Any feedback?
Information on EC 2.7.11.21 - polo kinase and Organism(s) Schizosaccharomyces pombe and UniProt Accession P50528
for references in articles please use BRENDA:EC2.7.11.21Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
2.7.11.21 polo kinaseIUBMB Comments
The enzyme associates with the spindle pole during mitosis and is thought to play an important role in the dynamic function of the mitotic spindle during chromosome segregation. The human form of the enzyme, Plk1, does not phosphorylate histone H1, enolase and phosvitin but it can phosphorylate myelin basic protein and microtubule-associated protein MAP-2, although to a lesser extent than casein .
Specify your search results
Word Map
- 2.7.11.21
-
mitotic
- centrosome
-
checkpoint
-
cytokinesis
-
aurora
- microtubule
-
anaphase
- sirnas
-
polo-box
- cyclin
- kinetochore
-
cyclin-dependent
-
exit
- centriole
-
interphase
-
chromatid
-
metaphase
-
cell-cycle
-
meiosis
-
m-phase
-
faithful
-
aneuploidy
-
anaphase-promoting
-
pericentriolar
-
prometaphase
- cohesins
-
prophase
-
centromeres
- cdc25c
-
midbody
-
mitosis-specific
-
atp-competitive
- bubr1
-
kinetochore-microtubule
-
misalign
- midzone
- diagnostics
- pharmacology
-
missegregation
-
microtubule-organizing
-
monopolar
- securin
- wee1
- drug development
- cdc14
- analysis
-
condensin
-
metaphase-anaphase
- medicine
- gamma-tubulin
-
furrow
- mps1
- separase
- chk1
-
telophase
The taxonomic range for the selected organisms is: Schizosaccharomyces pombe
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
+
a [protein]-(L-serine/L-threonine)
=
+
a [protein]-(L-serine/L-threonine) phosphate
Synonyms
polo-like kinase 1, polo-like kinase, plk-1, polo kinase, polo-like kinase 4, polo-like kinase-1, tbplk, plk1 kinase, cdc5p, polo-like kinase 2, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
FGF-inducible kinase
-
-
-
-
Proliferation-related kinase
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (spindle-pole-dependent)
The enzyme associates with the spindle pole during mitosis and is thought to play an important role in the dynamic function of the mitotic spindle during chromosome segregation. The human form of the enzyme, Plk1, does not phosphorylate histone H1, enolase and phosvitin but it can phosphorylate myelin basic protein and microtubule-associated protein MAP-2, although to a lesser extent than casein [2].
CAS REGISTRY NUMBER
COMMENTARY
149433-93-2
Polo kinase
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + histone H1 kinase
ADP + phosphorylated histone H1 kinase
-
activation
-
-
?
ATP + Mid1p
ADP + phosphorylated Mid1p
-
required for positioning of division sites in cytokinesis
-
-
?
additional information
?
-
the enzyme is required to form a bipolar spindle, the actin ring, and septum, can drive septum formation in G1 and G2 cells
-
-
?
additional information
?
-
-
cytokinetic actomyosin ring formation and septation in fission yeast are dependent on the full recruitment of the polo-like kinase PLO1 to the spindle pole body, regulated by Mad2, and a functional spindle assembly checkpoint which requires an intact microtubule cytoskeleton, overview
-
-
?
additional information
?
-
-
physical and functional interactions between polo kinase and the spindle pole component Cut12 regulate mitotic commitment by feedback control of the MPF complex, overview
-
-
?
additional information
?
-
-
the enzyme is involved in spindle formation and septation playing an important role in cell cycle regulation
-
-
?
additional information
?
-
-
the enzyme is pivotal in cell division as a regulator of cell cycle checkpoints in mitotic progression, Plo1 is involved in cytokinesis
-
-
?
additional information
?
-
-
the enzyme recruitment to the spindle pole body influences the balance between Cdc25 and Wee1 activities on mitosis-promoting factor, MPF
-
-
?
additional information
?
-
-
the enzyme interacts with diverse proteins via its poloboxes, overview
-
-
?
additional information
?
-
-
the enzyme interacts with DNA polymerase delta
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + Mid1p
ADP + phosphorylated Mid1p
-
required for positioning of division sites in cytokinesis
-
-
?
additional information
?
-
the enzyme is required to form a bipolar spindle, the actin ring, and septum, can drive septum formation in G1 and G2 cells
-
-
?
additional information
?
-
-
cytokinetic actomyosin ring formation and septation in fission yeast are dependent on the full recruitment of the polo-like kinase PLO1 to the spindle pole body, regulated by Mad2, and a functional spindle assembly checkpoint which requires an intact microtubule cytoskeleton, overview
-
-
?
additional information
?
-
-
physical and functional interactions between polo kinase and the spindle pole component Cut12 regulate mitotic commitment by feedback control of the MPF complex, overview
-
-
?
additional information
?
-
-
the enzyme is involved in spindle formation and septation playing an important role in cell cycle regulation
-
-
?
additional information
?
-
-
the enzyme is pivotal in cell division as a regulator of cell cycle checkpoints in mitotic progression, Plo1 is involved in cytokinesis
-
-
?
additional information
?
-
-
the enzyme recruitment to the spindle pole body influences the balance between Cdc25 and Wee1 activities on mitosis-promoting factor, MPF
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
in vivo enzyme activity is dependent on Cut12
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
additional information
-
intracellular localization of wild-type and mutant enzymes, overview
-
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
Plo1 is a component of the spindle pole body, assembly at spindle checkpoint, mechanism, recruitment of Plo1 to the complex is inhibited by Mad2, overview
additional information
-
the enzyme contains 3 different polo box motifs in the non-catalytic region that are involved in protein protein interactions in the mitotic spindle formation process
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
phosphoprotein
-
Plo1 is phosphorylated at Ser402 in the stress response pathway, mechanism, phosphorylation is required for recruitment to the interphase spindle pole body and mitotic entry for cell tip growth and cell division, Plo1 phosphorylation is increased at elevated temperature
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
D181N
-
site-directed mutagenesis, mutation of the kinase domain, no complementation by plo1
D181R
-
site-directed mutagenesis, mutation of the kinase domain, no complementation by plo1
D623A/H624A/K625A
-
site-directed mutagenesis, mutation of polo box 3, mutant strain shows reduced mitotic activity, no complementation by plo1
E139K
-
site-directed mutagenesis, mutation in the kinase domain, mutant enzyme is fully active with histone H1 kinase
E193V
-
site-directed mutagenesis, mutation of the kinase domain, no complementation by plo1
F518A/N519A
-
site-directed mutagenesis, mutation of polo box 1, mutant strain shows reduced mitotic activity
G505A
-
site-directed mutagenesis, mutation of polo box 1, mutant strain shows reduced mitotic activity
K69R
-
site-directed mutagenesis, mutation of the kinase domain, mutant is catalytically inactive, no complementation by plo1
L577A
-
site-directed mutagenesis, mutation of polo box 2, mutant strain shows reduced mitotic activity
S402A
-
site-directed mutagenesis, no phosphorylation of the mutant by the stress response machinery, leading to delay in cell tip growth and cell division
T197V
-
site-directed mutagenesis, mutation of the kinase domain, no complementation by plo1
W49F
-
site-directed mutagenesis, mutation of polo box 1, mutant strain shows reduced mitotic activity
Y506A/Q507A/L508A
-
site-directed mutagenesis, mutation of polo box 1, mutant strain shows reduced mitotic activity, no complementation by plo1
additional information
additional information
-
construction of C-terminal deletions, the deletion mutants comprise residues 1-633, 1-583, and 1-483, no complementation of the mutants by plo1
additional information
-
generation of temperature-sensitive mutants of Plo1 which are inactivated at 35°C for 4 h, Plo1 activity is abolished in Cut12 loss-of-function mutant cells, but is increased in Cut12 gain-of-function mutant cells
additional information
-
overexpression of Plo1 results in formation of multiple septa without nuclear division
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overexpression of mutant K69R in a checkpoint-defective mad2-deletion strain, two-hybrid expression of wild-type and mutant enzymes with diverse protein partners for interaction study, overview
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wood, V.; Gwilliam, R.; Rajandream, M.A.; Lyne, M.; Lyne, R.; et al.
The genome sequence of Schizosaccharomyces pombe
Nature
415
871-880
2002
Schizosaccharomyces pombe (P50528)
Ohkura, H.; Hagan, I.M.; Glover, D.M.
The conserved Schizosaccharomyces pombe kinase plo1, required to form a bipolar spindle, the actin ring, and septum, can drive septum formation in G1 and G2 cells
Genes Dev.
9
1059-1073
1995
Schizosaccharomyces pombe (P50528)
MacIver, F.H.; Tanaka, K.; Robertson, A.M.; Hagan, I.M.
Physical and functional interactions between polo kinase and the spindle pole component Cut12 regulate mitotic commitment in S. pombe
Genes Dev.
17
1507-1523
2003
Schizosaccharomyces pombe
Mulvihill, D.P.; Hyams, J.S.
Cytokinetic actomyosin ring formation and septation in fission yeast are dependent on the full recruitment of the polo-like kinase PLO1 to the spindle pole body and a functional spindle assembly checkpoint
J. Cell Sci.
115
3575-3586
2002
Schizosaccharomyces pombe
Reynolds, N.; Ohkura, H.
Polo boxes form a single functional domain that mediates interactions with multiple proteins in fission yeast polo kinase
J. Cell Sci.
116
1377-1387
2003
Schizosaccharomyces pombe
Petersen, J.; Hagan, I.M.
Polo kinase links the stress pathway to cell cycle control and tip growth in fission yeast
Nature
435
507-512
2005
Schizosaccharomyces pombe
EXTERNAL LINKS (specific for EC-Number 2.7.11.21)
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
