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Information on EC 2.7.1.73 - inosine kinase and Organism(s) Escherichia coli and UniProt Accession P0AEW6

for references in articles please use BRENDA:EC2.7.1.73
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This record set is specific for:
Escherichia coli
UNIPROT: P0AEW6 not found.
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The taxonomic range for the selected organisms is: Escherichia coli
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Reaction Schemes
Synonyms
inosine kinase, inosine-guanosine kinase, guanosine-inosine kinase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
guanosine-inosine kinase
inosine-guanosine kinase
-
-
-
-
kinase, inosine (phosphorylating)
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + inosine = ADP + IMP
show the reaction diagram
ordered bi bi mechanism in which guanosine is the first substrate to bind and GMP is the last product to be released
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
ATP:inosine 5'-phosphotransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
37237-46-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + guanosine
ADP + GMP
show the reaction diagram
-
-
-
?
ATP + inosine
ADP + 5'-IMP
show the reaction diagram
about 60% of the activity with guanosine
-
?
dATP + guanosine
dADP + GMP
show the reaction diagram
-
-
-
?
dATP + inosine
dADP + IMP
show the reaction diagram
-
-
-
?
UTP + guanosine
UDP + GMP
show the reaction diagram
UTP shows 20% of the activity with ATP
-
-
?
UTP + inosine
UDP + IMP
show the reaction diagram
UTP shows 20% of the activity with ATP
-
-
?
ATP + deoxyguanosine
ADP + dGMP
show the reaction diagram
-
-
-
-
?
ATP + guanosine
ADP + GMP
show the reaction diagram
-
-
-
-
?
ATP + inosine
ADP + 5'-IMP
show the reaction diagram
-
-
-
-
?
ATP + inosine
ADP + IMP
show the reaction diagram
-
-
-
-
?
ATP + xanthosine
ADP + XMP
show the reaction diagram
-
-
-
-
?
CTP + inosine
CDP + IMP
show the reaction diagram
-
-
-
-
?
dATP + inosine
dADP + IMP
show the reaction diagram
-
-
-
-
?
UTP + inosine
UDP + IMP
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + guanosine
ADP + GMP
show the reaction diagram
-
-
-
-
?
ATP + inosine
ADP + IMP
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
required
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
K+
the enzyme requires both Mg2+ and K+
Mg2+
enzyme requires both Mg2+ and K+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.51 - 0.71
ATP
0.66 - 2.4
dATP
0.0061
guanosine
pH 7.2, 30°C
2.1
Inosine
pH 7.2, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.9
inosine kinase
8.2
guanosine kinase
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
26 - 39
inosine kinase
38
guanosine kinase
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
isoelectric focusing
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
in the salvage pathway, guanosine and inosine are directly phosphorylated to GMP and IMP, respectively, by guanosine-inosine kinase, or guanosine is indirectly converted to GMP through serial phosphorolysis and phosphorylation catalyzed by purine nucleoside phosphorylase and guanine phosphoribosyltransferase, respectively, pathway, overview
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48400
x * 48400, calculation from nucleotide sequence
45000
-
2 * 45000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 48400, calculation from nucleotide sequence
dimer
-
2 * 45000
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
overexpression of Gsk in the fed-batch fermentation in recombinant Escherichia coli strain BL21star(DE3) with glucose as the sole carbon source, leads to a significant improvement of GDP-L-fucose production, profiles of cell growth and GDP-L-fucose production, overview
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene gsk, several metabolic enzymes such as GMP synthetase and IMP dehydrogenase, GMP reductase, and guanosine-inosine kinase, all involved in the biosynthesis of GMP, are co-overexpressed in recombinant Escherichia coli TOP10 and BL21star(DE3) cells becoming able to produce GDP-L-fucose, pH-stat fed-batch fermentations quantification and optimization, overview
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mori, H.; Iida, A.; Teshiba, S.; Fujio, T.
Cloning of a guanosine-inosine kinase gene of Escherichia coli and characterization of the purified gene product
J. Bacteriol.
177
4921-4926
1995
Escherichia coli (P0AEW6), Escherichia coli
Manually annotated by BRENDA team
Kawasaki, H.; Shimaoka, M.; Usuda, Y.; Utagawa, T.
End-product regulation and kinetic mechanism of guanosine-inosine kinase from Escherichia coli
Biosci. Biotechnol. Biochem.
64
972-979
2000
Escherichia coli
Manually annotated by BRENDA team
Lee, W.H.; Shin, S.Y.; Kim, M.D.; Han, N.S.; Seo, J.H.
Modulation of guanosine nucleotides biosynthetic pathways enhanced GDP-L-fucose production in recombinant Escherichia coli
Appl. Microbiol. Biotechnol.
93
2327-2334
2012
Escherichia coli
Manually annotated by BRENDA team