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The taxonomic range for the selected organisms is: Metapenaeus ensis
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
arginine kinase, argk, arginine phosphokinase, pyak3, tcak1, mnak2, tcak2, tbak3, pyak2, pyak4,
more
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ATP: L-arginine phosphotransferase
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adenosine 5'-triphosphate-arginine phosphotransferase
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adenosine 5'-triphosphate: L-arginine phosphotransferase
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arginine phosphokinase
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ATP:L-arginine N-phosphotransferase
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kinase, arginine (phosphorylating)
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phospho group transfer
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ATP:L-arginine Nomega-phosphotransferase
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ATP + L-Arg
ADP + Nomega-phospho-L-Arg
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?
ATP + L-Arg
ADP + Nomega-phospho-L-Arg
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2.33
ATP
purified recombinant enzyme
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8.5
in the process of forward reaction
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30
in the process of forward reaction
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Greasyback shrimp
UniProt
brenda
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brenda
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KARG_METEN
357
0
40263
Swiss-Prot
other Location (Reliability: 1)
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D62E
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the mutant retains almost 90% of the wild type activity
D62G
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the mutant has a pronounced loss in activity
R193G
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the mutant has a pronounced loss in activity
R193K
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the mutant retains almost 90% of the wild type activity
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CM-cellulose column chromatography, Sephacryl S-100 gel filtration, and DEAE-Sepharose column chromatography
nickel affinity column chromatography
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cloned in prokaryotic expression plasmid pET-28a, and then expressed in Escherichia coil strain Rosetta in dissoluble form
expressed in Escherichia coli Rosetta cells
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Wang, J.S.; Zheng, Z.L.; Lei, J.; Pan, J.C.; Zou, G.L.
Cloning, expression, characterization and phylogenetic analysis of arginine kinase from greasyback shrimp (Metapenaeus ensis)
Comp. Biochem. Physiol. B
153
268-274
2009
Metapenaeus ensis (B1PVZ9), Metapenaeus ensis
brenda
Liu, N.; Wang, J.S.; Wang, W.D.; Pan, J.C.
The interaction between residues 62 and 193 play a key role in activity and structural stability of arginine kinase
Int. J. Biol. Macromol.
49
402-408
2011
Metapenaeus ensis
brenda