Information on EC 2.7.11.11 - cAMP-dependent protein kinase and Organism(s) Homo sapiens and UniProt Accession P22612

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IUBMB Comments
cAMP is required to activate this enzyme. The inactive holoenzyme of cAMP-dependent protein kinase is a tetramer composed of two regulatory (R) and two catalytic (C) subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP molecules and two free monomeric catalytic subunits [i.e. R2C2 + 4 cAMP = R2(cAMP)4 + 2 C].
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Select one or more organisms in this record: ?
This record set is specific for:
Homo sapiens
UNIPROT: P22612
Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Archaea, Bacteria
Reaction Schemes
Synonyms
3',5'-cyclic adenosine monophosphate-dependent protein kinase, cAMP dependent protein kinase, cAMP-dependent PKA, cAMP-dependent protein kinase, cAMP-dependent protein kinase A, cAMP-dependent protein kinase catalytic subunit, cAMP-dependent protein kinase catalytic subunit (C-subunit), cAMP-dependent protein kinase type 1, cAMP-dependent protein kinase type 2, cAMP-dependent protein kinase type 3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3',5'-cyclic adenosine monophosphate-dependent protein kinase
265790, 265795
-
cAMP-dependent protein kinase
cAMP-dependent protein kinase A
247
-
cAMP-dependent protein kinase, alpha-catalytic subunit
265790
-
cAMP-dependent protein kinase, beta-catalytic subunit
265795
-
cAMP-dependent protein kinase, gamma-catalytic subunit
265794
-
cAMP/protein kinase A
247
-
cyclic AMP-dependent protein kinase
247
-
cyclic AMP-dependent protein kinase A
247
-
PK-25
-
-
-
-
PKA C-alpha
-
-
-
-
PKA C-beta
-
-
-
-
PKA C-gamma
-
-
-
-
PKA RIalpha
247
regulatory subunit I alpha of PKA
PKA type I
247
isoform
PKA type II
PKA-Calpha
265790
-
PKA-I
247
isozyme
PKA-II
247
isozyme
PKAc
306132
catalytic subunit
PKACalpha
265790
-
PKACbeta
265795
-
PRKACA
265790
catalytic subunit
protein kinase A
protein kinase A type II
247
-
protein kinase-A
247
-
type I protein kinase A
247
-
type II protein kinase A
247
-
additional information
247
see also EC 2.7.11.26
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + a protein = ADP + a phosphoprotein
show the reaction diagram
catalytic mechanism
-
SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (cAMP-dependent)
cAMP is required to activate this enzyme. The inactive holoenzyme of cAMP-dependent protein kinase is a tetramer composed of two regulatory (R) and two catalytic (C) subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP molecules and two free monomeric catalytic subunits [i.e. R2C2 + 4 cAMP = R2(cAMP)4 + 2 C].
CAS REGISTRY NUMBER
COMMENTARY hide
142008-29-5
-
142008-29-5
cAMP-dependent protein kinase
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + AID protein
ADP + phosphorylated AID protein
show the reaction diagram
-
-
-
?
ATP + alpha7 nicotinic acetylcholine receptor
ADP + phosphorylated alpha7 nicotinic acetylcholine receptor
show the reaction diagram
-
phosphorylation at Ser365
-
-
?
ATP + bAD protein
ADP + phosphorylated bAD protein
show the reaction diagram
-
-
-
?
ATP + beta-adrenergic receptor
ADP + phosphorylated beta-adrenergic receptor
show the reaction diagram
ATP + beta1-adrenergic receptor
ADP + phosphorylated beta1-adrenergic receptor
show the reaction diagram
ATP + BKCa channel ZERO
ADP + phosphorylated BKCa channel ZERO
show the reaction diagram
ATP + c-Myc protein
ADP + phosphorylated c-Myc protein
show the reaction diagram
-
-
-
-
?
ATP + calpain 2
ADP + phosphorylated calpain 2
show the reaction diagram
-
-
-
?
ATP + carboxyl-terminal binding protein 1
ADP + phosphorylated carboxyl-terminal binding protein 1
show the reaction diagram
-
-
-
-
?
ATP + CFTR protein
ADP + phosphorylated CFTR protein
show the reaction diagram
-
-
-
?
ATP + claudin-3
ADP + phosphorylated claudin-3
show the reaction diagram
ATP + claudin-3 S199A
ADP + phosphorylated claudin-3 S199A
show the reaction diagram
-
recombinant GST-fusion claudin-3 mutant S199A expressed in Escherichia coli, phosphorylation at Thr192, activity of catalytic PKA subunit is lower compared to wild-type claudin-3
-
-
?
ATP + CREb protein
ADP + phosphorylated CREb protein
show the reaction diagram
-
-
-
?
ATP + G protein-coupled receptor GRK1
ADP + phosphorylated G protein-coupled receptor GRK1
show the reaction diagram
ATP + G protein-coupled receptor GRK7
ADP + phosphorylated G protein-coupled receptor GRK7
show the reaction diagram
ATP + glycogen synthase kinase 3beta
ADP + phosphorylated glycogen synthase kinase 3beta
show the reaction diagram
-
phosphorylation of glycogen synthase kinase 3beta is carried out by the PKA type II isoform
-
-
?
ATP + GRK2 protein
ADP + phosphorylated GRK2 protein
show the reaction diagram
-
-
-
?
ATP + GSK3 protein
ADP + phosphorylated GSK3 protein
show the reaction diagram
-
-
-
?
ATP + histone
ADP + phosphorylated histone
show the reaction diagram
-
-
-
-
?
ATP + histone 3s
ADP + phosphorylated histone 3s
show the reaction diagram
-
activity of the catalytic subunit Cgamma
-
-
?
ATP + histone deacetylase 8
ADP + phosphorylated histone deacetylase 8
show the reaction diagram
ATP + HSL protein
ADP + phosphorylated HSL protein
show the reaction diagram
-
-
-
?
ATP + KCNN2 protein
ADP + phosphorylated KCNN2 protein
show the reaction diagram
-
-
-
?
ATP + Kemptide
ADP + Kemptide phosphate
show the reaction diagram
ATP + kemptide
ADP + phospho-kemptide
show the reaction diagram
-
-
-
-
?
ATP + Kemptide
ADP + phosphorylated Kemptide
show the reaction diagram
ATP + LRRASLG
ADP + LRRA-phosphoserine-LG
show the reaction diagram
-
i.e. Kemptide, recombinant PKA catalytic subunit
-
-
?
ATP + merlin
ADP + phosphorylated merlin
show the reaction diagram
ATP + mitogen-activated protein kinase phosphatase-1
ADP + phosphorylated mitogen-activated protein kinase phosphatase-1
show the reaction diagram
ATP + NDUFS4 subunit of complex I
ADP + phosphorylated NDUFS4 subunit of complex I
show the reaction diagram
-
complex I is the NADH-ubiquinone oxidoreductase, E.C 1.6.5.3
-
-
?
ATP + NFAT2 protein
ADP + phosphorylated NFAT2 protein
show the reaction diagram
-
-
-
?
ATP + PKA regulatory subunit IIalpha
ADP + phosphorylated PKA regulatory subunit IIalpha
show the reaction diagram
-
-
-
-
?
ATP + protein tyrosine phosphatase
ADP + phosphorylated protein tyrosine phosphatase
show the reaction diagram
-
i.e. PTP, contains a PEST motif
-
-
?
ATP + Rap1a
ADP + phosphorylated Rap1a
show the reaction diagram
ATP + Rap1b
ADP + phosphorylated Rap1b
show the reaction diagram
ATP + RFARKGSLREKNV
ADP + RFARKG-phosphoserine-LREKNV
show the reaction diagram
-
protein kinase C-derived peptide, activity of the catalytic subunit Cgamma
-
-
?
ATP + RKRSRAE
ADP + RKR-phosphoserine-RAE
show the reaction diagram
-
cGPK-1-derived peptide, activity of the catalytic subunit Cgamma
-
-
?
ATP + RKRSRKE
ADP + RKR-phosphoserine-RKE
show the reaction diagram
-
cGPK-2-derived peptide, activity of the catalytic subunit Cgamma
-
-
?
ATP + RRLSSLRA
ADP + RRL-phosphoserine-phosphoserine-LRA
show the reaction diagram
-
S6 kinase-derived peptide, activity of the catalytic subunit Cgamma
-
-
?
ATP + small heat shock protein HSP22
ADP + phosphorylated small heat shock protein HSP22
show the reaction diagram
-
-
-
-
?
ATP + SP20 protein
ADP + phosphorylated SP20 protein
show the reaction diagram
i.e. TTYADFIASGRTGRRASIHD
-
-
?
ATP + type III inositol 1,4,5-trisphosphate receptor
ADP + phosphorylated type III inositol 1,4,5-trisphosphate receptor
show the reaction diagram
ATP + tyrosine hydroxylase
ADP + phosphorylated tyrosine hydroxylase
show the reaction diagram
-
activity of catalytic PKA subunit
-
-
?
ATP + VASP
ADP + phosphorylated VASP
show the reaction diagram
-
i.e. vasodilator-stimulated phosphoprotein
-
-
?
ATP + VASP protein
ADP + phosphorylated VASP protein
show the reaction diagram
-
-
-
?
ATP + [tau-protein]
ADP + O-phospho-[tau-protein]
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + AID protein
ADP + phosphorylated AID protein
show the reaction diagram
-
-
-
?
ATP + alpha7 nicotinic acetylcholine receptor
ADP + phosphorylated alpha7 nicotinic acetylcholine receptor
show the reaction diagram
-
phosphorylation at Ser365
-
-
?
ATP + bAD protein
ADP + phosphorylated bAD protein
show the reaction diagram
-
-
-
?
ATP + beta-adrenergic receptor
ADP + phosphorylated beta-adrenergic receptor
show the reaction diagram
-
PKA phosphorylation mediates beta-1 adrenergic receptor endocytosis via the caveolae pathway
-
-
?
ATP + beta1-adrenergic receptor
ADP + phosphorylated beta1-adrenergic receptor
show the reaction diagram
-
recombinant beta1-adrenergic receptor expressed in HEK-293 cell membranes, phosphorylation at Ser312 is essential for activation of endocytic recycling of the agonist-internalized beta1-adrenergic receptor, beta1-AR mutant S312A is not recycled, overview
-
-
?
ATP + BKCa channel ZERO
ADP + phosphorylated BKCa channel ZERO
show the reaction diagram
-
recombinant murine HA-tagged tetrameric protein expressed in HEK-293 cells, activity with wild-type and mutant Y334V channel tetramers, but no activity with S899A mutant, phosphorylation at Ser899 activates the channels, overview
-
-
?
ATP + c-Myc protein
ADP + phosphorylated c-Myc protein
show the reaction diagram
-
-
-
-
?
ATP + calpain 2
ADP + phosphorylated calpain 2
show the reaction diagram
-
-
-
?
ATP + CFTR protein
ADP + phosphorylated CFTR protein
show the reaction diagram
-
-
-
?
ATP + claudin-3
ADP + phosphorylated claudin-3
show the reaction diagram
-
high activity in ovarian cancer cells with recombinantly overexpressed claudin-3, phosphorylation of claudin-3 affecting the barrier function with extracellular Ca2+, overview
-
-
?
ATP + CREb protein
ADP + phosphorylated CREb protein
show the reaction diagram
-
-
-
?
ATP + G protein-coupled receptor GRK1
ADP + phosphorylated G protein-coupled receptor GRK1
show the reaction diagram
-
recombinant FLAG-tagged GRK1 expressed in HEK-293 cells, enzymatic inactivation of the receptor activity inhibiting rhodopsin phosphorylation
-
-
?
ATP + G protein-coupled receptor GRK7
ADP + phosphorylated G protein-coupled receptor GRK7
show the reaction diagram
-
recombinant FLAG-tagged GRK1 expressed in HEK-293 cells, enzymatic inactivation of the receptor activity inhibiting rhodopsin phosphorylation
-
-
?
ATP + GRK2 protein
ADP + phosphorylated GRK2 protein
show the reaction diagram
-
-
-
?
ATP + GSK3 protein
ADP + phosphorylated GSK3 protein
show the reaction diagram
-
-
-
?
ATP + histone deacetylase 8
ADP + phosphorylated histone deacetylase 8
show the reaction diagram
-
FLAG-tagged class 1 histone deacetylase HDAC8 expressed in HeLa cells via adenovirus infection, phosphorylation at Ser39 reduces the enzyme activity of HDAC8, hyperphosphorylation inhibits the enzyme
-
-
?
ATP + HSL protein
ADP + phosphorylated HSL protein
show the reaction diagram
-
-
-
?
ATP + KCNN2 protein
ADP + phosphorylated KCNN2 protein
show the reaction diagram
-
-
-
?
ATP + Kemptide
ADP + phosphorylated Kemptide
show the reaction diagram
-
-
-
?
ATP + merlin
ADP + phosphorylated merlin
show the reaction diagram
-
recombinant human substrate protein expressed in HEK-293 cells, activity of the catalytic subunit C, phosphorylation at Ser518 induces N-terminal binding of merlin to ezrin
-
-
?
ATP + mitogen-activated protein kinase phosphatase-1
ADP + phosphorylated mitogen-activated protein kinase phosphatase-1
show the reaction diagram
-
the enzyme enhances steroid hydroxylase CYP17 transcription via mitogen-activated protein kinase phosphatase-1 MKP-1 activation in H295R adrenocortical cells
-
-
?
ATP + NDUFS4 subunit of complex I
ADP + phosphorylated NDUFS4 subunit of complex I
show the reaction diagram
-
complex I is the NADH-ubiquinone oxidoreductase, E.C 1.6.5.3
-
-
?
ATP + NFAT2 protein
ADP + phosphorylated NFAT2 protein
show the reaction diagram
-
-
-
?
ATP + Rap1a
ADP + phosphorylated Rap1a
show the reaction diagram
phosphorylation of Rap1a on serine 180. Phosphorylation creates potential binding sites for the adaptor protein 14-3-3 that links Rap1 to the scaffold protein KSR. Because KSR can exist as a dimer with B-Raf, this provides Rap1 an indirect link to B-Raf and the MAP kinase cascade
-
-
?
ATP + Rap1b
ADP + phosphorylated Rap1b
show the reaction diagram
phosphorylation of Rap1b on serine 179. Phosphorylation creates potential binding sites for the adaptor protein 14-3-3 that links Rap1 to the scaffold protein KSR
-
-
?
ATP + SP20 protein
ADP + phosphorylated SP20 protein
show the reaction diagram
i.e. TTYADFIASGRTGRRASIHD
-
-
?
ATP + type III inositol 1,4,5-trisphosphate receptor
ADP + phosphorylated type III inositol 1,4,5-trisphosphate receptor
show the reaction diagram
-
i.e. IP3 receptor, recombinantly expressed in HEK cells, forms tetrameric Ca2+ channels in the endoplasmic reticulum
-
-
?
ATP + VASP protein
ADP + phosphorylated VASP protein
show the reaction diagram
-
-
-
?
ATP + [tau-protein]
ADP + O-phospho-[tau-protein]
show the reaction diagram
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cAMP
dependent on
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
-
-
additional information
-
in HeLa cells PKA activity follows a biphasic response to thiol oxidation, overview
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
AdcAhxArg6
-
i.e. adenosine 5'-carboxylic acid-6-aminohexanoic acid-L-arginine, peptide-nucleoside conjugate inhibitor, inhibition of the catalytic subunit, binding mechanism
adenosine 3',5'-cyclic phosphorothioate
-
-
beta,gamma-methyleneadenosine 5'-triphosphate
-
cAMP-dependent protein kinase inhibitor(5-24)
peptide substrate-competitive inhibitor
-
KT-5720
-
-
KT5720
-
-
myristoylated PKI
-
specific inhibitor
-
myristoylated protein kinase I (14-22) amide
-
specific PKA inhibitor
-
N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide
i.e. H89, ATP-competitive inhibitor
-
N2-(6-([N2-(9-(4-[7-(2-ethoxy-2-oxoethyl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]piperazin-1-yl)-9-oxononanoyl)-D-arginyl]amino)hexanoyl)-D-argininamide
i.e. ARC-2116, very weak bisubstrate inhibitor of PKAcalpha
-
N2-(6-[(N-(9-oxo-9-[4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)piperazin-1-yl]nonanoyl)-D-alanyl)amino]hexanoyl)-D-arginyl-D-arginyl-D-arginyl-D-arginyl-D-arginyl-D-argininamide
i.e. ARC-2123, photocaging of the tight-binding bisubstrate inhibitor of cAMP-dependent protein kinase (PKA) with a nitrodibenzofuran-based group fully abolishes its inhibitory potency
-
N2-(6-[(N2-(9-oxo-9-[4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)piperazin-1-yl]nonanoyl)-D-arginyl)amino]hexanoyl)-D-argininamide
i.e. ARC-1408 , photocaging of the tight-binding bisubstrate inhibitor of cAMP-dependent protein kinase (PKA) with a nitrodibenzofuran-based group fully abolishes its inhibitory potency
-
N2-(6-[(N6-[1-(4,4-dimethyl-2,6-dioxocyclohexylidene)-3-methylbutyl]-N2-(9-oxo-9-[4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)piperazin-1-yl]nonanoyl)-D-lysyl)amino]hexanoyl)-D-argininamide
i.e. ARC-2115, photocaging of the tight-binding bisubstrate inhibitor of cAMP-dependent protein kinase (PKA) with a nitrodibenzofuran-based group fully abolishes its inhibitory potency
-
N2-(9-oxo-9-[4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)piperazin-1-yl]nonanoyl)-D-arginyl-D-arginyl-D-arginyl-D-arginyl-D-arginyl-D-arginyl-D-lysinamide
i.e. ARC-1411, photocaging of the tight-binding bisubstrate inhibitor of cAMP-dependent protein kinase (PKA) with a nitrodibenzofuran-based group fully abolishes its inhibitory potency
-
N2-[6-((N-[9-(4-(7-[(3-nitrodibenzo[b,d]furan-2-yl)methyl]-7H-pyrrolo[2,3-d]pyrimidin-4-yl)piperazin-1-yl)-9-oxononanoyl]-D-alanyl)amino)hexanoyl]-D-arginyl-D-arginyl-D-arginyl-D-arginyl-D-arginyl-D-argininamide
i.e. ARC-2113, very weak bisubstrate inhibitor of PKAcalpha
-
N6-[1-(4,4-dimethyl-2,6-dioxocyclohexylidene)-3-methylbutyl]-N2-(6-[(N2-(9-oxo-9-[4-(7H-pyrrolo[2,3-d]pyrimidin-4-yl)piperazin-1-yl]nonanoyl)-D-arginyl)amino]hexanoyl)-D-lysinamide
i.e. ARC-2114, photocaging of the tight-binding bisubstrate inhibitor of cAMP-dependent protein kinase (PKA) with a nitrodibenzofuran-based group fully abolishes its inhibitory potency
-
PKA regulatory subunit Ialpha
-
pseudosubstrate inhibitor
-
PKI
-
protein kinase inhibitor peptide
-
PKI
-
pseudosubstrate peptides
-
IC50 values for inhibition of the regulatory subunits of PKA by endogenous inhibitors, overview
-
RIIalpha subunit
-
-
-
STVHEILCKLSLEG
-
an acetylated peptide Ac1-14, but not the nonacetylated equivalent N1-14, inhibits PKA-dependent outwardly rectifying Cl- channels, ORCC, and CFTRmediated currents, the peptide sequence is equivalent to the S100A10 binding site on anx 2, it disrupts the anx S100A10/CTFR interaction
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8-bromo-cAMP
8-CPT-cAMP
-
induces release of the active catalytic subunits from the PKA holoenzyme
Adrenocorticotropin
-
induces PKA activation
-
Calcineurin
-
i.e. CaN, is important for the cAMP/PKA-dependent anx 2–S100A10 complex formation
-
cAMP-response element-binding protein-binding protein/p300
-
PKA coactivator
-
dibutyryl-cAMP
-
induces MKP-1 mRNA and protein expression in H295R cells
forskolin
isoproterenol
-
stimulates
N6,2'-O-dibutyryladenosine 3',5'-cyclic phosphate
-
induces PKA activation
prostaglandin E1
-
activates
prostaglandin E2
-
0.05 mM induce activity by elevation of cAMP levels
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0169 - 0.0276
ATP
0.00974 - 0.0609
Kemptide
0.0253
RFARKGSLREKNV
-
pH 7.0, 30°C, recombinant catalytic subunit Cgamma
0.0333
RKRSRAE
-
pH 7.0, 30°C, recombinant catalytic subunit Cgamma
0.0333
RKRSRKE
-
pH 7.0, 30°C, recombinant catalytic subunit Cgamma
0.0503
RRLSSLRA
-
pH 7.0, 30°C, recombinant catalytic subunit Cgamma
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.15 - 6.03
ATP
7.5 - 14.5
Kemptide
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
77.9 - 356.8
ATP
123.2 - 1488.7
Kemptide
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00013
AdcAhxArg6
-
pH 7.5, 30°C, recombinant catalytic subunit
0.0000037 - 0.00125
cAMP-dependent protein kinase inhibitor(5-24)
-
0.0000255 - 0.000278
N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide
-
additional information
additional information
-
inhibition kinetics for the catalytic subunit
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000074 - 0.00187
cAMP-dependent protein kinase inhibitor(5-24)
-
0.0000606 - 0.0000633
N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.31
-
purified recombinant catalytic subunit Cgamma, substrate Kemptide
0.81
-
purified recombinant catalytic subunit Cgamma, substrate histone
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
-
assay at
7
-
assay at
7.2
-
assay at
7.5
-
assay at
7.6
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
of patients with adrenocorticotropic hormone-independent Cushing's syndrome
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
mesenchymal cell
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
HEK-293 cells that stably express D1 receptors
Manually annotated by BRENDA team
-
nasal
Manually annotated by BRENDA team
-
adut skin and NHDF neonatal human dermal fibroblasts
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
immortilized ovarian surface epithelial cell line
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
from sickle cell disease patients
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
ovarian cancer cell line
Manually annotated by BRENDA team
-
fetal lung fibroblast-like cell line
Manually annotated by BRENDA team
additional information
-
high PKA activity in ovarian cancer cells
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
cAMP/PKA-dependent anx 2–S100A10 complex
Manually annotated by BRENDA team
additional information
-
the enzyme binds to and colocalizes with the kinase-anchoring protein gravin in the neuronal cortex membrane which is enriched in the inner peripheral cortex in close proximity to the plasma membrane
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
exploration and elucidation of the evolution of the alternative 5' exons and the splicing pattern giving rise to the numerous PKA catalytic subunit isoforms. Alignment of the segments encoded by Calpha1- and Cbeta1-specific 5'exons
malfunction
-
combined inhibition of the androgen receptor and the regulatory subunit I alpha of PKA with small interference RNAs significantly increases the growth-inhibitory and proapoptotic effects of androgen receptor knockdown. Downregulating PKA RIalpha is sufficient to inhibit PKA signaling and also impairs androgen receptor expression and activation. Depletion of PKA RIalpha also potentiates the antiproliferative effect of the antiandrogen bicalutamide in androgen-sensitive LNCaP cells
physiological function
additional information
-
NDUFS4 mutations result in defective assembly of complex I with severe effects on the respiratory chain, mouse model NDUFS4 point mutations and phenotypes, overview
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
KAPCG_HUMAN
351
0
40434
Swiss-Prot
other Location (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41000
-
1 * 41000, isolated recombinant catalytic subunit Cgamma from Sf9 cells, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
oligomer
-
-
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
-
activation loop phosphorylation at Thr197 regulates the enzyme's catalytic activity, molecular mechanism, classical molecular dynamics simulations and ab initio QM/MM calculations are carried out on the wild-type PKA-Mg2+- ATP-substrate complex and its dephosphorylated mutant, T197A, overview
additional information
unmyristylated Calpha2 may be essential for fertility in the male
CRYSTALLIZATION (Commentary)