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Information on EC 2.7.3.9 - phosphoenolpyruvate-protein phosphotransferase and Organism(s) Bacillus subtilis and UniProt Accession P08838

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IUBMB Comments
Enzyme I of the phosphotransferase system (cf. EC 2.7.1.69 protein-Npi-phosphohistidine---sugar phosphotransferase). Acts only on histidine residues in specific phosphocarrier proteins of low molecular mass (9.5 kDa) involved in bacterial sugar transport. A similar reaction, where the protein is the enzyme EC 2.7.9.2 pyruvate, water dikinase, is part of the mechanism of that enzyme.
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Bacillus subtilis
UNIPROT: P08838
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The taxonomic range for the selected organisms is: Bacillus subtilis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
enzyme i, phosphoenolpyruvate:sugar phosphotransferase system, phosphoenolpyruvate-dependent phosphotransferase system, phosphoenolpyruvate:carbohydrate phosphotransferase system, phosphoenolpyruvate:glycose phosphotransferase system, phosphoenolpyruvate-dependent phosphotransferase, phosphoenolpyruvate:sugar phosphotransferase, enzyme i of the phosphotransferase system, phosphoenolpyruvate-protein phosphotransferase, phosphotransferase enzyme i, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
enzyme I of the phosphotransferase system
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PEP:sugar phosphotransferase system enzyme I
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phosphoenolpyruvate-protein phosphotransferase
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phosphoenolpyruvate:protein-L-histidine N-pros-phosphotransferase
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phosphotransferase system enzyme I
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enzyme I of the phosphotransferase system
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phosphoenolpyruvate sugar phosphotransferase enzyme I
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phosphoenolpyruvate-dependent phosphotransferase system
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phosphopyruvate-protein factor phosphotransferase
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phosphopyruvate-protein phosphotransferase
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phosphotransferase system enzyme I
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phosphotransferase, phosphoenolpyruvate-protein
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sugar-PEP phosphotransferase enzyme I
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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phospho group transfer
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-
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SYSTEMATIC NAME
IUBMB Comments
phosphoenolpyruvate:protein-L-histidine Npi-phosphotransferase
Enzyme I of the phosphotransferase system (cf. EC 2.7.1.69 protein-Npi-phosphohistidine---sugar phosphotransferase). Acts only on histidine residues in specific phosphocarrier proteins of low molecular mass (9.5 kDa) involved in bacterial sugar transport. A similar reaction, where the protein is the enzyme EC 2.7.9.2 pyruvate, water dikinase, is part of the mechanism of that enzyme.
CAS REGISTRY NUMBER
COMMENTARY hide
37278-17-4
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
phosphoenolpyruvate + protein histidine
pyruvate + protein N-pros-phosphohistidine
show the reaction diagram
HPr, heat stable histidine containing protein, HPr(Ser-P) heat stable histidine containing protein phosphorylated at serine 46, HPr(His-P) heat stable histidine containing protein phosphorylated at histidine 15, HPr-P2 heat stable histidine containing protein doubly phosphorylated, HPr(S46D) heat stable histidine containing protein in which serine is replaced by asparate
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-
?
phosphoenolpyruvate + SacX
pyruvate + phosphorylated SacX
show the reaction diagram
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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50 mM used in assay conditions
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.1
HPr(Ser-P) as substrate
7.9
HPr as substrate
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.9 - 7.5
HPr(Ser-P) as substrate
6.5 - 8.5
HPr as substrate
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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enzyme expression enables cells to grow with maltose, GlcNAc, sucrose, and trehalose
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
130000
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
of the recombinant protein
Talon metal affinity resin column chromatography or Strep-Tactin resin column chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expressed in Escherichia coli strain JW2409-1
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Casabon, I.; Couture, M.; Vaillancourt, K.; Vadeboncoeur, C.
Synthesis of HPr(Ser-P)(His-P) by enzyme I of the phosphoenolpyruvate: sugar phosphotransferase system of Streptococcus salivarius
Biochemistry
45
6692-6702
2006
Bacillus subtilis (P08838), Bacillus subtilis, Streptococcus salivarius (P30299), Streptococcus salivarius
Manually annotated by BRENDA team
Morabbi Heravi, K.; Altenbuchner, J.
Cross talk among transporters of the phosphoenolpyruvate-dependent phosphotransferase system in Bacillus subtilis
J. Bacteriol.
200
e00213-18
2018
Bacillus subtilis, Bacillus subtilis KM0
Manually annotated by BRENDA team