show all | hide all No of entries

Information on EC 2.7.13.3 - histidine kinase and Organism(s) Staphylococcus aureus

for references in articles please use BRENDA:EC2.7.13.3
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.13 Protein-histidine kinases
                2.7.13.3 histidine kinase
IUBMB Comments
This entry has been included to accommodate those protein-histidine kinases for which the phosphorylation site has not been established (i.e. either the pros- or tele-nitrogen of histidine). A number of histones can act as acceptor.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Staphylococcus aureus
Word Map
The taxonomic range for the selected organisms is: Staphylococcus aureus
The enzyme appears in selected viruses and cellular organisms
Synonyms
33277 FimS, AaHSK1, adaptive-response sensory-kinase A, aerobic respiration control sensor protein arcB, aerobic respiration control sensor protein arcB homolog, aerobic respiration control sensor/response regulatory protein, AfGcHK, AgrC, AHK, AHK5, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
receptor histidine kinase
-
-
sensor histidine kinase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:protein-L-histidine N-phosphotransferase
This entry has been included to accommodate those protein-histidine kinases for which the phosphorylation site has not been established (i.e. either the pros- or tele-nitrogen of histidine). A number of histones can act as acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
99283-67-7
protein-histidine kinases, EC 2.7.13.1, EC 2.7.13.2, and EC 2.7.13.3 are not distinguished in Chemical Abstracts
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
in vitro autokinase activity of recombinant truncated enzyme mutant AgrCTM5-6C in N,N-dimethyldodecylamine N-oxide proteoliposomes
-
-
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
required
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(4-[2-[4-(4-cyanobenzyl)-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl]ethoxy]phenyl)methanaminium chloride
-
-
2-(4-[2-[4-(4-chlorobenzyl)-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl]ethoxy]phenyl)ethanaminium chloride
-
-
2-(4-[2-[4-(4-cyanobenzyl)-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl]ethoxy]phenyl)ethanaminium chloride
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
auto-inducible protein I
-
dose-dependent activation by autoinducing peptide
-
DTT
-
required
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
kinase activity of recombinant enzyme AgrCTM5-6C in N,N-dimethyldodecylamine N-oxide micelles or proteoliposomes
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
an integral membrane protein, protein transmembrane topology in proteoliposomes is determined using membrane-impermeable and membrane-permeable thiol-reactive reagents, overview
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
SRRB_STAAU
583
2
66106
Swiss-Prot
WALK_STAAU
415
0
47818
Swiss-Prot
O07911_STAAU
430
6
49897
TrEMBL
Q2VFQ9_STAAU
430
6
49770
TrEMBL
A0A5C8X4X0_STAAU
344
0
39047
TrEMBL
A0A5C8X4S7_STAAU
368
0
42178
TrEMBL
A0A2X2M8T1_STAAU
243
0
28218
TrEMBL
A0A380E1Y4_STAAU
233
0
26519
TrEMBL
A0A0U1MCV2_STAAU
885
4
102011
TrEMBL
A0A0U1MQM5_STAAU
457
2
52729
TrEMBL
A0A455WGG6_STAAU
344
0
39192
TrEMBL
A0A380EEI1_STAAU
395
0
44050
TrEMBL
A0A1L6C0H1_STAAU
608
2
69917
TrEMBL
A0A2X2K105_STAAU
145
0
16540
TrEMBL
A0A2X2K273_STAAU
280
2
32089
TrEMBL
A0A1L6C0I7_STAAU
608
2
69938
TrEMBL
A0A389V7C6_STAAU
889
4
101956
TrEMBL
A0A1L6C0F6_STAAU
608
2
69960
TrEMBL
A0A1L6C0J1_STAAU
608
2
69923
TrEMBL
A0A0D6G3C7_STAAU
346
2
41079
TrEMBL
A0A1L6C0E0_STAAU
608
2
69944
TrEMBL
A0A168A956_STAAU
608
2
69954
TrEMBL
A0A077UH69_STAAU
554
2
63878
TrEMBL
A0A380EI17_STAAU
234
0
26484
TrEMBL
A0A380EP12_STAAU
340
2
39235
TrEMBL
A0A2X2JXC1_STAAU
94
1
11089
TrEMBL
A0A380DUZ6_STAAU
256
0
28836
TrEMBL
A0A1L6C0H3_STAAU
608
2
69929
TrEMBL
A0A380DT33_STAAU
279
6
32773
TrEMBL
A0A0U1N0Y7_STAAU
370
0
41830
TrEMBL
A0A380EEI6_STAAU
253
2
30808
TrEMBL
A0A380EKM0_STAAU
65
0
7450
TrEMBL
A0A380EJP4_STAAU
138
0
15584
TrEMBL
A0A168T1Y0_STAAU
344
0
39033
TrEMBL
A0A077VLC3_STAAU
347
2
40045
TrEMBL
A0A2X2MEF7_STAAU
137
0
15639
TrEMBL
A0A0U1MSB2_STAAU
457
2
52372
TrEMBL
A0A0U1MRG1_STAAU
347
2
40061
TrEMBL
A0A0Z0N8A3_STAAU
457
2
52343
TrEMBL
A0A380DU20_STAAU
554
2
63853
TrEMBL
A0A380DVI0_STAAU
747
4
86348
TrEMBL
A0A380EJH9_STAAU
69
0
7873
TrEMBL
A0A1L6C0J0_STAAU
608
2
70094
TrEMBL
A0A0U1MYX0_STAAU
363
5
41658
TrEMBL
A0A380EHZ6_STAAU
203
1
22841
TrEMBL
A0A380E4U1_STAAU
281
2
32738
TrEMBL
A0A380EJF4_STAAU
125
1
14390
TrEMBL
A0A0C5HV46_STAAU
363
5
41631
TrEMBL
A0A266CLT2_STAAU
295
2
34019
TrEMBL
A0A380DT97_STAAU
468
2
53938
TrEMBL
A0A447ZTJ2_STAAU
554
2
63883
TrEMBL
A0A380DXQ1_STAAU
117
0
13477
TrEMBL
A0A380EKY6_STAAU
157
0
17622
TrEMBL
A0A380E3C4_STAAU
370
0
41909
TrEMBL
A0A0U1MMZ0_STAAU
430
6
49715
TrEMBL
A0A380EL19_STAAU
54
0
5901
TrEMBL
A0A380ENY4_STAAU
197
0
22905
TrEMBL
A0A2S6DP21_STAAU
295
2
34045
TrEMBL
A0A125SXT4_STAAU
877
4
100611
TrEMBL
A0A380ELM0_STAAU
140
0
15343
TrEMBL
A0A5C8X5X4_STAAU
491
1
55428
TrEMBL
A0A380EMR4_STAAU
368
0
42261
TrEMBL
A0A380EJV1_STAAU
124
0
14629
TrEMBL
A0A380EMU4_STAAU
146
3
17347
TrEMBL
A0A113WKH2_STAAU
351
2
39728
TrEMBL
A0A0D6HHL7_STAAU
554
2
63905
TrEMBL
A0A1L6C0E5_STAAU
608
2
69967
TrEMBL
A0A1L6C0K2_STAAU
608
2
70000
TrEMBL
A0A163UZE5_STAAU
363
6
41834
TrEMBL
A0A380EM18_STAAU
106
0
11706
TrEMBL
A0A077UGF1_STAAU
583
2
66061
TrEMBL
A0A2X3PBX6_STAAU
346
2
41045
TrEMBL
A0A0U1MTF1_STAAU
344
0
39061
TrEMBL
A0A2X2K4H4_STAAU
101
2
11806
TrEMBL
A0A1L6C0G9_STAAU
608
2
69932
TrEMBL
A0A0D6H061_STAAU
457
2
52375
TrEMBL
A0A133Q146_STAAU
313
2
36168
TrEMBL
D3X7X9_STAAU
100
0
11285
TrEMBL
W8U3I5_STAAU
344
0
39120
TrEMBL
X5ELZ2_STAAU
370
0
41881
TrEMBL
H9BRP9_STAAU
351
2
39714
TrEMBL
A0A077UGZ8_STAAU
344
0
39233
TrEMBL
A0A380DTJ8_STAAU
457
2
52427
TrEMBL
A0A077UHL3_STAAU
457
2
52437
TrEMBL
A0A380EF05_STAAU
247
0
28027
TrEMBL
A0A2X2JZ68_STAAU
98
0
11494
TrEMBL
A0A380EN18_STAAU
200
0
23251
TrEMBL
A0A0U1MPH8_STAAU
554
2
63890
TrEMBL
A0A167RS01_STAAU
885
4
102105
TrEMBL
A0A380E9S1_STAAU
42
1
4915
TrEMBL
A0A380ELZ9_STAAU
75
1
9039
TrEMBL
A0A484W0N8_STAAU
302
3
34621
TrEMBL
A0A090N2G8_STAAU
451
2
52386
TrEMBL
A0A1K7Y4G1_STAAU
457
2
52386
TrEMBL
A0A380E508_STAAU
119
0
13558
TrEMBL
A0A380DWH0_STAAU
457
2
52287
TrEMBL
A0A077UI76_STAAU
608
2
69953
TrEMBL
A0A0U1MDP7_STAAU
313
2
36263
TrEMBL
A0A077UK89_STAAU
885
4
102110
TrEMBL
A0A2X2K2I7_STAAU
170
0
19721
TrEMBL
A0A1L6C0G1_STAAU
608
2
69965
TrEMBL
A0A0U1MFF7_STAAU
518
2
60995
TrEMBL
A0A2X2M3R0_STAAU
84
0
9645
TrEMBL
A0A2X2K595_STAAU
141
0
16081
TrEMBL
A0A068A3K4_STAAU
583
2
66076
TrEMBL
W8U600_STAAU
451
2
52400
TrEMBL
V5YQR2_STAAU
608
2
69924
TrEMBL
Q309Z2_STAAU
430
6
49776
TrEMBL
A0A3S4NSM0_STAAU
885
4
102127
TrEMBL
A0A380EB30_STAAU
572
1
65747
TrEMBL
A0A0U1MTD1_STAAU
608
2
69967
TrEMBL
A0A380E0V5_STAAU
346
2
41058
TrEMBL
A0A2X2JYK7_STAAU
424
1
47295
TrEMBL
A0A2X2M8I9_STAAU
81
0
9190
TrEMBL
A0A2X2KEZ5_STAAU
74
1
8476
TrEMBL
A0A2X2LZB2_STAAU
70
0
8137
TrEMBL
A0A1L6C0H2_STAAU
608
2
69940
TrEMBL
A0A380DHJ9_STAAU
317
0
36964
TrEMBL
A0A1L6C0Z8_STAAU
346
2
41079
TrEMBL
A0A0Z2ZYA5_STAAU
583
2
66120
TrEMBL
A0A2X2JR16_STAAU
163
0
18332
TrEMBL
A0A5C8X2R3_STAAU
386
0
44493
TrEMBL
A0A380DUU1_STAAU
62
0
7041
TrEMBL
A0A2X2JWI5_STAAU
74
0
8733
TrEMBL
A0A1L6C0C0_STAAU
608
2
69938
TrEMBL
A0A380DPE0_STAAU
116
0
13598
TrEMBL
A0A1L6C0E9_STAAU
608
2
69919
TrEMBL
A0A1L6C0D6_STAAU
608
2
69960
TrEMBL
A0A447ZUA4_STAAU
370
0
41849
TrEMBL
A0A077UKF2_STAAU
430
6
49850
TrEMBL
A0A0D6G263_STAAU
351
2
39741
TrEMBL
A0A1L6C0D8_STAAU
608
2
69905
TrEMBL
D9IFM6_STAAU
347
2
39996
TrEMBL
A0A0U1MCZ8_STAAU
344
0
39426
TrEMBL
A0A2X2MD73_STAAU
664
3
76809
TrEMBL
A0A0U1MZ26_STAAU
346
2
40913
TrEMBL
A0A077UJS1_STAAU
363
5
41634
TrEMBL
A0A380DHI4_STAAU
32
0
3515
TrEMBL
A0A1L6C0F3_STAAU
608
2
69938
TrEMBL
A0A380E2K0_STAAU
156
0
18103
TrEMBL
A0A077UMX2_STAAU
370
0
41812
TrEMBL
A0A380EKS1_STAAU
56
0
6685
TrEMBL
A0A447ZRB2_STAAU
363
6
41861
TrEMBL
A0A1Q8DD01_STAAU
488
2
56731
TrEMBL
A0A077UFG5_STAAU
518
2
61057
TrEMBL
A0A0U1MLF8_STAAU
583
2
66076
TrEMBL
A0A484VLT2_STAAU
396
0
45727
TrEMBL
A0A1Q8DC11_STAAU
332
2
38712
TrEMBL
A0A2X2UPT8_STAAU
885
4
102205
TrEMBL
A0A0U1N180_STAAU
451
2
52441
TrEMBL
A0A5A4VM18_STAAU
889
4
102029
TrEMBL
A0A0U1MNK6_STAAU
583
2
66147
TrEMBL
A0A1L6C0D3_STAAU
608
2
70028
TrEMBL
A0A380EL64_STAAU
81
0
9480
TrEMBL
A0A2X2KEC7_STAAU
219
0
25269
TrEMBL
A0A0U1MHZ7_STAAU
351
2
39759
TrEMBL
A0A380E1P7_STAAU
83
0
9198
TrEMBL
A0A077UKN2_STAAU
346
2
41050
TrEMBL
A0A447ZYP9_STAAU
295
2
34059
TrEMBL
A0A3S5E0E2_STAAU
583
2
66032
TrEMBL
A0A0U1MWG6_STAAU
518
2
61038
TrEMBL
A0A077UMD1_STAAU
313
2
36181
TrEMBL
A0A2X2K769_STAAU
334
1
38748
TrEMBL
A0A380DTJ7_STAAU
106
0
11955
TrEMBL
A0A1L6C0D9_STAAU
608
2
69954
TrEMBL
A0A380E1P8_STAAU
256
0
29362
TrEMBL
A0A380E253_STAAU
97
2
10919
TrEMBL
A0A380DJP6_STAAU
94
1
10970
TrEMBL
A0A380EJM6_STAAU
148
0
16864
TrEMBL
A0A2X2K1M3_STAAU
231
0
26201
TrEMBL
A0A1L6C0F8_STAAU
608
2
69992
TrEMBL
A0A380DT60_STAAU
367
0
42239
TrEMBL
A0A1Q8DEX1_STAAU
348
2
40117
TrEMBL
A0A0Z0BY62_STAAU
295
2
34105
TrEMBL
A0A163QZ64_STAAU
889
4
102017
TrEMBL
A0A0U1MGT7_STAAU
346
2
40934
TrEMBL
A0A077VZ79_STAAU
451
2
52413
TrEMBL
A0A0Z0Z682_STAAU
554
2
63928
TrEMBL
A0A2X2JS19_STAAU
287
2
33416
TrEMBL
A0A2X2LZ10_STAAU
67
1
7850
TrEMBL
A0A1L6C0D0_STAAU
608
2
69951
TrEMBL
A0A380DWZ6_STAAU
285
2
33030
TrEMBL
A0A380EEJ7_STAAU
52
0
5643
TrEMBL
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
oligomer
intact YycG HK can form higher-order oligomers at the cell membrane via combinations of the H- and L-dimeric interacting surfaces
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
-
AgrC forms ligand-independent dimers that undergo trans-autophosphorylation upon interaction with autoinducing peptide
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
L128C
site-directed mutagenesis
N44C/G142C
site-directed mutagenesis, the monomeric band intensities of the double mutant are dramatically decreased
N44C/G143C
site-directed mutagenesis, the monomeric band intensities of the double mutant are dramatically decreased
N44C/R146C
site-directed mutagenesis, introducing cysteine substitution at Arg146 of YycG N44C mutant does not show any high molecular weight bands, but it shows only the dimeric band of YycG N44C
S81C
site-directed mutagenesis
L128C
-
site-directed mutagenesis
N44C/G142C
-
site-directed mutagenesis, the monomeric band intensities of the double mutant are dramatically decreased
N44C/G143C
-
site-directed mutagenesis, the monomeric band intensities of the double mutant are dramatically decreased
N44C/R146C
-
site-directed mutagenesis, introducing cysteine substitution at Arg146 of YycG N44C mutant does not show any high molecular weight bands, but it shows only the dimeric band of YycG N44C
S81C
-
site-directed mutagenesis
G394A/G396A
-
completely inactive
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged truncated enzyme from Escherichia coli strain C43(DE3) membranes by ultracentrifugation and affinity chromatography, followed by gel filtration
-
recombinant wild-type and mutant His-tagged enzymes from Escherichia coli strain BL21(DE3) by ultracentrifugation, nickel affinity chromatography, and dialysis. His-tag removal by tobacco etch virus protease is followed by nickel affinity chromatography and gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene agrC, recombinant overexpression of His-tagged and GFP-tagged truncated enzyme from pET-28a-AgrCTM5-6C or pET-28-AgrCTM5-6C-GFP vector in Escherichia coli strain C43(DE3)
-
gene yycG, recombinant expression of wild-type and mutant His-tagged enzymes in Escherichia coli strain BL21(DE3), the SeMet-substituted YycG is produced in Escherichia coli methionine auxotrophic strain B834(DE3)
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
2.5fold enhanced transcription of SaeS after incubation with Perform and SDS
RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
purified recombinant truncated enzyme proteins are reconstituted into liposomes by a detergent-mediated method, effect of different detergents on protein reconstitution efficiency, overview. The highest incorporation is found with N,N-dimethyldode-cylamine N-oxide resulting in a yield of 85%, liposomes are consisting of dioleoyl-phosphatidyl-choline : 1,2-dipalmitoyl-sn-glycero-3-phosphocholine : egg L-alpha-phosphatidic acid : cholesterol at molar ratios of 4:4:1:1, pH 7.4. Determination of the morphology and size of liposome, overview
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mueller-Premru, M.; Zidar, N.; Spik, V.C.; Krope, A.; Kikelj, D.
Benzoxazine series of histidine kinase inhibitors as potential antimicrobial agents with activity against enterococci
Chemotherapy
55
414-417
2009
Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus
Manually annotated by BRENDA team
Schaefer, D.; Lam, T.T.; Geiger, T.; Mainiero, M.; Engelmann, S.; Hussain, M.; Bosserhoff, A.; Frosch, M.; Bischoff, M.; Wolz, C.; Reidl, J.; Sinha, B.
A point mutation in the sensor histidine kinase SaeS of Staphylococcus aureus strain Newman alters the response to biocide exposure
J. Bacteriol.
191
7306-7314
2009
Staphylococcus aureus (Q840P7), Staphylococcus aureus, Staphylococcus aureus Newman (Q840P7)
Manually annotated by BRENDA team
George Cisar, E.A.; Geisinger, E.; Muir, T.W.; Novick, R.P.
Symmetric signalling within asymmetric dimers of the Staphylococcus aureus receptor histidine kinase AgrC
Mol. Microbiol.
74
44-57
2009
Staphylococcus aureus
Manually annotated by BRENDA team
Kim, T.; Choi, J.; Lee, S.; Yeo, K.J.; Cheong, H.K.; Kim, K.K.
Structural studies on the extracellular domain of sensor histidine kinase YycG from Staphylococcus aureus and its functional implications
J. Mol. Biol.
428
3074-3089
2016
Staphylococcus aureus (Q2G2U4), Staphylococcus aureus, Staphylococcus aureus NCTC 8325 (Q2G2U4), Staphylococcus aureus RN4220
Manually annotated by BRENDA team
Wang, L.; Quan, C.; Liu, B.; Wang, J.; Xiong, W.; Zhao, P.; Fan, S.
Functional reconstitution of Staphylococcus aureus truncated AgrC histidine kinase in a model membrane system
PLoS ONE
8
e80400
2013
Staphylococcus aureus
Manually annotated by BRENDA team
Select items on the left to see more content.