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Information on EC 2.7.11.11 - cAMP-dependent protein kinase and Organism(s) Homo sapiens and UniProt Accession P22612

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IUBMB Comments
cAMP is required to activate this enzyme. The inactive holoenzyme of cAMP-dependent protein kinase is a tetramer composed of two regulatory (R) and two catalytic (C) subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP molecules and two free monomeric catalytic subunits [i.e. R2C2 + 4 cAMP = R2(cAMP)4 + 2 C].
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Select one or more organisms in this record:
This record set is specific for:
Homo sapiens
UNIPROT: P22612
Word Map
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
The taxonomic range for the selected organisms is: Homo sapiens
Reaction Schemes
Synonyms
cAMP dependent protein kinase, cAMP-dependent protein kinase, cAMP-dependent protein kinase A, cAMP-dependent protein kinase catalytic subunit, cAMP-dependent protein kinase catalytic subunit (C-subunit), cAMP-dependent protein kinase type 1, cAMP-dependent protein kinase type 2, cAMP-dependent protein kinase type 3, cAMP-dependent protein kinase type I, cAMP-dependent protein kinase, alpha-catalytic subunit, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cAMP-dependent protein kinase
cAMP-dependent protein kinase A
247
-
cAMP-dependent protein kinase, alpha-catalytic subunit
265790
-
cAMP-dependent protein kinase, beta-catalytic subunit
265795
-
cAMP-dependent protein kinase, gamma-catalytic subunit
265794
-
cAMP/protein kinase A
247
-
cyclic AMP-dependent protein kinase
247
-
cyclic AMP-dependent protein kinase A
247
-
PK-25
-
-
-
-
PKA C-alpha
-
-
-
-
PKA C-beta
-
-
-
-
PKA C-gamma
-
-
-
-
PKA RIalpha
247
regulatory subunit I alpha of PKA
PKA type I
247
isoform
PKA type II
PKA-I
247
isozyme
PKA-II
247
isozyme
PKAc
306132
catalytic subunit
PRKACA
265790
catalytic subunit
protein kinase A type II
247
-
protein kinase-A
247
-
type I protein kinase A
247
-
type II protein kinase A
247
-
additional information
247
see also EC 2.7.11.26
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + a protein = ADP + a phosphoprotein
show the reaction diagram
catalytic mechanism
-
SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (cAMP-dependent)
cAMP is required to activate this enzyme. The inactive holoenzyme of cAMP-dependent protein kinase is a tetramer composed of two regulatory (R) and two catalytic (C) subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP molecules and two free monomeric catalytic subunits [i.e. R2C2 + 4 cAMP = R2(cAMP)4 + 2 C].
CAS REGISTRY NUMBER
COMMENTARY hide
142008-29-5
-
142008-29-5
cAMP-dependent protein kinase
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + AID protein
ADP + phosphorylated AID protein
show the reaction diagram
-
-
-
-
?
ATP + alpha7 nicotinic acetylcholine receptor
ADP + phosphorylated alpha7 nicotinic acetylcholine receptor
show the reaction diagram
-
phosphorylation at Ser365
-
-
?
ATP + bAD protein
ADP + phosphorylated bAD protein
show the reaction diagram
-
-
-
-
?
ATP + beta-adrenergic receptor
ADP + phosphorylated beta-adrenergic receptor
show the reaction diagram
ATP + beta1-adrenergic receptor
ADP + phosphorylated beta1-adrenergic receptor
show the reaction diagram
ATP + BKCa channel ZERO
ADP + phosphorylated BKCa channel ZERO
show the reaction diagram
ATP + c-Myc protein
ADP + phosphorylated c-Myc protein
show the reaction diagram
-
-
-
-
?
ATP + calpain 2
ADP + phosphorylated calpain 2
show the reaction diagram
-
-
-
-
?
ATP + carboxyl-terminal binding protein 1
ADP + phosphorylated carboxyl-terminal binding protein 1
show the reaction diagram
-
-
-
-
?
ATP + CFTR protein
ADP + phosphorylated CFTR protein
show the reaction diagram
-
-
-
-
?
ATP + claudin-3
ADP + phosphorylated claudin-3
show the reaction diagram
ATP + claudin-3 S199A
ADP + phosphorylated claudin-3 S199A
show the reaction diagram
-
recombinant GST-fusion claudin-3 mutant S199A expressed in Escherichia coli, phosphorylation at Thr192, activity of catalytic PKA subunit is lower compared to wild-type claudin-3
-
-
?
ATP + CREb protein
ADP + phosphorylated CREb protein
show the reaction diagram
-
-
-
-
?
ATP + G protein-coupled receptor GRK1
ADP + phosphorylated G protein-coupled receptor GRK1
show the reaction diagram
ATP + G protein-coupled receptor GRK7
ADP + phosphorylated G protein-coupled receptor GRK7
show the reaction diagram
ATP + glycogen synthase kinase 3beta
ADP + phosphorylated glycogen synthase kinase 3beta
show the reaction diagram
-
phosphorylation of glycogen synthase kinase 3beta is carried out by the PKA type II isoform
-
-
?
ATP + GRK2 protein
ADP + phosphorylated GRK2 protein
show the reaction diagram
-
-
-
-
?
ATP + GSK3 protein
ADP + phosphorylated GSK3 protein
show the reaction diagram
-
-
-
-
?
ATP + histone
ADP + phosphorylated histone
show the reaction diagram
-
-
-
-
?
ATP + histone 3s
ADP + phosphorylated histone 3s
show the reaction diagram
-
activity of the catalytic subunit Cgamma
-
-
?
ATP + histone deacetylase 8
ADP + phosphorylated histone deacetylase 8
show the reaction diagram
ATP + HSL protein
ADP + phosphorylated HSL protein
show the reaction diagram
-
-
-
-
?
ATP + KCNN2 protein
ADP + phosphorylated KCNN2 protein
show the reaction diagram
-
-
-
-
?
ATP + Kemptide
ADP + Kemptide phosphate
show the reaction diagram
ATP + kemptide
ADP + phospho-kemptide
show the reaction diagram
-
-
-
-
?
ATP + Kemptide
ADP + phosphorylated Kemptide
show the reaction diagram
ATP + LRRASLG
ADP + LRRA-phosphoserine-LG
show the reaction diagram
-
i.e. Kemptide, recombinant PKA catalytic subunit
-
-
?
ATP + merlin
ADP + phosphorylated merlin
show the reaction diagram
ATP + mitogen-activated protein kinase phosphatase-1
ADP + phosphorylated mitogen-activated protein kinase phosphatase-1
show the reaction diagram
ATP + NDUFS4 subunit of complex I
ADP + phosphorylated NDUFS4 subunit of complex I
show the reaction diagram
-
complex I is the NADH-ubiquinone oxidoreductase, E.C 1.6.5.3
-
-
?
ATP + NFAT2 protein
ADP + phosphorylated NFAT2 protein
show the reaction diagram
-
-
-
-
?
ATP + PKA regulatory subunit IIalpha
ADP + phosphorylated PKA regulatory subunit IIalpha
show the reaction diagram
-
-
-
-
?
ATP + protein tyrosine phosphatase
ADP + phosphorylated protein tyrosine phosphatase
show the reaction diagram
-
i.e. PTP, contains a PEST motif
-
-
?
ATP + RFARKGSLREKNV
ADP + RFARKG-phosphoserine-LREKNV
show the reaction diagram
-
protein kinase C-derived peptide, activity of the catalytic subunit Cgamma
-
-
?
ATP + RKRSRAE
ADP + RKR-phosphoserine-RAE
show the reaction diagram
-
cGPK-1-derived peptide, activity of the catalytic subunit Cgamma
-
-
?
ATP + RKRSRKE
ADP + RKR-phosphoserine-RKE
show the reaction diagram
-
cGPK-2-derived peptide, activity of the catalytic subunit Cgamma
-
-
?
ATP + RRLSSLRA
ADP + RRL-phosphoserine-phosphoserine-LRA
show the reaction diagram
-
S6 kinase-derived peptide, activity of the catalytic subunit Cgamma
-
-
?
ATP + small heat shock protein HSP22
ADP + phosphorylated small heat shock protein HSP22
show the reaction diagram
-
-
-
-
?
ATP + SP20 protein
ADP + phosphorylated SP20 protein
show the reaction diagram
i.e. TTYADFIASGRTGRRASIHD
-
-
?
ATP + type III inositol 1,4,5-trisphosphate receptor
ADP + phosphorylated type III inositol 1,4,5-trisphosphate receptor
show the reaction diagram
ATP + tyrosine hydroxylase
ADP + phosphorylated tyrosine hydroxylase
show the reaction diagram
-
activity of catalytic PKA subunit
-
-
?
ATP + VASP
ADP + phosphorylated VASP
show the reaction diagram
-
i.e. vasodilator-stimulated phosphoprotein
-
-
?
ATP + VASP protein
ADP + phosphorylated VASP protein
show the reaction diagram
-
-
-
-
?
ATP + [tau-protein]
ADP + O-phospho-[tau-protein]
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + AID protein
ADP + phosphorylated AID protein
show the reaction diagram
-
-
-
-
?
ATP + alpha7 nicotinic acetylcholine receptor
ADP + phosphorylated alpha7 nicotinic acetylcholine receptor
show the reaction diagram
-
phosphorylation at Ser365
-
-
?
ATP + bAD protein
ADP + phosphorylated bAD protein
show the reaction diagram
-
-
-
-
?
ATP + beta-adrenergic receptor
ADP + phosphorylated beta-adrenergic receptor
show the reaction diagram
-
PKA phosphorylation mediates beta-1 adrenergic receptor endocytosis via the caveolae pathway
-
-
?
ATP + beta1-adrenergic receptor
ADP + phosphorylated beta1-adrenergic receptor
show the reaction diagram
-
recombinant beta1-adrenergic receptor expressed in HEK-293 cell membranes, phosphorylation at Ser312 is essential for activation of endocytic recycling of the agonist-internalized beta1-adrenergic receptor, beta1-AR mutant S312A is not recycled, overview
-
-
?
ATP + BKCa channel ZERO
ADP + phosphorylated BKCa channel ZERO
show the reaction diagram
-
recombinant murine HA-tagged tetrameric protein expressed in HEK-293 cells, activity with wild-type and mutant Y334V channel tetramers, but no activity with S899A mutant, phosphorylation at Ser899 activates the channels, overview
-
-
?
ATP + c-Myc protein
ADP + phosphorylated c-Myc protein
show the reaction diagram
-
-
-
-
?
ATP + calpain 2
ADP + phosphorylated calpain 2
show the reaction diagram
-
-
-
-
?
ATP + CFTR protein
ADP + phosphorylated CFTR protein
show the reaction diagram
-
-
-
-
?
ATP + claudin-3
ADP + phosphorylated claudin-3
show the reaction diagram
-
high activity in ovarian cancer cells with recombinantly overexpressed claudin-3, phosphorylation of claudin-3 affecting the barrier function with extracellular Ca2+, overview
-
-
?
ATP + CREb protein
ADP + phosphorylated CREb protein
show the reaction diagram
-
-
-
-
?
ATP + G protein-coupled receptor GRK1
ADP + phosphorylated G protein-coupled receptor GRK1
show the reaction diagram
-
recombinant FLAG-tagged GRK1 expressed in HEK-293 cells, enzymatic inactivation of the receptor activity inhibiting rhodopsin phosphorylation
-
-
?
ATP + G protein-coupled receptor GRK7
ADP + phosphorylated G protein-coupled receptor GRK7
show the reaction diagram
-
recombinant FLAG-tagged GRK1 expressed in HEK-293 cells, enzymatic inactivation of the receptor activity inhibiting rhodopsin phosphorylation
-
-
?
ATP + GRK2 protein
ADP + phosphorylated GRK2 protein
show the reaction diagram
-
-
-
-
?
ATP + GSK3 protein
ADP + phosphorylated GSK3 protein
show the reaction diagram
-
-
-
-
?
ATP + histone deacetylase 8
ADP + phosphorylated histone deacetylase 8
show the reaction diagram
-
FLAG-tagged class 1 histone deacetylase HDAC8 expressed in HeLa cells via adenovirus infection, phosphorylation at Ser39 reduces the enzyme activity of HDAC8, hyperphosphorylation inhibits the enzyme
-
-
?
ATP + HSL protein
ADP + phosphorylated HSL protein
show the reaction diagram
-
-
-
-
?
ATP + KCNN2 protein
ADP + phosphorylated KCNN2 protein
show the reaction diagram
-
-
-
-
?
ATP + merlin
ADP + phosphorylated merlin
show the reaction diagram
-
recombinant human substrate protein expressed in HEK-293 cells, activity of the catalytic subunit C, phosphorylation at Ser518 induces N-terminal binding of merlin to ezrin
-
-
?
ATP + mitogen-activated protein kinase phosphatase-1
ADP + phosphorylated mitogen-activated protein kinase phosphatase-1
show the reaction diagram
-
the enzyme enhances steroid hydroxylase CYP17 transcription via mitogen-activated protein kinase phosphatase-1 MKP-1 activation in H295R adrenocortical cells
-
-
?
ATP + NDUFS4 subunit of complex I
ADP + phosphorylated NDUFS4 subunit of complex I
show the reaction diagram
-
complex I is the NADH-ubiquinone oxidoreductase, E.C 1.6.5.3
-
-
?
ATP + NFAT2 protein
ADP + phosphorylated NFAT2 protein
show the reaction diagram
-
-
-
-
?
ATP + SP20 protein
ADP + phosphorylated SP20 protein
show the reaction diagram
P61925
i.e. TTYADFIASGRTGRRASIHD
-
-
?
ATP + type III inositol 1,4,5-trisphosphate receptor
ADP + phosphorylated type III inositol 1,4,5-trisphosphate receptor
show the reaction diagram
-
i.e. IP3 receptor, recombinantly expressed in HEK cells, forms tetrameric Ca2+ channels in the endoplasmic reticulum
-
-
?
ATP + VASP protein
ADP + phosphorylated VASP protein
show the reaction diagram
-
-
-
-
?
ATP + [tau-protein]
ADP + O-phospho-[tau-protein]
show the reaction diagram
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cAMP
dependent on
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
-
-
additional information
-
in HeLa cells PKA activity follows a biphasic response to thiol oxidation, overview
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
AdcAhxArg6
-
i.e. adenosine 5'-carboxylic acid-6-aminohexanoic acid-L-arginine, peptide-nucleoside conjugate inhibitor, inhibition of the catalytic subunit, binding mechanism
adenosine 3',5'-cyclic phosphorothioate
-
-
beta,gamma-methyleneadenosine 5'-triphosphate
-
KT5720
-
-
myristoylated PKI
-
specific inhibitor
-
myristoylated protein kinase I (14-22) amide
-
specific PKA inhibitor
-
PKA regulatory subunit Ialpha
-
pseudosubstrate inhibitor
-
PKI
-
protein kinase inhibitor peptide
-
PKI
-
pseudosubstrate peptides
-
IC50 values for inhibition of the regulatory subunits of PKA by endogenous inhibitors, overview
-
RIIalpha subunit
-
-
-
STVHEILCKLSLEG
-
an acetylated peptide Ac1-14, but not the nonacetylated equivalent N1-14, inhibits PKA-dependent outwardly rectifying Cl- channels, ORCC, and CFTRmediated currents, the peptide sequence is equivalent to the S100A10 binding site on anx 2, it disrupts the anx S100A10/CTFR interaction
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8-bromo-cAMP
8-CPT-cAMP
-
induces release of the active catalytic subunits from the PKA holoenzyme
Adrenocorticotropin
-
induces PKA activation
-
Calcineurin
-
i.e. CaN, is important for the cAMP/PKA-dependent anx 2S100A10 complex formation
-
cAMP-response element-binding protein-binding protein/p300
-
PKA coactivator
-
dibutyryl-cAMP
-
induces MKP-1 mRNA and protein expression in H295R cells
forskolin
isoproterenol
-
stimulates
N6,2'-O-dibutyryladenosine 3',5'-cyclic phosphate
-
induces PKA activation
prostaglandin E1
-
activates
prostaglandin E2
-
0.05 mM induce activity by elevation of cAMP levels
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0125
Kemptide
-
pH 7.0, 30C, recombinant catalytic subunit Cgamma
0.0253
RFARKGSLREKNV
-
pH 7.0, 30C, recombinant catalytic subunit Cgamma
0.0333
RKRSRAE
-
pH 7.0, 30C, recombinant catalytic subunit Cgamma
0.0333
RKRSRKE
-
pH 7.0, 30C, recombinant catalytic subunit Cgamma
0.0503
RRLSSLRA
-
pH 7.0, 30C, recombinant catalytic subunit Cgamma
additional information
additional information
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00013
AdcAhxArg6
-
pH 7.5, 30C, recombinant catalytic subunit
additional information
additional information
-
inhibition kinetics for the catalytic subunit
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.31
-
purified recombinant catalytic subunit Cgamma, substrate Kemptide
0.81
-
purified recombinant catalytic subunit Cgamma, substrate histone
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
-
assay at
7
-
assay at
7.2
-
assay at
7.5
-
assay at
7.6
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
mesenchymal cell
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
HEK-293 cells that stably express D1 receptors
Manually annotated by BRENDA team
-
nasal
Manually annotated by BRENDA team
-
adut skin and NHDF neonatal human dermal fibroblasts
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
immortilized ovarian surface epithelial cell line
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
from sickle cell disease patients
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
fetal lung fibroblast-like cell line
Manually annotated by BRENDA team
additional information
-
high PKA activity in ovarian cancer cells
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
cAMP/PKA-dependent anx 2S100A10 complex
Manually annotated by BRENDA team
additional information
-
the enzyme binds to and colocalizes with the kinase-anchoring protein gravin in the neuronal cortex membrane which is enriched in the inner peripheral cortex in close proximity to the plasma membrane
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
combined inhibition of the androgen receptor and the regulatory subunit I alpha of PKA with small interference RNAs significantly increases the growth-inhibitory and proapoptotic effects of androgen receptor knockdown. Downregulating PKA RIalpha is sufficient to inhibit PKA signaling and also impairs androgen receptor expression and activation. Depletion of PKA RIalpha also potentiates the antiproliferative effect of the antiandrogen bicalutamide in androgen-sensitive LNCaP cells
physiological function
additional information
-
NDUFS4 mutations result in defective assembly of complex I with severe effects on the respiratory chain, mouse model NDUFS4 point mutations and phenotypes, overview
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
KAPCG_HUMAN
351
0
40434
Swiss-Prot
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41000
-
1 * 41000, isolated recombinant catalytic subunit Cgamma from Sf9 cells, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
oligomer
-
-
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
-
activation loop phosphorylation at Thr197 regulates the enzyme's catalytic activity, molecular mechanism, classical molecular dynamics simulations and ab initio QM/MM calculations are carried out on the wild-type PKA-Mg2+- ATP-substrate complex and its dephosphorylated mutant, T197A, overview
additional information
-
unmyristylated Calpha2 may be essential for fertility in the male
CRYSTALLIZATION/commentary
ORGANISM
UNIPROT
LITERATURE
in complex with Mg2+ and beta,gamma-methyleneadenosine 5'-triphosphate, sitting drop vapor diffusion method, using 100 mM MES (pH 6.5), 5 mM dithiothreitol, and 15-20% (w/v) PEG 4000 at 4-14C
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R165A
-
site-directed mutagenesis, the mutant cannot be phosphorylated at Thr197 of the activation loop, molecular dynamics simulations, overview
T197A
-
site-directed mutagenesis, the side-chain conformations of the P-site Ser in the R165A mutant are similar to that in the wild-type PKA, although the replacement of Arg165 with Ala disconnects the interactions between the pThr 197 and the active site, molecular dynamics simulations, overview
additional information
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
N-terminal and C-terminal extensions stabilize the catalytic core
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70C, purified recombinant catalytic subunit Cgamma, 0.5 M NaCl, 10 mM potassium phosphate, pH 6.9, 1 mM EDTA, 30% glycerol, on freeze-thaw cycle, stable up to one year
-
PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
HisTrap column chromatography
recombinant His-tagged catalytic subunit Cgamma from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant catalytic subunit Cgamma 273fold from Sf9 insect cells by adsorption chromatography and gel filtration
-
CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Neuro2a cells
-
expression of His-tagged catalytic subunit Cgamma in Escherichia coli strain BL21(DE3), stable expression of catalytic subunit Cgamma in cAMP-resistant mouse Y1 adrenal mutant cell line, expression of catalytic subunit Cgamma in Spodoptera frugiperda Sf9 cells via baculovirus infection system
-
expression of the catalytic subunit C in HEK-293 cells
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
hepatitis C virus infection activates PKA in a cAMP-dependent manner to promote virus release and transmission
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Agustin, J.T.; Wilkerson, C.G.; Witman, G.B.
The unique catalytic subunit of sperm cAMP-dependent protein kinase is the product of an alternative Calpha mRNA expressed specifically in spermatogenic cells
Mol. Biol. Cell
11
3031-3044
2000
Homo sapiens, Homo sapiens (P17612)
Manually annotated by BRENDA team
Desseyn, J.L.; Burton, K.A.; McKnight, G.S.
Expression of a nonmyristylated variant of the catalytic subunit of protein kinase A during male germ-cell development
Proc. Natl. Acad. Sci. USA
97
6433-6438
2000
Homo sapiens (P17612)
Manually annotated by BRENDA team
Maldonado, F.; Hanks, S.K.
A cDNA clone encoding human cAMP-dependent protein kinase catalytic subunit C alpha
Nucleic Acids Res.
16
8189-8190
1988
Homo sapiens, Homo sapiens (P17612)
Manually annotated by BRENDA team
Beebe, S.J.; Oyen, O.; Sandberg, M.; Froysa, A.; Hansson, V.; Jahnsen, T.
Molecular cloning of a tissue-specific protein kinase (C gamma) from human testis--representing a third isoform for the catalytic subunit of cAMP-dependent protein kinase
Mol. Endocrinol.
4
465-475
1990
Homo sapiens, Homo sapiens (P22612), Homo sapiens (P22694)
Manually annotated by BRENDA team
Reinton, N.; Haugen, T.B.; Orstavik, S.; Skalhegg, B.S.; Hansson, V.; Jahnsen, T.; Tasken, K.
The gene encoding the C gamma catalytic subunit of cAMP-dependent protein kinase is a transcribed retroposon
Genomics
49
290-297
1998
Homo sapiens, Homo sapiens (P22612)
Manually annotated by BRENDA team
Biondi, R.M.; Nebreda, A.R.
Signalling specificity of Ser/Thr protein kinases through docking-site-mediated interactions
Biochem. J.
372
1-13
2003
Homo sapiens
Manually annotated by BRENDA team
Soulsby, M.D.; Wojcikiewicz, R.J.H.
The type III inositol 1,4,5-trisphosphate receptor is phosphorylated by cAMP-dependent protein kinase at three sites
Biochem. J.
392
493-497
2005
Homo sapiens
Manually annotated by BRENDA team
Kuznetsov, A.; Uri, A.; Raidaru, G.; Jarv, J.
Kinetic analysis of inhibition of cAMP-dependent protein kinase catalytic subunit by the peptide-nucleoside conjugate AdcAhxArg6
Bioorg. Chem.
32
527-535
2004
Homo sapiens
Manually annotated by BRENDA team
Orstavik, S.; Funderud, A.; Hafte, T.T.; Eikvar, S.; Jahnsen, T.; Skalhegg, B.S.
Identification and characterization of novel PKA holoenzymes in human T lymphocytes
FEBS J.
272
1559-1567
2005
Homo sapiens
Manually annotated by BRENDA team
Rapacciuolo, A.; Suvarna, S.; Barki-Harrington, L.; Luttrell, L.M.; Cong, M.; Lefkowitz, R.J.; Rockman, H.A.
Protein kinase A and G protein-coupled receptor kinase phosphorylation mediates beta-1 adrenergic receptor endocytosis through different pathways
J. Biol. Chem.
278
35403-35411
2003
Homo sapiens
Manually annotated by BRENDA team
Piontek, J.; Brandt, R.
Differential and regulated binding of cAMP-dependent protein kinase and protein kinase C isoenzymes to gravin in human model neurons: evidence that gravin provides a dynamic platform for the localization of kinases during neuronal development
J. Biol. Chem.
278
38970-38979
2003
Homo sapiens
Manually annotated by BRENDA team
Sewer, M.B.; Waterman, M.R.
cAMP-dependent protein kinase enhances CYP17 transcription via MKP-1 activation in H295R human adrenocortical cells
J. Biol. Chem.
278
8106-8111
2003
Homo sapiens
Manually annotated by BRENDA team
Alfthan, K.; Heiska, L.; Gronholm, M.; Renkema, G.H.; Carpen, O.
Cyclic AMP-dependent protein kinase phosphorylates merlin at serine 518 independently of p21-activated kinase and promotes merlin-ezrin heterodimerization
J. Biol. Chem.
279
18559-18566
2004
Homo sapiens
Manually annotated by BRENDA team
Gardner, L.A.; Delos Santos, N.M.; Matta, S.G.; Whitt, M.A.; Bahouth, S.W.
Role of the cyclic AMP-dependent protein kinase in homologous resensitization of the beta1-adrenergic receptor
J. Biol. Chem.
279
21135-21143
2004
Homo sapiens
Manually annotated by BRENDA team
Viste, K.; Kopperud, R.K.; Christensen, A.E.; Doskeland, S.O.
Substrate enhances the sensitivity of type I protein kinase a to cAMP
J. Biol. Chem.
280
13279-13284
2005
Bos taurus, Homo sapiens
Manually annotated by BRENDA team
D'Souza, T.; Agarwal, R.; Morin, P.J.
Phosphorylation of claudin-3 at threonine 192 by cAMP-dependent protein kinase regulates tight junction barrier function in ovarian cancer cells
J. Biol. Chem.
280
26233-26240
2005
Homo sapiens
Manually annotated by BRENDA team
Horner, T.J.; Osawa, S.; Schaller, M.D.; Weiss, E.R.
Phosphorylation of GRK1 and GRK7 by cAMP-dependent protein kinase attenuates their enzymatic activities
J. Biol. Chem.
280
28241-28250
2005
Bos taurus, Homo sapiens
Manually annotated by BRENDA team
Zhang, Y.; Li, H.L.; Wang, D.L.; Liu, S.J.; Wang, J.Z.
A transitory activation of protein kinase-A induces a sustained tau hyperphosphorylation at multiple sites in N2a cells-imply a new mechanism in Alzheimer pathology
J. Neural Transm.
2
1-11
2006
Homo sapiens
Manually annotated by BRENDA team
Lee, H.; Rezai-Zadeh, N.; Seto, E.
Negative regulation of histone deacetylase 8 activity by cyclic AMP-dependent protein kinase A
Mol. Cell. Biol.
24
765-773
2004
Homo sapiens
Manually annotated by BRENDA team
Tian, L.; Coghill, L.S.; McClafferty, H.; MacDonald, S.H.; Antoni, F.A.; Ruth, P.; Knaus, H.G.; Shipston, M.J.
Distinct stoichiometry of BKCa channel tetramer phosphorylation specifies channel activation and inhibition by cAMP-dependent protein kinase
Proc. Natl. Acad. Sci. USA
101
11897-11902
2004
Bos taurus, Homo sapiens
Manually annotated by BRENDA team
Howe, A.K.; Baldor, L.C.; Hogan, B.P.
Spatial regulation of the cAMP-dependent protein kinase during chemotactic cell migration
Proc. Natl. Acad. Sci. USA
102
14320-14325
2005
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Zhang, W.; Morris, G.Z.; Beebe, S.J.
Characterization of the cAMP-dependent protein kinase catalytic subunit Cgamma expressed and purified from sf9 cells
Protein Expr. Purif.
35
156-169
2004
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Conran, N.; Almeida, C.B.; Lanaro, C.; Ferreira, R.P.; Traina, F.; Saad, S.T.; Costa, F.F.
Inhibition of caspase-dependent spontaneous apoptosis via a cAMP-protein kinase A dependent pathway in neutrophils from sickle cell disease patients
Br. J. Haematol.
139
148-158
2007
Homo sapiens
Manually annotated by BRENDA team
Humphries, K.M.; Pennypacker, J.K.; Taylor, S.S.
Redox regulation of cAMP-dependent protein kinase signaling: kinase versus phosphatase inactivation
J. Biol. Chem.
282
22072-22079
2007
Homo sapiens
Manually annotated by BRENDA team
Borthwick, L.A.; McGaw, J.; Conner, G.; Taylor, C.J.; Gerke, V.; Mehta, A.; Robson, L.; Muimo, R.
The formation of the cAMP/protein kinase A-dependent annexin 2 S100A10 complex with cystic fibrosis conductance regulator protein (CFTR) regulates CFTR channel function
Mol. Biol. Cell
18
3388-3397
2007
Homo sapiens
Manually annotated by BRENDA team
Cheng, Y.; Zhang, Y.; McCammon, J.A.
How does activation loop phosphorylation modulate catalytic activity in the cAMP-dependent protein kinase: a theoretical study
Protein Sci.
15
672-683
2006
Homo sapiens
Manually annotated by BRENDA team
Shemetov, A.A.; Seit-Nebi, A.S.; Bukach, O.V.; Gusev, N.B.
Phosphorylation by cyclic AMP-dependent protein kinase inhibits chaperone-like activity of human HSP22 in vitro
Biochemistry
73
200-208
2008
Homo sapiens
Manually annotated by BRENDA team
Taylor, S.S.; Kim, C.; Cheng, C.Y.; Brown, S.H.; Wu, J.; Kannan, N.
Signaling through cAMP and cAMP-dependent protein kinase: diverse strategies for drug design
Biochim. Biophys. Acta
1784
16-26
2008
Homo sapiens
Manually annotated by BRENDA team
Kleiveland, C.R.; Kassem, M.; Lea, T.
Human mesenchymal stem cell proliferation is regulated by PGE2 through differential activation of cAMP-dependent protein kinase isoforms
Exp. Cell Res.
314
1831-1838
2008
Homo sapiens
Manually annotated by BRENDA team
Larsen, A.C.; Kvissel, A.K.; Hafte, T.T.; Avellan, C.I.; Eikvar, S.; Rootwelt, T.; Orstavik, S.; Skalhegg, B.S.
Inactive forms of the catalytic subunit of protein kinase A are expressed in the brain of higher primates
FEBS J.
275
250-262
2008
Homo sapiens
Manually annotated by BRENDA team
Islam, A.; Jones, H.; Hiroi, T.; Lam, J.; Zhang, J.; Moss, J.; Vaughan, M.; Levine, S.J.
cAMP-dependent protein kinase A (PKA) signaling induces TNFR1 exosome-like vesicle release via anchoring of PKA regulatory subunit RIIbeta to BIG2
J. Biol. Chem.
283
25364-25371
2008
Homo sapiens
Manually annotated by BRENDA team
Dammer, E.B.; Sewer, M.B.
Phosphorylation of CtBP1 by cAMP-dependent protein kinase modulates induction of CYP17 by stimulating partnering of CtBP1 and 2
J. Biol. Chem.
283
6925-6934
2008
Homo sapiens
Manually annotated by BRENDA team
Chatelier, A.; Dahllund, L.; Eriksson, A.; Krupp, J.; Chahine, M.
Biophysical properties of human Na v1.7 splice variants and their regulation by protein kinase A
J. Neurophysiol.
99
2241-2250
2008
Homo sapiens
Manually annotated by BRENDA team
Farquhar, M.J.; Harris, H.J.; Diskar, M.; Jones, S.; Mee, C.J.; Nielsen, S.U.; Brimacombe, C.L.; Molina, S.; Toms, G.L.; Maurel, P.; Howl, J.; Herberg, F.W.; van Ijzendoorn, S.C.; Balfe, P.; McKeating, J.A.
Protein kinase A-dependent step(s) in hepatitis C virus entry and infectivity
J. Virol.
82
8797-8811
2008
Homo sapiens
Manually annotated by BRENDA team
Das, P.; Ezashi, T.; Gupta, R.; Roberts, R.M.
Combinatorial roles of protein kinase A, Ets2, and 3,5-cyclic-adenosine monophosphate response element-binding protein-binding protein/p300 in the transcriptional control of interferon-tau expression in a trophoblast cell line
Mol. Endocrinol.
22
331-343
2008
Homo sapiens
Manually annotated by BRENDA team
Dai, R.; Ali, M.K.; Lezcano, N.; Bergson, C.
A crucial role for cAMP and protein kinase A in D1 dopamine receptor regulated intracellular calcium transients
Neurosignals
16
112-123
2008
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
Desiniotis, A.; Schaefer, G.; Klocker, H.; Eder, I.E.
Enhanced antiproliferative and proapoptotic effects on prostate cancer cells by simultaneously inhibiting androgen receptor and cAMP-dependent protein kinase A
Int. J. Cancer
126
775-789
2010
Homo sapiens
Manually annotated by BRENDA team
Papa, S.; Scacco, S.; De Rasmo, D.; Signorile, A.; Papa, F.; Panelli, D.; Nicastro, A.; Scaringi, R.; Santeramo, A.; Roca, E.; Trentadue, R.; Larizza, M.
cAMP-dependent protein kinase regulates post-translational processing and expression of complex I subunits in mammalian cells
Biochim. Biophys. Acta
1797
649-658
2010
Homo sapiens, Mus musculus, Mus musculus BALB/c, Rattus norvegicus
Manually annotated by BRENDA team
Gerlits, O.; Waltman, M.J.; Taylor, S.; Langan, P.; Kovalevsky, A.
Insights into the phosphoryl transfer catalyzed by cAMP-dependent protein kinase: an X-ray crystallographic study of complexes with various metals and peptide substrate SP20
Biochemistry
52
3721-3727
2013
Homo sapiens (P61925)
Manually annotated by BRENDA team
Huang, S.; Li, Q.; Alberts, I.; Li, X.
PRKX, a novel cAMP-dependent protein kinase member, plays an important role in development
J. Cell. Biochem.
117
566-573
2016
Homo sapiens, Homo sapiens (P17612)
Manually annotated by BRENDA team
Komal, P.; Estakhr, J.; Kamran, M.; Renda, A.; Nashmi, R.
cAMP-dependent protein kinase inhibits alpha7 nicotinic receptor activity in layer 1 cortical interneurons through activation of D1/D5 dopamine receptors
J. Physiol.
593
3513-3532
2015
Homo sapiens
Manually annotated by BRENDA team
Liu, Q.; Nguyen, E.; Doskeland, S.; Segal-Bendirdjian, E.
cAMP-dependent protein kinase A (PKA)-mediated c-Myc degradation is dependent on the relative proportion of PKA-I and PKA-II isozymes
Mol. Pharmacol.
88
469-476
2015
Homo sapiens
Manually annotated by BRENDA team
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