Information on EC 2.7.11.33 - [pyruvate, water dikinase] kinase

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The expected taxonomic range for this enzyme is: Escherichia coli

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EC NUMBER
COMMENTARY hide
2.7.11.33
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RECOMMENDED NAME
GeneOntology No.
[pyruvate, water dikinase] kinase
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REACTION
REACTION DIAGRAM
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ORGANISM
UNIPROT
LITERATURE
ADP + [pyruvate, water dikinase] = AMP + [pyruvate, water dikinase] phosphate
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
ADP:[pyruvate, water dikinase] phosphotransferase
The enzyme from the bacterium Escherichia coli is bifunctional and catalyses both the phosphorylation and dephosphorylation of EC 2.7.9.2, pyruvate, water dikinase. cf. EC 2.7.4.28, ([pyruvate, water dikinase] phosphate) phosphotransferase [1]. The enzyme is specific for a reaction intermediate form of EC 2.7.9.2, where it phosphorylates an active site histidine [1]. It has no activity toward EC 2.7.9.1 pyruvate, phosphate dikinase (cf. EC 2.7.11.32, [pyruvate, phosphate dikinase] kinase).
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ORGANISM
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LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
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LITERATURE
(Substrate)
COMMENTARY
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LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
phosphoenolpyruvate synthase + ADP
[phosphoenolpyruvate synthase]phosphate + AMP
show the reaction diagram
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His residue phosphorylated (active)
His and Thr residues phosphorylated (inactive)
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?
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
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LITERATURE
25000
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4 * 25000, gel filtration, SDS-PAGE
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SUBUNITS
ORGANISM
UNIPROT
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LITERATURE
homotetramer
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4 * 25000, gel filtration, SDS-PAGE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
purified protein stable for at least a week when stored on ice
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
immobilized metal ion affinity chromatography (Ni2+)
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
His-tagged protein expressed in Escherichia coli NM522
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Show AA Sequence (4363 entries)
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LINKS TO OTHER DATABASES (specific for EC-Number 2.7.11.33)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)