Information on EC 2.7.1.62 - phosphoramidate-hexose phosphotransferase

for references in articles please use BRENDA:EC2.7.1.62
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The expected taxonomic range for this enzyme is: Escherichia coli

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EC NUMBER
COMMENTARY hide
2.7.1.62
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RECOMMENDED NAME
GeneOntology No.
phosphoramidate-hexose phosphotransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
phosphoramidate + D-hexose = NH3 + alpha-D-hexose 1-phosphate
show the reaction diagram
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
-
-
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SYSTEMATIC NAME
IUBMB Comments
phosphoramidate:D-hexose 1-phosphotransferase
Activity is observed with several hexoses; of these glucose is the best substrate and the product from it is alpha-D-glucose 1-phosphate. The phosphoramidate donor can be replaced by N-phosphoglycine and by an N-phosphohistidine. May be identical with EC 3.1.3.9 glucose-6-phosphatase.
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CAS REGISTRY NUMBER
COMMENTARY hide
9031-45-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Crookes strain, ATCC 8739
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Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
phosphoramidate + 2-deoxy-D-glucose
2-deoxy-D-glucose 1-phosphate + NH3
show the reaction diagram
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-
-
-
?
phosphoramidate + 3-O-methylglucose
3-O-methylglucose 1-phosphate + NH3
show the reaction diagram
-
-
-
-
?
phosphoramidate + D-glucose
D-glucose 1-phosphate + NH3
show the reaction diagram
phosphoramidate + fructose
fructose 1-phosphate + NH3
show the reaction diagram
-
-
-
?
phosphoramidate + glucosamine
glucosamine 1-phosphate + NH3
show the reaction diagram
-
-
-
-
?
phosphoramidate + mannose
mannose 1-phosphate + NH3
show the reaction diagram
-
-
-
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phosphoramidate + D-glucose
D-glucose 1-phosphate + NH3
show the reaction diagram
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phosphoramidate, i.e. PNH2, is delivered by KO3PNH3
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-
-
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
F-
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1.5 mM, at pH 8.7 8% inhibition, at pH 6.5 90% inhibition
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
12.5
glucose
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37°C, pH 8.7
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22.7
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37°C, pH 8.7
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.7
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assay at
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
-
pH 7.5, more than 30 min, no loss of activity
100
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10 min, about 60% loss of activity
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C or -15°C, 6 years stable
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 2.7.1.62)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)