
Manually annotated by BRENDA team
Qin, C.; Qian, W.; Wang, W.; Wu, Y.; Yu, C.; Jiang, X.; Wang, D.; Wu, P.
GDP-mannose pyrophosphorylase is a genetic determinant of ammonium sensitivity in Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
105
18308-18313
2008
Arabidopsis thaliana
Manually annotated by BRENDA team
Kempinski, C.F.; Haffar, R.; Barth, C.
Toward the mechanism of NH4+ sensitivity mediated by Arabidopsis GDP-mannose pyrophosphorylase
Plant Cell Environ.
34
847-858
2011
Arabidopsis thaliana, Arabidopsis thaliana Col-0
Manually annotated by BRENDA team
Zhao, S.; Liu, L.
Expression and crystallographic studies of the Arabidopsis thaliana GDP-D-mannose pyrophosphorylase VTC1
Acta Crystallogr. Sect. F
72
795-798
2016
Arabidopsis thaliana (O22287), Arabidopsis thaliana
Manually annotated by BRENDA team