Information on EC 2.7.1.83 - pseudouridine kinase

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The expected taxonomic range for this enzyme is: Escherichia coli

EC NUMBER
COMMENTARY hide
2.7.1.83
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RECOMMENDED NAME
GeneOntology No.
pseudouridine kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + pseudouridine = ADP + pseudouridine 5'-phosphate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
pseudouridine degradation
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Pyrimidine metabolism
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SYSTEMATIC NAME
IUBMB Comments
ATP:pseudouridine 5'-phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
62213-40-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
and B5Ru
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + pseudouridine
ADP + pseudouridine 5'-monophosphate
show the reaction diagram
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-
-
?
ATP + pseudouridine
ADP + pseudouridine 5'-phosphate
show the reaction diagram
ATP + pseudouridine
ADP + pseudouridine-5'-monophosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + pseudouridine
ADP + pseudouridine 5'-phosphate
show the reaction diagram
P30235
two enzymes are involved in the metabolism of pseudouridine in uropathogenic Escherichia coli, the principal agent of urinary tract infections in humans. The first enzyme, coded by the gene yeiC, specifically phosphorylates pseudouridine to pseudouridine 5'-phosphate. yeiC- mutants are unable to metabolize pseudouridine, in contrast to wild-type Escherichia coli UTI89. The second enzyme, encoded by the gene yeiN (EC 4.2.1.70) belonging to the same operon as yeiC, catalyzes the conversion of pseudouridine 5'-phosphate to uracil and ribose 5-phosphate in a divalent cation-dependent manner
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?
ATP + pseudouridine
ADP + pseudouridine-5'-monophosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
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requirement
NH4+
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can partially substitute for K+
additional information
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Li+, Na+ are ineffective
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.33 - 2.9
ATP
3.3
K+
-
pH 7.0, 37°C
40
NH4+
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pH 7.0, 37°C
0.12 - 0.18
pseudouridine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, partially purified
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Show AA Sequence (334 entries)
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