Information on EC 2.7.1.64 - inositol 3-kinase
for references in articles please use BRENDA:EC2.7.1.64
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EC Tree
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
inositol 1-kinase, kinase, inositol 1- (phosphorylating), MI3K, MIK, myo-inositol 3-kinase, myo-inositol kinase, myoinositol kinase, TK2285 protein,
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inositol 1-kinase
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kinase, inositol 1- (phosphorylating)
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-
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myoinositol kinase
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-
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myo-inositol 3-kinase
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-
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myo-inositol kinase
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ATP + myo-inositol = ADP + 1D-myo-inositol 3-phosphate
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-
-
-
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phospho group transfer
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-
-
-
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ATP:myo-inositol 1-phosphotransferase
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ATP + 1D-myo-inositol
ADP + 1D-myo-inositol 1-phosphate
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-
-
-
?
ATP + D-allose
?
-
-
-
-
?
ATP + D-lyxose
?
-
-
-
-
?
ATP + D-ribose
?
-
-
-
-
?
ATP + D-tagatose
?
-
-
-
-
?
ATP + D-talose
?
-
-
-
-
?
ATP + D-xylose
?
-
-
-
-
?
ATP + D-xylulose
?
-
-
-
-
?
ATP + myo-inositol
ADP + 1D-myo-inositol 3-phosphate
ATP + myo-inositol
ADP + 1L-myo-inositol 1-phosphate
-
-
-
?
ATP + myo-inositol
ADP + myo-inositol 1-phosphate
CTP + myo-inositol
CDP + myo-inositol 1-phosphate
GTP + myo-inositol
GDP + myo-inositol 1-phosphate
UTP + myo-inositol
UDP + myo-inositol 1-phosphate
additional information
?
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no activity with glycerol, DL-threitol, L-iditol, dulcitol, volemitol, D-lactitol monohydrate, maltitol, D-glucosamine, N-acetyl-D-glucosamine, maltose and lactose, D-erythrose, D-threose, D-erythrulose, L-arabinose, L-fructose, L-glucose, D-ribulose, D-galactose, fructose 1-phosphate, fructose 6-phosphate, glucose 1-phosphate, glucose 6-phosphate, ribose 1-phosphate, ribose 5-phosphate, tagatose 6-phosphate, adenosine, uridine, guanosine, cytidine, thymidine, and inosine
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ATP + myo-inositol
ADP + 1D-myo-inositol 3-phosphate
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-
-
?
ATP + myo-inositol
ADP + 1D-myo-inositol 3-phosphate
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-
-
-
?
ATP + myo-inositol
ADP + myo-inositol 1-phosphate
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-
-
?
ATP + myo-inositol
ADP + myo-inositol 1-phosphate
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-
-
?
ATP + myo-inositol
ADP + myo-inositol 1-phosphate
-
-
-
?
ATP + myo-inositol
ADP + myo-inositol 1-phosphate
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-
-
?
ATP + myo-inositol
ADP + myo-inositol 1-phosphate
-
biosynthesis of phytic acid
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-
-
ATP + myo-inositol
ADP + myo-inositol 1-phosphate
-
-
-
?
ATP + myo-inositol
ADP + myo-inositol 1-phosphate
-
-
-
?
ATP + myo-inositol
ADP + myo-inositol 1-phosphate
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-
-
?
ATP + myo-inositol
ADP + myo-inositol 1-phosphate
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-
-
?
ATP + myo-inositol
ADP + myo-inositol 1-phosphate
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-
-
?
CTP + myo-inositol
CDP + myo-inositol 1-phosphate
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weak activity
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?
CTP + myo-inositol
CDP + myo-inositol 1-phosphate
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weak activity
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?
CTP + myo-inositol
CDP + myo-inositol 1-phosphate
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weak activity
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?
CTP + myo-inositol
CDP + myo-inositol 1-phosphate
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weak activity
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?
GTP + myo-inositol
GDP + myo-inositol 1-phosphate
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no actiity with TTP
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?
GTP + myo-inositol
GDP + myo-inositol 1-phosphate
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no actiity with TTP
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?
GTP + myo-inositol
GDP + myo-inositol 1-phosphate
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no actiity with TTP
-
?
GTP + myo-inositol
GDP + myo-inositol 1-phosphate
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no actiity with TTP
-
?
UTP + myo-inositol
UDP + myo-inositol 1-phosphate
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-
-
?
UTP + myo-inositol
UDP + myo-inositol 1-phosphate
-
-
-
?
UTP + myo-inositol
UDP + myo-inositol 1-phosphate
-
-
-
?
UTP + myo-inositol
UDP + myo-inositol 1-phosphate
-
-
-
?
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ATP + myo-inositol
ADP + 1D-myo-inositol 3-phosphate
ATP + myo-inositol
ADP + myo-inositol 1-phosphate
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biosynthesis of phytic acid
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-
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ATP + myo-inositol
ADP + 1D-myo-inositol 3-phosphate
Q5JDA3
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-
-
?
ATP + myo-inositol
ADP + 1D-myo-inositol 3-phosphate
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-
-
-
?
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Co2+
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133% activity at 3 mM
Mg2+
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required for activity
Zn2+
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125% activity at 3 mM
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Ca2+
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49% residual activity at 3 mM
Mn2+
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21% residual activity at 3 mM
Ni2+
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53% residual activity at 3 mM
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0.0531
1D-myo-inositol
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in 20 mM HEPES (pH 7.2), 6 mM MgCl2, 10 mM LiCl, 1 mM dithiothreitol, at 30°C
0.036
ATP
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in 20 mM HEPES (pH 7.2), 6 mM MgCl2, 10 mM LiCl, 1 mM dithiothreitol, at 30°C
863
D-allose
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at pH 7.5 and 85°C
171
D-Lyxose
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at pH 7.5 and 85°C
951
D-ribose
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at pH 7.5 and 85°C
53
D-tagatose
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at pH 7.5 and 85°C
438
D-talose
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at pH 7.5 and 85°C
418
D-xylose
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at pH 7.5 and 85°C
524
D-xylulose
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at pH 7.5 and 85°C
0.36
myo-inositol
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at pH 7.5 and 85°C
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18.3
D-allose
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at pH 7.5 and 85°C
3.9
D-Lyxose
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at pH 7.5 and 85°C
5.1
D-ribose
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at pH 7.5 and 85°C
1.5
D-tagatose
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at pH 7.5 and 85°C
13.1
D-talose
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at pH 7.5 and 85°C
5.6
D-xylose
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at pH 7.5 and 85°C
11.6
D-xylulose
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at pH 7.5 and 85°C
23.2
myo-inositol
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at pH 7.5 and 85°C
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0.021
D-allose
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at pH 7.5 and 85°C
0.023
D-Lyxose
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at pH 7.5 and 85°C
0.005
D-ribose
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at pH 7.5 and 85°C
0.028
D-tagatose
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at pH 7.5 and 85°C
0.03
D-talose
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at pH 7.5 and 85°C
0.013
D-xylose
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at pH 7.5 and 85°C
0.022
D-xylulose
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at pH 7.5 and 85°C
64.9
myo-inositol
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at pH 7.5 and 85°C
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0.063
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with meso-erythritol as substrate, at pH 7.5 and 85°C
0.098
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with L-arabitol as substrate, at pH 7.5 and 85°C
0.17
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with cis-inositol as substrate, at pH 7.5 and 85°C
0.24
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with L-chiro-inositol as substrate, at pH 7.5 and 85°C
0.39
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with adonitol as substrate, at pH 7.5 and 85°C
0.5
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with D-arabitol as substrate, at pH 7.5 and 85°C
1.06
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with myo-inositol 1-phosphate as substrate, at pH 7.5 and 85°C
1.91
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with allo-inositol as substrate, at pH 7.5 and 85°C
17.64
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with scyllo-inositol as substrate, at pH 7.5 and 85°C
34.92
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with D-chiro-inositol as substrate, at pH 7.5 and 85°C
44.27
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with myo-inositol as substrate, at pH 7.5 and 85°C
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7.8
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-
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6.7 - 8.7
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approx. 25% of maximal activity at pH 6.7, approx. 75% of maximal activity at pH 8.7
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brenda
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brenda
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brenda
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brenda
spinach
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brenda
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UniProt
brenda
wheat
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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MIK_ARATH
353
0
38646
Swiss-Prot
MIK_MAIZE
379
0
39898
Swiss-Prot
MIK_ORYSJ
397
0
41540
Swiss-Prot
A0A2P6PIK1_ROSCH
90
0
10192
TrEMBL
A0A2P6PZ89_ROSCH
58
0
6326
TrEMBL
A0A2P6PS81_ROSCH
164
1
18239
TrEMBL
A0A2P6QJX7_ROSCH
385
0
42092
TrEMBL
G7KE51_MEDTR
372
0
40686
TrEMBL
Q5JDA3_THEKO
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
273
0
30309
TrEMBL
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Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
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?
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x * 30300, calculated from amino acid sequence
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complexed with an ATP analogue and myo-inositol, hanging drop vapor diffusion method, using
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sitting drop vapor diffusion method, usingv 18-23% (w/v) polyethylene glycol 3350 and 0.2 M NH4I
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65 - 95
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the enzyme is extremely thermostable with half-lives of 3 h at 95°C, 19 h at 85°C, 40 h at 75°C, and 88 h at 65°C
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ammonium sulfate, density-gradient centrifugation, partial purification
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ammonium sulfate, partial purification
Resource Q column chromatography, Resource PHE hydrophobic column chromatography, and Superdex 200 gel filtration
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ammonium sulfate, partial purification
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ammonium sulfate, partial purification
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expressed in Escherichia coli
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expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL and Rosetta2(DE3)pLysS cells
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English, P.D.; Dietz, M.; Albersheim, P.
Myoinositol kinase: partial purification and identification of product
Science
151
198-199
1966
Spinacia oleracea, Triticum aestivum, Vigna radiata var. radiata
brenda
Molinari, E.; Hoffmann-Ostenhof, O.
Studies on the biosynthesis of cyclitols. XXI. An enzyme system capable of phosphorylating myo-inositol to phytic acid
Hoppe-Seyler's Z. Physiol. Chem.
349
1797-1799
1968
Lemna gibba
brenda
Loewus, M.W.; Sasaki, K.; Leavitt, A.L.; Munsell, L.; Sherman, W.R.; Loewus, F.A.
Enantiomeric form of myo-inositol-1-phosphate produced by myo-inositol-1-phosphate synthase and myo-inositol kinase in higher plants
Plant Physiol.
70
1661-1663
1982
Triticum aestivum
brenda
Dietz, M.; Albersheim, P.
The enzymic phosphorylation of myo-inositol
Biochem. Biophys. Res. Commun.
19
598-603
1965
Acer pseudoplatanus, Bos taurus, Escherichia coli, Vigna radiata var. radiata
brenda
Shi, J.; Wang, H.; Hazebroek, J.; Ertl, D.S.; Harp, T.
The maize low-phytic acid 3 encodes a myo-inositol kinase that plays a role in phytic acid biosynthesis in developing seeds
Plant J.
42
708-719
2005
Zea mays
brenda
Nagata, R.; Fujihashi, M.; Sato, T.; Atomi, H.; Miki, K.
Crystal structure and product analysis of an archaeal myo-inositol kinase reveal substrate recognition mode and 3-OH phosphorylation
Biochemistry
54
3494-3503
2015
Thermococcus kodakarensis (Q5JDA3), Thermococcus kodakarensis
brenda
Sato, T.; Fujihashi, M.; Miyamoto, Y.; Kuwata, K.; Kusaka, E.; Fujita, H.; Miki, K.; Atomi, H.
An uncharacterized member of the ribokinase family in Thermococcus kodakarensis exhibits myo-inositol kinase activity
J. Biol. Chem.
288
20856-20867
2013
Thermococcus kodakarensis (Q5JDA3), Thermococcus kodakarensis
brenda
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2.7.1.64 inositol 3-kinase
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