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Information on EC 2.7.11.22 - cyclin-dependent kinase and Organism(s) Xenopus laevis and UniProt Accession P23437
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2.7.11.22 cyclin-dependent kinaseIUBMB Comments
Activation of cyclin-dependent kinases requires association of the enzyme with a regulatory subunit referred to as a cyclin. It is the sequential activation and inactivation of cyclin-dependent kinases, through the periodic synthesis and destruction of cyclins, that provides the primary means of cell-cycle regulation.
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Word Map
- 2.7.11.22
- cyclins
- p27kip1
- retinoblastoma
-
mitosis
-
cell-cycle
-
checkpoint
- histone
- tumorigenesis
- tumour
- bcl-2
- p16ink4a
-
s-phase
-
hyperphosphorylation
- leukemia
- prostate
- chromatin
- epidermal
-
exit
-
antiproliferative
- mapks
- caspase-3
-
mitogen
- tau
- xenopus
- spindle
- c-myc
-
interphase
-
quiescent
- oocyte
- pcna
-
fission
- alzheimer
- proteasome
- microtubule
- analysis
- medicine
- cdc25c
- pharmacology
- letrozole
-
cycle-dependent
-
polo-like
- chk1
-
p53-independent
- drug development
-
cytokinesis
- centrosome
-
prophase
-
postmitotic
-
receptor-positive
-
cycle-related
-
anaphase
-
aurora
-
metaphase
-
her2-negative
The taxonomic range for the selected organisms is: Xenopus laevis
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
+
a [protein]-(L-serine/L-threonine)
=
+
a [protein]-(L-serine/L-threonine) phosphate
Synonyms
cyclin-dependent kinase, cdk, p34cdc2, cdkl5, cdc2 kinase, cyclin-dependent kinase 5, cyclin-dependent kinase 2, cdc28, cyclin-dependent kinase 4, cyclin-dependent kinase 1, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
cell division protein kinase 2 homolog
-
Cyclin-dependent kinase pef1
-
-
-
-
PHO85 homolog
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:cyclin phosphotransferase
Activation of cyclin-dependent kinases requires association of the enzyme with a regulatory subunit referred to as a cyclin. It is the sequential activation and inactivation of cyclin-dependent kinases, through the periodic synthesis and destruction of cyclins, that provides the primary means of cell-cycle regulation.
CAS REGISTRY NUMBER
COMMENTARY
150428-23-2
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + acidic alpha-syntrophin protein
ADP + acidic alpha-syntrophin phosphoprotein
-
substrate of cyclinA/CDK2, not of cyclin E/CDK2
-
-
?
ATP + ADP-ribosylation factor GTPase activating protein 3 protein
ADP + ADP-ribosylation factor GTPase activating protein 3 phosphoprotein
-
substrate of cyclin B/CDK1 and cyclinA/CDK2
-
-
?
ATP + AE binding protein 2 protein
ADP + AE binding protein 2 phosphoprotein
-
substrate of cyclinA/CDK2 and cyclin E/CDK2
-
-
?
ATP + APEX nuclease protein
ADP + APEX nuclease phosphoprotein
-
substrate of cyclinA/CDK2 and cyclin E/CDK2
-
-
?
ATP + ARAF serine/threonine protein kinase protein
ADP + ARAF serine/threonine protein kinase phosphoprotein
-
substrate of cyclinA/CDK2 and cyclin E/CDK2
-
-
?
ATP + ATRIP protein
ADP + ATRIP phosphoprotein
-
substrate of cyclinA/CDK2 and cyclin E/CDK2
-
-
?
ATP + cAMP responsive element binding protein 3-like 2 protein
ADP + cAMP responsive element binding protein 3-like 2 phosphoprotein
-
substrate of cyclinA/CDK2, not of cyclin E/CDK2
-
-
?
ATP + CCAAT/enhancer binding protein gamma protein
ADP + CCAAT/enhancer binding protein gamma phosphoprotein
-
substrate of cyclin B/CDK1 and cyclinA/CDK2
-
-
?
ATP + cdc2
ADP + phosphorylated cdc2
phosphorylation of Thr161
-
-
?
ATP + cdc2-like protein
ADP + phosphorylated cdc2-like protein from Caenorhabditis in chimeric complexes including both mitotic and G1/S cyclins
from Caenorhabditis in chimeric complexes including both mitotic and G1/S cyclins
-
-
?
ATP + coiled-coil domain protein 52 protein
ADP + coiled-coil domain protein 52 phosphoprotein
-
substrate of cyclin E/CDK2, not of cyclinA/CDK2
-
-
?
ATP + CPEB-associated factor Maskin protein
ADP + CPEB-associated factor Maskin phosphoprotein
-
substrate of cyclinA/CDK2 and cyclin E/CDK2
-
-
?
ATP + diacylglycerol kinase epsilon protein
ADP + diacylglycerol kinase epsilon phosphoprotein
-
substrate of cyclin B/CDK1 and cyclinA/CDK2
-
-
?
ATP + diphthamide biosynthesis protein 2 isoform a protein
ADP + diphthamide biosynthesis protein 2 isoform a phosphoprotein
-
substrate of cyclin B/CDK1 and cyclinA/CDK2
-
-
?
ATP + distal-less homeobox 1 isoform 1 protein
ADP + distal-less homeobox 1 isoform 1 phosphoprotein
-
substrate of cyclin B/CDK1 and cyclinA/CDK2
-
-
?
ATP + Erp1/emi2 protein
ADP + Erp1/emi2 phosphoprotein
-
substrate of cyclinA/CDK2 and cyclin E/CDK2
-
-
?
ATP + establishment of cohesion-1 homologue 2 protein
ADP + establishment of cohesion-1 homologue 2 phosphoprotein
-
substrate of cyclinA/CDK2 and cyclin E/CDK2
-
-
?
ATP + ETS variant gene-1 protein
ADP + ETS variant gene-1 phosphoprotein
-
substrate of cyclin B/CDK1 and cyclinA/CDK2
-
-
?
ATP + Fizzy1 protein
ADP + Fizzy1 phosphoprotein
-
substrate of cyclin E/CDK2, not of cyclinA/CDK2
-
-
?
ATP + glycogenin protein
ADP + glycogenin phosphoprotein
-
substrate of cyclin B/CDK1, not of cyclinA/CDK2
-
-
?
ATP + granulin isoform 1 precursor protein
ADP + granulin isoform 1 precursor phosphoprotein
-
substrate of cyclinA/CDK2 and cyclin E/CDK2
-
-
?
ATP + heat shock transcription factor 1 protein
ADP + heat shock transcription factor 1 phosphoprotein
-
substrate of cyclin B/CDK1 and cyclinA/CDK2
-
-
?
ATP + Hermes protein
ADP + Hermes phosphoprotein
-
substrate of cyclin B/CDK1 and cyclinA/CDK2
-
-
?
ATP + histone deacetylase 6 protein
ADP + histone deacetylase 6 phosphoprotein
-
substrate of cyclin B/CDK1 and cyclinA/CDK2
-
-
?
ATP + histone stem-loop binding protein
ADP + histone stem-loop binding phosphoprotein
-
substrate of cyclinA/CDK2 and cyclin E/CDK2
-
-
?
ATP + Kunitz type serine protease inhibitor 2 protein
ADP + Kunitz type serine protease inhibitor 2 phosphoprotein
-
substrate of cyclin B/CDK1 and cyclinA/CDK2
-
-
?
ATP + lamina-associated protein 2-beta isoform protein
ADP + lamina-associated protein 2-beta isoform phosphoprotein
-
substrate of cyclinA/CDK2, not of cyclin E/CDK2
-
-
?
ATP + LOC222229 protein
ADP + LOC222229 phosphoprotein
-
substrate of cyclin B/CDK1 and cyclinA/CDK2
-
-
?
ATP + low density lipoprotein receptor adaptor protein 1 protein
ADP + low density lipoprotein receptor adaptor protein 1 phosphoprotein
-
substrate of cyclin B/CDK1 and cyclinA/CDK2
-
-
?
ATP + MAP/microtubule affinity regulating kinase 3 protein
ADP + MAP/microtubule affinity regulating kinase 3 phosphoprotein
-
substrate of cyclin B/CDK1 and cyclinA/CDK2
-
-
?
ATP + maternal embryonic leucine zipper kinase protein
ADP + maternal embryonic leucine zipper kinase phosphoprotein
-
substrate of cyclinA/CDK2 and cyclin E/CDK2
-
-
?
ATP + MGC86234 protein
ADP + MGC86234 phosphoprotein
-
i.e. potassium channel tetramerisation domaincontaining 3, substrate of cyclinA/CDK2, not of cyclin E/CDK2
-
-
?
ATP + minor histocompatibility antigen HA-1 protein
ADP + minor histocompatibility antigen HA-1 phosphoprotein
-
substrate of cyclinA/CDK2 and cyclin E/CDK2
-
-
?
ATP + neuronal cytoskeletal protein NF-H
ADP + phosphorylated neuronal cytoskeletal protein NF-H
-
-
-
?
ATP + neuronal cytoskeletal protein tau
ADP + phosphorylated neuronal cytoskeletal protein tau
-
-
-
?
ATP + neuronal cytoskeletal proteins NF-M
ADP + phosphorylated neuronal cytoskeletal protein NF-M
-
-
-
?
ATP + palmitoylated membrane protein 1 protein
ADP + palmitoylated membrane protein 1 phosphoprotein
-
substrate of cyclin B/CDK1 and cyclinA/CDK2
-
-
?
ATP + PASCIN protein
ADP + PASCIN phosphoprotein
-
substrate of cyclin B/CDK1 and cyclinA/CDK2
-
-
?
ATP + phosphatidylinositol-4-phosphate 5-kinase type IIba protein
ADP + phosphatidylinositol-4-phosphate 5-kinase type IIba phosphoprotein
-
substrate of cyclinA/CDK2, not of cyclin E/CDK2
-
-
?
ATP + PIF1 protein
ADP + PIF1 phosphoprotein
-
substrate of cyclinA/CDK2 and cyclin E/CDK2
-
-
?
ATP + POM21 protein
ADP + POM21 phosphoprotein
-
substrate of cyclinA/CDK2 and cyclin E/CDK2
-
-
?
ATP + pre-B-cell leukemia transcription factor 1 protein
ADP + pre-B-cell leukemia transcription factor 1 phosphoprotein
-
substrate of cyclinA/CDK2, not of cyclin B/CDK1
-
-
?
ATP + RNA polymerase 1 protein
ADP + RNA polymerase 1 phosphoprotein
-
substrate of cyclin B/CDK1 and cyclinA/CDK2
-
-
?
ATP + SEC14-like 1 isoform a protein
ADP + SEC14-like 1 isoform a phosphoprotein
-
substrate of cyclin B/CDK1 and cyclinA/CDK2
-
-
?
ATP + semaphorin 6D protein
ADP + semaphorin 6D phosphoprotein
-
substrate of cyclinA/CDK2 and cyclin E/CDK2
-
-
?
ATP + similar to bicaudal C homologue 1 protein
ADP + similar to bicaudal C homologue 1 phosphoprotein
-
substrate of cyclinA/CDK2 and cyclin E/CDK2
-
-
?
ATP + similar to peroxisomal biogenesis factor 5 protein
ADP + similar to peroxisomal biogenesis factor 5 phosphoprotein
-
substrate of cyclin B/CDK1 and cyclinA/CDK2
-
-
?
ATP + similar to Trigger of mitotic entry 1 protein
ADP + similar to Trigger of mitotic entry 1 phosphoprotein
-
substrate of cyclin B/CDK1 and cyclinA/CDK2
-
-
?
ATP + similar to Zinc finger protein 516 protein
ADP + similar to Zinc finger protein 516 phosphoprotein
-
substrate of cyclinA/CDK2 and cyclin E/CDK2
-
-
?
ATP + SMARCD2/BAF60:SWI/SNF-related matrix-associated actin-dependent regulator of chromatin d2 protein
ADP + SMARCD2/BAF60:SWI/SNF-related matrix-associated actin-dependent regulator of chromatin d2 phosphoprotein
-
substrate of cyclin B/CDK1 and cyclinA/CDK2
-
-
?
ATP + SmcX-Jumonji/ARID domain-containing protein 1C protein
ADP + SmcX-Jumonji/ARID domain-containing protein 1C phosphoprotein
-
substrate of cyclinA/CDK2 and cyclin E/CDK2
-
-
?
ATP + striatin protein
ADP + striatin phosphoprotein
-
substrate of cyclin E/CDK2, not of cyclinA/CDK2
-
-
?
ATP + TATA box-binding protein-associated factor 2F protein
ADP + TATA box-binding protein-associated factor 2F phosphoprotein
-
substrate of cyclin B/CDK1, not of cyclinA/CDK2
-
-
?
ATP + telomeric repeat binding factor 2 protein
ADP + telomeric repeat binding factor 2 phosphoprotein
-
substrate of cyclin B/CDK1 and cyclinA/CDK2
-
-
?
ATP + Tipin protein
ADP + Tipin phosphoprotein
-
substrate of cyclinA/CDK2 and cyclin E/CDK2
-
-
?
ATP + v-raf murine sarcoma viral oncogene homolog B1 protein
ADP + v-raf murine sarcoma viraloncogene homolog B1 phosphoprotein
-
substrate of cyclin B/CDK1 and cyclinA/CDK2
-
-
?
ATP + Xenopus laevis protein associated with PRK1 protein
ADP + Xenopus laevis protein associated with PRK1 phosphoprotein
-
substrate of cyclinA/CDK2, not of cyclin E/CDK2
-
-
?
ATP + zinc finger protein 46 protein
ADP + zinc finger protein 46 phosphoprotein
-
substrate of cyclinA/CDK2, not of cyclin E/CDK2
-
-
?
ATP + Cdc20
ADP + phosphorylated Cdc20
-
substrate of Cdk1, rather than of Cdk2
-
-
?
ATP + Cdc20
ADP + phosphorylated Cdc20
-
in vitro substrate of Cdk1 and Cdk2
-
-
?
additional information
?
-
enzyme is required both for entry into S phase and mitosis
-
-
?
additional information
?
-
enzyme is involved in negative regulation of meiotic maturation of Xenopus oocytes
-
-
?
additional information
?
-
-
Cdks are cell cycle regulating enzymes, Cdk1 phosphorylates the anaphase-promoting complex-cyclosome during mitosis, which is a prerequisite for its activity but reduces the anaphase-promoting complex-cyclosome interaction with Cdc20 involving Mad2, the spindle checkpoint requires cyclin-dependent kinase activity, inhibition of Cdk overrides checkpoint-dependent arrest in eggs increasing the interaction of the anaphase-promoting complex-cyclosome with Cdc20, regulation overview
-
-
?
additional information
?
-
-
identification 35 substrates from screening for cyclin B/CDK1, and investigation of the differential specificity of cyclin B/CDK1 versus cyclin A/CDK1 and cyclin A/CDK2. About half the substrates are equally well phosphorylated regardless of the cyclin or the CDK, computational analysis, overview
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + Cdc20
ADP + phosphorylated Cdc20
-
substrate of Cdk1, rather than of Cdk2
-
-
?
additional information
?
-
enzyme is required both for entry into S phase and mitosis
-
-
?
additional information
?
-
enzyme is involved in negative regulation of meiotic maturation of Xenopus oocytes
-
-
?
additional information
?
-
-
Cdks are cell cycle regulating enzymes, Cdk1 phosphorylates the anaphase-promoting complex-cyclosome during mitosis, which is a prerequisite for its activity but reduces the anaphase-promoting complex-cyclosome interaction with Cdc20 involving Mad2, the spindle checkpoint requires cyclin-dependent kinase activity, inhibition of Cdk overrides checkpoint-dependent arrest in eggs increasing the interaction of the anaphase-promoting complex-cyclosome with Cdc20, regulation overview
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). CDK2_XENLA
297
0
33870
Swiss-Prot
other Location (Reliability: 1)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
T14A
mutant enzyme does not induce germinal vesicle breakdown upon microinjection into oocytes
T14A/Y15F
mutant enzyme does induce germinal vesicle breakdown upon microinjection into oocytes
T14A/Y15F/T161A
mutant enzyme fails to induce germinal vesicle breakdown in oocytes and shows a decreased binding to cyclin B1 in coimmunoprecipitations
T14A/Y15F/T161E
mutant enzyme fails to induce germinal vesicle breakdown in oocytes and shows a decreased binding to cyclin B1 in coimmunoprecipitations
T161A
mutant enzyme fails to induce germinal vesicle breakdown in oocytes and shows a decreased binding to cyclin B1 in coimmunoprecipitations
T161E
mutant enzyme fails to induce germinal vesicle breakdown in oocytes and shows a decreased binding to cyclin B1 in coimmunoprecipitations
Y15F
mutant enzyme does not induce germinal vesicle breakdown upon microinjection into oocytes
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Labbe, J.C.; Martinez, A.M.; Fesquet, D.; Capony, J.P.; Darbon, J.M.; Derancourt, J.; Devault, A.; Morin, N.; Cavadore, J.C.; Doree, M.
p40MO15 associates with a p36 subunit and requires both nuclear translocation and Thr176 phosphorylation to generate cdk-activating kinase activity in Xenopus oocytes
EMBO J.
13
5155-5164
1994
Xenopus laevis (P20911)
Shuttleworth, J.; Godfrey, R.; Colman, A.
p40MO15, a cdc2-related protein kinase involved in negative regulation of meiotic maturation of Xenopus oocytes
EMBO J.
9
3233-3240
1990
Xenopus laevis (P20911)
Poon, R.Y.; Yamashita, K.; Adamczewski, J.P.; Hunt, T.; Shuttleworth, J.
The cdc2-related protein p40MO15 is the catalytic subunit of a protein kinase that can activate p33cdk2 and p34cdc2
EMBO J.
12
3123-3132
1993
Xenopus laevis (P23437)
Paris, J.; Le Guellec, R.; Couturier, A.; Le Guellec, K.; Omilli, F.; Camonis, J.; MacNeill, S.; Philippe, M.
Cloning by differential screening of a Xenopus cDNA coding for a protein highly homologous to cdc2
Proc. Natl. Acad. Sci. USA
88
1039-1043
1991
Xenopus laevis (P23437), Xenopus laevis
Stern, B.; Ried, G.; Clegg, N.J.; Grigliatti, T.A.; Lehner, C.F.
Genetic analysis of the Drosophila cdc2 homolog
Development
117
219-232
1993
Xenopus laevis (P23437)
Clegg, N.J.; Whitehead, I.P.; Williams, J.A.; Spiegelman, G.B.; Grigliatti, T.A.
A developmental and molecular analysis of Cdc2 mutations in Drosophila melanogaster
Genome
36
676-685
1993
Xenopus laevis (P23437)
Jimenez, J.; Alphey, L.; Nurse, P.; Glover, D.M.
Complementation of fission yeast cdc2ts and cdc25ts mutants identifies two cell cycle genes from Drosophila: a cdc2 homologue and string
EMBO J.
9
3565-3571
1990
Xenopus laevis (P23437)
Lehner, C.F.; O'Farrell, P.H.
Drosophila cdc2 homologul: a functional homolog is coexpressed with a cognate variant
EMBO J.
9
3573-3581
1990
Xenopus laevis (P23437), Drosophila melanogaster (P23573)
Fesquet, D.; Labbe, J.C.; Derancourt, J.; Capony, J.P.; Galas, S.; Girard, F.; Lorca, T.; Shuttleworth, J.; Doree, M.; Cavadore, J.C.
The MO15 gene encodes the catalytic subunit of a protein kinase that activates cdc2 and other cyclin-dependent kinases (CDKs) through phosphorylation of Thr161 and its homologues
EMBO J.
12
3111-3121
1993
Xenopus laevis (P24033), Xenopus laevis (P35567)
Pickham, K.M.; Meyer, A.N.; Li, J.; Donoghue, D.J.
Requirement of mosXe protein kinase for meiotic maturation of Xenopus oocytes induced by a cdc2 mutant lacking regulatory phosphorylation sites
Mol. Cell. Biol.
12
3192-3203
1992
Xenopus laevis (P24033), Xenopus laevis (P35567)
Gervasi, C.; Szaro, B.G.
The Xenopus laevis homologue to the neuronal cyclin-dependent kinase (cdk5) is expressed in embryos by gastrulation
Brain Res. Mol. Brain Res.
33
192-200
1995
Xenopus laevis (P51166), Xenopus laevis
D'Angiolella, V.; Mari, C.; Nocera, D.; Rametti, L.; Grieco, D.
The spindle checkpoint requires cyclin-dependent kinase activity
Genes Dev.
17
2520-2525
2003
Homo sapiens, Xenopus laevis
EXTERNAL LINKS (specific for EC-Number 2.7.11.22)
Transporter Classification Database (TCDB): 1.I.1.1.3
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