Information on EC 2.7.1.64 - inositol 3-kinase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

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EC NUMBER
COMMENTARY hide
2.7.1.64
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RECOMMENDED NAME
GeneOntology No.
inositol 3-kinase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + myo-inositol = ADP + 1D-myo-inositol 3-phosphate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
1D-myo-inositol hexakisphosphate biosynthesis III (Spirodela polyrrhiza)
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1D-myo-inositol hexakisphosphate biosynthesis IV (Dictyostelium)
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Inositol phosphate metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
ATP:myo-inositol 1-phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
37278-07-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
spinach
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Manually annotated by BRENDA team
wheat
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 1D-myo-inositol
ADP + 1D-myo-inositol 1-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-allose
?
show the reaction diagram
-
-
-
-
?
ATP + D-lyxose
?
show the reaction diagram
-
-
-
-
?
ATP + D-ribose
?
show the reaction diagram
-
-
-
-
?
ATP + D-tagatose
?
show the reaction diagram
-
-
-
-
?
ATP + D-talose
?
show the reaction diagram
-
-
-
-
?
ATP + D-xylose
?
show the reaction diagram
-
-
-
-
?
ATP + D-xylulose
?
show the reaction diagram
-
-
-
-
?
ATP + myo-inositol
ADP + 1D-myo-inositol 3-phosphate
show the reaction diagram
ATP + myo-inositol
ADP + 1L-myo-inositol 1-phosphate
show the reaction diagram
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-
-
?
ATP + myo-inositol
ADP + myo-inositol 1-phosphate
show the reaction diagram
CTP + myo-inositol
CDP + myo-inositol 1-phosphate
show the reaction diagram
GTP + myo-inositol
GDP + myo-inositol 1-phosphate
show the reaction diagram
UTP + myo-inositol
UDP + myo-inositol 1-phosphate
show the reaction diagram
additional information
?
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no activity with glycerol, DL-threitol, L-iditol, dulcitol, volemitol, D-lactitol monohydrate, maltitol, D-glucosamine, N-acetyl-D-glucosamine, maltose and lactose, D-erythrose, D-threose, D-erythrulose, L-arabinose, L-fructose, L-glucose, D-ribulose, D-galactose, fructose 1-phosphate, fructose 6-phosphate, glucose 1-phosphate, glucose 6-phosphate, ribose 1-phosphate, ribose 5-phosphate, tagatose 6-phosphate, adenosine, uridine, guanosine, cytidine, thymidine, and inosine
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + myo-inositol
ADP + 1D-myo-inositol 3-phosphate
show the reaction diagram
ATP + myo-inositol
ADP + myo-inositol 1-phosphate
show the reaction diagram
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biosynthesis of phytic acid
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
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133% activity at 3 mM
Mg2+
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required for activity
Zn2+
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125% activity at 3 mM
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
-
49% residual activity at 3 mM
Mn2+
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21% residual activity at 3 mM
Ni2+
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53% residual activity at 3 mM
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0531
1D-myo-inositol
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in 20 mM HEPES (pH 7.2), 6 mM MgCl2, 10 mM LiCl, 1 mM dithiothreitol, at 30°C
0.036
ATP
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in 20 mM HEPES (pH 7.2), 6 mM MgCl2, 10 mM LiCl, 1 mM dithiothreitol, at 30°C
863
D-Allose
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at pH 7.5 and 85°C
171
D-Lyxose
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at pH 7.5 and 85°C
951
D-ribose
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at pH 7.5 and 85°C
53
D-tagatose
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at pH 7.5 and 85°C
438
D-talose
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at pH 7.5 and 85°C
418
D-xylose
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at pH 7.5 and 85°C
524
D-xylulose
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at pH 7.5 and 85°C
0.36
myo-inositol
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at pH 7.5 and 85°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
18.3
D-Allose
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at pH 7.5 and 85°C
3.9
D-Lyxose
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at pH 7.5 and 85°C
5.1
D-ribose
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at pH 7.5 and 85°C
1.5
D-tagatose
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at pH 7.5 and 85°C
13.1
D-talose
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at pH 7.5 and 85°C
5.6
D-xylose
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at pH 7.5 and 85°C
11.6
D-xylulose
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at pH 7.5 and 85°C
23.2
myo-inositol
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at pH 7.5 and 85°C
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.021
D-Allose
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at pH 7.5 and 85°C
0.023
D-Lyxose
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at pH 7.5 and 85°C
0.005
D-ribose
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at pH 7.5 and 85°C
0.028
D-tagatose
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at pH 7.5 and 85°C
0.03
D-talose
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at pH 7.5 and 85°C
0.013
D-xylose
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at pH 7.5 and 85°C
0.022
D-xylulose
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at pH 7.5 and 85°C
64.9
myo-inositol
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at pH 7.5 and 85°C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.063
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with meso-erythritol as substrate, at pH 7.5 and 85°C
0.098
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with L-arabitol as substrate, at pH 7.5 and 85°C
0.17
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with cis-inositol as substrate, at pH 7.5 and 85°C
0.24
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with L-chiro-inositol as substrate, at pH 7.5 and 85°C
0.39
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with adonitol as substrate, at pH 7.5 and 85°C
0.5
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with D-arabitol as substrate, at pH 7.5 and 85°C
1.06
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with myo-inositol 1-phosphate as substrate, at pH 7.5 and 85°C
1.91
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with allo-inositol as substrate, at pH 7.5 and 85°C
17.64
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with scyllo-inositol as substrate, at pH 7.5 and 85°C
34.92
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with D-chiro-inositol as substrate, at pH 7.5 and 85°C
44.27
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with myo-inositol as substrate, at pH 7.5 and 85°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.7 - 8.7
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approx. 25% of maximal activity at pH 6.7, approx. 75% of maximal activity at pH 8.7
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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PDB
SCOP
CATH
ORGANISM
UNIPROT
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 30300, calculated from amino acid sequence
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Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
complexed with an ATP analogue and myo-inositol, hanging drop vapor diffusion method, using
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sitting drop vapor diffusion method, usingv 18-23% (w/v) polyethylene glycol 3350 and 0.2 M NH4I
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65 - 95
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the enzyme is extremely thermostable with half-lives of 3 h at 95°C, 19 h at 85°C, 40 h at 75°C, and 88 h at 65°C
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, density-gradient centrifugation, partial purification
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ammonium sulfate, partial purification
Resource Q column chromatography, Resource PHE hydrophobic column chromatography, and Superdex 200 gel filtration
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL and Rosetta2(DE3)pLysS cells
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LINKS TO OTHER DATABASES (specific for EC-Number 2.7.1.64)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)