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Information on EC 2.7.11.1 - non-specific serine/threonine protein kinase and Organism(s) Archaeoglobus fulgidus and UniProt Accession O28471

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IUBMB Comments
This is a heterogeneous group of serine/threonine protein kinases that do not have an activating compound and are either non-specific or their specificity has not been analysed to date.
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This record set is specific for:
Archaeoglobus fulgidus
UNIPROT: O28471
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Word Map
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The taxonomic range for the selected organisms is: Archaeoglobus fulgidus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
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ATP
+
a [protein]-(L-serine/L-threonine)
=
ADP
+
a [protein]-(L-serine/L-threonine) phosphate
+
a [protein]-(L-serine/L-threonine)
=
+
a [protein]-(L-serine/L-threonine) phosphate
Synonyms
protein kinase a, 14-3-3, camkii, p-akt, lrrk2, c-jun n-terminal kinase, serine/threonine kinase, raf-1, protein kinase b, gsk-3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
serine kinase Rio1
-
A-kinase
-
-
-
-
A-T, mutated
-
-
-
-
A-T, mutated homolog
-
-
-
-
AMH type II receptor
-
-
-
-
Anti-sigma B factor rsbT
-
-
-
-
AP50 kinase
-
-
-
-
Ataxia telangiectasia mutated
-
-
-
-
Ataxia telangiectasia mutated homolog
-
-
-
-
ATP-protein transphosphorylase
-
-
-
-
betaIIPKC
-
-
-
-
Breast-tumor-amplified kinase
-
-
-
-
Calcium-dependent protein kinase C
-
-
-
-
Calcium/phospholipid-dependent protein kinase
-
-
-
-
cAMP-dependent protein kinase A
-
-
-
-
casein kinase
-
-
-
-
casein kinase 2
-
-
-
-
casein kinase I
-
-
-
-
casein kinase II
-
-
-
-
casein kinase-2
-
-
-
-
CDC25 suppressing protein kinase
-
-
-
-
Cell division cycle 2-like
-
-
-
-
CK-2
-
-
-
-
CK2
-
-
-
-
CK2-alpha
-
-
-
-
CKI
-
-
-
-
CKII
-
-
-
-
CLP-36 interacting kinase
-
-
-
-
cyclic monophosphate-dependent protein kinase
-
-
-
-
cyclic nucleotide-dependent protein kinase
-
-
-
-
cyclin-dependent kinase
-
-
-
-
cytidine 3',5'-cyclic monophosphate-responsive protein kinase
-
-
-
-
DNA-PKcs
-
-
-
-
DNPK1
-
-
-
-
dSTPK61
-
-
-
-
G11 protein
-
-
-
-
hARK1
-
-
-
-
histone kinase
-
-
-
-
hPDK1
-
-
-
-
Hpr kinase
-
-
-
-
hPSK
-
-
-
-
HSPK 21
-
-
-
-
HSPK 36
-
-
-
-
hydroxyalkyl-protein kinase
-
-
-
-
Kinase interacting with stathmin
-
-
-
-
kinase, casein (phosphorylating)
-
-
-
-
kinase, protamine (phosphorylating)
-
-
-
-
kinase, protein (phosphorylating)
-
-
-
-
kinase, protein, A (phosphorylating)
-
-
-
-
kinase, protein, C (phosphorylating)
-
-
-
-
Lymphocyte-oriented kinase
-
-
-
-
M phase-specific cdc2 kinase
-
-
-
-
mitogen-activated S6 kinase
-
-
-
-
mPDK1
-
-
-
-
MPSK
-
-
-
-
Myristoylated and palmitoylated serine-threonine kinase
-
-
-
-
NY-REN-55 antigen
-
-
-
-
P-CIP2
-
-
-
-
P460
-
-
-
-
p46Eg265
-
-
-
-
p46XlEg22
-
-
-
-
p82 kinase
-
-
-
-
pEg2
-
-
-
-
phosphorylase b kinase kinase
-
-
-
-
Phototropin
-
-
-
-
Pim-2h
-
-
-
-
PKA
-
-
-
-
PkB kinase
-
-
-
-
pp39-mos
-
-
-
-
Pre-mRNA protein kinase
-
-
-
-
protamine kinase
-
-
-
-
protein glutamyl kinase
-
-
-
-
protein kinase (phosphorylating)
-
-
-
-
Protein kinase B kinase
-
-
-
-
protein kinase CK2
-
-
-
-
Protein kinase Krct
-
-
-
-
Protein kinase MST
-
-
-
-
protein kinase p58
-
-
-
-
Protein kinase PKL12
-
-
-
-
protein phosphokinase
-
-
-
-
protein serine kinase
-
-
-
-
protein serine-threonine kinase
-
-
-
-
protein-aspartyl kinase
-
-
-
-
protein-cysteine kinase
-
-
-
-
protein-serine kinase
-
-
-
-
protein-serine/threonine kinase
-
-
-
-
PRP4 kinase
-
-
-
-
PRP4 pre-mRNA processing factor 4 homolog
-
-
-
-
PSK-H1
-
-
-
-
Raf kinase
-
-
-
-
Raf-1
-
-
-
-
ratAurA
-
-
-
-
ribosomal protein S6 kinase II
-
-
-
-
ribosomal S6 protein kinase
-
-
-
-
RIO serine protein kinase
-
RP1 protein
-
-
-
-
S-receptor kinase
-
-
-
-
serine kinase
-
-
-
-
serine protein kinase
-
-
-
-
serine(threonine) protein kinase
-
-
-
-
serine-specific protein kinase
-
-
-
-
serine/threonine kinase
-
-
-
-
Serine/threonine kinase 15
-
-
-
-
Serine/threonine kinase Ayk1
-
-
-
-
serine/threonine protein kinase
-
-
-
-
Serine/threonine-protein kinase NRK2
-
-
-
-
Serine/threonine-protein kinase NYD-SPK
-
-
-
-
SRK
-
-
-
-
Ste20-like kinase
-
-
-
-
STE20-like kinase MST1
-
-
-
-
STE20-like kinase MST2
-
-
-
-
STE20-like kinase MST3
-
-
-
-
Switch protein/serine kinase
-
-
-
-
T-antigen kinase
-
-
-
-
threonine-specific protein kinase
-
-
-
-
Twitchin kinase
-
-
-
-
type-2 casein kinase
-
-
-
-
additional information
the enzyme belongs to the Rio family, Rio2 subfamily, of serine/threonine protein kinases
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:protein phosphotransferase (non-specific)
This is a heterogeneous group of serine/threonine protein kinases that do not have an activating compound and are either non-specific or their specificity has not been analysed to date.
CAS REGISTRY NUMBER
COMMENTARY hide
191808-15-8
phosphoinositide dependent protein kinase 1
340830-03-7
-
37278-10-7
-
377752-08-4
ribosomal protein S6 kinase 2
389133-24-8
ribosomal S6 kinase 3
52660-18-1
casein kinase, protein kinase CK2
9026-43-1
this CAS Reg. No. encompasses a great variety of protein kinases including the serine/threonine specific kinases
90698-26-3
ribosomal protein S6 kinase 1
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
-
-
?
ATP + alpha-casein
ADP + phosphorylated alpha-casein
show the reaction diagram
dephosphorylated alpha-casein
-
-
?
ATP + histone
ADP + phosphorylated histone
show the reaction diagram
-
-
-
?
ATP + myelin basic protein
ADP + phosphorylated myelin basic protein
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
kinase Rio2 is required for rRNA cleavage in 40S ribosomal subunit maturation
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
binding structure involving residues K168, S53, and D166, overview
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
substrate inhibition. At ATP concentrations above 0.1 mM, kinase activity begins to drop until at 100 mM it becomes almost unmeasurable
Toyocamycin
toyocamycin binds in the ATP binding pocket of the protein more tightly than ATP/Mg2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0008
ATP
pH 8.0, 37°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0071
ATP
pH 8.0, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the enzyme is necessary for the final processing step of maturing the 18S ribosomal rRNA
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30100
x * 30100, calculated from sequence, autophosphorylation by Rio1 reduces oligomer formation and promotes monomerization, resulting in the most active species. Interaction of Rio1 kinase with toyocamycin reveals a conformational switch that controls oligomeric state and catalytic activity
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oligomer
x * 30100, calculated from sequence, autophosphorylation by Rio1 reduces oligomer formation and promotes monomerization, resulting in the most active species. Interaction of Rio1 kinase with toyocamycin reveals a conformational switch that controls oligomeric state and catalytic activity
additional information
the kinase domain is tightly connected to a wHTH domain
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
Ser108 represents the sole autophosphorylation site of Aeroglobus fulgidus Rio1
phosphoprotein
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, high resolution crystal structures of Archaeoglobus fulgidus Rio1 in the presence and absence of bound nucleotides
X-ray crystal structure of toyocamycin bound to Rio1 at 2.0 A, toyocamycin binds in the ATP binding pocket of the protein
hanging drop vapor diffusion in 1 ml wells containing 5–12% poly(ethylene glycol) 900 and 100 mM sodium phosphate/citrate buffer, pH 3.6–4.1. Crystallization of the enzyme after incubation with ATP or ADP and Mn2+. Co-crystal structures of Rio2–ATP–Mn and Rio2–ADP–Mn are solved at 1.84 and 1.75 A resolution, respectively
purified recombinant wild-type and selenomethionine-labeled Rio2, hanging drop vapour diffusion method, equal volumes of protein solution, containing 5-12% PEG 900, and 0.1 M phosphate citrate buffer, pH 3.6-4.1, and reservoir solution, versus 1 ml reservoir solution, purified recombinant Rio2 complexed with ATP using 0.1 M Tris, pH 7.5, 20 mM ATP, 20 mM MgCl2, 20 mM adenosine 5'-(beta,gamma-imino)triphosphate, and 20% ethylene glycol, 20°C, 2-4 days, X-ray diffraction structure determination and analysis at 2.0 A resolution
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
76
Tm-value of unbound enzyme
88
Tm-value in presence of toyocamycin
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant Rio2 from Escherichia coli by adsorption chromatography and gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression of Rio2 in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
LaRonde-LeBlanc, N.; Wlodawer, A.
Crystal structure of A. fulgidus Rio2 defines a new family of serine protein kinases
Structure
12
1585-1594
2004
Archaeoglobus fulgidus (O30245)
Manually annotated by BRENDA team
LaRonde-LeBlanc, N.; Guszczynski, T.; Copeland, T.; Wlodawer, A.
Autophosphorylation of Archaeoglobus fulgidus Rio2 and crystal structures of its nucleotide-metal ion complexes
FEBS J.
272
2800-2810
2005
Archaeoglobus fulgidus (O30245), Archaeoglobus fulgidus
Manually annotated by BRENDA team
Laronde-Leblanc, N.; Guszczynski, T.; Copeland, T.; Wlodawer, A.
Structure and activity of the atypical serine kinase Rio1
FEBS J.
272
3698-3713
2005
Archaeoglobus fulgidus (O28471), Archaeoglobus fulgidus
Manually annotated by BRENDA team
Kiburu, I.N.; LaRonde-LeBlanc, N.
Interaction of Rio1 kinase with toyocamycin reveals a conformational switch that controls oligomeric state and catalytic activity
PLoS ONE
7
e37371
2012
Archaeoglobus fulgidus (O28471), Archaeoglobus fulgidus
Manually annotated by BRENDA team