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Information on EC 2.7.4.9 - dTMP kinase and Organism(s) Thermus thermophilus and UniProt Accession Q5SHX3

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This record set is specific for:
Thermus thermophilus
UNIPROT: Q5SHX3 not found.
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Word Map
The taxonomic range for the selected organisms is: Thermus thermophilus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
tmpk, dtmp kinase, tmpkmt, thymidine monophosphate kinase, tmp kinase, pftmk, dtmpk, thymidylate monophosphate kinase, s. aureus thymidylate kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
deoxythymidine 5'-monophosphate kinase
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dTMP kinase
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dTMPK
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kinase, thymidine monophosphate (phosphorylating)
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kinase, thymidylate (phosphorylating)
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thymidine 5'-monophosphate kinase
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thymidine monophosphate kinase
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thymidylate kinase
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thymidylate monophosphate kinase
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thymidylic acid kinase
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thymidylic kinase
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TMK
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TMP kinase
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TMPK
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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PATHWAY SOURCE
PATHWAYS
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-, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
ATP:dTMP phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9014-43-1
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + dTMP
ADP + dTDP
show the reaction diagram
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + dTMP
ADP + dTDP
show the reaction diagram
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
important enzyme in DNA synthesis
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 22000, calculated
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
high-resolution X-ray crystal structures of thymidylate kinase in apo and ligand-bound states, structures of binary and ternary complexes of thymidylate kinase with its natural substrates ATP and ATP-TMP, respectively
to 1.83 A resolution, orthorhombic space group P212121, with unit-cell parameters a = 39.50, b = 80.29, c = 122.55 A
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D90L
high similarity to the wild-type. Less than 10% of wild-type activity
K16M
high similarity to the wild-type. Less than 10% of wild-type activity
T18V
equivalent activity to wild-type enzyme, crystal structure of the mutant enzyme is very high similarity to the wild-type structure. Increase in thermal stability by 3.4°C
V158T
equivalent activity to wild-type enzyme, crystal structure of the mutant enzyme is very high similarity to the wild-type structure, thermal stability similar to that of wild-type enzyme
Y162F
Y92H
equivalent activity to wild-type enzyme, crystal structure of the mutant enzyme is very high similarity to the wild-type structure, significant decrease in thermal stability of 8.8 °C as compared with wild-type enzyme
Y99F
high similarity to the wild-type, activity is about 60% of the wild-type activity
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
89.2
Tm-value of wild-type enzyme
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Chaudhary, S.K.; Jeyakanthan, J.; Sekar, K.
Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of thymidylate kinase (TTHA1607) from Thermus thermophilus HB8
Acta Crystallogr. Sect. F
69
118-121
2013
Thermus thermophilus (Q5SHX3), Thermus thermophilus HB8 / ATCC 27634 / DSM 579 (Q5SHX3)
Manually annotated by BRENDA team
Chaudhary, S.K.; Jeyakanthan, J.; Sekar, K.
Structural and functional roles of dynamically correlated residues in thymidylate kinase
Acta Crystallogr. Sect. D
74
341-354
2018
Thermus thermophilus (Q5SHX3), Thermus thermophilus ATCC 27634 (Q5SHX3)
Manually annotated by BRENDA team