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ATP + [DNA-directed RNA polymerase II]
ADP + phospho-[DNA-directed RNA polymerase II]
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
SRB10 phosphorylates the C-terminal domain of RNA polymerase II at Ser2 and Ser5
-
-
?
ATP + C-terminal domain of RNA polymerase II
ADP + phosphorylated C-terminal domain of RNA polymerase II
the recombinant GST-tagged CTD substrate is hyperphosphorylated by Kin28
-
-
?
ATP + CTD-containing fusion protein
ADP + phosphorylated CTD-containing fusion protein
-
-
-
-
?
ATP + CTD-containing fusion proteins
ADP + ?
-
-
-
-
?
ATP + histone H3
?
-
Ctk1 is essential for H3K36 methylation, also regulates H3K4 methylation
-
-
?
ATP + holo-RNA polymerase II
?
-
C-terminal domain phosphorylation by the serine 5-specific TFIIH complex, its kinase module TFIIK or by the C-terminal domain serine 2-specific kinase CTDK1
-
-
?
ATP + RNA polymerase II
?
ATP + RNA polymerase II C-terminal domain
ADP + phosphoylated RNA polymerase II C-terminal domain
ATP + RNA polymerase II C-terminal domain
ADP + phosphoylated RNA polymerase II C-terminal subunit
ATP + RNA polymerase II C-terminal repeat domain
ADP + phosphoylated RNA polymerase II C-terminal repeat domain
ATP + RNA polymerase II C-terminal subunit
ADP + phosphoylated RNA polymerase II C-terminal subunit
ATP + RNA polymerase II carboxy terminal domain
ADP + phosphorylated RNA polymerase II carboxy terminal domain
ATP + Rps2
?
-
phosphorylates on Ser 238, phosphorylation of Rps2 is needed for translational accuracy
-
-
?
ATP + Rps2
ADP + phosphorylated Rps2
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
ATP + [DNA-directed RNA polymerase II]
ADP + phospho-[DNA-directed RNA polymerase II]
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
additional information
?
-
ATP + [DNA-directed RNA polymerase II]
ADP + phospho-[DNA-directed RNA polymerase II]
only Rpb1, the largest subunit of RNAPII evolved a unique, highly repetitive carboxy-terminal domain, termed CTD. Dynamic phosphorylation patterns of serine residues in the CTD during gene transcription. Phosphorylation of Ser2, Ser5, Thr4, and Tyr1 in the CTD. CTD is composed of multiple tandem heptapeptides with the evolutionary conserved consensus motif Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7
-
-
?
ATP + [DNA-directed RNA polymerase II]
ADP + phospho-[DNA-directed RNA polymerase II]
subunit Srb10 of the Mediator phosphorylates CTD Ser5
-
-
?
ATP + RNA polymerase II
?
-
-
-
-
?
ATP + RNA polymerase II
?
-
TFIIH and mediator phosphorylate at C-terminal domain at Ser 5, Ser5 phosphorylation by the yeast Cdk7 (Kin28) subunit of TFIIH is required for recruitment of capping enzyme to the promoter region, P-TEFb phosphorylates at Ser 2
-
-
?
ATP + RNA polymerase II C-terminal domain
ADP + phosphoylated RNA polymerase II C-terminal domain
-
-
-
?
ATP + RNA polymerase II C-terminal domain
ADP + phosphoylated RNA polymerase II C-terminal domain
the SRI domain of Set2 interacts with the phosphorylated CTD of elongating RNAPII leading to methylation of the chromatin during transcription
-
-
?
ATP + RNA polymerase II C-terminal domain
ADP + phosphoylated RNA polymerase II C-terminal subunit
-
Ctk1 kinase activity regulates H3K4 methylation, the Ctk2 and Ctk3 components of CTDK-1 are necessary for Ctk1 kinase activity, overview
-
-
?
ATP + RNA polymerase II C-terminal domain
ADP + phosphoylated RNA polymerase II C-terminal subunit
-
TFIIH, a 10-subunit complex with many resident enzymatic activities, is essential for transcription by RNA polymerase II involving the TFIIH-associated kinase, Cdk7, which phosphorylates the C-terminal domain, CTD, of Rpb1, the largest subunit of Pol II
-
-
?
ATP + RNA polymerase II C-terminal domain
ADP + phosphoylated RNA polymerase II C-terminal subunit
-
Cdk7 phosphorylates the C-terminal domain, CTD, that consists of multiple YSPTSPS heptapeptide repeats, at Ser5
-
-
?
ATP + RNA polymerase II C-terminal domain
ADP + phosphoylated RNA polymerase II C-terminal subunit
-
phosphorylation at Ser5, phosphorylation patterns, overview
-
-
?
ATP + RNA polymerase II C-terminal repeat domain
ADP + phosphoylated RNA polymerase II C-terminal repeat domain
-
hyperphosphorylation, the Mediator complex associates with RNA polymerase II, RNAPII, at least partly via the RNAPII C-terminal repeat domain, CTD, whose phosphorylation is involved in triggering promoter clearance
-
-
?
ATP + RNA polymerase II C-terminal repeat domain
ADP + phosphoylated RNA polymerase II C-terminal repeat domain
-
i.e. RNAPII CTD, hyperphosphorylation, the target specificity of TFIIK and CTDK1 differm, TFIIK phosphorylates Ser5, while CTDK1 phosphorylates Ser2
-
-
?
ATP + RNA polymerase II C-terminal subunit
ADP + phosphoylated RNA polymerase II C-terminal subunit
-
the enzyme regulates transcription elongation at many genes and integrates mRNA synthesis with histone modification, pre-mRNA processing, and mRNA export. Recruitment of P-TEFb to target genes requires deubiquitination of H2Bub, phosphorylation of H3S10, and the bromodomain protein, Brd4, overview. P-TEFb accompanies the mature mRNA to the cytoplasm to promote translation elongation. Enzyme regulation system, detailed overview
-
-
?
ATP + RNA polymerase II C-terminal subunit
ADP + phosphoylated RNA polymerase II C-terminal subunit
-
phosphorylation at Ser2, Ser5, and Ser7, the extended C-terminal domain, CTD, of the Rbp1 subunit contains 52 heptad repeats with a consensus sequence YSPTSPS
-
-
?
ATP + RNA polymerase II carboxy terminal domain
ADP + phosphorylated RNA polymerase II carboxy terminal domain
-
the phosphorylated RNAPII CTD, interacts with BRCA1 for induction of DEF1-dependent cleavage and accumulation of a RNAPII fragment containing the P-CTD, overview
-
-
?
ATP + RNA polymerase II carboxy terminal domain
ADP + phosphorylated RNA polymerase II carboxy terminal domain
-
i.e. RNAPII CTD
-
-
?
ATP + Rps2
ADP + phosphorylated Rps2
-
a protein of the small ribosomal subunit, Ctk1 interacts with the transcription and mRNA export, TREX, complex, which couples transcription to mRNA export, and Ctk1 enhances efficient and accurate translation of the mRNA, Ctk1 is a prerequisite for correct decoding in vivo overview
-
-
?
ATP + Rps2
ADP + phosphorylated Rps2
-
a protein of the small ribosomal subunit, phosphorylation at Ser238
-
-
?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
-
-
-
?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
no substrate is phosvitin
-
-
?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
phosphorylates not Tyr-residues
-
-
?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
phosphorylates to a lesser extent Thr-residues
-
-
?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
phosphorylates predominantly Ser-residues
-
-
?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
presumably obligate part of transcription process
-
-
?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
CTD kinase 1 plays an important role in transcription elongation in vivo, the deletion of one ore more CTK genes is lethal but in combination with the deletion of PPR2 or ELP
-
-
?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
deletion of the kinase subunit Ctk1 results in phosphorylation of serine in position 5 of the CTD repeat during logarithmic growth and eliminates the transient increase in CTD serine 2 phosphorylation during the diauxic shift
-
-
?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
the CTD is essential for viability, although mutants with deletions that remove approximately half of the repeats are still viable
-
-
?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
CTD kinase I affects pre-mRNA 3' cleavage/polyadenylation through the processing component Pti1p
-
-
?
ATP + [DNA-directed RNA polymerase II]
ADP + phospho-[DNA-directed RNA polymerase II]
only Rpb1, the largest subunit of RNAPII evolved a unique, highly repetitive carboxy-terminal domain, termed CTD. Dynamic phosphorylation patterns of serine residues in the CTD during gene transcription. Phosphorylation of Ser2, Ser5, Thr4, and Tyr1 in the CTD. CTD is composed of multiple tandem heptapeptides with the evolutionary conserved consensus motif Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7
-
-
?
ATP + [DNA-directed RNA polymerase II]
ADP + phospho-[DNA-directed RNA polymerase II]
only Rpb1, the largest subunit of RNAPII evolved a unique, highly repetitive carboxy-terminal domain, termed CTD. Dynamic phosphorylation patterns of serine residues in the CTD during gene transcription. Phosphorylation of Ser2, Ser5, Thr4, and Tyr1 in the CTD. CTD is composed of multiple tandem heptapeptides with the evolutionary conserved consensus motif Tyr1Ser2Pro3Thr4Ser5Pro6Ser7
-
-
?
ATP + [DNA-directed RNA polymerase II]
ADP + phospho-[DNA-directed RNA polymerase II]
kinases Bur1 and Ctk1 phosphorylate Ser2
-
-
?
ATP + [DNA-directed RNA polymerase II]
ADP + phospho-[DNA-directed RNA polymerase II]
the cyclin-dependent kinase subunit Kin28 of TFIIH phosphorylates Ser5 and Ser7
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
-
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
CTD phosphorylation facilitates pre-mRNA processing, CTD phosphorylation and transcription cycle overview
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
hyperphosphorylation of the C-terminal domain CTD of the RNA polymerase II large subunit by Ctk1 is essentially required for methylation of histone H3 Lys36 in transcription elongation in volving association of Set2 to the hyperphosphorylated RNA polymerase II, overview
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
hyperphosphorylation of the C-terminal repeat domain CTD of the RNA polymerase II large subunit is required for elongation of mRNA, the enzyme is involved in functional organization of transcription and nuclear metabolism
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
phosphorylation of Ser2 within the RNA polymerase II C-terminal domain couples transcription and 3' end processing by recruiting factors for polyadenylation and 3' end processing, phosphorylation of Ser5 during initiation recruits the capping enzyme
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
the CTD kinases Ctk1, Bur1, Kin28, and Srb10 are involved in preinitiation of transcription and elongation
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
the enzyme is involved, together with several factors, in regulation of RNA elongation, transition at the 3' end, and polyadenylation, the enzyme is responsible for crosslinking of polyadenylation factors, overview
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
the enzyme preferentially phosphorylates RNA poylmerase II bound in a native ternary complex in opposite to the Fcp1 phosphatase preferably dephosphorylating free RNA poylmerase II, after complex disruption, at Ser5, not Ser2, of the CTD, the TFIIH TD kinase is involved in RNA poylmerase II activity regulation, mechanism, overview
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
CTD kinase 1 hyperphosphorylates the C-terminal repeat domain CTD of the RNA polymerase II large subunit, phosphorylation of CTD leads to interaction/binding of several proteins with nuclear functions in vitro, i.e. phosphoCTD-associating proteins, purification and analysis of PCAPs, e.g. Ess1, Hrr25, Prp40, Ssd1, SSd1, and Set2, overview
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
Ctk1 kinase phosphorylates Ser2 and Ser5 of the C-terminal CTD domain of the RNA polymerase II large subunit
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
Ctk1 kinase phosphorylates the C-terminal CTD domain of the RNA polymerase II large subunit, primarily at Ser2 and Ser5
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
hyperphosphorylation of the C-terminal domain CTD of the RNA polymerase II large subunit
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
phosphorylation of the C-terminal domain CTD of the RNA polymerase II large subunit at Ser2 and Ser5
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
recombinant GST-tagged protein substrate or synthetic GST-tagged peptide substrate derived from RNA polymerase II, CTD kinase 1 hyperphosphorylates the C-terminal repeat domain CTD of the RNA polymerase II large subunit at Ser2 and/or Ser5, determination of phosphorylation sites, already phosphorylated substrates are more efficient substrates for CTD kinase I
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
the enzyme phosphorylates the C-terminal CTD domain of the RNA polymerase II large subunit
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
the enzyme phosphorylates the C-terminal CTD domain of the RNA polymerase II large subunit, the CTD phosphorylation pattern is precisely modified as RNA polymerase II progresses along the genes and is involved in sequential recruitment of RNA processing factors, multiple phosphorylation sites and epitopes, e.g. at Ser5, overview
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
Ctk1 is the major serine 2 kinase in vivo
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
CTK1 phosphorylates the C-terminal domain of RNA polymerase II at Ser2 and Ser5
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
KIN28 phosphorylates the C-terminal domain of RNA polymerase II at Ser2 and Ser5
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
in vitro, Ctk1 complex can phosphorylate the CTD at all three serines, although with different efficiencies. Ctk1 phosphorylates Ser2 in vitro
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
phosphorylation of Ser2, Ser5, and Ser7 in CTD
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
phosphorylation of Ser5 and Ser7 in CTD, Bur1 phosphorylates Ser2 in vitro
-
-
?
additional information
?
-
the CTD differs in length dependent on the complexity of the organism. While Saccharomyces cerevisiae has 26 repeats, which nearly all obey the consensus sequence, mammalian CTD comprises 52. Dynamic changes in the CTD phosphorylation pattern due to a complex interplay of various kinases and phosphatases subsequently orchestrate the binding of CTD interacting proteins, cf. CTD code
-
-
?
additional information
?
-
the CTD differs in length dependent on the complexity of the organism. While Saccharomyces cerevisiae has 26 repeats, which nearly all obey the consensus sequence, mammalian CTD comprises 52. Dynamic changes in the CTD phosphorylation pattern due to a complex interplay of various kinases and phosphatases subsequently orchestrate the binding of CTD interacting proteins, cf. CTD code
-
-
?
additional information
?
-
the CTD differs in length dependent on the complexity of the organism. While Saccharomyces cerevisiae has 26 repeats, which nearly all obey the consensus sequence, mammalian CTD comprises 52. Dynamic changes in the CTD phosphorylation pattern due to a complex interplay of various kinases and phosphatases subsequently orchestrate the binding of CTD interacting proteins, cf. CTD code
-
-
?
additional information
?
-
the CTD differs in length dependent on the complexity of the organism. While Saccharomyces cerevisiae has 26 repeats, which nearly all obey the consensus sequence, mammalian CTD comprises 52. Dynamic changes in the CTD phosphorylation pattern due to a complex interplay of various kinases and phosphatases subsequently orchestrate the binding of CTD interacting proteins, cf. CTD code
-
-
?
additional information
?
-
-
CTD kinase function is opposed by Ess1, an essential prolyl isomerase binding to the C-terminal domain of the RNA polymerase, Ess1 interacts with CTD kinases, especially with Ctk1 and Srb10, the kinase and Ess1 compete for Ser5 of RNA polymerase II
-
-
?
additional information
?
-
-
the reaction is also in vitro performed by cyclin-dependent kinase Ctk1, and Bur1, EC 2.7.11.22, phosphorylating specific serine residues
-
-
?
additional information
?
-
-
CTK1 kinase is required for BRCA1-induced lethality in yeast, overview
-
-
?
additional information
?
-
-
P-TEFb co-operates with c-Myc during transactivation and cell transformation, and also requires SKIP, c-Ski-interacting protein, an mRNA elongationand splicing factor. Some functions of the P-TEFb/Ser2P CTD are executed by the Spt6 transcription elongation factor, which binds directly to the phosphorylated CTD and recruits the Iws1 protein, overview. Because P-TEFb counteracts NELF- and DSIF-induced pausing in cell-free transcription reactions, at least part of its activity does not depend upon chromatin
-
-
?
additional information
?
-
Set2-catalyzed H3K36me2 does not require either Ctk1-dependent phosphorylation of RNA polymerase II or the presence of the phospho-C-terminal domain, CTD, interaction (SRI) domain of Set2 for methylation of histone H3 at lysine 36 promoting deacetylation of transcribed chromatin and repressing cryptic promoters within genes. By contrast, H3K36me3 requires Spt6, proline 38 on histone H3, the CTD of RNAPII, Ctk1, and the C-terminal SRI domain of Set2, overview
-
-
?
additional information
?
-
-
enzyme-Mediator complex dissociation assay, overview
-
-
?
additional information
?
-
the CTD differs in length dependent on the complexity of the organism. While Saccharomyces cerevisiae has 26 repeats, which nearly all obey the consensus sequence, mammalian CTD comprises 52. Dynamic changes in the CTD phosphorylation pattern due to a complex interplay of various kinases and phosphatases subsequently orchestrate the binding of CTD interacting proteins, cf. CTD code
-
-
?
additional information
?
-
the CTD differs in length dependent on the complexity of the organism. While Saccharomyces cerevisiae has 26 repeats, which nearly all obey the consensus sequence, mammalian CTD comprises 52. Dynamic changes in the CTD phosphorylation pattern due to a complex interplay of various kinases and phosphatases subsequently orchestrate the binding of CTD interacting proteins, cf. CTD code
-
-
?
additional information
?
-
the CTD differs in length dependent on the complexity of the organism. While Saccharomyces cerevisiae has 26 repeats, which nearly all obey the consensus sequence, mammalian CTD comprises 52. Dynamic changes in the CTD phosphorylation pattern due to a complex interplay of various kinases and phosphatases subsequently orchestrate the binding of CTD interacting proteins, cf. CTD code
-
-
?
additional information
?
-
the CTD differs in length dependent on the complexity of the organism. While Saccharomyces cerevisiae has 26 repeats, which nearly all obey the consensus sequence, mammalian CTD comprises 52. Dynamic changes in the CTD phosphorylation pattern due to a complex interplay of various kinases and phosphatases subsequently orchestrate the binding of CTD interacting proteins, cf. CTD code
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ATP + [DNA-directed RNA polymerase II]
ADP + phospho-[DNA-directed RNA polymerase II]
only Rpb1, the largest subunit of RNAPII evolved a unique, highly repetitive carboxy-terminal domain, termed CTD. Dynamic phosphorylation patterns of serine residues in the CTD during gene transcription. Phosphorylation of Ser2, Ser5, Thr4, and Tyr1 in the CTD. CTD is composed of multiple tandem heptapeptides with the evolutionary conserved consensus motif Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
SRB10 phosphorylates the C-terminal domain of RNA polymerase II at Ser2 and Ser5
-
-
?
ATP + RNA polymerase II C-terminal domain
ADP + phosphoylated RNA polymerase II C-terminal domain
the SRI domain of Set2 interacts with the phosphorylated CTD of elongating RNAPII leading to methylation of the chromatin during transcription
-
-
?
ATP + RNA polymerase II C-terminal domain
ADP + phosphoylated RNA polymerase II C-terminal subunit
ATP + RNA polymerase II C-terminal repeat domain
ADP + phosphoylated RNA polymerase II C-terminal repeat domain
-
hyperphosphorylation, the Mediator complex associates with RNA polymerase II, RNAPII, at least partly via the RNAPII C-terminal repeat domain, CTD, whose phosphorylation is involved in triggering promoter clearance
-
-
?
ATP + RNA polymerase II C-terminal subunit
ADP + phosphoylated RNA polymerase II C-terminal subunit
-
the enzyme regulates transcription elongation at many genes and integrates mRNA synthesis with histone modification, pre-mRNA processing, and mRNA export. Recruitment of P-TEFb to target genes requires deubiquitination of H2Bub, phosphorylation of H3S10, and the bromodomain protein, Brd4, overview. P-TEFb accompanies the mature mRNA to the cytoplasm to promote translation elongation. Enzyme regulation system, detailed overview
-
-
?
ATP + RNA polymerase II carboxy terminal domain
ADP + phosphorylated RNA polymerase II carboxy terminal domain
-
the phosphorylated RNAPII CTD, interacts with BRCA1 for induction of DEF1-dependent cleavage and accumulation of a RNAPII fragment containing the P-CTD, overview
-
-
?
ATP + Rps2
ADP + phosphorylated Rps2
-
a protein of the small ribosomal subunit, Ctk1 interacts with the transcription and mRNA export, TREX, complex, which couples transcription to mRNA export, and Ctk1 enhances efficient and accurate translation of the mRNA, Ctk1 is a prerequisite for correct decoding in vivo overview
-
-
?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
ATP + [DNA-directed RNA polymerase II]
ADP + phospho-[DNA-directed RNA polymerase II]
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
additional information
?
-
ATP + RNA polymerase II C-terminal domain
ADP + phosphoylated RNA polymerase II C-terminal subunit
-
Ctk1 kinase activity regulates H3K4 methylation, the Ctk2 and Ctk3 components of CTDK-1 are necessary for Ctk1 kinase activity, overview
-
-
?
ATP + RNA polymerase II C-terminal domain
ADP + phosphoylated RNA polymerase II C-terminal subunit
-
TFIIH, a 10-subunit complex with many resident enzymatic activities, is essential for transcription by RNA polymerase II involving the TFIIH-associated kinase, Cdk7, which phosphorylates the C-terminal domain, CTD, of Rpb1, the largest subunit of Pol II
-
-
?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
-
-
-
?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
presumably obligate part of transcription process
-
-
?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
CTD kinase 1 plays an important role in transcription elongation in vivo, the deletion of one ore more CTK genes is lethal but in combination with the deletion of PPR2 or ELP
-
-
?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
deletion of the kinase subunit Ctk1 results in phosphorylation of serine in position 5 of the CTD repeat during logarithmic growth and eliminates the transient increase in CTD serine 2 phosphorylation during the diauxic shift
-
-
?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
the CTD is essential for viability, although mutants with deletions that remove approximately half of the repeats are still viable
-
-
?
ATP + [DNA-directed eukaryotic RNA polymerase II subunit IIa]
ADP + phospho-[DNA-directed RNA polymerase II subunit IIa]
-
CTD kinase I affects pre-mRNA 3' cleavage/polyadenylation through the processing component Pti1p
-
-
?
ATP + [DNA-directed RNA polymerase II]
ADP + phospho-[DNA-directed RNA polymerase II]
only Rpb1, the largest subunit of RNAPII evolved a unique, highly repetitive carboxy-terminal domain, termed CTD. Dynamic phosphorylation patterns of serine residues in the CTD during gene transcription. Phosphorylation of Ser2, Ser5, Thr4, and Tyr1 in the CTD. CTD is composed of multiple tandem heptapeptides with the evolutionary conserved consensus motif Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7
-
-
?
ATP + [DNA-directed RNA polymerase II]
ADP + phospho-[DNA-directed RNA polymerase II]
only Rpb1, the largest subunit of RNAPII evolved a unique, highly repetitive carboxy-terminal domain, termed CTD. Dynamic phosphorylation patterns of serine residues in the CTD during gene transcription. Phosphorylation of Ser2, Ser5, Thr4, and Tyr1 in the CTD. CTD is composed of multiple tandem heptapeptides with the evolutionary conserved consensus motif Tyr1Ser2Pro3Thr4Ser5Pro6Ser7
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
-
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
CTD phosphorylation facilitates pre-mRNA processing, CTD phosphorylation and transcription cycle overview
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
hyperphosphorylation of the C-terminal domain CTD of the RNA polymerase II large subunit by Ctk1 is essentially required for methylation of histone H3 Lys36 in transcription elongation in volving association of Set2 to the hyperphosphorylated RNA polymerase II, overview
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
hyperphosphorylation of the C-terminal repeat domain CTD of the RNA polymerase II large subunit is required for elongation of mRNA, the enzyme is involved in functional organization of transcription and nuclear metabolism
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
phosphorylation of Ser2 within the RNA polymerase II C-terminal domain couples transcription and 3' end processing by recruiting factors for polyadenylation and 3' end processing, phosphorylation of Ser5 during initiation recruits the capping enzyme
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
the CTD kinases Ctk1, Bur1, Kin28, and Srb10 are involved in preinitiation of transcription and elongation
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
the enzyme is involved, together with several factors, in regulation of RNA elongation, transition at the 3' end, and polyadenylation, the enzyme is responsible for crosslinking of polyadenylation factors, overview
-
-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
-
the enzyme preferentially phosphorylates RNA poylmerase II bound in a native ternary complex in opposite to the Fcp1 phosphatase preferably dephosphorylating free RNA poylmerase II, after complex disruption, at Ser5, not Ser2, of the CTD, the TFIIH TD kinase is involved in RNA poylmerase II activity regulation, mechanism, overview
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-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
Ctk1 is the major serine 2 kinase in vivo
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-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
CTK1 phosphorylates the C-terminal domain of RNA polymerase II at Ser2 and Ser5
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-
?
ATP + [DNA-directed RNA polymerase]
ADP + phospho-[DNA-directed RNA polymerase]
KIN28 phosphorylates the C-terminal domain of RNA polymerase II at Ser2 and Ser5
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-
?
additional information
?
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the CTD differs in length dependent on the complexity of the organism. While Saccharomyces cerevisiae has 26 repeats, which nearly all obey the consensus sequence, mammalian CTD comprises 52. Dynamic changes in the CTD phosphorylation pattern due to a complex interplay of various kinases and phosphatases subsequently orchestrate the binding of CTD interacting proteins, cf. CTD code
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?
additional information
?
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the CTD differs in length dependent on the complexity of the organism. While Saccharomyces cerevisiae has 26 repeats, which nearly all obey the consensus sequence, mammalian CTD comprises 52. Dynamic changes in the CTD phosphorylation pattern due to a complex interplay of various kinases and phosphatases subsequently orchestrate the binding of CTD interacting proteins, cf. CTD code
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-
?
additional information
?
-
the CTD differs in length dependent on the complexity of the organism. While Saccharomyces cerevisiae has 26 repeats, which nearly all obey the consensus sequence, mammalian CTD comprises 52. Dynamic changes in the CTD phosphorylation pattern due to a complex interplay of various kinases and phosphatases subsequently orchestrate the binding of CTD interacting proteins, cf. CTD code
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-
?
additional information
?
-
the CTD differs in length dependent on the complexity of the organism. While Saccharomyces cerevisiae has 26 repeats, which nearly all obey the consensus sequence, mammalian CTD comprises 52. Dynamic changes in the CTD phosphorylation pattern due to a complex interplay of various kinases and phosphatases subsequently orchestrate the binding of CTD interacting proteins, cf. CTD code
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-
?
additional information
?
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-
CTD kinase function is opposed by Ess1, an essential prolyl isomerase binding to the C-terminal domain of the RNA polymerase, Ess1 interacts with CTD kinases, especially with Ctk1 and Srb10, the kinase and Ess1 compete for Ser5 of RNA polymerase II
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additional information
?
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CTK1 kinase is required for BRCA1-induced lethality in yeast, overview
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?
additional information
?
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P-TEFb co-operates with c-Myc during transactivation and cell transformation, and also requires SKIP, c-Ski-interacting protein, an mRNA elongationand splicing factor. Some functions of the P-TEFb/Ser2P CTD are executed by the Spt6 transcription elongation factor, which binds directly to the phosphorylated CTD and recruits the Iws1 protein, overview. Because P-TEFb counteracts NELF- and DSIF-induced pausing in cell-free transcription reactions, at least part of its activity does not depend upon chromatin
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?
additional information
?
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Set2-catalyzed H3K36me2 does not require either Ctk1-dependent phosphorylation of RNA polymerase II or the presence of the phospho-C-terminal domain, CTD, interaction (SRI) domain of Set2 for methylation of histone H3 at lysine 36 promoting deacetylation of transcribed chromatin and repressing cryptic promoters within genes. By contrast, H3K36me3 requires Spt6, proline 38 on histone H3, the CTD of RNAPII, Ctk1, and the C-terminal SRI domain of Set2, overview
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?
additional information
?
-
the CTD differs in length dependent on the complexity of the organism. While Saccharomyces cerevisiae has 26 repeats, which nearly all obey the consensus sequence, mammalian CTD comprises 52. Dynamic changes in the CTD phosphorylation pattern due to a complex interplay of various kinases and phosphatases subsequently orchestrate the binding of CTD interacting proteins, cf. CTD code
-
-
?
additional information
?
-
the CTD differs in length dependent on the complexity of the organism. While Saccharomyces cerevisiae has 26 repeats, which nearly all obey the consensus sequence, mammalian CTD comprises 52. Dynamic changes in the CTD phosphorylation pattern due to a complex interplay of various kinases and phosphatases subsequently orchestrate the binding of CTD interacting proteins, cf. CTD code
-
-
?
additional information
?
-
the CTD differs in length dependent on the complexity of the organism. While Saccharomyces cerevisiae has 26 repeats, which nearly all obey the consensus sequence, mammalian CTD comprises 52. Dynamic changes in the CTD phosphorylation pattern due to a complex interplay of various kinases and phosphatases subsequently orchestrate the binding of CTD interacting proteins, cf. CTD code
-
-
?
additional information
?
-
the CTD differs in length dependent on the complexity of the organism. While Saccharomyces cerevisiae has 26 repeats, which nearly all obey the consensus sequence, mammalian CTD comprises 52. Dynamic changes in the CTD phosphorylation pattern due to a complex interplay of various kinases and phosphatases subsequently orchestrate the binding of CTD interacting proteins, cf. CTD code
-
-
?
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279
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