Any feedback?
Information on EC 2.7.4.6 - nucleoside-diphosphate kinase and Organism(s) Arabidopsis thaliana and UniProt Accession O64903
for references in articles please use BRENDA:EC2.7.4.6Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
2.7.4.6 nucleoside-diphosphate kinaseIUBMB Comments
Many nucleoside diphosphates can act as acceptors, while many ribo- and deoxyribonucleoside triphosphates can act as donors.
Specify your search results
Word Map
- 2.7.4.6
- triphosphate
- node
- gdp
- lymph
- adenylate
- metastases
- tumour
- autophosphorylation
- colorectal
- oncogene
- drosophila
- guanine
- c-myc
-
clinicopathological
- melanoma
- creatine
- dictyostelium
-
hexameric
-
gtp-binding
- discoideum
-
non-metastatic
-
gamma-phosphate
-
anti-metastatic
-
heterotrimeric
-
phosphohistidine
-
transphosphorylation
- dntp
- deoxyribonucleoside
- succinyl-coa
- phosphoenzyme
-
metastasis-related
- mastoparan
-
high-metastatic
-
metastasis-associated
-
prune
-
c-erbb-2
- myxococcus
-
g-quadruplexes
- deoxynucleoside
- xanthus
- medicine
- drug development
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Synonyms
nm23-h1, nucleoside diphosphate kinase, ndp kinase, nm23-h2, ndpk-b, ndpk2, nucleoside diphosphokinase, ndk-1, nucleoside-diphosphate kinase, nucleoside diphosphate kinase a, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
kinase, nucleoside diphosphate (phosphorylating)
-
-
-
-
NDK
-
-
-
-
NDPK2
nucleoside 5'-diphosphate kinase
-
-
-
-
nucleoside diphosphate (UDP) kinase
-
-
-
-
nucleoside diphosphate kinase
nucleoside diphosphokinase
-
-
-
-
nucleotide phosphate kinase
-
-
-
-
UDP kinase
-
-
-
-
uridine diphosphate kinase
-
-
-
-
additional information
additional information
-
the enzyme belongs to the nucleoside diphosphate kinase family
additional information
-
the enzyme belongs to the nucleoside diphosphate kinase gene family
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
KEGG
-
-, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
ATP:nucleoside-diphosphate phosphotransferase
Many nucleoside diphosphates can act as acceptors, while many ribo- and deoxyribonucleoside triphosphates can act as donors.
CAS REGISTRY NUMBER
COMMENTARY
9026-51-1
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
NDPK is involved in multiple signaling pathways in mammalian systems, including G-protein signaling. Physical interaction between NDPK2 and small G proteins, as well as the heterotrimeric G protein GPA1. NDPK2 can stimulate their GTPase activities, but the dimeric form of NDPK2 is also unable to enhance the GTPase activities of both Pra2 and Pra3, the role oligomeric states may play in the interaction of NDPK2 with small G proteins
-
-
?
additional information
?
-
NDPK is involved in multiple signaling pathways in mammalian systems, including G-protein signaling. Physical interaction between NDPK2 and small G proteins, as well as the heterotrimeric G protein GPA1. NDPK2 can stimulate their GTPase activities, but the dimeric form of NDPK2 is also unable to enhance the GTPase activities of both Pra2 and Pra3, the role oligomeric states may play in the interaction of NDPK2 with small G proteins
-
-
?
additional information
?
-
nucleoside diphosphate kinase is a housekeeping enzyme that maintains the intracellular levels of all (d)NTPs used in biosynthesis except ATP, but it also plays a significant role in signal transduction pathways involved in oxidative stress. The enzyme protects the plants against reactive ocygen species, heat, and salt stress, overview
-
-
?
additional information
?
-
-
NDPK2 interacts with plant small G proteins in vitro, both Pra2 and Pra3both Pra2 and Pra3, the role oligomeric states may play in the interaction of NDPK2 with small G proteins
-
-
?
additional information
?
-
NDPK2 interacts with plant small G proteins in vitro, both Pra2 and Pra3both Pra2 and Pra3, the role oligomeric states may play in the interaction of NDPK2 with small G proteins
-
-
?
additional information
?
-
-
NDPK2 is a H2O2 signaling protein that performs autophosphorylation. NDPK2 and H2O2 are involved in salt resistance, overview
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
NDPK is involved in multiple signaling pathways in mammalian systems, including G-protein signaling. Physical interaction between NDPK2 and small G proteins, as well as the heterotrimeric G protein GPA1. NDPK2 can stimulate their GTPase activities, but the dimeric form of NDPK2 is also unable to enhance the GTPase activities of both Pra2 and Pra3, the role oligomeric states may play in the interaction of NDPK2 with small G proteins
-
-
?
additional information
?
-
NDPK is involved in multiple signaling pathways in mammalian systems, including G-protein signaling. Physical interaction between NDPK2 and small G proteins, as well as the heterotrimeric G protein GPA1. NDPK2 can stimulate their GTPase activities, but the dimeric form of NDPK2 is also unable to enhance the GTPase activities of both Pra2 and Pra3, the role oligomeric states may play in the interaction of NDPK2 with small G proteins
-
-
?
additional information
?
-
nucleoside diphosphate kinase is a housekeeping enzyme that maintains the intracellular levels of all (d)NTPs used in biosynthesis except ATP, but it also plays a significant role in signal transduction pathways involved in oxidative stress. The enzyme protects the plants against reactive ocygen species, heat, and salt stress, overview
-
-
?
additional information
?
-
-
NDPK2 is a H2O2 signaling protein that performs autophosphorylation. NDPK2 and H2O2 are involved in salt resistance, overview
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
cAMP
decreases the reaction speed of NDPK1 proportionally to the ligand concentration. Addition of 0.1 mM, 0.2 mM, and 0.5 mM cAMP reduces the specific activity of NDPK1 to 96%, 91%, and 84%, respectively, of the control
cGMP
decreases the reaction speed of NDPK1 proportionally to the ligand concentration. Addition of 0.1 mM, 0.2 mM, and 0.5 mM cGMP reduces the reaction speed to 84%, 69%, and 51% of the control
additional information
-
SOS2, a class 3 sucrose-nonfermenting 1-related kinase, interacts with the H2O2 signaling protein NDPK2 and inhibits its autophosphorylation activity
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
isozyme NDPK3a is highly inducible by glucose and sucrose, other NDPK isozymes are less sensitive to sucrose, overview
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
12.1
pH and temperature not specified in the publication, NDPK1
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
maximum levels of NDPK1 expression are found in procambium cells while expression of genes for other isoforms are either absent
additional information
-
NDPK1 is the most highly expressed isozyme, followed by isozymes NDPK3a and NDPK3b, the latter is generally expressed at very low extent but increased in inflorescences and dividing cells
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
NDPK3 is localized to chloroplast
additional information
-
the NDPK isozymes are localized in different subcelllar compartments, distribution, overview
-
-
isozyme NDPK2 is localized in the stroma, no NDPK3 in the chloroplasts
NDPK2 is exclusively targeted to chloroplasts
predominant localization of NDPK1 in cytosol
-
isozyme NDPK3a is predominantly localized in the mictochondria
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
key enzyme regulating NDP/NTP homeostasis
metabolism
proteomic analysis in wild-type and knockout mutant plants under normal or phenanthrene stress conditions, genes expression analysis, overview
physiological function
physiological function
Arabidopsis thaliana nucleoside diphosphate kinase, NDPK-3, is involved in stress signaling in response to polycyclic aromatic hydrocarbon exposure. Isozyme NDPK3 is a positive regulator in the Arabidopsis response to phenanthrene stress
physiological function
key enzyme regulating NDP/NTP homeostasis
physiological function
the enzyme plays a key role in the transfer of energy between the cytosolic adenine and uridine nucleotide pools and in the distribution of carbon between starch and cellulose
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). NDK2_ARATH
231
0
25550
Swiss-Prot
Chloroplast (Reliability: 1)
PDB
SCOP
CATH
UNIPROT
ORGANISM
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glutathionylation
NDPK1 undergoes glutathionylation at Cys43
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
H197C
no enzymatic activity, stimulates GTPase activites of Pra2 and Pra3, EC 3.6.5.2, in the same manner as wild-type NDPK2
L225stop
increased enzymatic activity, stimulates GTPase activites of Pra2 and Pra3, EC 3.6.5.2, in the same manner as wild-type NDPK2
LP175S
no interaction between NDPK2 and small G proteins, EC 3.6.5.2
P175S
stimulates GTPase activites of Pra2 and Pra3, EC 3.6.5.2, with a slight reduction compared to wild-type NDPK2
R230stop
increased enzymatic activity, stimulates GTPase activites of Pra2 and Pra3, EC 3.6.5.2, in the same manner as wild-type NDPK2
additional information
additional information
transgenic Solanum tubersoum plants overexpressing the Arabidopsis thaliana NDPK2 isozyme show reduced sensitivity to stress caused by methyl viologen, i.e. reactive oxigen species, or salt or heat, compared to the wild-type plants, overview
additional information
-
a ndpk2 knockout mutant shows increased salt stress sensitivity compared to the wild-type enzyme. The sos2-2/ndpk2 double mutant is more salt sensitive than a sos2-2 single mutant, and does not hyperaccumulate H2O2 in response to salt stress
additional information
-
NDPK3a induction by sugars is reduced in AtWrky4 and AtWrky34 knockout mutants, Wrky4 and Wrky34 are transcription factor proteins involved in sugar induction and response, overview
additional information
construction of NDPK3 knockout and overexpression lines: in the NDPK-3 overexpressor, the enzyme activities of ascorbate peroxidase, peroxiredoxin, catalase, as well as superoxide dismutase are all increased compared to wild-type, in the NDPK-3 knockout line, these enzymes have reduced activity compared to wild-type. Proteomic analysis of mutants, overview
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
60.3
Tm-value, nucleoside diphosphate kinase NDPK1, in water
60.4
Tm-value, nucleoside diphosphate kinase NDPK1, 1 mM IMP, in water
60.9
Tm-value, nucleoside diphosphate kinase NDPK1, 1 mM adenylosuccinate, in water
62.6
Tm-value, nucleoside diphosphate kinase NDPK1, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol
62.7
62.8
Tm-value, nucleoside diphosphate kinase NDPK1, addition of 0.1 mM cGMP, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol
63.5
Tm-value, nucleoside diphosphate kinase NDPK1, addition of 1 mM cAMP, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol
64.4
Tm-value, nucleoside diphosphate kinase NDPK1, addition of 1 mM cGMP, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol
additional information
62.7
Tm-value, nucleoside diphosphate kinase NDPK1, 0.01 mM ATP, in water
62.7
Tm-value, nucleoside diphosphate kinase NDPK1, addition of 0.1 mM cAMP, in 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 20% glycerol
additional information
3',5'-cAMP and 3',5'-cGMP at 1 mM stabilize NDPK1, shifting the Tm by 0.9-1.8°C
additional information
3',5'-cAMP and 3',5'-cGMP at 1 mM stabilize NDPK1, shifting the Tm by 0.9-1.8°C
additional information
3',5'-cAMP and 3',5'-cGMP at 1 mM stabilize NDPK1, shifting the Tm by 0.9-1.8°C
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
ATP stabilizes the enzyme at a concentration of 0.01 mM
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant GST-tagged wild-type enzyme from Escherichia coli by glutathione affinity chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned into plant binary vector in which the constitutive 35S promoter is driving the expression of either C- or N-terminally tagged enzyme
expression of isozyme NDPK2 in Solanum tuberosum cv. Atlantic plant leaf cytosol under the control of an oxidative stress-inducible SWPA2 promoter or enhanced CaMV 35S promoter, resulting in transgenic lines SN1, SN19 and EN1, EN2
cloned into plant binary vector in which the constitutive 35S promoter is driving the expression of either C- or N-terminally tagged enzyme
cloned into plant binary vector in which the constitutive 35S promoter is driving the expression of either C- or N-terminally tagged enzyme (NDPK)
gene ndpk2, expression of GST-tagged wild-type enzyme in Escherichia coli, expression of SOS2 and NDPK2 in Saccharomyces cerevisiae in the two-hybrid system
-
gene NDPK3 or At4g11010, enzyme overexpression in transgenic Arabidopsis thalina a lines, quantitative RT-PCR expression analysis
gene NDPK3a, expression analysis, NDPK3a transcript abundance in leaves treated with different sugars and NAG, overview
-
NDPK isozymes, sequence comparisons and phylogenetic analysis, expression of GFP-tagged isozyme NDPK3 in Arabidopsis thaliana protoplasts, targeting to the mitochondria
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Shen, Y.; Han, Y.J.; Kim, J.I.; Song, P.S.
Arabidopsis nucleoside diphosphate kinase-2 as a plant GTPase activating protein
BMB Rep.
41
645-650
2008
Arabidopsis thaliana, Arabidopsis thaliana (O64903)
Verslues, P.E.; Batelli, G.; Grillo, S.; Agius, F.; Kim, Y.S.; Zhu, J.; Agarwal, M.; Katiyar-Agarwal, S.; Zhu, J.K.
Interaction of SOS2 with nucleoside diphosphate kinase 2 and catalases reveals a point of connection between salt stress and H2O2 signaling in Arabidopsis thaliana
Mol. Cell. Biol.
27
7771-7780
2007
Arabidopsis thaliana
Hammargren, J.; Sundstroem, J.; Johansson, M.; Bergman, P.; Knorpp, C.
On the phylogeny, expression and targeting of plant nucleoside diphosphate kinases
Physiol. Plant.
129
79-89
2007
Arabidopsis thaliana, Pisum sativum (P47922), Pisum sativum (P47923)
Hammargren, J.; Rosenquist, S.; Jansson, C.; Knorpp, C.
A novel connection between nucleotide and carbohydrate metabolism in mitochondria: sugar regulation of the Arabidopsis nucleoside diphosphate kinase 3a gene
Plant Cell Rep.
27
529-534
2008
Arabidopsis thaliana
Tang, L.; Kim, M.D.; Yang, K.S.; Kwon, S.Y.; Kim, S.H.; Kim, J.S.; Yun, D.J.; Kwak, S.S.; Lee, H.S.
Enhanced tolerance of transgenic potato plants overexpressing nucleoside diphosphate kinase 2 against multiple environmental stresses
Transgenic Res.
17
705-715
2008
Arabidopsis thaliana (O64903)
Liu, H.; Weisman, D.; Tang, L.; Tan, L.; Zhang, W.; Wang, Z.; Huang, Y.; Lin, W.; Liu, X.; Colon-Carmona, A.
Stress signaling in response to polycyclic aromatic hydrocarbon exposure in Arabidopsis thaliana involves a nucleoside diphosphate kinase, NDPK-3
Planta
241
95-107
2014
Arabidopsis thaliana (O49203), Arabidopsis thaliana Col-0 (O49203)
-
Luzarowski, M.; Kosmacz, M.; Sokolowska, E.; Jasinska, W.; Willmitzer, L.; Veyel, D.; Skirycz, A.
Affinity purification with metabolomic and proteomic analysis unravels diverse roles of nucleoside diphosphate kinases
J. Exp. Bot.
68
3487-3499
2017
Arabidopsis thaliana (O49203), Arabidopsis thaliana (O64903), Arabidopsis thaliana (P39207)
EXTERNAL LINKS (specific for EC-Number 2.7.4.6)
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
