Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.7.4.6 - nucleoside-diphosphate kinase and Organism(s) Oryza sativa and UniProt Accession Q07661

for references in articles please use BRENDA:EC2.7.4.6
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
Many nucleoside diphosphates can act as acceptors, while many ribo- and deoxyribonucleoside triphosphates can act as donors.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Oryza sativa
UNIPROT: Q07661
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Oryza sativa
The enzyme appears in selected viruses and cellular organisms
Synonyms
nm23-h1, nucleoside diphosphate kinase, ndp kinase, nm23-h2, ndpk-b, ndpk2, nucleoside diphosphokinase, ndk-1, nucleoside-diphosphate kinase, nucleoside diphosphate kinase a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
kinase, nucleoside diphosphate (phosphorylating)
-
-
-
-
NDK
-
-
-
-
NDPK1
-
isozyme
NDPK2
-
isozyme
NDPK3
-
isozyme
nucleoside 5'-diphosphate kinase
-
-
-
-
nucleoside diphosphate (UDP) kinase
-
-
-
-
nucleoside diphosphate kinase
-
-
nucleoside diphosphokinase
-
-
-
-
nucleotide phosphate kinase
-
-
-
-
OsNDPK2
-
-
UDP kinase
-
-
-
-
uridine diphosphate kinase
-
-
-
-
WSL12
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:nucleoside-diphosphate phosphotransferase
Many nucleoside diphosphates can act as acceptors, while many ribo- and deoxyribonucleoside triphosphates can act as donors.
CAS REGISTRY NUMBER
COMMENTARY hide
9026-51-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + dNDP
ADP + dNTP
show the reaction diagram
contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
ADP + CTP
ATP + CDP
show the reaction diagram
-
-
-
-
?
ADP + GTP
ATP + GDP
show the reaction diagram
-
-
-
-
?
ADP + TTP
ATP + TDP
show the reaction diagram
-
-
-
-
?
NTP + nucleoside diphosphate
NDP + NTP
show the reaction diagram
-
-
-
-
r
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + dNDP
ADP + dNTP
show the reaction diagram
contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates
-
-
?
ATP + NDP
ADP + NTP
show the reaction diagram
contributes to the maintenance of the cellular pools of all nucleoside triphosphates, involved in coleoptile elongation and anaerobic stress response
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.079 - 1.26
ADP
5.28 - 9.37
CTP
0.45 - 1.69
GTP
0.89 - 5.28
TTP
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
85.2 - 229
CTP
17.9 - 49.4
GTP
14.2 - 55
TTP
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
67.6 - 415
ADP
9.1 - 34.1
CTP
29.2 - 61.6
GTP
10.4 - 25
TTP
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2
-
isozymes NDPK2 and NDPK3
8.4
-
isozyme NDPK1
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
WSL12 is highly expressed in young tissues
Manually annotated by BRENDA team
-
WSL12 is highly expressed in young tissues
Manually annotated by BRENDA team
-
immature seed
Manually annotated by BRENDA team
additional information
-
isoform NDPK1 is weakly expressed in leaf sheaths
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
isozyme NDPK1
Manually annotated by BRENDA team
-
isozyme NDPK3
Manually annotated by BRENDA team
-
isozyme NDPK2
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
WSL12 plays an important role in chloroplast development and chlorophyll biosynthesis by regulating the expression levels of related genes. In addition, WSL12 also affects the response to abiotic stress, such as abscisic acid and salinity in rice, and is beneficial to molecular breeding of stress tolerance
PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
125000
gel filtration
17000
6 * 17000, crystal structure analysis
17400
-
x * 17400, isozyme NDPK2, calculated from amino acid sequence
17900
-
x * 17900, isozyme NDPK1, calculated from amino acid sequence
18000
-
x * 18000, isozyme NDPK3, calculated from amino acid sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
6 * 17000, crystal structure analysis
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapor-diffusion method
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
transgenic plants bearing gene for enzyme antisense RNA, inhibition of cell elongation process
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 9
-
isozyme NDPK3
721870
6 - 9
-
isozyme NDPK2
721870
7 - 9
-
isozyme NDPK1
721870
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 60
-
after 15 min incubation at 40°C, isozymes NDPK1 and NDPK3 show about 40% and 80% residual activity and no activity at 60°C, respectively, while isozyme NDPK2 shows about 40% residual activity at 60°C
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant protein
Ni-chelating Sepharose column chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as HAT-tag fusion protein in Escherichia coli
expressed in Escherichia coli BL21(DE3)pLyS cells
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the expression levels of WSL12 are higher in plants grown at 22°C and 34°C than those grown at 26°C and 30°C
-
WSL12 is highly expressed in young tissues
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Pan, L.; Kawai, M.; Yano, A.; Uchimiya, H.
Nucleoside diphosphate kinase required for coleoptile elongation in rice
Plant Physiol.
122
447-452
2000
Oryza sativa
Manually annotated by BRENDA team
Huang, J.Y.; Chang, T.; Chang, C.Y.; Chen, C.J.
Crystal structure of nucleoside diphosphate kinase required for coleoptile elongation in rice (Oryza sativa L.)
J. Struct. Biol.
150
309-318
2005
Oryza sativa (Q07661), Oryza sativa
Manually annotated by BRENDA team
Kihara, A.; Saburi, W.; Wakuta, S.; Kim, M.H.; Hamada, S.; Ito, H.; Imai, R.; Matsui, H.
Physiological and biochemical characterization of three nucleoside diphosphate kinase isozymes from rice (Oryza sativa L.)
Biosci. Biotechnol. Biochem.
75
1740-1745
2011
Oryza sativa
Manually annotated by BRENDA team
Ye, W.; Hu, S.; Wu, L.; Ge, C.; Cui, Y.; Chen, P.; Wang, X.; Xu, J.; Ren, D.; Dong, G.; Qian, Q.; Guo, L.
White stripe leaf 12 (WSL12), encoding a nucleoside diphosphate kinase 2 (OsNDPK2), regulates chloroplast development and abiotic stress response in rice (Oryza sativa L.)
Mol. Breed.
36
57
2016
Oryza sativa
Manually annotated by BRENDA team