We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
The taxonomic range for the selected organisms is: Penaeus vannamei The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
arginine kinase, argk, arginine phosphokinase, tcak2, pyak3, tcak1, mnak2, tbak3, pyak2, pyak4,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
adenosine 5'-triphosphate-arginine phosphotransferase
-
-
-
-
adenosine 5'-triphosphate: L-arginine phosphotransferase
-
-
-
-
arginine phosphokinase
-
-
-
-
ATP: arginine N-phosphotransferase
-
ATP:L-arginine N-phosphotransferase
-
-
-
-
kinase, arginine (phosphorylating)
-
-
-
-
ArgK
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
phospho group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP:L-arginine Nomega-phosphotransferase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP + L-Arg
ADP + omega-N-phospho-L-Arg
arginine kinase is an allergenic protein
-
-
?
ATP + L-arginine
ADP + Nomega-phospho-L-arginine
-
-
-
r
ATP + L-Arg
ADP + Nomega-phospho-L-Arg
ATP + L-Arg
ADP + Nomega-phospho-L-Arg
-
-
-
?
ATP + L-Arg
ADP + Nomega-phospho-L-Arg
-
-
-
-
r
ATP + L-Arg
ADP + Nomega-phospho-L-Arg
-
-
-
r
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP + L-arginine
ADP + Nomega-phospho-L-arginine
-
-
-
r
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Mg2+
required
Mg2+
-
required for activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-oxoglutarate
the enzyme activity is inhibited by 10 mM 2-oxoglutarate
ATP
the enzyme is inhibited by 200 mM ATP
D-glucose
the enzyme is inhibited by 50 mM D-glucose, almost all arginine kinase activity is lost after treatment with 200 mM D-glucose
additional information
arginine kinase activity does not show significant variation after incubated with 10-200 mM L-citrulline, L-ornaline, and glycerol
-
additional information
-
arginine kinase activity does not show significant variation after incubated with 10-200 mM L-citrulline, L-ornaline, and glycerol
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP
after treatment with 10 mM ATP, the enzyme activity significantly increases
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
SwissProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
very low mRNA expression
brenda
very low mRNA expression
brenda
-
brenda
-
brenda
very low mRNA expression
brenda
very low mRNA expression
brenda
-
brenda
subcuticular skin, lowest mRNA expression in the skin
brenda
-
brenda
-
brenda
abdominal muscle
brenda
-
-
brenda
highest mRNA expression in the muscle
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
physiological function
arginine kinase is a key enzyme for energetic balance in invertebrates and plays an important role in invertebrate physiology by buffering the ATP pool accordingly to cellular energy requirements
physiological function
arginine kinase may play an important role in the coupling of energy production and utilization and the immune response in shrimps
additional information
the arginine guanidinium group makes ionic contacts with Glu225, Cys271 and a network of ordered water molecules. On the zwitterionic side of the amino acid, the backbone amide nitrogens of Gly64 and Val65 coordinate the arginine carboxylate. Glu314, one of proposed acid-base catalytic residues, does not interact with arginine in the binary complex. Residue Glu324 is located in the flexible loop 310-320 that covers the active site and only stabilizes in the ternary transition state analogue complex, LvAK-TSAC
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KARG0_PENVA
356
0
40160
Swiss-Prot
other Location (Reliability: 1 )
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
40000
x * 40000, about, SDS-PAGE
40000
SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
x * 40000, about, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
purified arginine kinase in binary complex with arginine and in a ternary transition state analogue complex, mixing of 20 mg/ml protein in 10 mM Tris-HCl, pH 8.0, and 1 mM DTT, with with 4 mM L-arginine, 0.001 ml of the solution is then mixed with 0.001 ml of crystallization solution containing 0.2 M sodium acetate, 0.1 M sodium cacodylate, pH 6.5, and 30% w/v PEG 8000, 16°C, 3 weeks. For the ternary complex, the enzyme is cocrystallized with ADP 16 mM, MgCl2 20 mM, L-arginine 40 mM, and NaNO3 75 mM, X-ray diffraction structure determination and analysis at 1.6-1.9 A resolution
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
native enzyme from abdominal muscles
glutathione Sepharose 4B column chromatography
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expressed in Escherichia coli BL21 (DE3) codon plus cells by prokaryotic expression plasmid pGEX-4T-2 as glutathione S-transferase arginine kinase fusion protein
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Garcia-Orozco, K.D.; Aispuro-Hernandez, E.; Yepiz-Plascencia, G.; Calderon-de-la-Barca, A.M.; Sotelo-Mundo, R.R.
Molecular characterization of arginine kinase, an allergen from the shrimp Litopenaeus vannamei
Int. Arch. Allergy Immunol.
144
23-28
2007
Penaeus vannamei, Penaeus vannamei (Q004B5)
brenda
Yao, C.L.; Ji, P.F.; Kong, P.; Wang, Z.Y.; Xiang, J.H.
Arginine kinase from Litopenaeus vannamei: cloning, expression and catalytic properties
Fish Shellfish Immunol.
26
553-558
2009
Penaeus vannamei (B0FRF9), Penaeus vannamei
brenda
Ortea, I.; Canas, B.; Gallardo, J.M.
Mass spectrometry characterization of species-specific peptides from arginine kinase for the identification of commercially relevant shrimp species
J. Proteome Res.
8
5356-5362
2009
Pandalus borealis, Penaeus monodon, Penaeus vannamei, Penaeus notialis, Penaeus indicus, Penaeus merguiensis, Pleoticus muelleri
brenda
Lopez-Zavala, A.A.; Garcia-Orozco, K.D.; Carrasco-Miranda, J.S.; Sugich-Miranda, R.; Velazquez-Contreras, E.F.; Criscitiello, M.F.; Brieba, L.G.; Rudino-Pinera, E.; Sotelo-Mundo, R.R.
Crystal structure of shrimp arginine kinase in binary complex with arginine-a molecular view of the phosphagen precursor binding to the enzyme
J. Bioenerg. Biomembr.
45
511-518
2013
Penaeus vannamei (Q004B5)
brenda