Information on EC 2.7.3.8 - ammonia kinase

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The expected taxonomic range for this enzyme is: Saccharomyces cerevisiae

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EC NUMBER
COMMENTARY hide
2.7.3.8
-
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RECOMMENDED NAME
GeneOntology No.
ammonia kinase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + NH3 = ADP + phosphoramide
show the reaction diagram
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
ATP:ammonia phosphotransferase
Has a wide specificity. In the reverse direction, N-phosphoglycine and N-phosphohistidine can also act as phosphate donors, and ADP, dADP, GDP, CDP, dTDP, dCDP, IDP and UDP can act as phosphate acceptors (in decreasing order of activity).
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CAS REGISTRY NUMBER
COMMENTARY hide
37278-16-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + phosphoiodohistidine
ATP + iodohistidine
show the reaction diagram
-
L-isomer and DL-racemate of phosphoiodohistidine
-
-
?
ADP + phosphoramide
ATP + NH3
show the reaction diagram
-
-
-
-
?
CDP + phosphoramide
CTP + NH3
show the reaction diagram
-
-
-
-
?
dADP + phosphoramide
dATP + NH3
show the reaction diagram
-
-
-
-
?
dCDP + phosphoramide
dCTP + NH3
show the reaction diagram
-
-
-
-
?
GDP + phosphoramide
GTP + NH3
show the reaction diagram
-
-
-
-
?
IDP + phosphoramide
ITP + NH3
show the reaction diagram
-
-
-
-
?
TDP + phosphoramide
TTP + NH3
show the reaction diagram
-
-
-
-
?
UDP + phosphoramide
UTP + NH3
show the reaction diagram
-
-
-
-
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Fe2+
-
1.67 mM, 48% inhibition
Hg2+
-
0.083 mM, 76% inhibition
iodoacetate
-
1.67 mM, 12% inhibition
Mg2+
-
133 mM, complete inhibition, inhibits reaction with phosphormaide or phosphoiodohistidine
Mn2+
-
1.67 mM, 49% inhibition
NEM
-
1.67 mM, 44% inhibition
oligomycin
-
0.267 mM, 42% inhibition
p-chloromercuribenzoate
-
0.83 mM, 95% inhibition
PO43-
-
1.67 mM, 26% inhibition
Sodium arsenate
-
1.67 mM, 65% inhibition
Sodium azide
-
0.267 mM, 42% inhibition
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.115
ADP
-
pH 7.2
0.139
phosphoramide
-
pH 7.2
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.9
-
reaction with phosphoiodohistidine, double pH maximum with peaks at 6.0 and 7.8
7 - 7.2
-
reaction with phosphoramide
7.8
-
reaction with phosphoiodohistidine, double pH maximum with peaks at 6.0 and 7.8
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
pH 6.0: about 60% of maximal activity, pH 8.0: about 70% of maximal activity, reaction with phosphoramide
6.5 - 8.5
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pH 6.5: about 50% of maximal activity, pH 8.5: about 40% of maximal activity, reaction with phosphoiodohistidine
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
-
10 min, denaturation above
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 2.7.3.8)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)