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Information on EC 2.7.12.1 - dual-specificity kinase and Organism(s) Mus musculus and UniProt Accession Q9Z188

for references in articles please use BRENDA:EC2.7.12.1
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EC Tree
IUBMB Comments
This family of enzymes can phosphorylate both Ser/Thr and Tyr residues.
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Select one or more organisms in this record:
This record set is specific for:
Mus musculus
UNIPROT: Q9Z188
Word Map
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
The taxonomic range for the selected organisms is: Mus musculus
Reaction Schemes
Synonyms
dyrk1a, dyrk2, dyrk1b, dyrk3, dual specificity kinase, dual specificity protein kinase, dual-specificity protein kinase, dyrk1a kinase, dual-specificity tyrosine phosphorylation-regulated kinase 1a, serine/threonine/tyrosine kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Clik1
-
-
-
-
Clk/Sty protein kinase
266570
-
dual specificity protein kinase TTK
266582
-
dual specificity tyrosine phosphorylated and regulated kinase 1B
278348
-
dual specificity tyrosine phosphorylation-regulated kinase 4
307522
-
dual-specificity tyrosine phosphorylation-regulated kinase 1A
244
-
dual-specificity tyrosine-phosphorylation regulated kinase 1A
dual-specificity tyrosine-regulated kinase 1A
244
-
DYRK1A
DYRK1B
278348
-
DYRK3
285988
-
DYRK4
307522
-
Esk kinase
266582
-
protein kinase CLK1
266570
-
protein kinase CLK2
266565
-
protein kinase CLK3
266566
-
protein kinase CLK4
266567
-
STY protein
266570
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (Ser/Thr- and Tyr-phosphorylating)
This family of enzymes can phosphorylate both Ser/Thr and Tyr residues.
CAS REGISTRY NUMBER
COMMENTARY hide
134549-83-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + peptide DYRKtide
ADP + phosphorylated peptide DYRKtide
show the reaction diagram
synthetic peptide substrate
-
-
?
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
ATP + amphiphysin I
ADP + phosphorylated amphiphysin I
show the reaction diagram
-
-
-
-
?
ATP + biotin-Ttds-TPGSRSRTPSLPT
ADP + phosphorylated biotin-Ttds-TPGSRSRTPSLPT
show the reaction diagram
i.e. peptide PEP3
-
-
?
ATP + biotin-Ttds-VGLLKLASPELER
ADP + phosphorylated biotin-Ttds-VGLLKLASPELER
show the reaction diagram
i.e. peptide PEP285
-
-
?
ATP + caspase-9
ADP + phosphorylated caspase-9
show the reaction diagram
-
-
-
-
?
ATP + CREB
ADP + phosphorylated CREB
show the reaction diagram
-
-
-
-
?
ATP + cyclin L2
ADP + phosphorylated cyclin L2
show the reaction diagram
-
-
-
-
?
ATP + dynamin I
ADP + phosphorylated dynamin I
show the reaction diagram
-
-
-
-
?
ATP + eIF2Bepsilon
ADP + phosphorylated eIF2Bepsilon
show the reaction diagram
-
-
-
-
?
ATP + extracellular MAP kinase kinase
ADP + phospho-[MAP kinase kinase]
show the reaction diagram
-
-
-
-
?
ATP + extracellular signal-regulated kinase
ADP + phospho-[extracellular signal-regulated kinase]
show the reaction diagram
-
-
-
-
?
ATP + Gli1 protein
ADP + phosphorylated Gli1 protein
show the reaction diagram
-
-
-
-
?
ATP + glycogen synthase
ADP + phosphorylated glycogen synthase
show the reaction diagram
-
-
-
-
?
ATP + myelin basic protein
ADP + phosphorylated myelin basic protein
show the reaction diagram
phosphorylation on serine, threonine, and tyrosine residues
-
-
?
ATP + neurodegeneration-related septin 4
ADP + phosphorylated neurodegeneration-related septin 4
show the reaction diagram
-
SEPT4, also known as Pnutl2/CDCrel-2
-
-
?
ATP + NFAT
ADP + phosphorylated NFAT
show the reaction diagram
-
-
-
-
?
ATP + Notch protein
ADP + phosphorylated Notch protein
show the reaction diagram
-
-
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
ATP + RRARKLTATPTPLGG
ADP + RRARKLTApTPTPLGG
show the reaction diagram
i.e. peptide SAPtide
-
-
?
ATP + RRRFRPASPLRGPPK
ADP + RRRFRPApSPLRGPPK
show the reaction diagram
i.e. peptide DYRKtide, designed on the basis of the phosphorylation consensus sequence for DYRK1A
-
-
?
ATP + Ser/Arg-rich splicing factors
ADP + phosphorylated Ser/Arg-rich splicing factor
show the reaction diagram
-
-
-
?
ATP + splicing factor 2
ADP + phosphorylated splicing factor 2
show the reaction diagram
-
-
-
-
?
ATP + splicing factor 3
ADP + phosphorylated splicing factor 3
show the reaction diagram
-
-
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
ATP + SR protein
ADP + hyperphosphorylated SR protein
show the reaction diagram
i.e. serine-rich and arginine-rich proteins
-
?
ATP + STAT3
ADP + phosphorylated STAT3
show the reaction diagram
-
-
-
-
?
ATP + synaptojanin I
ADP + phosphorylated synaptojanin I
show the reaction diagram
-
-
-
-
?
ATP + transcription factor FKHR
ADP + phosphorylated transcription factor FKHR
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
ATP + amphiphysin I
ADP + phosphorylated amphiphysin I
show the reaction diagram
-
-
-
-
?
ATP + caspase-9
ADP + phosphorylated caspase-9
show the reaction diagram
-
-
-
-
?
ATP + CREB
ADP + phosphorylated CREB
show the reaction diagram
-
-
-
-
?
ATP + cyclin L2
ADP + phosphorylated cyclin L2
show the reaction diagram
-
-
-
-
?
ATP + dynamin I
ADP + phosphorylated dynamin I
show the reaction diagram
-
-
-
-
?
ATP + eIF2Bepsilon
ADP + phosphorylated eIF2Bepsilon
show the reaction diagram
-
-
-
-
?
ATP + extracellular MAP kinase kinase
ADP + phospho-[MAP kinase kinase]
show the reaction diagram
-
-
-
-
?
ATP + extracellular signal-regulated kinase
ADP + phospho-[extracellular signal-regulated kinase]
show the reaction diagram
-
-
-
-
?
ATP + Gli1 protein
ADP + phosphorylated Gli1 protein
show the reaction diagram
-
-
-
-
?
ATP + glycogen synthase
ADP + phosphorylated glycogen synthase
show the reaction diagram
-
-
-
-
?
ATP + neurodegeneration-related septin 4
ADP + phosphorylated neurodegeneration-related septin 4
show the reaction diagram
-
SEPT4, also known as Pnutl2/CDCrel-2
-
-
?
ATP + NFAT
ADP + phosphorylated NFAT
show the reaction diagram
-
-
-
-
?
ATP + Notch protein
ADP + phosphorylated Notch protein
show the reaction diagram
-
-
-
-
?
ATP + splicing factor 2
ADP + phosphorylated splicing factor 2
show the reaction diagram
-
-
-
-
?
ATP + splicing factor 3
ADP + phosphorylated splicing factor 3
show the reaction diagram
-
-
-
-
?
ATP + SR protein
ADP + ?
show the reaction diagram
ATP + STAT3
ADP + phosphorylated STAT3
show the reaction diagram
-
-
-
-
?
ATP + synaptojanin I
ADP + phosphorylated synaptojanin I
show the reaction diagram
-
-
-
-
?
ATP + transcription factor FKHR
ADP + phosphorylated transcription factor FKHR
show the reaction diagram
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
harmine
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
autophosphorylation of a tyrosine residue within the activation loop is necessary for full DYRK4 kinase activity
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
assay at
7.4
assay at, recombinant enzyme
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
assay at
30
assay at, recombinant enzyme
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
at least two splicing variants
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
embryonal carcinoma cell line
Manually annotated by BRENDA team
DYRK3 expression in, ex vivo analysis of erythroid progenitor cells of DYRK3-knockout mice
Manually annotated by BRENDA team
DYRK3 expression in
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
most of the DYRK4 isoforms show a preferential cytoplasmic localization
Manually annotated by BRENDA team
one of several splice variants of DYRK4 contains a nuclear localization signal in its extended N-terminus that mediates its interaction with importin alpha3 and alpha5 and that is capable of targeting a heterologous protein to the nucleus. The nucleocytoplasmic mobility of this variant differs from that of a shorter isoform. The long isoform (mDYRK4642) does not exit the nucleus
Manually annotated by BRENDA team
-
enzyme contains a nuclear targeting signal sequence
Manually annotated by BRENDA team
additional information
-
STY protein contains a putative nuclear localization signal
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
enzyme DYRK4 belongs to the dual specificity tyrosine phosphorylation-regulated kinases, DYRKs, a family of conserved protein kinases that play key roles in the regulation of cell differentiation, proliferation, and survival. DYRKs contain a conserved Tyr-Xaa-Tyr motif in the activation loop. Autophosphorylation of a second tyrosine residue within the activation loop is necessary for full DYRK4 kinase activity and is a defining feature of the DYRK family. Family members DYRK1A, DYRK2, and DYRK4 differ in their target recognition sequence, and preference for an arginine residue at position P -3 is a feature of DYRK1A but not of DYRK2 and DYRK4
malfunction
physiological function
additional information
both human and murine DYRK4 genes express several transcripts as a result of alternative splicing, with the two main protein products differing in their N-terminal domain
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
DYR1B_MOUSE
629
0
69178
Swiss-Prot
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65000
x * 65000, splicing variant 1, SDS-PAGE, x * 70000, splicing variant 2, SDS-PAGE, x * 75000, splicing variant 3 from skeletal muscle, SDS-PAGE
70000
x * 65000, splicing variant 1, SDS-PAGE, x * 70000, splicing variant 2, SDS-PAGE, x * 75000, splicing variant 3 from skeletal muscle, SDS-PAGE
75000
x * 65000, splicing variant 1, SDS-PAGE, x * 70000, splicing variant 2, SDS-PAGE, x * 75000, splicing variant 3 from skeletal muscle, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 65000, splicing variant 1, SDS-PAGE, x * 70000, splicing variant 2, SDS-PAGE, x * 75000, splicing variant 3 from skeletal muscle, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, the enzyme occurs in several splicing variants, analysis overview, expression in murine hypothalamic GT1-7 cell line, expression of splicing variants in COS_7 cells and in Escherichia coli
EGFP-tagged DYRK1A is expressed in HEK-293 cell
-
gene DYRK1A, quantitative expression analysis
-
gene DYRK4, DNA and amino acid sequence determination and analysis, splicing variants analysis and comparison to the human splicing variants, expression analysis, overview. Recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS and of the GFP-tagged DYRK4 N-terminus in HEK-293T cells
genomic clones of DYRK3 isolated from a 129svJ Lambda phage library, transgenic mice generated, DYRK3 gene deletion in E14 ES cells using a floxed PGKneo targeting vector, targeted cells injected into C57BL/6J blastocysts, transgenic pA2gata1-DYRK3 mice prepared using an Myc epitope-tagged mDYRK3 cDNA within a pA2gata1 vector
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
Dyrk1A expression can be enhanced by treatment with beta-amyloid or overexpression of the transcription factor E2F1, Dyrk1A activity can be increased by binding of 14-3-3 protein
-
Dyrk1A expression can be reressed by the AP4- geminin complex
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
role of DYRK3 as an erythropoietic suppressor selectively during stress erythropoiesis, possible candidate for targeting by small molecule inhibitors as potentially important anti-anemia agents
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nayler, O.; Stamm, S.; Ullrich, A.
Characterization and comparison of four serine- and arginine-rich (SR) protein kinases
Biochem. J.
326
693-700
1997
Mus musculus (O35491), Mus musculus (O35492), Mus musculus (O35493)
Manually annotated by BRENDA team
Colwill, K.; Pawson, T.; Andrews, B.; Prasad, J.; Manley, J.L.; Bell, J.C.; Duncan, P.I.
The Clk/Sty protein kinase phosphorylates SR splicing factors and regulates their intranuclear distribution
EMBO J.
15
265-275
1996
Mus musculus (P22518)
Manually annotated by BRENDA team
Duncan, P.I.; Howell, B.W.; Marius, R.M.; Drmanic, S.; Douville, E.M.; Bell, J.C.
Alternative splicing of STY, a nuclear dual specificity kinase
J. Biol. Chem.
270
21524-21531
1995
Mus musculus, Mus musculus (P22518)
Manually annotated by BRENDA team
Hanes, J.; von der Kammer, H.; Klaudiny, J.; Scheit, K.H.
Characterization by cDNA cloning of two new human protein kinases. Evidence by sequence comparison of a new family of mammalian protein kinases
J. Mol. Biol.
244
665-672
1994
Homo sapiens (P49759), Homo sapiens (P49760), Homo sapiens (P49761), Mus musculus (P22518)
Manually annotated by BRENDA team
Howell, B.W.; Afar, D.E.; Lew, J.; Douville, E.M.; Icely, P.L.; Gray, D.A.; Bell, J.C.
STY, a tyrosine-phosphorylating enzyme with sequence homology to serine/threonine kinases
Mol. Cell. Biol.
11
568-572
1991
Mus musculus (P22518)
Manually annotated by BRENDA team
Ben-David, Y.; Letwin, K.; Tannock, L.; Bernstein, A.; Pawson, T.
A mammalian protein kinase with potential for serine/threonine and tyrosine phosphorylation is related to cell cycle regulators
EMBO J.
10
317-325
1991
Mus musculus (P22518)
Manually annotated by BRENDA team
Song, W.J.; Sternberg, L.R.; Kasten-Sportes, C.; et al.
Isolation of human and murine homologues of the Drosophila minibrain gene: human homologue maps to 21q22.2 in the Down syndrome 'critical region'
Genomics
38
331-339
1996
Homo sapiens, Homo sapiens (Q13627), Mus musculus, Mus musculus (Q61214)
Manually annotated by BRENDA team
Song, W.J.; Chung, S.H.; Kurnit, D.M.
The murine Dyrk protein maps to chromosome 16, localizes to the nucleus, and can form multimers
Biochem. Biophys. Res. Commun.
231
640-644
1997
Mus musculus (Q61214)
Manually annotated by BRENDA team
Douville, E.M.; Afar, D.E.; Howell, B.W.; Letwin, K.; Tannock, L.; Ben-David, Y.; Pawson, T.; Bell, J.C.
Multiple cDNAs encoding the esk kinase predict transmembrane and intracellular enzyme isoforms
Mol. Cell. Biol.
12
2681-2689
1992
Mus musculus, Mus musculus (P35761)
Manually annotated by BRENDA team
Leder, S.; Czajkowska, H.; Maenz, B.; De Graaf, K.; Barthel, A.; Joost, H.G.; Becker, W.
Alternative splicing variants of dual specificity tyrosine phosphorylated and regulated kinase 1B exhibit distinct patterns of expression and functional properties
Biochem. J.
372
881-888
2003
Homo sapiens, Mus musculus, Mus musculus (Q9Z188)
Manually annotated by BRENDA team
Bogacheva, O.; Bogachev, O.; Menon, M.; Dev, A.; Houde, E.; Valoret, E.I.; Prosser, H.M.; Creasy, C.L.; Pickering, S.J.; Grau, E.; Rance, K.; Livi, G.P.; Karur, V.; Erickson-Miller, C.L.; Wojchowski, D.M.
DYRK3 dual-specificity kinase attenuates erythropoiesis during anemia
J. Biol. Chem.
283
36665-36675
2008
Mus musculus, Mus musculus (Q922Y0)
Manually annotated by BRENDA team
Arque, G.; Fotaki, V.; Fernandez, D.; Martinez de Lagran, M.; Arbones, M.L.; Dierssen, M.
Impaired spatial learning strategies and novel object recognition in mice haploinsufficient for the dual specificity tyrosine-regulated kinase-1A (Dyrk1A)
PLoS ONE
3
e2575
2008
Mus musculus (Q61214)
Manually annotated by BRENDA team
Park, J.; Song, W.J.; Chung, K.C.
Function and regulation of Dyrk1A: towards understanding Down syndrome
Cell. Mol. Life Sci.
66
3235-3240
2009
Mus musculus
Manually annotated by BRENDA team
Arque, G.; de Lagran, M.M.; Arbones, M.L.; Dierssen, M.
Age-associated motor and visuo-spatial learning phenotype in Dyrk1A heterozygous mutant mice
Neurobiol. Dis.
36
312-319
2009
Mus musculus
Manually annotated by BRENDA team
Sitz, J.H.; Baumgaertel, K.; Haemmerle, B.; Papadopoulos, C.; Hekerman, P.; Tejedor, F.J.; Becker, W.; Lutz, B.
The Down syndrome candidate dual-specificity tyrosine phosphorylation-regulated kinase 1A phosphorylates the neurodegeneration-related septin 4
Neuroscience
157
596-605
2008
Mus musculus, Mus musculus (Q61214)
Manually annotated by BRENDA team
Papadopoulos, C.; Arato, K.; Lilienthal, E.; Zerweck, J.; Schutkowski, M.; Chatain, N.; Mueller-Newen, G.; Becker, W.; de la Luna, S
Splice variants of the dual specificity tyrosine phosphorylation-regulated kinase 4 (DYRK4) differ in their subcellular localization and catalytic activity
J. Biol. Chem.
286
5494-5505
2011
Homo sapiens (Q9NR20), Mus musculus (Q8BI55)
Manually annotated by BRENDA team
Abekhoukh, S.; Planque, C.; Ripoll, C.; Urbaniak, P.; Paul, J.-L.; Delabar, J.-M.; Janel, N.
Dyrk1A, a serine/threonine kinase, is involved in ERK and Akt activation in the brain of hyperhomocysteinemic mice
Mol. Neurobiol.
47
105-116
2013
Mus musculus, Mus musculus (Q61214)
Manually annotated by BRENDA team
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