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IUBMB CommentsMany nucleoside diphosphates can act as acceptors, while many ribo- and deoxyribonucleoside triphosphates can act as donors.
The taxonomic range for the selected organisms is: Leishmania major
The enzyme appears in selected viruses and cellular organisms
Synonyms
nm23-h1, nucleoside diphosphate kinase, ndp kinase, nm23-h2, ndpk-b, ndpk2, nucleoside diphosphokinase, ndk-1, nucleoside-diphosphate kinase, nucleoside diphosphate kinase a,
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kinase, nucleoside diphosphate (phosphorylating)
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nucleoside 5'-diphosphate kinase
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nucleoside diphosphate (UDP) kinase
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nucleoside diphosphate kinase B
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nucleoside diphosphokinase
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nucleotide phosphate kinase
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uridine diphosphate kinase
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NDK
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phospho group transfer
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-, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -
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ATP:nucleoside-diphosphate phosphotransferase
Many nucleoside diphosphates can act as acceptors, while many ribo- and deoxyribonucleoside triphosphates can act as donors.
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GDP + ATP
GTP + ADP
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ATP + NDP
ADP + NTP
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expression in Escherichia coli
SwissProt
brenda
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Q9U1E1_LEIMA
151
0
16640
TrEMBL
other Location (Reliability: 2)
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18600
x * 18600, SDS-PAGE, x * 18675, mass spectrometry, recombinant protein including 19 amino acid extension
18675
x * 18600, SDS-PAGE, x * 18675, mass spectrometry, recombinant protein including 19 amino acid extension
112000
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estimated from amino acid sequence
18675
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6 * 18675, dynamic light scattering
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x * 18600, SDS-PAGE, x * 18675, mass spectrometry, recombinant protein including 19 amino acid extension
homohexamer
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6 * 18675, dynamic light scattering
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sitting drop vapor diffusion method, using 100 mM citrate-HCl pH 5.6 and 1850 mM ammonium sulfate
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HiTrap chelating column chromatography and Superdex 75 gel filtration
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expression in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
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de Oliveira, A.H.; Ruiz, J.C.; Cruz, A.K.; Greene, L.J.; Rosa, J.C.; Ward, R.J.
Expression in E. coli and purification of the nucleoside diphosphate kinase b from Leishmania major
Protein Expr. Purif.
49
244-250
2006
Leishmania major (Q9U1E1), Leishmania major
brenda
Tonoli, C.C.; Vieira, P.S.; Ward, R.J.; Arni, R.K.; de Oliveira, A.H.; Murakami, M.T.
Production, purification, crystallization and preliminary X-ray diffraction studies of the nucleoside diphosphate kinase b from Leishmania major
Acta Crystallogr. Sect. F
65
1116-1119
2009
Leishmania major
brenda