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Information on EC 2.7.11.24 - mitogen-activated protein kinase and Organism(s) Saccharomyces cerevisiae and UniProt Accession P41808

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EC Tree
IUBMB Comments
Phosphorylation of specific tyrosine and threonine residues in the activation loop of this enzyme by EC 2.7.12.2, mitogen-activated protein kinase kinase (MAPKK) is necessary for enzyme activation. Once activated, the enzyme phosphorylates target substrates on serine or threonine residues followed by a proline . A distinguishing feature of all MAPKs is the conserved sequence Thr-Xaa-Tyr (TXY). Mitogen-activated protein kinase (MAPK) signal transduction pathways are among the most widespread mechanisms of cellular regulation. Mammalian MAPK pathways can be recruited by a wide variety of stimuli including hormones (e.g. insulin and growth hormone), mitogens (e.g. epidermal growth factor and platelet-derived growth factor), vasoactive peptides (e.g. angiotensin-II and endothelin), inflammatory cytokines of the tumour necrosis factor (TNF) family and environmental stresses such as osmotic shock, ionizing radiation and ischaemic injury.
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Saccharomyces cerevisiae
UNIPROT: P41808
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Word Map
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
+
a [protein]-(L-serine/L-threonine)
=
+
a [protein]-(L-serine/L-threonine) phosphate
Synonyms
mapk, p38, erk1/2, p38 mapk, mitogen-activated protein kinase, map kinase, extracellular signal-regulated kinase, p38 mitogen-activated protein kinase, p38mapk, p38 map kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sporulation-specific mitogen-activated protein kinase SMK1
-
cp38a
-
-
-
-
cp38b
-
-
-
-
CSAID binding protein
-
-
-
-
CSBP
-
-
-
-
Cytokine suppressive anti-inflammatory drug binding protein
-
-
-
-
ERK5
-
-
-
-
MAP kinase
MAP kinase MXI2
-
-
-
-
MAP kinase p38 beta
-
-
-
-
MAP kinase p38 delta
-
-
-
-
MAP kinase p38 gamma
-
-
-
-
MAP kinase p38a
-
-
-
-
MAP kinase p38alpha
-
-
-
-
MAP kinase p38b
-
-
-
-
mitogen-activated protein kinase
mitogen-activated protein kinase FUS3
-
mitogen-activated protein kinase HOG1
-
mitogen-activated protein kinase KSS1
-
Mitogen-activated protein kinase p38 beta
-
-
-
-
Mitogen-activated protein kinase p38 delta
-
-
-
-
Mitogen-activated protein kinase p38 gamma
-
-
-
-
Mitogen-activated protein kinase p38a
-
-
-
-
Mitogen-activated protein kinase p38alpha
-
-
-
-
Mitogen-activated protein kinase p38b
-
-
-
-
mitogen-activated protein kinase SLT2/MPK1
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p38b
-
-
-
-
SAPK2A
-
-
-
-
SLT2 (MPK1) MAP kinase homolog
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stress-activated protein kinase 2a
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (MAPKK-activated)
Phosphorylation of specific tyrosine and threonine residues in the activation loop of this enzyme by EC 2.7.12.2, mitogen-activated protein kinase kinase (MAPKK) is necessary for enzyme activation. Once activated, the enzyme phosphorylates target substrates on serine or threonine residues followed by a proline [6]. A distinguishing feature of all MAPKs is the conserved sequence Thr-Xaa-Tyr (TXY). Mitogen-activated protein kinase (MAPK) signal transduction pathways are among the most widespread mechanisms of cellular regulation. Mammalian MAPK pathways can be recruited by a wide variety of stimuli including hormones (e.g. insulin and growth hormone), mitogens (e.g. epidermal growth factor and platelet-derived growth factor), vasoactive peptides (e.g. angiotensin-II and endothelin), inflammatory cytokines of the tumour necrosis factor (TNF) family and environmental stresses such as osmotic shock, ionizing radiation and ischaemic injury.
CAS REGISTRY NUMBER
COMMENTARY hide
142243-02-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
-
-
-
?
ATP + Axl2
ADP + phospho-Axl2
show the reaction diagram
-
substrate of Hog1
-
-
?
ATP + casein
ADP + phosphocasein
show the reaction diagram
-
substrate of Hog1
-
-
?
ATP + cdc42
ADP + phosphorylated cdc42
show the reaction diagram
-
substrate of Gic2
-
-
?
ATP + DNA polymerase II
ADP + phosphorylated DNA polymerase II
show the reaction diagram
-
substrate of Hog1p
-
-
?
ATP + Gic2
ADP + phosphorylated Gic2
show the reaction diagram
-
substrate of Fus3, and of Hog1
-
-
?
ATP + histone H1
ADP + phospho-histone H1
show the reaction diagram
-
substrate of Hog1
-
-
?
ATP + Hog1D
ADP + phospho-Hog1D
show the reaction diagram
-
substrate of Hog1
-
-
?
ATP + Hot1p
ADP + phosphorylated Hot1p
show the reaction diagram
ATP + Hsl1
ADP + phospho-Hsl1
show the reaction diagram
-
substrate of Hog1
-
-
?
ATP + MBP
ADP + phospho-MBP
show the reaction diagram
-
substrate of Hog1
-
-
?
ATP + Mek1
ADP + phospho-Mek1
show the reaction diagram
-
substrate of Hog1
-
-
?
ATP + protein tyrosine kinase 2
ADP + phosphorylated protein tyrosine kinase 2
show the reaction diagram
-
substrate of Hog1
-
-
?
ATP + RAD9
ADP + phospho-RAD9
show the reaction diagram
-
high activity with Fus3, low activity with Hog1
-
-
?
ATP + RAD9p
ADP + phospho-RAD9p
show the reaction diagram
-
substrate of Hog1
-
-
?
ATP + Rck2
ADP + phosphorylated Rck2
show the reaction diagram
-
-
-
-
?
ATP + Red1
ADP + phospho-Red1
show the reaction diagram
-
preferred substrate of Hog1
-
-
?
ATP + Ste50
ADP + phosphorylated Ste50
show the reaction diagram
ATP + Swe1
ADP + phospho-Swe1
show the reaction diagram
-
substrate of Hog1
-
-
?
ATP + Swi6
ADP + phospho-Swi6
show the reaction diagram
-
substrate of Hog1
-
-
?
ATP + Tub4p
ADP + phospho-Tub4
show the reaction diagram
-
substrate of Hog1
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
-
-
-
?
ATP + DNA polymerase II
ADP + phosphorylated DNA polymerase II
show the reaction diagram
-
substrate of Hog1p
-
-
?
ATP + Hot1p
ADP + phosphorylated Hot1p
show the reaction diagram
-
substrate of Hog1p, phosphorylation of Hot1p is not required for Hot1p-mediated gene expression
-
-
?
ATP + Ste50
ADP + phosphorylated Ste50
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
sorbitol
-
upon exposure to 1000 mM sorbitol, Hog1 is phosphorylated rapidly, with modification peaking at 10 to 20 min and then declining as cells adapt
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
signaling kinetics, overview
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
large scale activity assay with recombinant enzyme in a protein chip consisting of a microwell array with protein covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker, overview
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
during growth under isosmotic conditions, presence of Hog1, the Hog1-as–mutant, and mutants K52R and D144A
Manually annotated by BRENDA team
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
transcription factor protein domains consisting of the LXL motif, the FXFP motif, the LXLXXXF motif, or the ETS motif, are involved in stable interaction of MAPKs with transcription complexes
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D144A
-
kinase-dead mutant, is unable to grow on 1000 mM sorbitol
K52R
-
retains residual activity that permitts slow but readily detectable growth on 1000 mM sorbitol, residual growth is eliminated in the presence of 1-NM-PP1
T100A
-
Hog1-as-mutant, fully functional analog-sensitive allele of HOG1, is unable to grow in the presence of 1-NM-PP1, permits acute inhibition of the enzyme without other detectable perturbations of the cell, in the presence of inhibitor Rck2 modification is completely abrogated
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
into vector pCR2.1, expressed in Escherichia coli DH5alpha
-
phylogenetic tree of kinases derived from the kinase core sequence, overview, overexpression as GST-fusion protein under control of the galactose-inducible GAL1 promotor in Escherichia coli, determination of 5'-end sequences
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
-
development of a protein chip consisting of a silicone elastomer microwell array with recombinant enzyme covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker for large scale activity assay, overview
pharmacology
protein scaffolds, e.g. of MAP kinases, provide a flexible platform for reprogramming cellular responses and could be exploited to engineer cells with novel therapeutic and biotechnological functions
additional information
-
a role for active Hog1 in maintaining signaling specificity under conditions of persistently high external osmolarity
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Johnston, M.; Andrews, S.; Brinkman, R.; Cooper, J.; Ding, H.; Dover, J.; Du, Z.; Favello, A.; Fulton, L.; Gattung, S.; et al.
Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII
Science
265
2077-2082
1994
Saccharomyces cerevisiae (Q00772)
Manually annotated by BRENDA team
Rieger, M.; Bruckner, M.; Schafer, M.; Muller-Auer, S.
Sequence analysis of 203 kilobases from Saccharomyces cerevisiae chromosome VII
Yeast
13
1077-1090
1997
Saccharomyces cerevisiae (P14681)
Manually annotated by BRENDA team
Gartner, A.; Nasmyth, K.; Ammerer, G.
Signal transduction in Saccharomyces cerevisiae requires tyrosine and threonine phosphorylation of FUS3 and KSS1
Genes Dev.
6
1280-1292
1992
Saccharomyces cerevisiae (P14681), Saccharomyces cerevisiae (P16892)
Manually annotated by BRENDA team
Courchesne, W.E.; Kunisawa, R.; Thorner, J.
A putative protein kinase overcomes pheromone-induced arrest of cell cycling in S. cerevisiae
Cell
58
1107-1119
1989
Saccharomyces cerevisiae (P14681)
Manually annotated by BRENDA team
Van Dyck, L.; Purnelle, B.; Skala, J.; Goffeau, A.
An 11.4 kb DNA segment on the left arm of yeast chromosome II carries the carboxypeptidase Y sorting gene PEP1, as well as ACH1, FUS3 and a putative ARS
Yeast
8
769-776
1992
Saccharomyces cerevisiae (P16892)
Manually annotated by BRENDA team
Fujimura, H.A.
The DAC2/FUS3 protein kinase is not essential for transcriptional activation of the mating pheromone response pathway in Saccharomyces cerevisiae
Mol. Gen. Genet.
235
450-452
1992
Saccharomyces cerevisiae (P16892)
Manually annotated by BRENDA team
Elion, E.A.; Grisafi, P.L.; Fink, G.R.
FUS3 encodes a cdc2+/CDC28-related kinase required for the transition from mitosis into conjugation
Cell
60
649-664
1990
Saccharomyces cerevisiae (P16892)
Manually annotated by BRENDA team
Verhasselt, P.; Volckaert, G.
Sequence analysis of a 37.6 kbp cosmid clone from the right arm of Saccharomyces cerevisiae chromosome XII, carrying YAP3, HOG1, SNR6, tRNA-Arg3 and 23 new open reading frames, among which several homologies to proteins involved in cell division control and to mammalian growth factors and other animal proteins are found
Yeast
13
241-250
1997
Saccharomyces cerevisiae (P32485)
Manually annotated by BRENDA team
Brewster, J.L.; de Valoir, T.; Dwyer, N.D.; Winter, E.; Gustin, M.C.
An osmosensing signal transduction pathway in yeast
Science
259
1760-1763
1993
Saccharomyces cerevisiae (P32485), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Krisak, L.; Strich, R.; Winters, R.S.; Hall, J.P.; Mallory, M.J.; Kreitzer, D.; Tuan, R.S.; Winter, E.
SMK1, a developmentally regulated MAP kinase, is required for spore wall assembly in Saccharomyces cerevisiae
Genes Dev.
8
2151-2161
1994
Saccharomyces cerevisiae (P41808), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Mazzoni, C.; Zarov, P.; Rambourg, A.; Mann, C.
The SLT2 (MPK1) MAP kinase homolog is involved in polarized cell growth in Saccharomyces cerevisiae
J. Cell. Biol.
123
1821-1833
1993
Saccharomyces cerevisiae (Q00772), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Torres, L.; Martin, H.; Garcia-Saez, M.I.; Arroyo, J.; Molina, M.; Sanchez, M.; Nombela, C.
A protein kinase gene complements the lytic phenotype of Saccharomyces cerevisiae lyt2 mutants
Mol. Microbiol.
5
2845-2854
1991
Saccharomyces cerevisiae (Q00772)
Manually annotated by BRENDA team
Zhu, H.; Klemic, J.F.; Chang, S.; Bertone, P.; Casamayor, A.; Klemic, K.G.; Smith, D.; Gerstein, M.; Reed, M.A.; Snyder, M.
Analysis of yeast protein kinases using protein chips
Nat. Genet.
26
283-289
2000
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Schwartz, M.A.; Madhani, H.D.
Principles of MAP kinase signaling specificity in Saccharomyces cerevisiae
Annu. Rev. Genet.
38
725-748
2004
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Edmunds, J.W.; Mahadevan, L.C.
MAP kinases as structural adaptors and enzymatic activators in transcription complexes
J. Cell Sci.
117
3715-3723
2004
Saccharomyces cerevisiae, Mammalia
Manually annotated by BRENDA team
Westfall, P.J.; Thorner, J.
Analysis of mitogen-activated protein kinase signaling specificity in response to hyperosmotic stress: use of an analog-sensitive HOG1 allele
Eukaryot. Cell
5
1215-1228
2006
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Hao, N.; Zeng, Y.; Elston, T.C.; Dohlman, H.G.
Control of MAP kinase specificity by feedback phosphorylation of a shared adaptor protein Ste50
J. Biol. Chem.
283
33798-33802
2008
Saccharomyces cerevisiae (P14681), Saccharomyces cerevisiae (P32485), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Bashor, C.J.; Helman, N.C.; Yan, S.; Lim, W.A.
Using engineered scaffold interactions to reshape MAP kinase pathway signaling dynamics
Science
319
1539-1543
2008
Saccharomyces cerevisiae (P16892), Saccharomyces cerevisiae
Manually annotated by BRENDA team