Information on EC 2.7.11.29 - low-density-lipoprotein receptor kinase

for references in articles please use BRENDA:EC2.7.11.29
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The expected taxonomic range for this enzyme is: Bos taurus

EC NUMBER
COMMENTARY hide
2.7.11.29
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RECOMMENDED NAME
GeneOntology No.
low-density-lipoprotein receptor kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + [low-density-lipoprotein receptor]-L-serine = ADP + [low-density-lipoprotein receptor]-O-phospho-L-serine
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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SYSTEMATIC NAME
IUBMB Comments
ATP:[low-density-lipoprotein receptor]-L-serine O-phosphotransferase
Phosphorylates the last serine residue (Ser-833) in the cytoplasmic domain of the low-density lipoprotein receptor from bovine adrenal cortex. Casein can also act as a substrate but with lower affinity. GTP can act instead of ATP.
CAS REGISTRY NUMBER
COMMENTARY hide
107445-00-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + casein
?
show the reaction diagram
ATP + low-density lipoprotein receptor
?
show the reaction diagram
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-
-
-
?
ATP + low-density lipoprotein-L-serine
ADP + low-density lipoprotein O-phospho-L-serine
show the reaction diagram
ATP + phosvitin
?
show the reaction diagram
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-
-
-
?
ATP + tubulin
?
show the reaction diagram
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-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + low-density lipoprotein receptor
?
show the reaction diagram
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-
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
heparin
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strong
Low-density lipoprotein receptor
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above 10 nM, spermine protects
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polylysine
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strong
Trypsin
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inactivation of activator subunit
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additional information
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no inhibition by cAMP, cGMP, Ca2+/calmodulin, Ca2+/phosphatidylserine or N-ethylmaleimide
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no activation by polylysine
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000005
Low-density lipoprotein receptor
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30°C, pH 7.5
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00118
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casein as substrate
0.0033
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
120000
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alphabeta, 1 * 35000-50000 + 1 * 120000, SDS-PAGE and gel filtration, a catalytic subunit for phosphorylation and an activator subunit, activator subunit not required
155000 - 170000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
high salt concentrations, e.g. 1 mM NaCl, inactivate
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
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