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EC Tree
IUBMB Comments Many nucleoside diphosphates can act as acceptors, while many ribo- and deoxyribonucleoside triphosphates can act as donors.
The taxonomic range for the selected organisms is: Oryza sativa The enzyme appears in selected viruses and cellular organisms
Synonyms
nm23-h1, nucleoside diphosphate kinase, ndp kinase, nm23-h2, ndpk-b, ndpk2, nucleoside diphosphokinase, ndk-1, nucleoside-diphosphate kinase, nucleoside diphosphate kinase a,
more
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kinase, nucleoside diphosphate (phosphorylating)
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nucleoside 5'-diphosphate kinase
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nucleoside diphosphate (UDP) kinase
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nucleoside diphosphate kinase
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nucleoside diphosphokinase
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nucleotide phosphate kinase
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uridine diphosphate kinase
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phospho group transfer
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-, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -
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ATP:nucleoside-diphosphate phosphotransferase
Many nucleoside diphosphates can act as acceptors, while many ribo- and deoxyribonucleoside triphosphates can act as donors.
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ATP + dNDP
ADP + dNTP
contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates
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ADP + CTP
ATP + CDP
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ADP + GTP
ATP + GDP
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ADP + TTP
ATP + TDP
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NTP + nucleoside diphosphate
NDP + NTP
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r
ATP + NDP
ADP + NTP
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?
ATP + NDP
ADP + NTP
contributes to the maintenance of the cellular pools of all nucleoside triphosphates, involved in coleoptile elongation and anaerobic stress response
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ATP + dNDP
ADP + dNTP
contributes to the maintenance of the cellular pools of all deoxynucleoside triphosphates
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ATP + NDP
ADP + NTP
contributes to the maintenance of the cellular pools of all nucleoside triphosphates, involved in coleoptile elongation and anaerobic stress response
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0.079
ADP
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isozyme NDPK2, with TTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
0.108
ADP
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isozyme NDPK3, with TTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
0.125
ADP
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isozyme NDPK3, with GTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
0.138
ADP
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isozyme NDPK2, with GTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
0.392
ADP
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isozyme NDPK1, with TTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
0.552
ADP
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isozyme NDPK1, with CTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
0.578
ADP
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isozyme NDPK3, with CTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
0.707
ADP
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isozyme NDPK1, with GTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
1.26
ADP
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isozyme NDPK2, with CTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
5.28
CTP
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isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
6.72
CTP
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isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
9.37
CTP
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isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
0.45
GTP
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isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
0.51
GTP
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isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
1.69
GTP
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isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
0.89
TTP
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isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
1.34
TTP
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isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
5.28
TTP
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isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
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85.2
CTP
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isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
89
CTP
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isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
229
CTP
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isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
17.9
GTP
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isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
27.7
GTP
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isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
49.4
GTP
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isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
14.2
TTP
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isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
33.5
TTP
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isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
55
TTP
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isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
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67.6
ADP
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isozyme NDPK2, with CTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
69.9
ADP
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isozyme NDPK1, with GTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
140
ADP
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isozyme NDPK1, with TTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
143
ADP
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isozyme NDPK3, with GTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
154
ADP
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isozyme NDPK3, with CTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
180
ADP
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isozyme NDPK2, with TTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
210
ADP
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isozyme NDPK2, with GTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
310
ADP
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isozyme NDPK3, with TTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
415
ADP
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isozyme NDPK1, with CTP as cosubstrate, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
9.1
CTP
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isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
16.9
CTP
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isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
34.1
CTP
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isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
29.2
GTP
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isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
35.1
GTP
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isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
61.6
GTP
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isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
10.4
TTP
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isozyme NDPK1, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
16
TTP
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isozyme NDPK2, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
25
TTP
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isozyme NDPK3, in 4 mM Tris-HCl, pH 7.5, 100 mM KCl, 25 mM MgCl2, at 25°C
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6.2
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isozymes NDPK2 and NDPK3
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Uniprot
brenda
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WSL12 is highly expressed in young tissues
brenda
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WSL12 is highly expressed in young tissues
brenda
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immature seed
brenda
additional information
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isoform NDPK1 is weakly expressed in leaf sheaths
brenda
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brenda
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highly expressed in leaves and young tissues
brenda
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brenda
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WSL12 is highly expressed in young tissues
brenda
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brenda
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isozyme NDPK1
brenda
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isozyme NDPK3
brenda
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isozyme NDPK2
brenda
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physiological function
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WSL12 plays an important role in chloroplast development and chlorophyll biosynthesis by regulating the expression levels of related genes. In addition, WSL12 also affects the response to abiotic stress, such as abscisic acid and salinity in rice, and is beneficial to molecular breeding of stress tolerance
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17000
6 * 17000, crystal structure analysis
17400
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x * 17400, isozyme NDPK2, calculated from amino acid sequence
17900
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x * 17900, isozyme NDPK1, calculated from amino acid sequence
18000
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x * 18000, isozyme NDPK3, calculated from amino acid sequence
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hexamer
6 * 17000, crystal structure analysis
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x * 17400, isozyme NDPK2, calculated from amino acid sequence
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x * 17900, isozyme NDPK1, calculated from amino acid sequence
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x * 18000, isozyme NDPK3, calculated from amino acid sequence
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hanging-drop vapor-diffusion method
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additional information
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transgenic plants bearing gene for enzyme antisense RNA, inhibition of cell elongation process
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4 - 9
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isozyme NDPK3
721870
6 - 9
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isozyme NDPK2
721870
7 - 9
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isozyme NDPK1
721870
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40 - 60
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after 15 min incubation at 40°C, isozymes NDPK1 and NDPK3 show about 40% and 80% residual activity and no activity at 60°C, respectively, while isozyme NDPK2 shows about 40% residual activity at 60°C
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Ni-chelating Sepharose column chromatography
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expressed as HAT-tag fusion protein in Escherichia coli
expressed in Escherichia coli BL21(DE3)pLyS cells
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the expression levels of WSL12 are higher in plants grown at 22°C and 34°C than those grown at 26°C and 30°C
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WSL12 is highly expressed in young tissues
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Pan, L.; Kawai, M.; Yano, A.; Uchimiya, H.
Nucleoside diphosphate kinase required for coleoptile elongation in rice
Plant Physiol.
122
447-452
2000
Oryza sativa
brenda
Huang, J.Y.; Chang, T.; Chang, C.Y.; Chen, C.J.
Crystal structure of nucleoside diphosphate kinase required for coleoptile elongation in rice (Oryza sativa L.)
J. Struct. Biol.
150
309-318
2005
Oryza sativa (Q07661), Oryza sativa
brenda
Kihara, A.; Saburi, W.; Wakuta, S.; Kim, M.H.; Hamada, S.; Ito, H.; Imai, R.; Matsui, H.
Physiological and biochemical characterization of three nucleoside diphosphate kinase isozymes from rice (Oryza sativa L.)
Biosci. Biotechnol. Biochem.
75
1740-1745
2011
Oryza sativa
brenda
Ye, W.; Hu, S.; Wu, L.; Ge, C.; Cui, Y.; Chen, P.; Wang, X.; Xu, J.; Ren, D.; Dong, G.; Qian, Q.; Guo, L.
White stripe leaf 12 (WSL12), encoding a nucleoside diphosphate kinase 2 (OsNDPK2), regulates chloroplast development and abiotic stress response in rice (Oryza sativa L.)
Mol. Breed.
36
57
2016
Oryza sativa
brenda