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Information on EC 2.7.7.102 - DNA primase AEP and Organism(s) Saccharomyces cerevisiae and UniProt Accession P10363

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EC Tree
     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.102 DNA primase AEP
IUBMB Comments
The enzyme, which is found in eukaryota and archaea, catalyses the synthesis of short RNA or DNA sequences which are used as primers for EC 2.7.7.7, DNA-directed DNA polymerase.
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Saccharomyces cerevisiae
UNIPROT: P10363
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The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The expected taxonomic range for this enzyme is: Eukaryota, Archaea
Reaction Schemes
+
n
=
ssDNA/pppN(pN)n-1 hybrid
+
(n-1)
+
n
=
ssDNA/pppdN(pdN)n-1 hybrid
+
(n-1)
Synonyms
protein orf904, ccdc111, prislx, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
AEP
-
-
-
-
archaeo-eukaryotic primase
-
-
-
-
PrimPol
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(deoxy)nucleotide 5'-triphosphate:single-stranded DNA (deoxy)nucleotidyltransferase (DNA or DNA-RNA hybrid synthesizing)
The enzyme, which is found in eukaryota and archaea, catalyses the synthesis of short RNA or DNA sequences which are used as primers for EC 2.7.7.7, DNA-directed DNA polymerase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
subunit Pri1
UniProt
Manually annotated by BRENDA team
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D111N
residue is essential for viability
D113N
residue is essential for viability
D314N
residue is essential for viability
H168A
residue is essential for viability
H323A
residue is essential for viability
K326A
residue is essential for viability
R164A
residue is essential for viability
R165A
residue is essential for viability
S162A
residue is essential for viability
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Vaithiyalingam, S.; Arnett, D.R.; Aggarwal, A.; Eichman, B.F.; Fanning, E.; Chazin, W.J.
Insights into eukaryotic primer synthesis from structures of the p48 subunit of human DNA primase
J. Mol. Biol.
426
558-569
2014
Homo sapiens (P49642), Homo sapiens (P49642 and P49643), Saccharomyces cerevisiae (P10363)
Manually annotated by BRENDA team