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Information on EC 2.7.1.B20 - broad specificity nucleoside kinase

for references in articles please use BRENDA:EC2.7.1.B20
preliminary BRENDA-supplied EC number
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UNIPROT: Q2SZE4 not found.
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The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
nucleoside kinase, ta0880, mj0406, pfk-b sugar kinase, bth_i1158, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
NTP:nucleoside 5'-phosphotransferase
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 2'-deoxyadenosine
ADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
-
-
?
ATP + adenosine
ADP + adenosine 5'-phosphate
show the reaction diagram
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
-
-
?
ATP + guanosine
ADP + guanosine 5'-phosphate
show the reaction diagram
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
-
-
?
ATP + inosine
ADP + inosine 5'-phosphate
show the reaction diagram
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
-
-
?
ATP + mizoribine
ADP + mizoribine 5'-phosphate
show the reaction diagram
i.e. 5-hydroxy-1-beta-D-ribofuranosyl-1H-imidazole-4-carboxamide. Phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
-
-
?
ATP + ribavirin
ADP + ribavirin 5'-phosphate
show the reaction diagram
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
-
-
?
ATP + uridine
ADP + uridine 5'-phosphate
show the reaction diagram
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
-
-
?
CTP + inosine
CDP + inosine 5'-phosphate
show the reaction diagram
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
-
-
?
GTP + inosine
GDP + inosine 5'-phosphate
show the reaction diagram
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
-
-
?
ITP + inosine
IDP + inosine 5'-phosphate
show the reaction diagram
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
-
-
?
TTP + inosine
TDP + inosine 5'-phosphate
show the reaction diagram
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
-
-
?
UTP + inosine
UDP + inosine 5'-phosphate
show the reaction diagram
phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%)
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
enzyme activity requires the presence of divalent cations. Mn2+ is the most efficient, followed by Mg2+, Ni2+ and Co2+
Mg2+
enzyme activity requires the presence of divalent cations. Mn2+ is the most efficient, followed by Mg2+, Ni2+ and Co2+. Maximum activity is attained when the ATP/Mg2+ concentration ratio is 0.5
Ni2+
enzyme activity requires the presence of divalent cations. Mn2+ is the most efficient, followed by Mg2+, Ni2+ and Co2+
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
adenosine
concentrations above 0.1 mM lead to reduction in enzyme activity
Inosine
concentrations above 0.1 mM lead to reduction in enzyme activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.1
2'-deoxyadenosine
pH 7.0, 37°C
0.02
adenosine
pH 7.0, 37°C
0.6 - 3
ATP
pH 7.0, 37°C
0.46
guanosine
pH 7.0, 37°C
0.15
Inosine
pH 7.0, 37°C
0.87
mizoribine
pH 7.0, 37°C
0.79
ribavirin
pH 7.0, 37°C
1.7
uridine
pH 7.0, 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.84
2'-deoxyadenosine
pH 7.0, 37°C
6.05
adenosine
pH 7.0, 37°C
0.24
guanosine
pH 7.0, 37°C
80
Inosine
pH 7.0, 37°C
0.04
mizoribine
pH 7.0, 37°C
0.08
ribavirin
pH 7.0, 37°C
0.28
uridine
pH 7.0, 37°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q2SZE4_BURTA
Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)
312
0
34013
TrEMBL
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34000
2 * 34000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
2 * 34000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 10
37°C, 3 h, stable
697882
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
pH 7.0, 20 min, stable
60
pH 7.0, 20 min, 60% loss of activity
70
pH 7.0, 20 min, complete inactivation
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
functionally overexpressed in Rhodococcus erythropolis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ota, H.; Sakasegawa, S.; Yasuda, Y.; Imamura, S.; Tamura, T.
A novel nucleoside kinase from Burkholderia thailandensis: a member of the phosphofructokinase B-type family of enzymes
FEBS J.
275
5865-5872
2008
Burkholderia thailandensis (Q2SZE4), Burkholderia thailandensis, Burkholderia thailandensis DSM 13276 (Q2SZE4)
Manually annotated by BRENDA team