Information on EC 2.7.11.29 - low-density-lipoprotein receptor kinase

for references in articles please use BRENDA:EC2.7.11.29
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The expected taxonomic range for this enzyme is: Bos taurus

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EC NUMBER
COMMENTARY hide
2.7.11.29
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RECOMMENDED NAME
GeneOntology No.
low-density-lipoprotein receptor kinase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + [low-density-lipoprotein receptor]-L-serine = ADP + [low-density-lipoprotein receptor]-O-phospho-L-serine
show the reaction diagram
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
ATP:[low-density-lipoprotein receptor]-L-serine O-phosphotransferase
Phosphorylates the last serine residue (Ser-833) in the cytoplasmic domain of the low-density lipoprotein receptor from bovine adrenal cortex. Casein can also act as a substrate but with lower affinity. GTP can act instead of ATP.
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CAS REGISTRY NUMBER
COMMENTARY hide
107445-00-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + casein
?
show the reaction diagram
ATP + low-density lipoprotein receptor
?
show the reaction diagram
-
-
-
-
?
ATP + low-density lipoprotein-L-serine
ADP + low-density lipoprotein O-phospho-L-serine
show the reaction diagram
ATP + phosvitin
?
show the reaction diagram
-
-
-
-
?
ATP + tubulin
?
show the reaction diagram
-
-
-
-
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + low-density lipoprotein receptor
?
show the reaction diagram
-
-
-
-
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
heparin
-
strong
Low-density lipoprotein receptor
-
above 10 nM, spermine protects
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polylysine
-
strong
Trypsin
-
inactivation of activator subunit
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additional information
-
no inhibition by cAMP, cGMP, Ca2+/calmodulin, Ca2+/phosphatidylserine or N-ethylmaleimide
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no activation by polylysine
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000005
Low-density lipoprotein receptor
-
30°C, pH 7.5
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00118
-
casein as substrate
0.0033
-
-
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
assay at
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
120000
-
alphabeta, 1 * 35000-50000 + 1 * 120000, SDS-PAGE and gel filtration, a catalytic subunit for phosphorylation and an activator subunit, activator subunit not required
155000 - 170000
-
gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
high salt concentrations, e.g. 1 mM NaCl, inactivate
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
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LINKS TO OTHER DATABASES (specific for EC-Number 2.7.11.29)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)