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Information on EC 2.7.1.83 - pseudouridine kinase and Organism(s) Escherichia coli and UniProt Accession P30235

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Escherichia coli
UNIPROT: P30235 not found.
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The taxonomic range for the selected organisms is: Escherichia coli
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
pseudouridine kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
kinase, pseudouridine (phosphorylating)
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:pseudouridine 5'-phosphotransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
62213-40-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + pseudouridine
ADP + pseudouridine 5'-phosphate
show the reaction diagram
ATP + pseudouridine
ADP + pseudouridine 5'-monophosphate
show the reaction diagram
-
-
-
?
ATP + pseudouridine
ADP + pseudouridine-5'-monophosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + pseudouridine
ADP + pseudouridine 5'-phosphate
show the reaction diagram
two enzymes are involved in the metabolism of pseudouridine in uropathogenic Escherichia coli, the principal agent of urinary tract infections in humans. The first enzyme, coded by the gene yeiC, specifically phosphorylates pseudouridine to pseudouridine 5'-phosphate. yeiC- mutants are unable to metabolize pseudouridine, in contrast to wild-type Escherichia coli UTI89. The second enzyme, encoded by the gene yeiN (EC 4.2.1.70) belonging to the same operon as yeiC, catalyzes the conversion of pseudouridine 5'-phosphate to uracil and ribose 5-phosphate in a divalent cation-dependent manner
-
-
?
ATP + pseudouridine
ADP + pseudouridine-5'-monophosphate
show the reaction diagram
-
involved in utilization of pseudouridine as pyrimidine source by E. coli pyrimidine auxotrophs
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
enzyme is dependent on the presence of Mg2+
K+
-
requirement
NH4+
-
can partially substitute for K+
additional information
-
Li+, Na+ are ineffective
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.33
ATP
pH 7.1, 30°C
0.12
pseudouridine
pH 7.1, 30°C
0.33 - 2.9
ATP
3.3
K+
-
pH 7.0, 37°C
40
NH4+
-
pH 7.0, 37°C
0.12 - 0.18
pseudouridine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain UTI89
SwissProt
Manually annotated by BRENDA team
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, partially purified
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Solomon, L.R.; Breitmann, T.R.
Pseudouridine kinase of Escherichia coli: a new enzyme
Biochem. Biophys. Res. Commun.
44
299-304
1971
Escherichia coli, Escherichia coli Bu-
Manually annotated by BRENDA team
Preumont, A.; Snoussi, K.; Stroobant, V.; Collet, J.F.; Van Schaftingen, E.
Molecular identification of pseudouridine-metabolizing enzymes
J. Biol. Chem.
283
25238-25246
2008
Escherichia coli, Escherichia coli (P30235)
Manually annotated by BRENDA team