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IUBMB CommentsRequires Mg2+. The enzyme, isolated from the archaeon Methanocaldococcus jannaschii, participates in the biosynthesis of the methanofuran cofactor.
The expected taxonomic range for this enzyme is: Archaea, Bacteria
Synonyms
MJ0458,
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MJ0458

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ATP + [5-(aminomethyl)furan-3-yl]methyl phosphate = ADP + [5-(aminomethyl)furan-3-yl]methyl diphosphate
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ATP:[5-(aminomethyl)furan-3-yl]methyl-phosphate phosphotransferase
Requires Mg2+. The enzyme, isolated from the archaeon Methanocaldococcus jannaschii, participates in the biosynthesis of the methanofuran cofactor.
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ATP + [5-(aminomethyl)furan-3-yl]methyl phosphate
ADP + [5-(aminomethyl)furan-3-yl]methyl diphosphate
AMP + ATP

2 ADP
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AMP + ATP
2 ADP
reaction of adenylate kinase, EC 2.7.4.3
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AMP + ATP
2 ADP
reaction of adenylate kinase, EC 2.7.4.3
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ATP + [5-(aminomethyl)furan-3-yl]methyl phosphate

ADP + [5-(aminomethyl)furan-3-yl]methyl diphosphate
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ATP + [5-(aminomethyl)furan-3-yl]methyl phosphate
ADP + [5-(aminomethyl)furan-3-yl]methyl diphosphate
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Mg2+
MfnE exclusively uses Mg2+ as a cofactor to facilitate phosphoryl group transfer
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EDTA
10 mM, complete loss of activity
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0.072
substrate AMP, pH 7.0, 80°C
0.16
substrate 5-(aminomethyl)-3-furanmethanol-phosphate, pH 7.0, 80°C
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SwissProt
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SwissProt
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physiological function

enzyme catalyzes the formation of methanofuran precursor 5-(aminomethyl)-3-furanmethanol-diphosphate (F1-PP) from ATP
physiological function
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enzyme catalyzes the formation of methanofuran precursor 5-(aminomethyl)-3-furanmethanol-diphosphate (F1-PP) from ATP
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MFNE_METJA
Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)
216
0
24080
Swiss-Prot
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A0A7G9YYN5_9EURY
237
0
26151
TrEMBL
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A0A7G9Z9I6_9EURY
211
0
23429
TrEMBL
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A0A822J0H2_9EURY
199
0
21799
TrEMBL
-
A0A811T2K5_9EURY
204
0
22356
TrEMBL
-
A0A7R9MXI5_9EURY
204
0
21671
TrEMBL
-
A0A5E4KRB1_9ARCH
153
0
17119
TrEMBL
-
A0A5E4L2R8_9ARCH
199
0
21972
TrEMBL
-
A0A8B3RY49_9EURY
204
0
22200
TrEMBL
-
A0A5E4K933_9ARCH
199
0
21772
TrEMBL
-
A0A811TGV3_9EURY
204
0
22305
TrEMBL
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A0A7R6WAL1_9GAMM
Methyloprofundus sp
194
0
21041
TrEMBL
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A0A822IS45_9EURY
199
0
21753
TrEMBL
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A0A5E4K8L2_9ARCH
198
0
21766
TrEMBL
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A0A7R9MXU8_9EURY
204
0
21671
TrEMBL
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A0A8D6PTV3_9EURY
213
0
23797
TrEMBL
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A0A401HNZ5_9EURY
217
0
24424
TrEMBL
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A0A8D6PY17_9EURY
213
0
23768
TrEMBL
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A0A5E4HUT1_9ARCH
199
0
21608
TrEMBL
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A0A5E4J8C0_9ARCH
263
0
28663
TrEMBL
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A0A822J000_9EURY
217
0
23713
TrEMBL
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A0A5E4L5Q0_9ARCH
199
0
21848
TrEMBL
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24000
x * 24000, SDS-PAGE
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x * 24000, SDS-PAGE
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x * 24000, SDS-PAGE
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expression in Escherichia coli
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Wang, Y; Xu, H; Jones, MK; White, R.H.
Identification of the final two genes functioning in methanofuran biosynthesis in Methanocaldococcus jannaschii
J. Bacteriol.
197
2850-2858
2015
Methanocaldococcus jannaschii (Q57900), Methanocaldococcus jannaschii DSM 2661 (Q57900)
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