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Information on EC 2.7.11.13 - protein kinase C and Organism(s) Mus musculus and UniProt Accession P20444

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IUBMB Comments
A family of serine- and threonine-specific protein kinases that depend on lipids for activity. They can be activated by calcium but have a requirement for the second messenger diacylglycerol. Members of this group of enzymes phosphorylate a wide variety of protein targets and are known to be involved in diverse cell-signalling pathways. Members of the protein kinase C family also serve as major receptors for phorbol esters, a class of tumour promoters.
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Mus musculus
UNIPROT: P20444
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
+
a [protein]-(L-serine/L-threonine)
=
+
a [protein]-(L-serine/L-threonine) phosphate
Synonyms
protein kinase c, pkcalpha, pkc-alpha, pkc alpha, pkcepsilon, pkc-delta, pkczeta, pkc-epsilon, pkc delta, pkc-zeta, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alpha-PKC
-
PKCalpha
protein kinase C, alpha type
-
AS PKCdelta
-
-
ATP analog specific protein kinase Cdelta
-
-
Calcium-dependent protein kinase C
-
-
-
-
epsilonPKC
-
-
-
-
PKC deltaII
-
PKC lambda
-
PKC-beta1
-
-
PKC-epsilon
-
PKCbeta1
isozyme
PKCbeta2
isozyme
PKCdelta
isozyme
PKCepsilon
PKCgamma
PKCthetaC1B
-
C1B subdomain
protein kinase C
protein kinase C delta
-
protein kinase C, delta type
-
protein kinase C, epsilon type
-
protein kinase C, eta type
-
protein kinase C, gamma type
-
protein kinase C, iota type
-
protein kinase C, zeta type
-
protein kinase Cdelta
-
protein kinase-C
-
-
-
-
additional information
SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (diacylglycerol-dependent)
A family of serine- and threonine-specific protein kinases that depend on lipids for activity. They can be activated by calcium but have a requirement for the second messenger diacylglycerol. Members of this group of enzymes phosphorylate a wide variety of protein targets and are known to be involved in diverse cell-signalling pathways. Members of the protein kinase C family also serve as major receptors for phorbol esters, a class of tumour promoters.
CAS REGISTRY NUMBER
COMMENTARY hide
141436-78-4
calcium-dependent protein kinase C
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
-
-
?
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
ATP + a protein
ADP + phosphorylated protein
show the reaction diagram
-
-
-
-
?
ATP + Akt
ADP + phosphorylated Akt
show the reaction diagram
ATP + Bcl-xL
ADP + phosphorylated Bcl-xL
show the reaction diagram
-
recombinant human substrate, recombinant isozymes PKCepsilon and PKCzeta
-
-
?
ATP + Btk
ADP + phosphorylated Btk
show the reaction diagram
ATP + GluR6 kainate receptor subunit
ADP + phosphorylated GluR6 kainate receptor subunit
show the reaction diagram
-
two specific residues on the GluR6 C terminus, Ser846 and Ser868, are phosphorylated by protein kinase C, PKC phosphorylation of GluR6 retains GluR6 in the endoplasmic reticulum
-
-
?
ATP + insulin receptor
ADP + phosphorylated insulin receptor
show the reaction diagram
-
isozyme PKCalpha
-
-
?
ATP + IRS
ADP + phosphorylated IRS
show the reaction diagram
-
isozymes PKCalpha and PKCdelta, phosphorylation on Ser307
-
-
?
ATP + myristoylated alanine-rich C kinase substrate
ADP + phosphorylated myristoylated alanine-rich C kinase substrate
show the reaction diagram
ATP + Par-3
ADP + phosphorylated Par-3
show the reaction diagram
-
-
-
?
ATP + phosphoinositide-dependent kinase
ADP + phosphorylated phosphoinositide-dependent kinase
show the reaction diagram
ATP + poly(ADP-ribose) polymerase-1
ADP + phosphorylated poly(ADP-ribose) polymerase-1
show the reaction diagram
-
-
-
-
?
ATP + protein
ADP + phosphoprotein
show the reaction diagram
autophosphorylation
-
-
?
ATP + transient receptor potential vanilloid 5
ADP + phosphorylated transient receptor potential vanilloid 5
show the reaction diagram
-
-
-
-
?
ATP + vasodilator-stimulated phosphoprotein
ADP + phosphorylated vasodilator-stimulated phosphoprotein
show the reaction diagram
-
phosphorylation at Ser157
-
-
?
Nepsilon-(benzyl)-ATP + histone 3
ADP + phosphorylated histone 3
show the reaction diagram
-
-
-
-
?
Nepsilon-(benzyl)-ATP + RFARKGSLRQKNV
Nepsilon-(benzyl)-ADP + RFARKGpSLRQKNV
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
-
-
?
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
ATP + a protein
ADP + phosphorylated protein
show the reaction diagram
-
-
-
-
?
ATP + Akt
ADP + phosphorylated Akt
show the reaction diagram
-
phosphorylation by PKC isozymes at Ser473, regulation of the phosphorylation activity by PKCbetaII in mast cells stimulated by stem cell factor or interaleukin-3, and in serum-stimulated fibroblasts, and in antigen-receptor stimulated T- or B-lymphocytes, overview
-
-
?
ATP + Btk
ADP + phosphorylated Btk
show the reaction diagram
-
specific phosphorylation leads to inhibition of Btk membrane translocation and activation and the downstream events that promote PKCbeta activation, mechanism, overview
-
-
?
ATP + myristoylated alanine-rich C kinase substrate
ADP + phosphorylated myristoylated alanine-rich C kinase substrate
show the reaction diagram
-
the substrate is involved in actincytoskeletal rearrangement in response to extra-cellular stimuli, phosphorylation of MARCKS is dramatically upregulated specifically in microglial cells after kainic acid-induced seizures, but not in other types of glial cells, overview
-
-
?
ATP + phosphoinositide-dependent kinase
ADP + phosphorylated phosphoinositide-dependent kinase
show the reaction diagram
-
phosphoinositide-dependent kinases are conserved substrates of PKC
-
-
?
ATP + vasodilator-stimulated phosphoprotein
ADP + phosphorylated vasodilator-stimulated phosphoprotein
show the reaction diagram
-
phosphorylation at Ser157
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
GF109203X
bisindolylmaleimide I
1-(5-isoquinolinesulfonyl)-2-methylpiperazine
-
1-(tert-butyl)-3-(1-naphthyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine
-
inhibits ATP analog-specific PKCdelta isoform
1-(tert-butyl)-3-(2-methylbenzyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine
-
inhibits ATP analog-specific PKCdelta isoform
angiotensin II type 2-receptor
-
inhibits isozyme PKCalpha
-
bisindolylmaleimide derivatives
-
-
bisindolylmaleimide I
-
-
CG53353
-
inhibitor of isozyme PKCbetaII
GF109203X
Go6976
Go6983
-
-
G├Â6976
-
inhibits classical isozymes
quinolinic acid
-
inhibits the phosphorylation of the enzyme
rottlerin
staurosporine
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,2-dioctanoyl-sn-glycerol
-
-
1-oleoyl-acetyl-sn-glycerol
-
0.01 mM
1-stearoyl-2-arachidonoyl-sn-glycerol
-
-
12-O-Tetradecanoylphorbol 13-acetate
-
activates isozyme PKCepsilon
13-O-acetylphorbol 12-myristate
-
-
Berberine
-
-
diacylglycerol
Diacylglycerols
-
activate PKC isozymes
fatty acids
-
activation mechanism
IGF-1
-
-
-
Insulin
-
activates PKC isozymes alpha, betaII, delta, and zeta in several cell types, activation mechanism
-
Kainic acid
-
phosphorylation of MARCKS by PKC isozymes is dramatically upregulated specifically in microglial cells after kainic acid-induced seizures, but not in other types of glial cells, overview, upregulation of isozymes PKCalpha, PKCbetaI, PKCbetaII, and PKCdelta
phorbol 12,13-dibutyrate
-
-
phorbol 12-myristate 13-acetate
phorbol dibutyrate
activates
phosphatidylserine
-
activation mechanism
Phospholipid
strict dependence on the presence of phospholipids
Phospholipids
-
regulatory function for isozymes alpha, betaI, betaII, gamma, delta, epsilon, eta, and theta
thymeleatoxin
-
activates Ca2+-dependent PKC isozymes, reversal by chronic thymeleatoxin pretreatment of the effects of carbachol on glucose-induced Ca2+ oscillations and pulsatile 5-HT release, overview
additional information
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000154
1-(tert-butyl)-3-(1-naphthyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine
Mus musculus
-
ATP analog-specific PKCdelta isoform, at pH 7.4 and 27┬░C
0.0026
1-(tert-butyl)-3-(2-methylbenzyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine
Mus musculus
-
ATP analog-specific PKCdelta isoform, at pH 7.4 and 27┬░C
0.000221
bisindolylmaleimide I
Mus musculus
-
ATP analog-specific PKCdelta isoform, at pH 7.4 and 27┬░C
0.00167
staurosporine
Mus musculus
-
ATP analog-specific PKCdelta isoform, at pH 7.4 and 27┬░C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
assay at
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
the PKCalpha is expressed prominently in the retinal growth cones, while abundant in the filopodial and lamellipodial processes
Manually annotated by BRENDA team