the mutation produces a dramatic change in guanidine substrate specificity, resulting in a shift of substrate specificity from lombricine to taurocyamine, the K95Y mutant functions not as lombricine kinase but as taurocyamine kinase
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0°C, 0.033 M phosphate buffer, pH 7, or 0.05 M Tris-HCl buffer, pH 7.5, or 0.01 M glycylglycine buffer, pH 7, several weeks stable, or in 75% saturated ammonium sulfate solution, pH 8 stable for several months
Der Terrossian, E.; Pradel, L.A.; Kassab, R.; Desvages, G.
Separation of the two non-identical subunits of lombricine kinase from Lumbricus terrestris muscle by chromatography on sepharose-mercurial. Isolation of the tryptic peptide containing its essential thiol group