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Information on EC 2.7.1.68 - 1-phosphatidylinositol-4-phosphate 5-kinase and Organism(s) Arabidopsis thaliana and UniProt Accession O48709

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IUBMB Comments
This enzyme can also phosphorylate PtdIns3P in the 4-position, and PtdIns, PtdIns3P and PtdIns(3,4)P2 in the 5-position in vitro, but to a lesser extent. The last of these reactions occurs in vivo and is physiologically relevant. Three different isoforms are known.
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Select one or more organisms in this record:
This record set is specific for:
Arabidopsis thaliana
UNIPROT: O48709
Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
Diphosphoinositide kinase, More, Mss4, MSS4p, phosphatidylinositol 4,5-biphosphate kinase, phosphatidylinositol 4-phosphate 5-kinase, phosphatidylinositol 4-phosphate 5-kinase alpha, phosphatidylinositol 4-phosphate 5-kinase beta, phosphatidylinositol 4-phosphate 5-kinase gamma, phosphatidylinositol 4-phosphate 5-kinase gamma661, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Diphosphoinositide kinase
-
-
-
-
MSS4p
-
-
-
-
phosphatidylinositol 4-phosphate 5-kinase
phosphatidylinositol 4-phosphate kinase
-
-
-
-
phosphatidylinositol monophosphate 5-kinase
281303
-
phosphatidylinositol phosphate 5-kinase
phosphatidylinositol phosphate 5-kinase 1
277850
-
phosphatidylinositol phosphate 5-kinase 10
277848
-
phosphatidylinositol-4-phosphate 5-kinase
-
-
-
-
PI4P 5-kinase
-
-
-
-
PI4P5K
-
-
-
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PIP kinase
-
-
-
-
PIP-5kin
-
-
-
-
PIP5K
PIP5K1
302
isoform
PIP5K2
302
isoform
PIP5K3
284899
PIP5K3 encodes the enzyme activity producing PtdIns(4,5)P2.
PIP5K9
281303
-
PIP5Ks
-
-
-
-
PIPK1
277850
-
PIPK10
277848
-
PtdIns(4)P 5-kinase
-
-
-
-
PtdInsP
277848, 277850
-
type I PtdInsP kinase
284899
-
additional information
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Phosphorylation
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phospho group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:1-phosphatidyl-1D-myo-inositol-4-phosphate 5-phosphotransferase
This enzyme can also phosphorylate PtdIns3P in the 4-position, and PtdIns, PtdIns3P and PtdIns(3,4)P2 in the 5-position in vitro, but to a lesser extent. The last of these reactions occurs in vivo and is physiologically relevant. Three different isoforms are known.
CAS REGISTRY NUMBER
COMMENTARY hide
104645-76-3
-
9032-61-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
ADP + 1-phosphatidyl-1D-myo-inositol-4,5-bisphosphate
show the reaction diagram
-
PIP5K3 is involved in localizing 1-phosphatidyl-1D-myo-inositol-4,5-bisphosphate to the elongating root hair apex and is a key regulator of the machinery that initiates and promotes root hair tip growth.
-
r
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
ADP + 1-phosphatidyl-1D-myo-inositol-4,5-bisphosphate
show the reaction diagram
O48709
-
PIP5K3 is involved in localizing 1-phosphatidyl-1D-myo-inositol-4,5-bisphosphate to the elongating root hair apex and is a key regulator of the machinery that initiates and promotes root hair tip growth.
-
r
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
70 mM in the activity assay
Mg2+
10 mM in the activity assay
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
phosphatidic acid
activates enzyme form 1 by 2.1fold
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.065 - 0.6
1-phosphatidyl-1D-myo-inositol 4-phosphate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Used Arabidopsis thaliana lines are in the Columbia background, and Columbia is used as the wild type.
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Escherichia coli BL21 cell is used for cloning and expression
Manually annotated by BRENDA team
t-DNA insertion mutations that reduces the expression of PIP5K3 cause shorter root hairs than in the wild type. Overexpression causes longer root hairs and multiple protruding sites on a single trichoblast.
Manually annotated by BRENDA team
enzyme form 10, most abundant in
Manually annotated by BRENDA team
-
enzyme localizes to the periphery of the apical region of root hair cells
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
cytoplasmic space of elongating root hair apices
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
PI5K3_ARATH
705
0
80134
Swiss-Prot
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
Partial purification from Escherichia coli culture cells. The cells are suspended in PBS buffer, sonicated on ice, and centrifuged. Supernatant is mixed with glutathione-Sepharose 4B and incubated at 48°C for 2 h. The Sepharose beads are collected and washed with an excess volume of PBS.
recombinant GST-fusion protein from Escherichia coli in presence of Triton X-100 and PtdOH
CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
a yellow fluorescent (YFP) und GUS fusion PIP5K3 protein is expressed in Escherichia coli cells and transformed in Arabidopsis thaliana to produce trangenic plants. Overexpression and mutant is investigated.
expressed in Nicotiana tabacum
expression in Escherichia coli
-
expression of enzyme form 1 as GST-fusion protein in Escherichia coli, expression of His-tagged enzyme form 1 in Spodoptera frugiperda Sf9 cells via baculovirus infection system
expression of enzyme form 10 as GST-fusion protein in Escherichia coli, expression of His-tagged enzyme form 10 in Spodoptera frugiperda Sf9 cells via baculovirus infection system
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the enzyme transcript levels increase in response to auxin and indole 3-acetic acid
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mikami, K.; Katagiri, T.; Iuchi, S.; Yamaguchi-Shinozaki, K.; Shinozaki, K.
A gene encoding phosphatidylinositol-4-phosphate 5-kinase is induced by water stress and abscisic acid in Arabidopsis thaliana
Plant J.
15
563-568
1998
Arabidopsis thaliana
Manually annotated by BRENDA team
Perera, I.Y.; Davis, A.J.; Galanopoulou, D.; Im, Y.J.; Boss, W.F.
Characterization and comparative analysis of Arabidopsis phosphatidylinositol phosphate 5-kinase 10 reveals differences in Arabidopsis and human phosphatidylinositol phosphate kinases
FEBS Lett.
579
3427-3432
2005
Arabidopsis thaliana (Q56YP2), Arabidopsis thaliana (Q9LMN1), Homo sapiens, Homo sapiens (Q99755)
Manually annotated by BRENDA team
Lou, Y.; Gou, J.Y.; Xue, H.W.
PIP5K9, an Arabidopsis phosphatidylinositol monophosphate kinase, interacts with a cytosolic invertase to negatively regulate sugar-mediated root growth
Plant Cell
19
163-181
2007
Arabidopsis thaliana, Arabidopsis thaliana (Q8L850)
Manually annotated by BRENDA team
Kusano, H.; Testerink, C.; Vermeer, J.E.; Tsuge, T.; Shimada, H.; Oka, A.; Munnik, T.; Aoyama, T.
The arabidopsis phosphatidylinositol phosphate 5-kinase PIP5K3 is a key regulator of root hair tip growth
Plant Cell
20
367-380
2008
Arabidopsis thaliana, Arabidopsis thaliana (O48709)
Manually annotated by BRENDA team
Stenzel, I.; Ischebeck, T.; Koenig, S.; Ho?ubowska, A.; Sporysz, M.; Hause, B.; Heilmann, I.
The type B phosphatidylinositol-4-phosphate 5-kinase 3 is essential for root hair formation in Arabidopsis thaliana
Plant Cell
20
124-141
2008
Arabidopsis thaliana
Manually annotated by BRENDA team
Wada, Y.; Kusano, H.; Tsuge, T.; Aoyama, T.
Phosphatidylinositol phosphate 5-kinase genes respond to phosphate deficiency for root hair elongation in Arabidopsis thaliana
Plant J.
81
426-437
2015
Arabidopsis thaliana
Manually annotated by BRENDA team
Ugalde, J.M.; Rodriguez-Furlan, C.; Rycke, R.D.; Norambuena, L.; Friml, J.; Leon, G.; Tejos, R.
Phosphatidylinositol 4-phosphate 5-kinases 1 and 2 are involved in the regulation of vacuole morphology during Arabidopsis thaliana pollen development
Plant Sci.
250
10-19
2016
Arabidopsis thaliana
Manually annotated by BRENDA team
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