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Information on EC 2.7.1.64 - inositol 3-kinase
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2.7.1.64 inositol 3-kinaseSpecify your search results
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
inositol 1-kinase, kinase, inositol 1- (phosphorylating), MI3K, MIK, myo-inositol 3-kinase, myo-inositol kinase, myo-inositol-3-kinase, myoinositol kinase, TK2285 protein, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
myo-inositol 3-kinase
myo-inositol kinase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + myo-inositol = ADP + 1D-myo-inositol 3-phosphate
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PATHWAY SOURCE
PATHWAYS
-
,
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SYSTEMATIC NAME
IUBMB Comments
ATP:myo-inositol 1-phosphotransferase
-
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CAS REGISTRY NUMBER
COMMENTARY
37278-07-2
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + myo-inositol
ADP + 1D-myo-inositol 3-phosphate
-
-
-
?
ATP + myo-inositol
ADP + myo-inositol 1-phosphate
-
biosynthesis of phytic acid
-
-
?
CTP + myo-inositol
CDP + myo-inositol 1-phosphate
-
weak activity
-
?
CTP + myo-inositol
CDP + myo-inositol 1-phosphate
-
weak activity
-
?
CTP + myo-inositol
CDP + myo-inositol 1-phosphate
-
weak activity
-
?
GTP + myo-inositol
GDP + myo-inositol 1-phosphate
-
no actiity with TTP
-
?
GTP + myo-inositol
GDP + myo-inositol 1-phosphate
-
no actiity with TTP
-
?
GTP + myo-inositol
GDP + myo-inositol 1-phosphate
-
no actiity with TTP
-
?
GTP + myo-inositol
GDP + myo-inositol 1-phosphate
-
no actiity with TTP
-
?
additional information
?
-
no activity with glycerol, DL-threitol, L-iditol, dulcitol, volemitol, D-lactitol monohydrate, maltitol, D-glucosamine, N-acetyl-D-glucosamine, maltose and lactose, D-erythrose, D-threose, D-erythrulose, L-arabinose, L-fructose, L-glucose, D-ribulose, D-galactose, fructose 1-phosphate, fructose 6-phosphate, glucose 1-phosphate, glucose 6-phosphate, ribose 1-phosphate, ribose 5-phosphate, tagatose 6-phosphate, adenosine, uridine, guanosine, cytidine, thymidine, and inosine
-
-
?
additional information
?
-
-
no activity with glycerol, DL-threitol, L-iditol, dulcitol, volemitol, D-lactitol monohydrate, maltitol, D-glucosamine, N-acetyl-D-glucosamine, maltose and lactose, D-erythrose, D-threose, D-erythrulose, L-arabinose, L-fructose, L-glucose, D-ribulose, D-galactose, fructose 1-phosphate, fructose 6-phosphate, glucose 1-phosphate, glucose 6-phosphate, ribose 1-phosphate, ribose 5-phosphate, tagatose 6-phosphate, adenosine, uridine, guanosine, cytidine, thymidine, and inosine
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + myo-inositol
ADP + myo-inositol 1-phosphate
-
biosynthesis of phytic acid
-
-
?
ATP + myo-inositol
ADP + 1D-myo-inositol 3-phosphate
-
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Adenocarcinoma of Lung
Alternate paths from epidermal growth factor receptor to Akt in malignant versus nontransformed lung epithelial cells: ErbB3 versus Gab1.
Breast Neoplasms
MICAL1 facilitates breast cancer cell proliferation via ROS-sensitive ERK/cyclin D pathway.
Breast Neoplasms
Phosphatidyl inositol 3-kinase (PI3K)-mTOR inhibitor PKI-402 inhibits breast cancer induced osteolysis.
Breast Neoplasms
Targeting the Phosphatidyl Inositol 3-Kinase Signaling Pathway in Breast Cancer.
Breast Neoplasms
The phosphatidyl inositol 3-kinase signaling network: implications for human breast cancer.
Carcinogenesis
Inhibition of midkine by metformin can contribute to its anticancer effects in malignancies: A proposal mechanism of action of metformin in context of endometrial cancer prevention and therapy.
Colorectal Neoplasms
FOXQ1 promotes cancer metastasis by PI3K/AKT signaling regulation in colorectal carcinoma.
Diabetes Mellitus, Type 1
Tuning in to the "right" calcium channel regulation in experimental models of diabetes.
Encephalomyelitis
Suppression of CD4+ T lymphocyte activation in vitro and experimental encephalomyelitis in vivo by the phosphatidyl inositol 3-kinase inhibitor PIK-75.
Fetal Growth Retardation
[Study on the mechanism of insulin resistance in rats born with intrauterine growth retardation]
Infections
Old world arenaviruses enter the host cell via the multivesicular body and depend on the endosomal sorting complex required for transport.
Insulin Resistance
The Mechanism by Which Amentoflavone Improves Insulin Resistance in HepG2 Cells.
Insulin Resistance
[Study on the mechanism of insulin resistance in rats born with intrauterine growth retardation]
Lymphoma
B-cell activating factor-receptor specific activation of tumor necrosis factor receptor associated factor 6 and the phosphatidyl inositol 3-kinase pathway in lymphoma B cells.
Lymphoma
Exosomes from Dendritic Cells Loaded with Chaperone-Rich Cell Lysates Elicit a Potent T Cell Immune Response Against Intracranial Glioma in Mice.
Lymphoma
Sphingosine kinase-1 enhances endothelial cell survival through a PECAM-1-dependent activation of PI-3K/Akt and regulation of Bcl-2 family members.
Lymphoma, B-Cell
Shikonin inhibits TNF-?-induced growth and invasion of rat aortic vascular smooth muscle cells.
Lymphoma, B-Cell
Sphingosine kinase-1 enhances endothelial cell survival through a PECAM-1-dependent activation of PI-3K/Akt and regulation of Bcl-2 family members.
Melanoma
The phosphatidyl inositol 3-kinase pathway is central to the pathogenesis of Kit-activated melanoma.
Neoplasms
?-Estradiol Enhanced Secretion of Lipoprotein Lipase from Mouse Mammary Tumor FM3A Cells.
Neoplasms
Association between Genetic and Immunological Background of Hepatocellular Carcinoma and Expression of Programmed Cell Death-1.
Neoplasms
B-cell activating factor-receptor specific activation of tumor necrosis factor receptor associated factor 6 and the phosphatidyl inositol 3-kinase pathway in lymphoma B cells.
Neoplasms
Differential radio-adaptive responses in BALB/c and C57BL/6 mice: pivotal role of calcium and nitric oxide signalling.
Neoplasms
Expression, localization, and phosphorylation of Akt1 in benign and malignant thyroid lesions.
Neoplasms
Influence of hepatic artery occlusion on tumor growth and metastatic potential in a human orthotopic hepatoma nude mouse model: Relevance of epithelial-mesenchymal transition.
Neoplasms
Inhibition of midkine by metformin can contribute to its anticancer effects in malignancies: A proposal mechanism of action of metformin in context of endometrial cancer prevention and therapy.
Neoplasms
INPP4B exerts a dual function in the stemness of colorectal cancer stem-like cells through regulating Sox2 and Nanog expression.
Neoplasms
INPP4B inhibits cell proliferation, invasion and chemoresistance in human hepatocellular carcinoma.
Neoplasms
Lupeol modulates NF-kappaB and PI3K/Akt pathways and inhibits skin cancer in CD-1 mice.
Neoplasms
Quercetin enhances susceptibility to NK cell-mediated lysis of tumor cells through induction of NKG2D ligands and suppression of HSP70.
Neoplasms
Using Molecular Docking Analysis to Discovery Dregea sinensis Hemsl. Potential Mechanism of Anticancer, Antidepression, and Immunoregulation.
Neoplasms
Wortmannin induces MCF-7 breast cancer cell death via the apoptotic pathway, involving chromatin condensation, generation of reactive oxygen species, and membrane blebbing.
Osteolysis
Phosphatidyl inositol 3-kinase (PI3K)-mTOR inhibitor PKI-402 inhibits breast cancer induced osteolysis.
Osteosarcoma
Long non-coding RNA CCAT1/miR-148a axis promotes osteosarcoma proliferation and migration through regulating PIK3IP1.
Ovarian Neoplasms
[Effects of PTEN over-expression on phosphatidyl inositol 3-kinase/protein kinase B signal pathway in ovarian epithelial cancer cells].
Prostatic Neoplasms
The role of fibroblast growth factors and their receptors in prostate cancer.
Status Epilepticus
Expression of Bcl-2 and Bad in hippocampus of status epileptic rats and molecular mechanism of intervened recombinant human erythropoietin.
Thymoma
FOXQ1 promotes cancer metastasis by PI3K/AKT signaling regulation in colorectal carcinoma.
Thyroid Neoplasms
Suppressor of cytokine signaling 3 sensitizes anaplastic thyroid cancer to standard chemotherapy.
Triple Negative Breast Neoplasms
[Molecular mechanisms of resistance to phosphatidyl inositol 3-kinase inhibitors in triple-negative breast cancer cells].
Tuberculosis
Regulation of matrix metalloproteinase-1, -3, and -9 in Mycobacterium tuberculosis-dependent respiratory networks by the rapamycin-sensitive PI3K/p70S6K cascade.
Whooping Cough
Signal transduction pathways involved in soluble fractalkine-induced monocytic cell adhesion.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0531
1D-myo-inositol
-
in 20 mM HEPES (pH 7.2), 6 mM MgCl2, 10 mM LiCl, 1 mM dithiothreitol, at 30°C
0.036
ATP
-
in 20 mM HEPES (pH 7.2), 6 mM MgCl2, 10 mM LiCl, 1 mM dithiothreitol, at 30°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
1.06
with myo-inositol 1-phosphate as substrate, at pH 7.5 and 85°C
34.92
with D-chiro-inositol as substrate, at pH 7.5 and 85°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.8
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.7 - 8.7
-
approx. 25% of maximal activity at pH 6.7, approx. 75% of maximal activity at pH 8.7
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). MIK_ARATH
353
0
38646
Swiss-Prot
Mitochondrion (Reliability: 5)
MIK_MAIZE
379
0
39898
Swiss-Prot
other Location (Reliability: 1)
MIK_ORYSJ
397
0
41540
Swiss-Prot
other Location (Reliability: 2)
A0A2P6PS81_ROSCH
164
1
18239
TrEMBL
Mitochondrion (Reliability: 3)
A0A2P6QJX7_ROSCH
385
0
42092
TrEMBL
Mitochondrion (Reliability: 3)
A0A2P6P813_ROSCH
139
0
15261
TrEMBL
Mitochondrion (Reliability: 2)
A0A2P6PZ89_ROSCH
58
0
6326
TrEMBL
Mitochondrion (Reliability: 3)
A0A2P6PIK1_ROSCH
90
0
10192
TrEMBL
other Location (Reliability: 3)
G7KE51_MEDTR
372
0
40686
TrEMBL
other Location (Reliability: 2)
Q5JDA3_THEKO
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
273
0
30309
TrEMBL
-
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
complexed with an ATP analogue and myo-inositol, hanging drop vapor diffusion method, using
sitting drop vapor diffusion method, usingv 18-23% (w/v) polyethylene glycol 3350 and 0.2 M NH4I
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
65 - 95
the enzyme is extremely thermostable with half-lives of 3 h at 95°C, 19 h at 85°C, 40 h at 75°C, and 88 h at 65°C
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, density-gradient centrifugation, partial purification
-
ammonium sulfate, partial purification
Resource Q column chromatography, Resource PHE hydrophobic column chromatography, and Superdex 200 gel filtration
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL and Rosetta2(DE3)pLysS cells
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
English, P.D.; Dietz, M.; Albersheim, P.
Myoinositol kinase: partial purification and identification of product
Science
151
198-199
1966
Vigna radiata var. radiata, Spinacia oleracea, Triticum aestivum
brenda
Molinari, E.; Hoffmann-Ostenhof, O.
Studies on the biosynthesis of cyclitols. XXI. An enzyme system capable of phosphorylating myo-inositol to phytic acid
Hoppe-Seyler's Z. Physiol. Chem.
349
1797-1799
1968
Lemna gibba
brenda
Loewus, M.W.; Sasaki, K.; Leavitt, A.L.; Munsell, L.; Sherman, W.R.; Loewus, F.A.
Enantiomeric form of myo-inositol-1-phosphate produced by myo-inositol-1-phosphate synthase and myo-inositol kinase in higher plants
Plant Physiol.
70
1661-1663
1982
Triticum aestivum
brenda
Dietz, M.; Albersheim, P.
The enzymic phosphorylation of myo-inositol
Biochem. Biophys. Res. Commun.
19
598-603
1965
Acer pseudoplatanus, Bos taurus, Escherichia coli, Vigna radiata var. radiata
brenda
Shi, J.; Wang, H.; Hazebroek, J.; Ertl, D.S.; Harp, T.
The maize low-phytic acid 3 encodes a myo-inositol kinase that plays a role in phytic acid biosynthesis in developing seeds
Plant J.
42
708-719
2005
Zea mays
brenda
Nagata, R.; Fujihashi, M.; Sato, T.; Atomi, H.; Miki, K.
Crystal structure and product analysis of an archaeal myo-inositol kinase reveal substrate recognition mode and 3-OH phosphorylation
Biochemistry
54
3494-3503
2015
Thermococcus kodakarensis (Q5JDA3), Thermococcus kodakarensis
brenda
Sato, T.; Fujihashi, M.; Miyamoto, Y.; Kuwata, K.; Kusaka, E.; Fujita, H.; Miki, K.; Atomi, H.
An uncharacterized member of the ribokinase family in Thermococcus kodakarensis exhibits myo-inositol kinase activity
J. Biol. Chem.
288
20856-20867
2013
Thermococcus kodakarensis (Q5JDA3), Thermococcus kodakarensis
brenda
Moure, M.J.; Zhuo, Y.; Boons, G.J.; Prestegard, J.H.
Perdeuterated and 13C-enriched myo-inositol for DNP assisted monitoring of enzymatic phosphorylation by inositol-3-kinase
Chem. Commun. (Camb.)
53
12398-12401
2017
Thermococcus kodakarensis
brenda
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