Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.7.11.18 - myosin-light-chain kinase and Organism(s) Dictyostelium discoideum and UniProt Accession P25323

for references in articles please use BRENDA:EC2.7.11.18
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
Requires Ca2+ and calmodulin for activity. The 20-kDa light chain from smooth muscle myosin is phosphorylated more rapidly than any other acceptor, but light chains from other myosins and myosin itself can act as acceptors, but more slowly.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Dictyostelium discoideum
UNIPROT: P25323
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Dictyostelium discoideum
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
mlck, myosin light chain kinase, myosin light-chain kinase, mlc kinase, smooth muscle myosin light chain kinase, smmlck, nmmlck, myosin kinase, cmlck, skmlck, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
myosin light chain kinase
-
myosin light chain kinase A
-
calcium/calmodulin-dependent myosin light chain kinase
-
-
-
-
kinase, myosin light-chain (phosphorylating)
-
-
-
-
myosin kinase
-
-
-
-
myosin light chain protein kinase
-
-
-
-
myosin light-chain kinase
-
-
-
-
smooth-muscle-myosin-light-chain kinase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:[myosin light chain] O-phosphotransferase
Requires Ca2+ and calmodulin for activity. The 20-kDa light chain from smooth muscle myosin is phosphorylated more rapidly than any other acceptor, but light chains from other myosins and myosin itself can act as acceptors, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
51845-53-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + Dictyostelium myosin
ADP + Dictyostelium myosin phosphate
show the reaction diagram
-
-
-
?
ATP + kemptamide
ADP + kemptamide phosphate
show the reaction diagram
i.e. peptide KKRPQRATSNVFS-NH2, activated enzyme
-
-
?
ATP + myosin
ADP + myosin phosphate
show the reaction diagram
-
-
-
?
ATP + myosin II
ADP + myosin II phosphate
show the reaction diagram
from Dictyostelium discoideum, activated enzyme
-
-
?
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
ATP + protein
ADP + phosphoprotein
show the reaction diagram
intramolecular autophosphorylation activates the enzyme
-
-
?
ATP + [myosin II light chain]
ADP + [myosin II lihght chain] phosphate
show the reaction diagram
from Dictyostelium discoideum, activated enzyme
-
-
?
additional information
?
-
skeletal, gizzard smooth and cardiac enzymes perform intramolecular autophosphorylation in the absence of acceptor substrate
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + myosin light chain
ADP + myosin light chain phosphate
show the reaction diagram
-
-
-
?
ATP + myosin light chain
ADP + phosphorylated myosin light chain
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Increasing ionic strength
above 0.1 M KCl
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
autophosphorylation
activation
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.004
Dictyostelium myosin
22°C, pH 7.5
-
0.004
myosin
myosin from Dictyostelium
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
MYLKA_DICDI
295
0
33407
Swiss-Prot
other Location (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
1 * 34000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
the enzyme is 140fold activated through phosphorylation at Thr166 by a recombinant constitutively active Ca2+/calmodulin-dependent protein kinase kinase, the enzyme performs autophosphorylation at Thr289, subsequent to Thr166 phosphorylation by the protein kinase kinase, and at several serine residues, leading to release of the autoinhibitory domain from the catalytic core, identification of phosphorylation sites
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
the full-length enzyme as well as a truncated form lacking the putative auto-inhibitory domain are expressed in bacterial cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Tan, J.L.; Spudich, J.A.
Characterization and bacterial expression of the Dictyostelium myosin light chain kinase cDNA. Identification of an autoinhibitory domain
J. Biol. Chem.
266
16044-16049
1991
Dictyostelium discoideum (P25323)
Manually annotated by BRENDA team
Tan, J.L.; Spudich, J.A.
Dictyostelium myosin light chain kinase. Purification and characterization
J. Biol. Chem.
265
13818-13824
1990
Dictyostelium discoideum (P25323), Dictyostelium discoideum Ax-3 (P25323)
Manually annotated by BRENDA team
Tokumitsu, H.; Hatano, N.; Inuzuka, H.; Ishikawa, Y.; Uyeda, T.Q.; Smith, J.L.; Kobayashi, R.
Regulatory mechanism of Dictyostelium myosin light chain kinase A
J. Biol. Chem.
279
42-50
2004
Dictyostelium discoideum (P25323)
Manually annotated by BRENDA team