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Information on EC 2.7.6.2 - thiamine diphosphokinase and Organism(s) Arabidopsis thaliana and UniProt Accession F4IV16

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EC Tree
     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.6 Diphosphotransferases
                2.7.6.2 thiamine diphosphokinase
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: F4IV16 not found.
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
thiamine pyrophosphokinase, pyrophosphokinase, htpk1, thiamin pyrophosphotransferase, thiamine diphosphokinase, thiaminokinase, thiamin:atp pyrophosphotransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
At2g44750 (AtTPK2)
-
thiamin pyrophosphokinase
-
At1g02880 (AtTPK1)
-
-
ATP:thiamin pyrophosphotransferase
-
-
-
-
hTPK1
-
-
-
-
pyrophosphokinase, thiamin
-
-
-
-
thiamin kinase
-
-
-
-
thiamin pyrophosphokinase
thiamin pyrophosphotransferase
-
-
-
-
thiamin:ATP pyrophosphotransferase
-
-
-
-
thiamine kinase
-
-
-
-
thiaminokinase
-
-
-
-
TPTase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diphosphate transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
ATP:thiamine diphosphotransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9026-24-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
thiamin + ATP
thiamindiphosphate + AMP
show the reaction diagram
-
-
-
?
ATP + thiamine
AMP + thiamine diphosphate
show the reaction diagram
thiamin + ATP
thiamindiphosphate + AMP
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
thiamin + ATP
thiamindiphosphate + AMP
show the reaction diagram
-
-
-
?
ATP + thiamine
AMP + thiamine diphosphate
show the reaction diagram
thiamine pyrophosphokinase is required for thiamine cofactor activation in Arabidopsis
-
-
?
thiamin + ATP
thiamindiphosphate + AMP
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00128
thiamine
AtTPK2
0.00096 - 0.00128
thiamine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
AtTPK2 is completely inactive
37 - 50
37°C: optimal activity of TPK2, 50°C: TPK2 is completely inactive
55
AtTPK1 is still active
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TPK2_ARATH
267
0
30214
Swiss-Prot
Secretory Pathway (Reliability: 4)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
inactivation of isozyme TPK2
55
isozyme TPK1 shows remaining activity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
isozymes TPK1 and TPK2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21, expression of fluorescent tagged isozymes in cytosol of mesophyll protoplasts
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ajjawi, I.; Rodriguez Milla, M.A.; Cushman, J.; Shintani, D.K.
Thiamin pyrophosphokinase is required for thiamin cofactor activation in Arabidopsis
Plant Mol. Biol.
65
151-162
2007
Arabidopsis thaliana, Arabidopsis thaliana (F4IV16)
Manually annotated by BRENDA team