The taxonomic range for the selected organisms is: Saccharolobus solfataricus The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
the enzyme shows hyperbolic saturation kinetics for 3-phospho-D-glycerate, ATP and 3-phospho-D-glyceroyl phosphate. For ADP it shows a high affinity binding for the reaction in the reverse direction and a low affinity inhibitory effect for the forward as well as the reverse reaction
the enzyme shows hyperbolic saturation kinetics for 3-phospho-D-glycerate, ATP and 3-phospho-D-glyceroyl phosphate. For ADP it shows a high affinity binding for the reaction in the reverse direction and a low affinity inhibitory effect for the forward as well as the reverse reaction
expression in Escherichia coli. The phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase genes from Sulfolobus solfataricus overlap by 8-bp
the gene encoding phosphoglycerate kinase is inserted into a yeast expression vector under the control of the galactose-inducible GAL1 yeast promoter. This vector is then transformed into a pgk::TRP1 yeast mutant, a strain inhibited for growth on galactose or glucose due to its lack of PGK enzyme. Slow-growing transformants are obtained on galactose plates at 37°C, but not at 28°C. These transformants contain low levels of transcripts of the heterologous gene and low amounts of thermostable PGK activity. Weak expression of the hyperthermophile gene in yeast, a mesophile, therefore enables complementation of the yeast pgk defect at 37°C but not at 28°C
Complementation of a pgk deletion mutation in Saccharomyces cerevisiae with expression of the phosphoglycerate-kinase gene from the hyperthermophilic archaeon Sulfolobus solfataricus
The phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase genes from the thermophilic archaeon Sulfolobus solfataricus overlap by 8-bp. Isolation, sequencing of the genes and expression in Escherichia coli
Intermediate instability at high temperature leads to low pathway efficiency for an in vitro reconstituted system of gluconeogenesis in Sulfolobus solfataricus