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IUBMB Comments Many nucleotides can act as acceptors; other nucleoside triphosphates can act instead of ATP.
The enzyme appears in viruses and cellular organisms
Synonyms nucleoside monophosphate kinase, ampk1, nmp kinase, ampk2, more
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kinase, nucleoside monophosphate (phosphorylating)
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nucleoside monophosphate kinase
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ATP + nucleoside phosphate = ADP + nucleoside diphosphate
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phospho group transfer
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ATP:nucleoside-phosphate phosphotransferase
Many nucleotides can act as acceptors; other nucleoside triphosphates can act instead of ATP.
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ATP + dAMP
ADP + dADP
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Substrates: 100% activity Products: -
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ATP + GMP
ADP + GDP
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Substrates: GTP, ITP, UTP are not donor substrates, IMP is not acceptor substrate Products: -
r
ATP + TMP
ADP + TDP
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Substrates: - Products: -
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ATP + UMP
ADP + UDP
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Substrates: - Products: -
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ATP + [2-[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]ethyl]boronic acid
ADP + (2-((2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl)ethyl)boronic phosphoric anhydride
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Substrates: - Products: -
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GTP + AMP
GDP + ADP
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Substrates: GMP, CMP, UMP or IMP are not acceptor substrates Products: -
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ITP + AMP
IDP + ADP
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Substrates: IMP or GMP are not acceptor substrates Products: -
r
additional information
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additional information
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Substrates: no activity with 3-(thymin-1-yl)propylboronic acid, (E)-3-(thymin-1-yl)prop-1-enylboronic acid, and (E)-1-(5'-deoxy-thymidin-5'-yl)-vinyl boronic acid Products: -
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additional information
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Substrates: the enzyme shows almost no activity towards tenofovir and adefovir Products: -
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ATP + TMP
ADP + TDP
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Substrates: - Products: -
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Ca2+
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half as effective as Mg2+ at 4 mM
Mn2+
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as effective as Mg2+
Mg2+
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activity saturation at 4 mM
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(E)-1-(5'-deoxy-thymidin-5'-yl)-vinyl boronic acid
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about 30% inhibition at 1 mM
3-(thymin-1-yl)propylboronic acid
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about 10% inhibition at 1 mM
[(E)-2-[(2R,3S,5R)-5-(6-amino-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl]ethenyl]boronic acid
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about 15% inhibition at 1 mM
[2-[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]ethyl]boronic acid
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about 45% inhibition at 1 mM
[2-[(2R,3S,5R)-5-(2-amino-6-oxo-1,6-dihydro-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl]ethyl]boronic acid
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about 25% inhibition at 1 mM
[2-[(2R,3S,5R)-5-(4-amino-2-oxopyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl]ethyl]boronic acid
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about 55% inhibition at 1 mM
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EDTA
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variable degree of activation
glutathione
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variable degree of activation
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Lymphoma, T-Cell
Roles of GTP and Rho GTPases in pancreatic islet beta cell function and dysfunction.
Neoplasms
Cytidine monophosphate kinase is inhibited by the TGF-? signalling pathway through the upregulation of miR-130b-3p in human epithelial ovarian cancer.
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0.23
TMP
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in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37°C
0.119
[2-[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]ethyl]boronic acid
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in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37°C
0.08
dAMP
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isoform AMPK2, in 50 mM Tris/HCl (pH 7.4), 50 mM KCl, 5 mM MgCl2, at 37°C
0.14
dAMP
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isoform AMPK1, in 50 mM Tris/HCl (pH 7.4), 50 mM KCl, 5 mM MgCl2, at 37°C
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1.25
TMP
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in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37°C
0.014
[2-[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]ethyl]boronic acid
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in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37°C
80
dAMP
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isoform AMPK2, in 50 mM Tris/HCl (pH 7.4), 50 mM KCl, 5 mM MgCl2, at 37°C
500
dAMP
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isoform AMPK1, in 50 mM Tris/HCl (pH 7.4), 50 mM KCl, 5 mM MgCl2, at 37°C
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5400
TMP
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in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37°C
0.139
[2-[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]ethyl]boronic acid
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in Tris-HCl (50 mM, pH 7.4), KCl (50 mM), MgCl2 (5 mM), at 37°C
1000
dAMP
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isoform AMPK2, in 50 mM Tris/HCl (pH 7.4), 50 mM KCl, 5 mM MgCl2, at 37°C
3000
dAMP
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isoform AMPK1, in 50 mM Tris/HCl (pH 7.4), 50 mM KCl, 5 mM MgCl2, at 37°C
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additional information
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description of assay method
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6.5 - 7.5
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10% of the activity at pH 5.3
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brenda
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brenda
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brenda
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brenda
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brenda
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Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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KAD9_HUMAN
1911
0
221413
Swiss-Prot
other Location (Reliability: 1 )
PYRH_ECOLI
Escherichia coli (strain K12)
241
0
25970
Swiss-Prot
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1.3
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inactivation in few min at 2°C
642629
7
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60°C, stable for 2 min
642628
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60
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2 min, considerably stable at pH 7
80
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completely inactivated at pH 7
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-15°C, 3 weeks, 75% of its activity lost
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frozen, crude enzyme solution, indefinitely
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partial, using ammonium sulfate precipitation
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Ayengar, P.; Gibson, D.M.; Sanadi, D.R.
Transphosphorylations between nucleoside phosphates
Biochim. Biophys. Acta
21
86-91
1956
Sus scrofa
brenda
Strominger, J.L.; Heppel, L.A.; Maxwell, E.S.
Nucleoside monophosphate kinases. Transphosphorilation between adenosine triphosphate and nucleoside monophosphates
Biochim. Biophys. Acta
32
412-421
1959
Bos taurus
brenda
El Amri, C.; Martin, A.; Vasseur, J.; Smietana, M.
Borononucleotides as substrates/binders for human NMP kinases: Enzymatic and spectroscopic evaluation
ChemBioChem
13
1605-1612
2012
Homo sapiens
brenda
Gallier, F.; Alexandre, J.A.; El Amri, C.; Deville-Bonne, D.; Peyrottes, S.; Perigaud, C.
5,6-nucleoside phosphonate analogues architecture: synthesis and comparative evaluation towards metabolic enzymes
ChemMedChem
6
1094-1106
2011
Homo sapiens
brenda
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