Information on EC 2.7.1.64 - inositol 3-kinase

for references in articles please use BRENDA:EC2.7.1.64
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

top print hide
EC NUMBER
COMMENTARY hide
2.7.1.64
-
top print hide
RECOMMENDED NAME
GeneOntology No.
inositol 3-kinase
top print hide
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + myo-inositol = ADP + 1D-myo-inositol 3-phosphate
show the reaction diagram
-
-
-
-
top print hide
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
top print hide
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
1D-myo-inositol hexakisphosphate biosynthesis III (Spirodela polyrrhiza)
-
-
1D-myo-inositol hexakisphosphate biosynthesis IV (Dictyostelium)
-
-
Inositol phosphate metabolism
-
-
Metabolic pathways
-
-
top print hide
SYSTEMATIC NAME
IUBMB Comments
ATP:myo-inositol 1-phosphotransferase
-
top
top print hide
CAS REGISTRY NUMBER
COMMENTARY hide
37278-07-2
-
top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
spinach
-
-
Manually annotated by BRENDA team
wheat
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
top print hide
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 1D-myo-inositol
ADP + 1D-myo-inositol 1-phosphate
show the reaction diagram
-
-
-
-
?
ATP + D-allose
?
show the reaction diagram
-
-
-
-
?
ATP + D-lyxose
?
show the reaction diagram
-
-
-
-
?
ATP + D-ribose
?
show the reaction diagram
-
-
-
-
?
ATP + D-tagatose
?
show the reaction diagram
-
-
-
-
?
ATP + D-talose
?
show the reaction diagram
-
-
-
-
?
ATP + D-xylose
?
show the reaction diagram
-
-
-
-
?
ATP + D-xylulose
?
show the reaction diagram
-
-
-
-
?
ATP + myo-inositol
ADP + 1D-myo-inositol 3-phosphate
show the reaction diagram
ATP + myo-inositol
ADP + 1L-myo-inositol 1-phosphate
show the reaction diagram
-
-
-
?
ATP + myo-inositol
ADP + myo-inositol 1-phosphate
show the reaction diagram
CTP + myo-inositol
CDP + myo-inositol 1-phosphate
show the reaction diagram
GTP + myo-inositol
GDP + myo-inositol 1-phosphate
show the reaction diagram
UTP + myo-inositol
UDP + myo-inositol 1-phosphate
show the reaction diagram
additional information
?
-
-
no activity with glycerol, DL-threitol, L-iditol, dulcitol, volemitol, D-lactitol monohydrate, maltitol, D-glucosamine, N-acetyl-D-glucosamine, maltose and lactose, D-erythrose, D-threose, D-erythrulose, L-arabinose, L-fructose, L-glucose, D-ribulose, D-galactose, fructose 1-phosphate, fructose 6-phosphate, glucose 1-phosphate, glucose 6-phosphate, ribose 1-phosphate, ribose 5-phosphate, tagatose 6-phosphate, adenosine, uridine, guanosine, cytidine, thymidine, and inosine
-
-
-
top print hide
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + myo-inositol
ADP + 1D-myo-inositol 3-phosphate
show the reaction diagram
ATP + myo-inositol
ADP + myo-inositol 1-phosphate
show the reaction diagram
-
biosynthesis of phytic acid
-
-
-
top print hide
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
133% activity at 3 mM
Mg2+
-
required for activity
Zn2+
-
125% activity at 3 mM
top print hide
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
-
49% residual activity at 3 mM
Mn2+
-
21% residual activity at 3 mM
Ni2+
-
53% residual activity at 3 mM
top print hide
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0531
1D-myo-inositol
-
in 20 mM HEPES (pH 7.2), 6 mM MgCl2, 10 mM LiCl, 1 mM dithiothreitol, at 30°C
0.036
ATP
-
in 20 mM HEPES (pH 7.2), 6 mM MgCl2, 10 mM LiCl, 1 mM dithiothreitol, at 30°C
863
D-Allose
-
at pH 7.5 and 85°C
171
D-Lyxose
-
at pH 7.5 and 85°C
951
D-ribose
-
at pH 7.5 and 85°C
53
D-tagatose
-
at pH 7.5 and 85°C
438
D-talose
-
at pH 7.5 and 85°C
418
D-xylose
-
at pH 7.5 and 85°C
524
D-xylulose
-
at pH 7.5 and 85°C
0.36
myo-inositol
-
at pH 7.5 and 85°C
top print hide
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
18.3
D-Allose
-
at pH 7.5 and 85°C
3.9
D-Lyxose
-
at pH 7.5 and 85°C
5.1
D-ribose
-
at pH 7.5 and 85°C
1.5
D-tagatose
-
at pH 7.5 and 85°C
13.1
D-talose
-
at pH 7.5 and 85°C
5.6
D-xylose
-
at pH 7.5 and 85°C
11.6
D-xylulose
-
at pH 7.5 and 85°C
23.2
myo-inositol
-
at pH 7.5 and 85°C
top print hide
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.021
D-Allose
-
at pH 7.5 and 85°C
0.023
D-Lyxose
-
at pH 7.5 and 85°C
0.005
D-ribose
-
at pH 7.5 and 85°C
0.028
D-tagatose
-
at pH 7.5 and 85°C
0.03
D-talose
-
at pH 7.5 and 85°C
0.013
D-xylose
-
at pH 7.5 and 85°C
0.022
D-xylulose
-
at pH 7.5 and 85°C
64.9
myo-inositol
-
at pH 7.5 and 85°C
top print hide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.063
-
with meso-erythritol as substrate, at pH 7.5 and 85°C
0.098
-
with L-arabitol as substrate, at pH 7.5 and 85°C
0.17
-
with cis-inositol as substrate, at pH 7.5 and 85°C
0.24
-
with L-chiro-inositol as substrate, at pH 7.5 and 85°C
0.39
-
with adonitol as substrate, at pH 7.5 and 85°C
0.5
-
with D-arabitol as substrate, at pH 7.5 and 85°C
1.06
-
with myo-inositol 1-phosphate as substrate, at pH 7.5 and 85°C
1.91
-
with allo-inositol as substrate, at pH 7.5 and 85°C
17.64
-
with scyllo-inositol as substrate, at pH 7.5 and 85°C
34.92
-
with D-chiro-inositol as substrate, at pH 7.5 and 85°C
44.27
-
with myo-inositol as substrate, at pH 7.5 and 85°C
top print hide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.7 - 8.7
-
approx. 25% of maximal activity at pH 6.7, approx. 75% of maximal activity at pH 8.7
top print hide
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
top print hide
PDB
SCOP
CATH
UNIPROT
ORGANISM
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1);
top print hide
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
top print hide
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 30300, calculated from amino acid sequence
top print hide
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
complexed with an ATP analogue and myo-inositol, hanging drop vapor diffusion method, using
-
sitting drop vapor diffusion method, usingv 18-23% (w/v) polyethylene glycol 3350 and 0.2 M NH4I
-
top print hide
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65 - 95
-
the enzyme is extremely thermostable with half-lives of 3 h at 95°C, 19 h at 85°C, 40 h at 75°C, and 88 h at 65°C
top print hide
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, density-gradient centrifugation, partial purification
-
ammonium sulfate, partial purification
Resource Q column chromatography, Resource PHE hydrophobic column chromatography, and Superdex 200 gel filtration
-
top print hide
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL and Rosetta2(DE3)pLysS cells
-
top
top
top
LINKS TO OTHER DATABASES (specific for EC-Number 2.7.1.64)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)